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Citations for Roche :
7251 - 7300 of 7615 citations for 7 Methylimidazo 1 2 a pyridine 3 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Skin biopsy specimens were collected and minced before digestion in 1mL of DMEM with 1 mg/ mL collagenase D (Roche Diagnostics) for 3 hours at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: Cos-1 cells were grown in DMEM supplemented with 10% heat inactivated FCS and transfected with Fugene HD (Roche Applied Science) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 mg/ml bovine serum albumin dissolved in TBST) for 1 h and subsequently incubated with anti-DIG-Fab antibody coupled to alkaline phosphatase (Roche: 11093274910) in blocking solution (1 in 5000 ...
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bioRxiv - Immunology 2021Quote: ... incubated over-night (o/n) at 4°C with primary antibody diluted 1:500 in 5% Bovine Serum Albumin Fraction V (Roche 10735086001) in TBS-T ...
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bioRxiv - Genetics 2019Quote: ... 10mM KCl, 1mM EDTA, 0.1mM activated Na3VO4, 0.5% NP-40, 10% glycerol, 1mM DTT and 1:1000 Roche cOmplete protease inhibitor) after the third wash ...
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bioRxiv - Genomics 2021Quote: ... Pelleted cells were resuspended in 1 ml of Dig-Wash buffer (0.02% digitonin, 20 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1 Roche Complete tablet -EDTA (11873580001) per 50 ml buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Riboprobes were hybridized for 16 hr at 65°C and embryos were incubated with anti-DIG-AP (Roche, dilution 1/4000) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 1 MM EDTA) supplemented with a cocktail of protease inhibitors (Complete Protease Inhibitor without EDTA, Roche Applied Science, Indianapolis, IN, USA) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were incubated with 1:10 TdT buffer for 10 min and subsequently in reaction mixture [0.12% v/v TdT (3333574001, Roche applied science); 0.25% v/v dUTP (11093070910 ...
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bioRxiv - Microbiology 2022Quote: ... amplification was performed in a 20-μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics), 4 mM MgCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 15-20k cells/ well in Cardiac Mesoderm Patterning Media One made up of CDM containing ROCKi and for all protocols besides the LV cardioids 1 µg/ml of insulin (Roche, #11376497001) plus specific factors depending on cardioid subtype (see below) ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was then discarded and the pellet was resuspended in 300 µL SDS buffer (4 % SDS, 10 mM EDTA, 25 mM TrisHCl pH 7.5, 1 mM PMSF, 1x Roche protease inhibitor) and incubated at room temp (10 minutes) ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were rinsed with PBS and incubated with 5% (v/v) normal donkey serum and 1% (w/v) BSA (Roche, 10.7350.86001) in PBS for 1 hour at room temperature to block the non-specific antibody binding ...
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bioRxiv - Bioengineering 2022Quote: ... The optimal cycle numbers for PCR2 were determined for 1 μL of each PCR1 individually by conducting a qPCR amplification using KAPA HiFi HotStart Ready Mix (Roche, 7958927001) and 1× EvaGreen (Biotium ...
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bioRxiv - Bioengineering 2022Quote: ... Encapsulated or adherent cells were plated in a 96 well plate for 24 or 48h when WST-1 reagent (Roche, Switzerland) was added ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at room temperature and incubated with an appropriate antibody overnight at 4°C: 1:3000 anti-DIG-AP (Roche 11093274910), 1:1000 anti-DIG-POD (Roche 11207733910 ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were washed in PBS and resuspended in lysis solution (1xPBS pH 7.4, 1x Roche plant protease Inhibitor, 1 mM PMSF) to a final concentration of 5x108 cells/mL ...
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bioRxiv - Immunology 2022Quote: ... these tissues were dissected and incubated for 1h at 37ºC in the enzymatic cocktail containing Colleagenase D (Roche, 1 mg/ml) and DNAse I (Roche ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed on the plates for 30 minutes at 4ºC in Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, PhosStop, Roche Complete Protease Inhibitor). Lysates were clarified by centrifugation at 13,000 RPM ...
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bioRxiv - Microbiology 2022Quote: ... The total HIV-1 DNA was amplified by quantitative real-time PCR using the Light Cycler instrument (Roche Diagnostics, Meylan, France). Amplification was performed in a 20 μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics) ...
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bioRxiv - Microbiology 2022Quote: ... Amplification was performed in a 20 μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics), 3 mM MgCl2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cells were lysed with tissue lysis buffer supplemented with 0.2 mM sodium vanadate and 1 mM protease inhibitor cocktail (Roche, Basel, Switzerland), and for immunoblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNA (50 μg) was separated on 1% agarose–formaldehyde gels and transferred to Hybond-N+ nylon membranes (Roche, Basel, Switzerland). Northern blotting was conducted with biotin-labeled DNA (bio-CTGTAGAAAGTCTGCTGATCGATACCGCGACG ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed by resuspension in 10mL cold NP-40 lysis buffer (150mM NaCl, 10mM Tris pH 7.2, 1% NP-40, 5mM DTT, 2mM MgCl2) containing protease inhibitors (Roche, Laval, QC) on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and the cell pellet resuspended in 1 ml of PBS with the addition of a protease inhibitor cocktail (cOmplete™ Protease Inhibitor Cocktail, Roche) in order to protect histones from degradation by proteases during the next step of cell lysis ...
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bioRxiv - Microbiology 2022Quote: Single cell suspensions from the ear were obtained by using tweezers to separate the dorsal and ventral sides of the mouse ear and placing the sheets dorsal-side down in a 24 well plate with 1 mL/well of RPMI1640 containing 0.25 mg/mL of Liberase TL (Roche, Diagnostics Corp.) and 10 μg/mL of DNase I (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: Engineered monoallelic cell pellets (500 million cells/sample) were lysed at 4°C in 1% CHAPS (Roche Diagnostics, cat no. 10810126001) lysis buffer (pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with protease and phosphatase inhibitors (1 tablet per 5ml buffer of cOmplete™, mini, EDTA-free protease inhibitor cocktail [Roche] ...
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bioRxiv - Neuroscience 2022Quote: Protein was extracted from iNs by lysis in NP-40 lysis buffer (1% NP40, 0.5M EDTA, 5M NaCl, 1M Tris) containing cOmplete protease inhibitors and phosSTOP (Roche, Penzberg, Germany). Lysates were analyzed using the Multi-Spot Phospho (Thr 231)/Total Tau ELISA (Meso Scale Discovery ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellets were suspended in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Nonidet P-40, 0.5% NaDeoxycholate, 0.1% SDS, 1x Roche protease inhibitor mixture) and sonicated 5 times x 5 min (30 s ON/ 30 s OFF ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing either 40mg/L hygromycin (Roche, no. 10843555001) or 25mg/L DL-phosphinothricin (Duchefa ...
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bioRxiv - Physiology 2024Quote: ... the samples were blocked with 5% skim milk in PBS-DEPC for 1 hour at 4°C and incubated with anti-DIG antibody (Roche Diagnostics) diluted 1:10,000 in the blocking buffer for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended in 880 uL RIPA buffer (1x PBS, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SGS, Roche protease inhibitor cocktail), and sheared using Covaris E220 Focused-Ultrasonicator ...
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bioRxiv - Molecular Biology 2024Quote: The following antibodies and dilutions were used for western blotting or immunofluorescence: horseradish peroxidase-linked anti-HA antibody (Roche, 1:5,000), rabbit anti-INTS4 (Bethyl Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in 400 μL of lysis buffer (50 mM Tris-HCl [pH 8.0], 150 mM NaCl, 1% NP-40, cOmplete EDTA-free protease inhibitor tablets [Roche, Basel, Switzerland]), incubated for 1 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... and cells were lysed on the plates for 30 min at 4 C in Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, PhosStop, Roche Complete Protease Inhibitor). Lysates were clarified by centrifugation at 13,000 RPM ...
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bioRxiv - Microbiology 2024Quote: ... 500 µL of lysis buffer II (Tris-HCl pH 4.7 50 mM, NaCl 150 mM, EDTA 1mM, Triton X100 1%, Roche Antiprotease Cocktail) was added and samples were sonicated for 30’ (Covaris ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extract preparation and immunoblotting of Chlamydomonas cultures were performed as described previously (46, 48) with the following modifications: Antibody dilutions were: rat-anti-HA (1:5000) (3F10, Roche, Switzerland), Anti FLAG rabbit polyclonal (Rockland 600-401-383 ...
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bioRxiv - Pathology 2024Quote: ... The digested products were electrophoresed in 1% (w/v) agarose gel and then transferred onto positively charged Nylon membranes (Roche, Germany). For both assays ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Plant Biology 2024Quote: ... 0.1% Triton X-100, 0.2% Nonidet P-40, 0.6 mM PMSF, and 20 µM MG132 in a Roche protease inhibitor cocktail) was added ...
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bioRxiv - Genetics 2024Quote: ... cut to very small pieces using a scalpel blade and digested using Collagenase D (Roche, Cat No. 11088858001, 1 mg/ml) /DNase I (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used for the preparation of stained gels for expansion microscopy: Rat anti-HA (3F10, 1:25, Roche 12158167001), Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... 20,000g for 20 min and the pellet was resuspended in 50 µl of PBS supplemented with 1 mM TCEP and cOmplete protease inhibitor (Roche, 11836170001). Membrane proteins were solubilized and PEGylated by adding 7 µl of 10% β-d-dodecyl maltoside (Anatrace 329370010 ...
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bioRxiv - Plant Biology 2023Quote: ... 50 mM Tris HCl pH 7.5, 10% glycerol, 10 mM EDTA, 10 mM DTT, 1mM NaF, 1% IGEPAL, 2X protease inhibitor, and 2X Roche phosphatase inhibitor) according to the sample quantity (approximately 200 µl of extraction buffer per 100 mg of ground plant material) ...
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bioRxiv - Systems Biology 2023Quote: ... per sample were washed three times with 1 ml wash buffer (20 mM HEPES at pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, and 1 × Roche complete protease inhibitor) at room temperature and resuspended in 225ul of wash buffer ...
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bioRxiv - Neuroscience 2023Quote: Weighed frozen tissue samples were processed for biochemical assays by adding 5X or 10X volume of chilled 1% NP40 + PBS homogenization buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM NaF) with EDTA-free protease inhibitors (cOmplete™ Tablets) and phosphatase inhibitors (PhosSTOP™, both Roche Diagnostics GmbH, Germany) for 1 hour at 4°C under rotation ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA-seq libraries were then prepared using 1 µg of RNA from each sample and the KAPA RNA HyperPrep Kit with RiboErase (Roche KR1351) according to the manufacturer’s protocol with 10 amplification cycles ...