Labshake search
Citations for Roche :
701 - 750 of 8040 citations for N 5 Trimethoxysilyl 2 Aza 1 Oxopentyl Caprolactam since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... the cells were rinsed with PBS and incubated with 5% (v/v) normal donkey serum and 1% (w/v) BSA (Roche, 10.7350.86001) in PBS for 1 hour at room temperature to block the non-specific antibody binding ...
-
bioRxiv - Bioengineering 2023Quote: ... the cells were rinsed with PBS and incubated with 5% (v/v) normal donkey serum and 1% (w/v) BSA (Roche, 10.7350.86001) in PBS for 1 hour at room temperature to block the non-specific antibody binding ...
-
bioRxiv - Cell Biology 2022Quote: ... 5 mM EDTA, 10 mM β-mercaptoethanol, 10 mM Hepes pH 7.5, supplemented with 1× Complete protease inhibitor cocktail, Roche, Germany), passed through 27 G syringe and left 20 min in ice ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cryosections were then washed 3 times for 5 minutes in PBT before being incubated with 1:10 TUNEL enzyme buffer (Roche #12156792910) at 37°C in the dark for 2 hours ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were ground in liquid nitrogen and homogenized in 40 ml of NEB1 buffer (10 mM Tris-HCl [pH 8.0], 0.4 M sucrose, 10 mM MgCl2, 5 mM DTT, 0.1 mM PMSF, 1% Roche protease inhibitor cocktail tablet). The homogenate was filtrated through two layers of Miracloth ...
-
bioRxiv - Microbiology 2024Quote: ... Then tissue was rinsed three times in ice-cold RPMI-1640 and digested in 5 ml of complete media (RPMI-1640 containing 10% FBS, 1% antibiotic/antimycotic, 5mg/ml DNase I) supplemented with 5 mg/mL Liberase™ (Roche) and 5mg/ml DNase I (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2024Quote: Bacterial cell pellets were resuspended in 50 ml of lysis buffer (50 mM Tris-HCl pH 8, 1 mM DTT, 5% glycerol, protease inhibitor cocktail [Roche 1183615300]). Cells in pellets were lysed by sonication on ice with 10 sec pulses of maximum setting followed by 1 min cooling period ...
-
bioRxiv - Neuroscience 2024Quote: ... slides were blocked with 5% lamb serum diluted in TBST and incubated with an anti-digoxigenin antibody conjugated to Alkaline phosphatase (1/2000, Roche, 11093274910) overnight at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... were harvested by centrifugation and resuspended in 25 ml lysis buffer (20 mM Tris-Hcl, pH 7.5 + 200 mM Nacl + 1 mM DTT + 5% Glycerol) and protease inhibitor (Roche, Basel, Switzerland). The suspended cells were disrupted by sonication and then cell debris was separated by centrifugation at 12,000 rpm for 45 min.
-
bioRxiv - Developmental Biology 2021Quote: ... 2% Blocking reagent (Roche, 11096176001), 0.1% Tween 20 ...
-
bioRxiv - Neuroscience 2020Quote: ... using 2 % blocking reagent (Roche), followed by the detection of the Dig-labelled riboprobe with an anti-DIG Fab fragment conjugated with alkaline phosphatase (1:750 ...
-
bioRxiv - Microbiology 2020Quote: ... 2 U Lactate dehydrogenase (Roche) and 10 µg PGAM was pre-warmed to 30 °C ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2% blocking reagent (Roche – 1096176001), 20% heat-inactivated goat serum and then incubated overnight with anti-DIG-AP antibody (Roche – 11093274910 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2× Protease Inhibitor Cocktail (Roche), 2 mM 2-mercaptoethanol ...
-
bioRxiv - Cell Biology 2021Quote: ... and 2% Nutridoma-CS (Roche) for 6 days39 ...
-
bioRxiv - Biophysics 2020Quote: ... 2 mM creatine phosphate (Roche), 10 ng μL−1 creatine kinase (Roche) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Samples were washed in PBSTr and incubated overnight at 4 °C MABTr/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche, Switzerland) with anti-Fluorescein-POD Fab fragments serum (1:500 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated overnight at 4°C in 0.1M malic acid/0.1%-TritonX (MABTr)/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche, Switzerland) with anti-DIG-AP Fab fragments serum (1:5000 ...
-
bioRxiv - Developmental Biology 2022Quote: ... embryos were incubated overnight at 4 °C in blocking solution MABTr/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche, Switzerland) with anti-DIG-POD Fab fragments serum (1:500 ...
-
bioRxiv - Biochemistry 2023Quote: ... 2 mM creatine phosphate (Roche), 10 µg/ml creatine kinase (Roche) ...
-
bioRxiv - Immunology 2023Quote: ... 2 μg/ml Histon (Roche), 1 μg/ml Sm/RNP (GenWay) ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 mg DNase I (Roche) and triton X-100 to 1% were added ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... 2% blocking reagent (Roche, #11096176001), 10% heat-inactivated sheep serum (Equitech-Bio ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 mg/mL BSA (Roche), 60 µg/mL catalase (Sigma) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 µg BSA (Roche Diagnostics) and 1 U Immolase DNA polymerase (Meridian Bioscience ...
-
bioRxiv - Biochemistry 2023Quote: ... 2 mM GTPgS (Roche, 10220647001) and 4 mM MgCl2 in BRB80 was incubated for 5 hours at 37°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... in 2% Blocking Reagent (Roche) and 5% sheep serum (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 protease inhibitor tablets (Roche), 20 M MG132 (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 PhosSTOP easy Pack (ROCHE), Protease Inhibitor cocktail (Sigma) ...
-
bioRxiv - Microbiology 2022Quote: ... version 2 (Roche, Basel, Switzerland), with a lower limit of detection of 20 copies/ml of plasma.
-
bioRxiv - Biochemistry 2022Quote: ... 2 mM amino acids (Roche), 1 nM pY71sfGFP plasmid encoding superfolder GFP (M ...
-
bioRxiv - Cell Biology 2024Quote: ... 2 U/mL Liberase (Roche) and 0.25 U/mL Elastase (E0127 ...
-
bioRxiv - Cell Biology 2024Quote: ... 2% BSA Fraction V (Roche), 1% P/S solution and 50 μM β-mercaptoethanol ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20mM β-glycerophosphate and a cOmplete™ EDTA-free protease inhibitor cocktail tablet added (Roche, cat n°11873580001). In case of HAP1 lysates ...
-
bioRxiv - Microbiology 2022Quote: Bacterial DNA was extracted and purified using the High Pure PCR Template Preparation kit (Roche, cat. n° 11796828001) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2021Quote: ... After two washes with cold PBS the cells were collected in the Lysis buffer (5 mM PIPES pH 7.5, 85 mM KCl, 0.5% NP40, 20 mM N-ethyl maleimide [NEM] and protease inhibitor cocktail [04693159001, Roche]) and incubated at 4°C for 10 minutes with rotation ...
-
bioRxiv - Immunology 2021Quote: ... in combination with the N-gene (inhouse primer sets in multiplex PCR) on LightCycler® 480 (Roche Diagnostics).
-
bioRxiv - Cell Biology 2024Quote: All qPCR reactions were performed with a LightCyler 1.3 Real-Time PCR system (Roche S/N: 140 6143). The MgCl2 concentration (1-3 mM ...
-
bioRxiv - Immunology 2024Quote: ... colonic biopsies (n=3-4 per patient) were enzymatically digested using 0.5 Wünsch units/ml Liberase TM (Roche) and DNase I at 10µg/ml at 37°C (a protocol previously optimized in our lab)(43) ...
-
bioRxiv - Molecular Biology 2021Quote: T98G cell pellets were fixed by dropwise addition of cold 70% ethanol and their DNA was stained with 4’,6-diamino-2-phenylindole (1 μg/ml, Roche), 0.1% Triton X-100 in phosphate-buffered saline (PBS) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Sections were blocked for 1 h with 2% blocking reagent in MABT (Perkin-Elmer) and then incubated with anti-digoxigenin-POD (Roche), diluted 1:500 ...
-
bioRxiv - Genomics 2020Quote: ... Real-time quantitative PCR reactions were set up in triplicate with 2 μL of cDNA (1:4 dilution) per reaction using the FastStart Universal SYBR Green Master mix (Rox) (Roche) and run on a 7500 Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM Tris(2-carboxy-ethyl) phosphine (TCEP) containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
-
bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with Glutamax and 10 % FBS were transfected using a 2:1 ratio X-treme GENE HP DNA transfection reagent (Roche) per μg of plasmid DNA with a total of 0.5 μg DNA per well ...
-
bioRxiv - Cell Biology 2021Quote: ... 150 mM KOAc, 2 mM MgCl2, 1 mM CaCl2, 0.2 M sorbitol, 10mM PMSF, 2x protease inhibitor cocktails [Roche, 11873580001]), dropped into liquid nitrogen ...
-
bioRxiv - Cancer Biology 2020Quote: ... Transposed chromatin was amplified using customized Nextera PCR Primer 1 and Primer 2 (barcode) (31) and HotStart KAPA ReadyMix (Roche). Libraries were quantified by Qubit using the DS DNA HS kit (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... pH 7.4, 200 mM NaCl, 1% NP-40, 2 mM MgCl2, 10% glycerol, NaF +Na3VO4, complete mini tablets without EDTA, Roche). Lysates were incubated on ice for 15 minutes and centrifuged at 17,000xg at 4°C for 10 minutes ...
-
bioRxiv - Immunology 2022Quote: ... The cells were washed with PBS and crosslinked for 30 min in 2 ml ice cold 1% formaldehyde containing 2X Complete EDTA-free Protease Inhibitor Cocktail (Roche). Cross-linking was stopped by the addition of glycine to 333 mM ...
-
bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche] ...
-
bioRxiv - Biochemistry 2021Quote: ... One mL of yeast lysate (1-2 mg/mL) prepared in column buffer freshly supplemented with protease and DUB inhibitors (cOmplete mini EDTA-free (Roche), 10 mM NEM ...