Labshake search
Citations for Roche :
701 - 750 of 1169 citations for 192 IgG Mouse Monoclonal Cy3 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10) was labeled with digoxigenin-11-dUTP using DIG-Nick Translation Mix (Roche) or with biotin-16-dUTP (Promo Kine ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cDNAs were labeled with [α32P]-dCTP using the Random Primed DNA Labeling Kit following the protocol provided by Roche. Total RNA was TRizol (Invitrogen ...
-
bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin (DIG)-labeled sense and antisense riboprobes were prepared using the in vitro transcription DIG labeling kit (Roche, IN, USA). Hybridization was carried out at 600C overnight and the slides were washed and probed with an alkaline phosphatase-conjugated anti-DIG antibody (Roche ...
-
bioRxiv - Developmental Biology 2023Quote: ... Larvae were hybridized with digoxigenin (DIG)-labeled riboprobes overnight at 65°C and incubated overnight with 1/2000 alkaline phosphatase-conjugated anti-DIG Fab fragment (Roche) at 4°C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Slides were incubated with 50 μL of block buffer containing a fluorescein-labeled anti-DIG antibody (sheep, 1:100, Roche) for 60 min at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... A dioxigenin (DIG)-11-UTP-labeled antisense riboprobe targeting the gene transcript of interest was synthesized using the DIG labelling kit (Roche) and T7 RNA polymerase80 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Telomere Restriction Fragments (TRF) location on the blots was compared to the DNA molecular weight marker VII DIG-labeled (Roche) to obtain the average of TRF.
-
bioRxiv - Plant Biology 2023Quote: ... The 18S-1 probe contains a 552-bp fragment of the sugar beet 18S-5.8S-25S ribosomal RNA (rRNA) gene with the 35S rDNA (Schmidt et al. 1994) labeled with digoxigenin-11-dUTP by PCR (Roche) and the probe pXV1 for the 5S rRNA gene (Schmidt et al ...
-
bioRxiv - Plant Biology 2023Quote: ... and the probe pXV1 for the 5S rRNA gene (Schmidt et al. 1994) labeled with biotin-11-dUTP by PCR (Roche). The telomeric probe pLT11 from Arabidopsis (Richards & Ausubel 1988 ...
-
bioRxiv - Plant Biology 2023Quote: ... Digoxigenin-labeled RNA probes based on the sequence of VRN1 were synthesized using a DIG northern Starter Kit (Roche, 11277073910), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Digoxigenin (DIG)-labeled sense and antisense probes were generated with T7 and Sp6 RNA polymerase using a DIG RNA labeling kit (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... Paromomycin probe was labeled with dUTP-DIG during PCR reaction (PCR DIG probe synthesis kit, Roche; see table primers list). Membrane was pre-hybridized 1h at 50°C in a DIG eazy Hyb (Roche ...
-
bioRxiv - Molecular Biology 2023Quote: ... Prehybridization for 60 min and overnight hybridization were carried out with a DIG-labeled probe (1 µg) at 65 °C with DIG Easy Hyb Granules (Roche). A chemiluminescent assay was used to visualize the probe on the membrane ...
-
bioRxiv - Molecular Biology 2024Quote: ... The digoxigenin (DIG)-labeled probes were generated by PCR on H26 genomic DNA using primers and the PCR DIG labeling Mix from Roche. Probe hybridization was detected using the DIG Luminescent Detection kit (Roche ...
-
bioRxiv - Microbiology 2020Quote: ... permeabilized and immunostained with the following primary antibodies - rat monoclonal anti-HA (clone 3F10, Roche: 1:250 dilution), mouse monoclonal anti-myc (clone 9B11 ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were incubated in PEMBALG containing monoclonal anti-HA antibody produced in rat (1:1000 dilution, Roche) for one hour ...
-
bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h with rat anti-HA high affinity monoclonal antibodies (Roche Applied Science, Penzberg, Germany) at 1:500 ...
-
bioRxiv - Cell Biology 2022Quote: ... the whole-cell extracts and immunoprecipitates were subjected to western blotting using monoclonal anti-GFP (JL-8, Roche), Flag-M2 monoclonal antibody (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2023Quote: ... HA-and GFP-tagged proteins were detected using horseradish peroxidase–conjugated monoclonal anti-HA (Roche, 3F10, 1:5,000) and anti-GFP (Roche ...
-
bioRxiv - Cell Biology 2021Quote: ... A silanized grid was incubated in a drop of anti-DIG IgG (0.2µM, Roche), washed with MRB80 ...
-
bioRxiv - Cell Biology 2023Quote: ... The slides were blocked by incubation with Discovery goat IgG block (Roche; 760-6008) for 12 mins at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... Secreted IgGs were purified from cell supernatants with Protein A resin (Roche, Basel, Switzerland). IgGs were eluted at low pH using Protein A elution buffer (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... Colorimetric in situ hybridization assays used sense and anti-sense RNA probes labeled with DIG RNA labeling mix (Roche, Basel, Switzerland), following a published procedure (Sharma et al. ...
-
bioRxiv - Genomics 2019Quote: ... Supplementary Table S3) including 18S-28S rDNA and 5S rDNA probes were labeled with biotin-16-dUTP (Biotin Nick Translation Mix, Roche, USA) and/or digoxigenin-11-dUTP (Dig Nick Translation Mix ...
-
bioRxiv - Plant Biology 2020Quote: ... The sites of hybridization of digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science, Penzberg, Germany) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... The rDNA sequences were then labeled with Digoxigenin-11-dUTP by nick translation according to the manufacturer’s protocol (Roche, Ref 11277065910). For the detection of major satellites ...
-
bioRxiv - Molecular Biology 2022Quote: ... Biotin-labeled LPA1-HA was detected using streptavidin beads (Pierce) and conjugated anti-HA antibody (3F10 from Roche Diagnostics; 1:1000).
-
bioRxiv - Developmental Biology 2019Quote: CISH riboprobes were generated from the gDNA or cDNA with PCR and labeled with digoxigenin (DIG) using DIG RNA labeling mix (Roche, 11277073910) by in vitro transcription ...
-
bioRxiv - Plant Biology 2019Quote: ... were used as templates to synthesis DIG-labeled probes according to the manufacturer’s protocol (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche, Basel, Switzerland). ULTRAhyb®-Oligo Hybridization Buffer (Ambion ...
-
bioRxiv - Microbiology 2020Quote: ... were amplified using primers Sb.1 - Sb.2 and Sb.3 to Sb.8 (Table 2) and labeled with digoxigenin (DIG) using the PCR DIG probe synthesis kit (Roche Diagnostics). Membranes were hybridized with these probes at 65°C and then washed at 68°C with decreasing concentrations (from 2X to 0.2X ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then hybridized overnight at 70 °C with a digoxigenin (DIG)-labeled RNA probes (DIG RNA labeling kit, Roche 11277073910) for Gadd45b mRNA ...
-
bioRxiv - Molecular Biology 2020Quote: ... A particular tRNA was hybridized with a corresponding antisense oligo DNA probe shown in Table S1 labeled with digoxigenin (DIG) using DIG Oligonucleotide Tailing Kit (Roche Diagnostics) in Hybridization Solution [0.50 M Na2HPO4 ...
-
bioRxiv - Molecular Biology 2022Quote: CISH riboprobes for Spp1 and Myh9 were generated from gDNA or cDNA with PCR and labeled with digoxigenin (DIG) using DIG RNA labeling mix (Roche, 11277073910) by in vitro transcription ...
-
bioRxiv - Genomics 2023Quote: ... was indirectly labeled by PCR in the presence of digoxigenin-11-dUTP detected by antidigoxigenin-fluorescein isothiocyanate (FITC; both from Roche Diagnostics). The remaining satDNAs (‘pTS5’ ...
-
bioRxiv - Immunology 2023Quote: ... rs9500880 and rs1921622 was conducted by PCR utilizing fluorescent-labeled hybridization FRET probes followed by melting curve analysis in a LightCycler 480 (Roche Diagnostics). The following probes and primers were used ...
-
bioRxiv - Zoology 2023Quote: The PCR products amplified from both IHHNV-309 and IHHNV-389 methods were used as templates to prepare digoxigenin (DIG)-labeled IHHNV probes using a PCR DIG-labeling kit (Roche, Germany). After preparation ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
-
bioRxiv - Molecular Biology 2023Quote: ... and fluorescein (FITC)-labeled riboprobes were synthesized using T7 or SP6 polymerase along with a DIG or FITC labeling mix (Roche Diagnostics). For the detection of hybridization signals ...
-
bioRxiv - Genomics 2024Quote: ... and 22-bp Sat III were synthesized as probes and labeled with Dig-dUTP or Bio-dUTP (Roche Diagnostics, Basel, Switzerland) using the Biotin and Dig Nick Translation Mix (Roche ...
-
bioRxiv - Physiology 2024Quote: ... Fluorescein-labeled cRNA probes for esr1 and esr2a were generated by in vitro transcription using Fluorescein RNA Labeling Mix (Roche Diagnostics), SP6 or T7 RNA polymerase (Roche Diagnostics) ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-GFP (Roche, # 11814460001, 1/1000), rabbit anti-calreticulin (Abcam ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-GFP (1:1000, Roche 11814460001), goat anti-SYP-1 (1:500 ...
-
bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GFP (7.1+13.1, Roche, 11814460001) and normal rabbit IgG (Millipore 12-370).
-
bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated anti-mouse secondary antibody (Roche) and ECL (PIERCE ...
-
bioRxiv - Cell Biology 2019Quote: ... anti-myc from mouse (9E10; 11667149001; Roche) and anti-HA from mouse (12CA5 ...
-
bioRxiv - Microbiology 2019Quote: ... anti-GFP mouse 1:100 (11814460001; Roche), anti-HA rabbit 1:50 (H6908 ...
-
bioRxiv - Microbiology 2019Quote: ... mouse anti-GFP (1:1000, Roche Diagnostics) and rabbit anti-mCherry (1:1000 ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse anti-GFP (11814460001; Roche, 1:200). Slides are coated with 100 µl of primary antibody for 1 hour followed by washing with PBS three times with 5 min incubation each time ...
-
bioRxiv - Genetics 2021Quote: Mouse bi-clonal anti-GFP antibody (Roche Diagnostics ...