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Citations for Roche :
501 - 550 of 8040 citations for N 5 Trimethoxysilyl 2 Aza 1 Oxopentyl Caprolactam since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... diluted 10 times and 1 μl was used in 5-μl PCR reaction on a LightCycler 480 (Roche Diagnostics) with Luna Master Mix (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting organoids were further digested with warm 0.25% trypsin for 1 min with constant pipetting followed by treatment with 5 mg/mL Dispase (Roche) for 5 min in at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... bacterial pellets were resuspended using a high salt buffer (25 mM Tris pH7.5, 1 M NaCl, 10 mM imidazole, 5% glycerol) supplemented with protease inhibitor tablets (Roche), 0.5 mM PMSF ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested by centrifugation and resuspended in lysis buffer (50 mM HEPES pH7.5, 2M NaCl, 5 mM MgCl2, 1 tablet of Complete Inhibitor cocktail EDTA Free (Roche) per 50◻ml ...
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bioRxiv - Genomics 2021Quote: ... containing 25 Abs260 ribosome units (1 A260 = 12 μg/μL) [5] were digested with 60 U of MNase (Roche) and 60 U of SUPERase·In (Ambion ...
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bioRxiv - Genomics 2022Quote: ... 25 mM potassium chloride, 5 mM magnesium chloride, 10 mM Tris-HCl pH 8.0, 1 μM DTT, 1x Roche Protease Inhibitor Cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... ground in a fine powder with liquid nitrogen and resuspended with 1 ml of Guanidine hydrochloride extraction buffer (6M GuaHCl, HEPES 1 M, EDTA: 5 Mm, adjusted to pH 7.5) supplemented with protease inhibitor cocktail (Roche). Homogenates were filtered through a 100 µm cotton mesh ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Cells or tissues were washed with PBS and lysed in an appropriate amount of protein lysis buffer (TritonX100 -1%, Tris 7.4 -50mM, EDTA -5mM, NaCl -150mM, and glycerol -5%) containing Protease Inhibitor Cocktail (Roche). We used Phosphatase Inhibitor Cocktail (Millipore ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche) using the following protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were then ground to a fine powder in a mortar and pestle over liquid nitrogen and resuspended in freshly prepared extraction buffer (2mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% v/v NP40, 5% v/v glycerol, 1mM PMSF, 1ml Roche Protease Inhibitor cocktail per 30g input tissue ...
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bioRxiv - Developmental Biology 2024Quote: ... heads were rinsed for 5 minutes in PBST five times and incubated in Blocking Reagent (Roche, 1:5000 dilution) for 60 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Bioengineering 2023Quote: Liver tissue was lysed using RIPA buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, cOmplete® Mini Protease Inhibitor Cocktail [Roche Diagnosis ...
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bioRxiv - Genomics 2023Quote: ... Cells were centrifuged at 500 x g for 5 min at 4C and 500 uL 1X PBS + 1% BSA + DAPI + 0.5U/uL Protector RNAse Inhibitor (Roche) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 500 mM NaCl, 0.4% [wt/vol] SDS, 5 mM EDTA, 1 mM DTT and 1x cOmplete Protease inhibitor cocktail, Roche) for 20 min on a rocker at 4°C ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-stimulated with 5 μg/mL CTH522 or 1 μg/mL phytohemagglutinin (PHA; Roche, cat.: 11249738001) as positive control ...
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bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biophysics 2022Quote: ... 5 EDTA and 1% Triton X-100) supplemented with cOmplete™ Mini-EDTA free protease inhibitor cocktail (Roche, Germany). Lysates were incubated 5 min on ice and centrifuged 10 min - 14000 x g at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Developmental Biology 2023Quote: Lungs extracted from E14.5 or E18.5 embryos were minced and digested using collagenase I and dispase I (both 1 mg/mL, Roche) and incubated at 37°C for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... under gravity flow, washed with PER2 buffer (1 x TBS pH 7.5, 1mM EDTA, 5% glycerol, EDTA-free protease inhibitors (Roche)) and eluted with 3x flag peptide in PER2 buffer ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in 750 μl Tris lysis buffer (50 mM Tris, 0.2% NP-40, 150 mM NaCl, 5 mM EDTA, 5.7 mM PMSF, and 1 x Roche cOmplete). For whole organ samples (either whole detunicated testis or thymus) ...
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bioRxiv - Microbiology 2024Quote: ... each sample was normalized to 3.5 ng in 5 µL water before generating libraries using a 1:10 Kapa HyperPlus (Roche) miniaturized protocol with barcoded indices as previously described82 ...
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bioRxiv - Plant Biology 2024Quote: ... 50 mM Tris-HCl [pH 7.5], 150mM NaCl, 1 mM EDTA, 0.2% IGEPAL® CA-630, 0.5 mM DTT, 1X protease inhibitor cocktail [Roche]) in a 3:1 v/w ratio ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated in blocking solution containing 5% horse serum and 1% Western Blocking Reagent (Roche Diagnostics, Mannheim, Germany) in TNTx for at least 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2) supplemented with a cocktail of protease inhibitors (CLAPA and 1 µg/mL cOmplete, EDTA-free, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM pyridoxal 5’-phosphate hydrate (PLP) and proteases inhibitor cocktail (cOmplete™, EDTA-free Protease Inhibitor Cocktail Roche).
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bioRxiv - Immunology 2024Quote: ... Brain stems were cut into pieces and digested for 40 min at 37 °C with 5% CO2 in PBS supplemented with 1.6 IU ml-1 Collagenase/Dispase (Roche) and 0.5 mg ml-1 DNase I (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was resuspended in lysis buffer (80 mL per L of initial culture: 20 mM HEPES pH 8, 500 mM NaCl, 5% v/v glycerol, 0.5 mM TCEP + 1 Roche cOmplete EDTA-free protease inhibitor tablet ...
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bioRxiv - Cell Biology 2024Quote: ... and thawing and by resuspension in 5 mL of ice-cold Lysis/Wash buffer (25 mM HEPES pH 7.5, 1 M NaCl, 5% glycerol) supplemented with cOmplete protease inhibitor cocktail (Roche), followed by vigorous sonication on ice delivered by a Jencons Ultrasonic Processor (15s bursts at 70% amplitude) ...
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bioRxiv - Genetics 2024Quote: ... 1 mM DTT, 10 mM NaPPi, 5 mM EGTA, 5 mM EDTA, 0.1 mM Na3VO4, 5 mM NaF, and Roche cOmplete™ Protease Inhibitor Mix). Crude extracts were prepared by centrifugation ...
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bioRxiv - Immunology 2022Quote: ... were coated O/N at 4 °C with 50 μl of fibronectin (50 μg/ml; Roche) or VCAM-1-Fc (10 μg/ml R&D System) ...
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bioRxiv - Microbiology 2024Quote: ... The recognized protein bands were visualized by 3,3 N-Diaminobenzidine tertrahydrochloride (DAB) solution staining (Roche, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... before being spotted in 2.5 mcl drops onto a dry nylon membrane (Hybond-N+, Roche 11417240001). Biotinylated oligo-dT (Promega Z5261 ...
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bioRxiv - Cell Biology 2024Quote: ... 10ng of total RNA were processed using the KAPA mRNA HyperPrep Kit (Roche p/n KK8580) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche), as described previously (Kemaladewi et al. ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP) and cOmplete protease inhibitors (Roche). Cells were lysed by sonication and cell debris pelleted at 25,000 rpm for 40 min ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM Mg(OAc)2 supplemented with phosphatase and protease inhibitors (Roche) (adapted from (29)) ...
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bioRxiv - Plant Biology 2024Quote: ... 15 mM MgCl2, 1 mM EDTA, 10 % glycerol, 1 % Triton X-100, 10 mM β-mercaptoethanol, 2 mM PMSF and Roche cOmplete Protease Inhibitor Cocktail)) were added in the ground leaves and incubated for 30 min at 4 °C ...