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Citations for Roche :
7651 - 7700 of 8748 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Inhibitors were added fresh on the day of use and included 1 cOmplete™ protease inhibitor cocktail EDTA free tablet (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail 3 both purchased from Sigma-Aldrich (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested and resuspended in lysis buffer A (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM PMSF, and Roche EDTA free protease inhibitor). Following sonication and centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... The obtained cDNAs were diluted 10 times in water and quantitative PCR was performed using 2.5 μL of each diluted cDNA in a total volume of 10 μL containing 1× LightCycler 480 SYBR Green I Master Mix (Roche Diagnostics, cat. #04707516001) and 800 nM of each primer in a LightCycler 480 System (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2021Quote: ... and the cDNA template was digested for 30 min at 37 °C after the addition of 1 µl DNase I (10 U/µl; Roche Diagnostics, Rotkreuz, Switzerland), 5 µl 1M Tris/HCl buffer (pH 8.0) ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with high salt HKT buffer (25mM HEPES pH 7.2, 1M NaCl, 1% Triton X-100, 2mM DTT, 1x Protease Inhibitor (Roche, cOmplete™ EDTA free)) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were collected and incubated in HKT lysis buffer (25mM HEPES pH 7.2, 150mM KCl, 1% Triton X-100, 2mM DTT, 1x Protease Inhibitor (Roche, cOmplete™ EDTA free)) for 30 minutes on ice prior to homogenization with a 25-gauge syringe needle ...
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bioRxiv - Cell Biology 2022Quote: Cells were washed once with ice-cold PBS and stored at -80 °C or immediately lysed using lysis buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% NP-40, 2x Roche EDTA-free protease inhibitors). Lysates were collected by scraping and were cleared by centrifugation at 16,100 g for 10 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Plant Biology 2021Quote: ... total protein was isolated from Agrobacterium infiltrated leaves using extraction buffer (50mM Tris-Cl, 1% β-Mercaptoethanol and protease inhibitor cocktail (Roche, Basel, Switzerland)) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by mechanical and enzymatic digestion with 150 μL collagenase/dispase (1 mg/mL) and 300 μL DNAse (10 mg/mL; both Roche diagnostics, IN, USA) for 45 min at 37 °C with mild agitation ...
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bioRxiv - Biochemistry 2020Quote: ... The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol, 10 mM Tris-HCl pH 7.4) containing protease inhibitors (complete protease inhibitor cocktail, Roche; 1 tablet per 50 mL), and lysed by 10 strokes in a Dounce homogenizer ...
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bioRxiv - Genetics 2022Quote: ... Samples were suspended in Laemmli sample buffer supplemented with a protease and phosphatase inhibitor cocktail (PIC; 1 mM PMSF, cOmplete™ Protease Inhibitor Cocktail [Roche, Base, Switzerland], PhosSTOP [Roche, Base, Switzerland]), and 10 mM N-ethyl maleimide (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with cold PBS and resuspended in NE1 buffer (20 mM HEPES-KOH pH7.9, 10 mM KCl, 1mM MgCl2, 1 mM DTT, 0.1% triton X-100 supplemented with Roche complete protease inhibitor EDTA-free) and rotated for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was then incubated in antibody detection solution consisting of 1:20000 anti-HA rat mAb conjugated to horseradish peroxidase (HRP) (Roche [3F10], Cat. # 1201381900) diluted in MTBST 0.05% for 1 hour at room temperature on a tube rotator ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in IP buffer (150 mM NaCl, 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... The cDNA was diluted 20-fold and used for qRT-PCR employing a LightCycler® 480 SYBR Green 1 Master PCR labelling kit (Roche Applied Sciences) and RotorGene 3000 Real time PCR machine (Corbett Research ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription of 1 µg RNA into cDNA was done using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Cell Biology 2019Quote: Exponentially grown yeast cell cultures were pelleted and resuspended in yeast extraction buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA) with 1x protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany). Cells were lysed by vortexing them together with glass beads (3-fold amount of glass beads and extraction buffer to pellet weight ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice using ice-cold D-PBS before scraping in ice-cold RIPA lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40 alternative, complete protease inhibitor and phosphatase inhibitor [Roche Applied Science, Mannheim, Germany]). The lysate was incubated on ice for 30 minutes with gradual agitation before clarification of samples by centrifugation at 10,000 ×g for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Pellets from exponentially grown cultures of Pea2-9myc expressing yeast cells were resuspended in yeast extraction buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA) with 1x protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany), and vortexed together with glass beads (3-fold amount of glass beads and extraction buffer to pellet weight ...
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bioRxiv - Cancer Biology 2021Quote: ... one 10 cm plate of Hepa1c1c7 cells at 80% confluence was lysed in 1 mL Pierce IP Lysis Buffer (supplemented with 100 U/mL SUPERase-IN and 1X Roche protease inhibitor cocktail) and incubated on ice for 10 min ...
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bioRxiv - Physiology 2020Quote: ... and immunodetection of the DIG-labeled hybrids was performed with a peroxidase-conjugated anti-DIG antibody (1:2000; raised in sheep; Roche Diagnostics, Mannheim, GER). The fluorescence signal was developed with tyramide signal amplification (TSA ...
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bioRxiv - Developmental Biology 2019Quote: ... sections underwent a series of saline sodium citrate buffer washes and were incubated with the anti-DIG-AP fab fragments (Roche, 11093274910, 1:1000) O/N at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... The final pellet was resuspended in 150 μl of NTE buffer (137 mM NaCl, 1 mM EDTA, 10 mM Tris, pH 7.4) with protease inhibitor (Roche, cOmplete Mini tablets, 4693159001). In ultracentrifugation library experiments ...
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bioRxiv - Cell Biology 2019Quote: ... transferred to nitrocellulose (Hybond-ECL, GE Bioscience, Chalfont St.Giles, UK) and incubated for 1 hour in Western blocking reagent (Roche Applied Science, Basel, Switzerland). Primary antibodies (anti-human IL-17RA antibody ...
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bioRxiv - Cell Biology 2020Quote: ... and then digested for 1 h at 37°C in a vigorously shaking solution consisting of 16 mg of Collagenase D (Roche, Pleasanton, CA, USA), 3.0 U of Dispase II (Roche) ...
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bioRxiv - Neuroscience 2021Quote: Cells were washed in cold D-PBS prior to lysis on ice using lysis buffer (1% NP40, 100mM Tris pH8, 100mM NaCl, 10% glycerol, 5mM EDTA, cOmplete™ EDTA-free Protease Inhibitor Mixture, Roche Applied Science) supplemented with 20 mM N-ethylmaleimide ...
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bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s instruction and 1 microG of total RNA was reverse transcribed into cDNAs using the first-strand synthesis kit (Roche, Tucson, AZ, USA). Real-time quantitative PCR analyses were performed according to a previously published procedure 39 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 mM pyrophosphate, 40 mM Hepes pH 7.4, 2.5 mM MgCl2 and 1 tablet of EDTA-free protease inhibitor [Roche] (per 25 ml of buffer)) ...
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bioRxiv - Immunology 2021Quote: ... Lungs were mechanically dissociated by using a scalpel and transferred to collagenase solution consisting of DMEM supplemented with collagenase D (Roche, 1 mg/ml) and DNase1 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... sections underwent a series of saline sodium citrate buffer washes and were incubated with anti-DIG-AP fab fragments (Roche, 11093274910, 1:1000) O/N at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and washed once with HK buffer (25mM HEPES pH 7.2, 150mM KCl, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, EDTA-free cOmplete tabTM)) ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was aspirated and the pellet was resuspended in a pre-warmed digestion mix containing 1 mg ml-1collagenase/dispase (Roche Diagnostics, Indianapolis, USA) and 10 μg ml-1DNase I (Roche Diagnostics ...
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bioRxiv - Cell Biology 2022Quote: ... Heart tissue was homogenized on ice using Tissue Tearor at medium speed for 1 minute in lysis buffer (RIPA plus 0.1% SDS with Roche EDTA-free protease inhibitor) and allowed to sit on ice 10 minutes followed by sonication using Branson sonifier at 10% amplitude ...
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bioRxiv - Cell Biology 2024Quote: ... 4 s off for 1 min at power 10 microns of amplitude) in ice-cold buffer (50 mM Hepes pH 7.5, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and spun down at 13,000 g at 4 °C for 15 min ...
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bioRxiv - Genomics 2024Quote: ... the tissue fragments were transferred into a Douncer homogenizer containing a protease inhibitor cocktail (PIC, Roche, 1 tablet in 50 ml of PBS) solution immersed in ice and homogenized using pestles A and B (from less to more plunger adjustment) ...
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bioRxiv - Immunology 2024Quote: ... Brains were homogenized using a glass Potter and digested for 45 min at room temperature (RT) in Hanks’ balanced salt solution (HBSS) medium with collagenase D (1 mg/ml, Roche Diagnostics cat # 11088882001) and deoxyribonuclease (DNase ...
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bioRxiv - Microbiology 2024Quote: ... samples were collected and plasma HIV RNA levels tested by quantitative rRT-PCR (Roche Cobas Ampliprep/Cobas Taqman HIV-1 test, Roche Diagnostics, Mannheim, Germany) at the NICD.
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bioRxiv - Genomics 2023Quote: ... DNA-Seq libraries were prepared from 1 mg of DNA extracted from young leaves using the Kapa LTP library prep kit (Kapa Biosystems, MA, USA). After quantity and quality evaluation with the High Sensitivity chip of a Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... both vascular and parenchymal pellets were resuspended in cold 1% NP40 in PBS buffer with protease and phosphatase inhibitors added (Roche 04693159001 and 04906837001). The resuspended pellets were then vortexed for 20 s ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then mounted to glass slides and coverslipped with Vectashield Vibrance (Vector H-1700) Primary antibodies used for immunohistochemistry (IHC) are listed here with dilutions indicated in parentheses: rat anti-HA (Roche 11867423001, 1:500), chicken anti-GFP (Abcam ab13970 1:1500) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein expression was determined by solubilizing 2–4 x 106 cells in lysis buffer (1% Triton X-100, 150 mM NaCl, 0.5 mM EDTA) plus 2X protease inhibitor cocktail (Roche Applied Science, Boulder, CO). Samples were analyzed by SDS-PAGE and Western analysis was performed using either mouse anti-FLAG antibody (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... a left lobe was digested for 1 hour at 37°C in RPMI containing 13 mg/mL DNase I (Roche, Randburg, South Africa) and 50 U/mL collagenase IV (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in anti-DIG POD (sheep anti-digoxigenin peroxidase conjugated antibody, RRID AB_514500; Roche, diluted 1:200 in the blocking buffer) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Pellets were resuspended in lysis buffer (20mM Tris-HCL pH 8.0, 300mM NaCl; 4ml/100ml culture) with freshly added protease inhibitors (Roche #11697498001: 1 tablet/50ml) and lysozyme (0.1mg/ml) ...
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bioRxiv - Developmental Biology 2023Quote: ... Pelleted nuclei were resuspended in 333 µl of nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS, 1x Roche Complete protease inhibitors cocktail), kept 5 min on ice and diluted with 667 µl of ChIP dilution buffer (16.7 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: RNA was obtained from epithelioids 1 week after medium change to mFAD or from mouse esophageal epithelium peeled and incubated with Dispase I (Roche catalog no. 04942086001) diluted at 1 mg/ml in PBS for 15 min ...
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bioRxiv - Immunology 2023Quote: ... The gut was then added to a 1.5 mL Eppendorf tubed filled with 1 mL pre-warmed 37°C collagenase/dispase solution (Roche 10 269 638 001) and incubated for 90 seconds before adding to a 40-micron filter as above ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM Na-β-Glycerophosphate and 1% (v/v) TritonX-100 and immediately prior to use the buffer was supplemented with cOmplete™ protease inhibitor cocktail (Roche. Cat# 04693116001).