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Citations for Roche :
7451 - 7500 of 7615 citations for 7 Methylimidazo 1 2 a pyridine 3 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Viral loads were measured at all study visits using the Roche Cobas Taqman HIV-1 Test v2.0 (Roche Diagnostics, Branchburg, NJ, USA). CD4 T cell counts were also enumerated at all study visits using the 4-colour MultiTEST/Trucount assay (Becton Dickinson ...
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bioRxiv - Molecular Biology 2023Quote: ... were sequenced in the forward direction on 1/8 plate of a 454 Life Sciences Genome Sequencer FLX machine (Roche, Branford, CT) using the Macrogen facilities (South Korea).
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bioRxiv - Developmental Biology 2023Quote: ... The qPCR was performed using PerfectStartTM Green qPCR Super Mix (TransGen Biotech, 1) on a Real-time PCR Detection System (Roche, LightCycle480 II). RPLP0 served as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... Vero/TMPRSS2 cells (JCRB Cell Bank, Cat# JCRB1818) were cultured in DMEM containing 5% FBS and 1 mg/mL G418 (Roche, Basel, Switzerland). VeroE6/TMPRSS2 cells (JCRB Cell Bank ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with 12 ml of elution buffer (20 mM Tris, 500 mM NaCl, 6 M guanidine, 600 mM imidazole, 1 mM MgCl2 and 1X Roche protease inhibitor) and exchanged into #1 to #5 dialysis buffers in order to remove the imidazole and the Guanidine ...
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bioRxiv - Genomics 2023Quote: ... 3 million crosslinked cells were resuspended in 1 mL of ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP-40, and 1× Roche protease inhibitors Cat#11697498001) and rotated at 4 °C for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... in 5% non-fat dry milk in TBS/0.1% Tween-20 (TBST) or in 1% Western blocking reagent (WBR) (Roche Applied Science, Germany) in TBS (for β-actin) ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in immunoprecipitation buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% TritonX-100 supplemented with Roche protease inhibitor cocktail). Lysates were centrifuged (5,000x g ...
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bioRxiv - Cell Biology 2023Quote: ... Livers were collected on ice and incubated in digestion buffer (0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The cell pellet was washed once with 50 mM Tris-HCl (pH 7.0) and resuspended in 1 mL of 50 mM Tris-HCl (pH 7.0) containing protease inhibitors (cOmplete, Roche Applied Science, Mannheim, Germany). The cells were disrupted with glass beads using a FastPrep system (Qbiogene ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested and either lysed using sonication in HED buffer (25 mM HEPES, 10% glycerol, 1 mM DTT, and EDTA-free protease inhibitor (Roche, Basel, Switzerland)) containing 0.1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007, Roche, Bâle, Switzerland) or anti-β-tubulin mouse antibody (Catalogue number T9026 ...
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bioRxiv - Immunology 2023Quote: ... and PLN from non-diabetic 6w– 8w female mice were minced and enzymatically dissociated with 1 mg/mL collagenase D (Roche Applied Science) and 25 U/mL DNase I in DMEM for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were brought to room temperature and washed twice in TNT before being incubated in blocking buffer (TNT+5% sheep serum+1% Roche blocking reagent) for 1 hour ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with primary antibodies diluted 1:100 (rabbit pAb α-γH2A.X from SigmaAldrich, H5912 and mouse mAb α-GFP from Roche, 11814460001) in 1% BSA (in PBS ...
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bioRxiv - Neuroscience 2023Quote: Lysates from cultured neurons were collected in NP40 lysis buffer (150 mM NaCl, 100 mM Tris-HCl, 1% NP40 in PBS) containing 1X protease inhibitors (cOmplete ULTRA tablets, Roche, cat# 05892970001). Samples were run on mini-protean TGX gels (Biorad ...
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bioRxiv - Immunology 2023Quote: ... Cationic lipid N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (Dotap, Liposomal Transfection Reagent, Cat#1202375, Roche, Nonnenwald, Penzberg, Germany) was used for the conjugation of phosphorothioated miRNAs and Dotap-formulated miRNAs were used for in vitro delivery of miRNAs ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001; clone 3F10, monoclonal from Roche, 1:5000 in 5% milk) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... then treated with blocking reagent (Genostaff) for 15 min and with alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... a maximum of 1 μg of sheared DNA was used for library preparation using the KAPA HTP Prep Kit (KAPA Biosystems, KK8234) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability of cell lines in the presence of increasing concentrations of different drugs was assayed using the cell proliferation reagent WST-1 (Roche, Mannheim, Germany) after 72 h-treatments as previously reported 35 ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 min or TRAP staining with a TRAP/ALP Stain Kit (FUJIFILM Wako Pure Chemical Corporation) for 30 min or ALP staining with NBT/BICP solution (Roche; 1:100) for 15 min followed by AR staining prepared from 1% AR Solution at pH 6.3-6.4 (MUTO PURE CHEMICALS CO. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... dissected samples were homogenized with a pestle in 1% sodium dodecyl sulfate in PBS supplemented with a protease inhibitor cocktail (cOmpleteTM mini, EDTA free, Roche, Basel, Switzerland). The resultant lysate was centrifuged at 20,000× g for 15 min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The probe was detected by incubating samples overnight at 4°C with a 1:1500 dilution of Anti-DIG-POD antibody (Roche, Catalog# 11207733910) in Blocking Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR amplification of a 1:10 dilution of cDNA was performed in triplicate on a LightCycler® 480 II thermal cycler (Roche, France) using the LightCycler® 480 SYBR Green I Master mix and specific primers (Table S2) ...
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bioRxiv - Developmental Biology 2024Quote: ... the extirpated tongues from non- transgenic and Lgr5-EGFP-IRES-CreERT2;R26-mTmG reporter mice were injected with approximately 100-200 μl 1% Dispase II (neutral protease; Roche, Indianapolis, IN) dissolved in Balanced Salt Solution (BSS ...
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bioRxiv - Microbiology 2024Quote: ... the RNA-seq directional libraries were generated for 1 μg rRNA-depleted RNA using the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, KK8421). The rRNA-depleted RNA was fragmented ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Probes were detected by incubation overnight at 4°C in a 1:5000 dilution of Anti-DIG-AP antibody (Roche, Catalog# 11093274910) in Blocking Buffer ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were harvested by centrifugation at 6,000 g for 10 min at 4°C and resuspended in L Buffer (25 mM Tris-HCl, 500 mM NaCl, 10 mM Imidazole, 1 mM PMSF, 5 % Glycerol, pH 8, containing Roche protease inhibitor cocktail). Bacteria were then lysed by sonication ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cell lysates were prepared in radioimmuno-precipitation assay (RIPA) buffer supplemented with 1 tablet of EDTA-free protease inhibitor (Roche, Cat No. C762Q77) per 25ml buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then quickly washed twice in wash+ buffer (20 mM HEPES pH7.5 + 150 mM NaCl + 0.1% BSA + 0.5 mM Spermidine + 1 large Roche complete EDTA-free tablets in water). Samples were resuspended in 150 μl Wash+C (wash+ + 100 mM CaCl2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Pelleted nuclei were resuspended in 333 μl of nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS, 1x Roche Complete protease inhibitors cocktail), kept 5 min on ice and diluted with 667 μl of ChIP dilution buffer (16.7 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... expressing polymerase module and SII-tagged Mpe1 were lysed in Buffer A (50 mM HEPES pH 8, 75 mM NaCl, 1 mM TCEP) supplemented with 3x protease inhibitor tablets (Roche, cat. No. 11836153001) and 1 ml BioLock ...
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bioRxiv - Genetics 2020Quote: ... After 24 hours cells were then fixed with a 1% (v/v) formaldehyde solution supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche®, UK) for 15 minutes with gentle agitation at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Cell-pellets were resuspended in 25 ml PBS and incubated for 30 min at 4 °C in the presence of 1 mg/ml Lysozym and protease inhibitors (Roche Diagnostics, Penzberg, Germany) The cell lysate was cleared by centrifugation at 40000 g for 10 minutes after sonification with a Bandelin Sonapuls HD 2070 (Reichmann Industrieservice ...
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Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: Cells were lysed in Tris-Buffered Saline + 1% Triton X-100 (TBST) with protease and phosphatase inhibitor cocktails (Roche Diagnostics, Florham Park, NJ). To remove insoluble materials ...
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bioRxiv - Cell Biology 2019Quote: ... This was repeated twice followed by incubation for 1 hour at 37°C in a solution containing Collagenase D (Roche; 1.5 U/ml), Dispase II (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was resuspended in 50 ml lysis buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, Roche Complete protease inhibitor cocktail) and the lysate was boiled for 15-20 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Pellets were resuspended in 5 mL of membrane isolation buffer (5 mM TrisHCl pH 7.6, 5 mM MgCl2, 1 mM EGTA, protease inhibitor cocktail cOmplete [Roche Applied Science, Penzberg, Germany]) with a glass dounce tissue grinder (Wheaton ...
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bioRxiv - Molecular Biology 2019Quote: ... 250 mM sucrose, 5 mM MgCl2, 1 mM EGTA, 0.05% Tween-20, 0.5 mM DTT, 1x protease inhibitors [Roche], 0.4 u/μl RNase inhibitor). The nuclei or permeabilized cells were flash-frozen and stored at −80°C (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... 72°C for 1 min) including the TruSeq primer mix and the KAPA Hifi Uracil+ Poly-merase Master Mix (Kapa Biosystems, Cat. #KK2801). The PCR product was purified and size controlled by Agilent Technologies 2100 Bioanalyzer (High Sensitivity DNA Chip) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen) and SOX9 (1:10000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were pelleted in a centrifuge and lysed with lysis buffer (20mM Tris pH 7.5, 1% Triton, 1mg/mL Lysozyme, 5mM DTT and protease inhibitor cocktail, Roche #11-836-170 001) for 1 hour at 4 °C ...
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bioRxiv - Physiology 2021Quote: 50 islets per sample were washed in PBS twice and then lysed and sonicated in RIPA buffer (10mM HEPES, 50mM β-glycerol phosphate, 1% Triton X-100) supplemented with complete mini protease inhibitor cocktail (Roche, Laval, QC) and phosphatase inhibitors (2mM EGTA ...
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bioRxiv - Physiology 2020Quote: ... Inhibitors were added fresh on the day of use and included 1 cOmplete™ protease inhibitor cocktail EDTA free tablet (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail 3 both purchased from Sigma-Aldrich (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... along with the appropriate antibody (mouse HA 12CA5, 7.5 μl 0.4 mg/ml, Roche; mouse M2 FLAG, 5 μl 1 mg/ml, Sigma), before incubating at 4 °C on a rotating wheel at 14 rpm for 15-18 h ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested and resuspended in lysis buffer A (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM PMSF, and Roche EDTA free protease inhibitor). Following sonication and centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... The obtained cDNAs were diluted 10 times in water and quantitative PCR was performed using 2.5 μL of each diluted cDNA in a total volume of 10 μL containing 1× LightCycler 480 SYBR Green I Master Mix (Roche Diagnostics, cat. #04707516001) and 800 nM of each primer in a LightCycler 480 System (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2021Quote: ... and the cDNA template was digested for 30 min at 37 °C after the addition of 1 µl DNase I (10 U/µl; Roche Diagnostics, Rotkreuz, Switzerland), 5 µl 1M Tris/HCl buffer (pH 8.0) ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with high salt HKT buffer (25mM HEPES pH 7.2, 1M NaCl, 1% Triton X-100, 2mM DTT, 1x Protease Inhibitor (Roche, cOmplete™ EDTA free)) for 10 minutes ...