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Citations for Roche :
7351 - 7400 of 8559 citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... comprising 0.5 μL of DNA (40 ng/μL) and 1 μL of LightCycler 480 SYBR Green I Master mix (Roche) containing 0.5 μM PCR primers (Eurogenetec SA) ...
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bioRxiv - Molecular Biology 2021Quote: ... in a total volume of 40 µl to 2.4 pmole trypSL in the presence of 1 mM ATP and 20 U RNasin (Roche) in vaccinia capping buffer ...
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bioRxiv - Molecular Biology 2021Quote: In situ antibody stainings were done as described previously 31 using rat anti-HA (MAb 3F10, 1:20; Roche), rabbit anti-FLAG (M2 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4°C with an established dilution in fresh-prepared sample buffer (1% bovine serum albumin and 0.05% Tween-20 in PBS) supplemented with protease inhibitor (cOmplete, Roche). Detection antibody (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was resuspended in 5ml lysis buffer (20 mM Tris, 150 mM NaCl, 1% NP40 pH 7.5, plus a tablet of protease inhibitor cocktail from Roche) per mg of cell pellet and incubated at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: Mouse livers were homogenized with a motorized tissue grinder in STM buffer (Tris-HCl pH 7.4, 5 mM MgCl2, 250 mM Sucrose, 1 mM DTT, supplemented with 500 µM PMSF, Protease Inhibitor Cocktail [Roche, Basel ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in 500 μl lysis buffer (50mMTris-HCl, pH8.0, 100mM NaCl, 10m MEDTA, 5% glycerine, 1% NP- 40 and protease inhibitor cocktail (Roche)) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), washed once with PBS ...
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bioRxiv - Genomics 2020Quote: ... and mixed with 100 μL Binding Buffer (1% Triton X-100, 0.1% Sodium Deoxycholate, 1x complete protease inhibitor (Roche)) plus 100 μL 0.2 μg/μl chromatin followed by overnight incubation on a rotating platform at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Between 60-80 mg pellets were lysed in 1mL lysis buffer (50mMTris-Cl pH 7.0, 10mM EDTA, 1%SDS, 1mM PMSF, complete protease inhibitor cocktail (Roche), 0.1U/mL RiboLock RNase inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... 1mM EGTA, 1mM EDTA, 1% Triton X-100, 0,27M sucrose, 1mM DTT, cOmplete EDTA-free protease inhibitor cocktail – Roche, 1mM NaF ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were incubated with 50 μl of 1:10 Enzyme:TUNEL reagent (TdT and fluorescein-dUTP) (Roche, Cat No. 11684795910) at 37°C for 3 h in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... or NETN Buffer (20 mM TRIS pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% IGEPAL, 1.25 mM DTT and Roche Protease Inhibitor) with several freeze/thaw cycles ...
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bioRxiv - Cancer Biology 2019Quote: ... the cell pellets were resuspended in lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 % NP-40, 5 mM MgCl2, 5 % glycerol and protease inhibitor cocktail [Roche]) and incubated for 20 min at 4 °C with end-over-end rotation ...
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bioRxiv - Cell Biology 2019Quote: ... 100 mM KOAc pH 7.5, 10 mM MgSO4, 10 µM ZnSO4, 1 mM PMSF and complete protease inhibitors from Roche), and disrupted by bead beating with a FastPrep 24 (MP Biomedical ...
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bioRxiv - Neuroscience 2019Quote: ... The brains were transferred to an 1.5 ml Eppendorf tube containing 500 microliters 1 mg/ml Liberase DH (Roche, prepared according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2019Quote: ... Immunoprecipitated proteins were immunoblotted for GFP- or HA-epitope tagged ubiquitin with antibodies for GFP (1:3,000, Roche #11814460001) or HA (1:4,000 ...
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bioRxiv - Immunology 2019Quote: ... containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Immunoblots were carried out using the following antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were rinsed twice with PBS and resuspended in 1 mL lysis buffer (1X PBS, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor cocktail (Roche, #11836170001). Chromatin was sheared to 200-500 base pairs using a Covaris E220 sonicator and cleared by centrifugation for 15 minutes at 19,000 × g ...
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bioRxiv - Physiology 2019Quote: ... Tissue was placed in ice-cold hypotonic lysis medium (HLM, 10 mM Tris, pH 7.4, 1 mM EDTA) in the presence of a protease inhibitor cocktail (Roche), minced and homogenized by 20 strokes in a glass homogenizer ...
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bioRxiv - Genetics 2019Quote: ... DNA was eluted in 100μl of TE + 1%SDS and the beads were washed and eluted a second time with 100μl of TE + 1%SDS + 5 μl of 20mg/mL Proteinase K (Roche). Following overnight incubation at 65°C ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed in ice-cold PBS and lysed in lysis buffer (100 mM TrisHCl pH 7.5, 150 mM NaCl, 1% Triton X-100, protease inhibitors (Roche)) for 30 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 500 mM NaCl, 10% glycerol, 1 mM DTT and complete EDTA-free protease inhibitors cocktail [Roche]) supplemented with 0.02% NP40 and 1 mM PMSF ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were lysed in 5 pellet volumes of RIPA buffer (25 mM Tris, 150 mM NaCl, 1 % Triton X-100, 0.5 % Sodium deoxycholate, 0.1 % SDS, pH 7.5, 1x Roche Protease Inhibitors) supplemented with 50 units BaseMuncher (Expedeon ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then vigorously resuspended in 1ml of fresh ice-cold lysis buffer (50mM Tris pH 8, 150mM NaCl, 0.5% NP-40, 1 tablet of complete protease inhibitor (04693159001, Roche)) and transferred to 9 ml of pre-chilled lysis buffer and incubated for 20 min on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... Every 10 million cells were lysed by 300μL 1% SDS include freshly made Protease Inhibitor (PI) cocktail (Roche #4693132001), sonicated with Diagenode Bioruptor Pico and diluted with 2.7mL PBA (1x PBS + 0.5% BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.15 μM each of PaaDlp-2_R1 and R2 in 1 × KAPA HiFi HotStart ReadyMix (KAPA Biosystems, Wilmington, WA, USA) and a 2-μL sample of eDNA ...
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bioRxiv - Microbiology 2019Quote: ... The solution was then supplemented with 20 mM MgSO4 and treated with 50 μg mL-1 RNase A (Roche) and 10 μg mL-1 DNase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... before lysing in lysis buffer ((1% Triton X100, 50 mM HEPES, 150 mM KCl, 1 mM MgCl2, pH 7.4, with 1x EDTA-free protease inhibitors, Roche) at 4 °C for 2 hrs ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... The samples were incubated on ice for 10min and then lysates were diluted 1:2 with CNBr IP wash buffer (50 mM Tris–HCl pH 7.5, 150mM NaCl, 1mM EDTA, 1/50 protease inhibitor tablets [Roche]). The homogenates were incubated on ice for 15min and were cleared by centrifugation at 12,000g for 15min at 4°C ...
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bioRxiv - Genetics 2021Quote: Neuronal cultures were lysed in 20 µl of NP40/SDS buffer (1% Nonidet P40/0.01% SDS lysis buffer and protease inhibitors (Roche) per well ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for one hour in donkey anti-mouse FITC antibody (1:200) in 0.3% Triton-X100 supplemented with 1X blocking buffer (Roche). After washing with PBS for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
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bioRxiv - Genetics 2020Quote: ... Cells pellets were combined and lysed in a denaturing urea buffer (8 M urea, 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml ...
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bioRxiv - Cell Biology 2021Quote: ... A total of ∼ 8×107 cells were lysed in 2 mL lysis buffer (20 mM Tris HCl pH 7.4, 150 mM NaCl, 1% Triton-X 100 supplemented with protease inhibitor Complete, Roche). Lysates were rotated at 4°C for 20 minutes and subsequently centrifuged at maximum speed for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Activation of PI3K and MAP kinase signaling was assessed by immunoblot for pAkt (Phospho-Akt (Ser473 ...
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bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
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bioRxiv - Plant Biology 2020Quote: ... 150 mM NaCl, 5 mM EDTA, 5 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Neuroscience 2020Quote: ... Each tissue sample was dissociated using 2.4 mL of homogenization buffer (10 nM Tris pH 8, 5 mM MgCl2, 25 mM KCl, 250 mM sucrose, 1 μM DTT, 0.5x protease inhibitor [cOmplete, Roche #4693159001] ...
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bioRxiv - Cancer Biology 2021Quote: GSDMB2-HA expression was analyzed by immunohistochemistry in 5µm-thick tissue sections using rat anti-HA (1:200; 3F10, ROCHE) or mouse monoclonal anti-GSDMB (1:10 ...
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bioRxiv - Biochemistry 2020Quote: ... The medium was discarded and the cells were resuspended in 200 µL of ice-cold 50 mM HEPES buffer pH 7.4 containing 1 x protease inhibitor cocktail (Roche) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested 4 hours after induction at 37°C and lysed by sonication in 1x PBS pH 7.5 with 1 tablet protease inhibitor cocktail (Roche). After cell lysis and centrifugation at 30,000g ...
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bioRxiv - Neuroscience 2021Quote: ... 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor; Roche) and filtered through 40 μm filter (BD falcon ...
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bioRxiv - Neuroscience 2020Quote: ... The ipsi- and contralateral cortices were immediately transferred to lysis buffer (50 mM Tris, pH 7.6, 150 mM NaCl, 1% Triton X-100, CompleteMini Protease Inhibitor Mixture, Roche). The cortices were homogenized and incubated on ice for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with ice-cold 1x PBS added with 1.5 mM Na3VO4 and 50mM NaF before being lysed in lysis buffer (1X PBS, 1% Tx-100, 1.5 mM Na3VO4, 50mM NaF, PhosSTOP (Roche) and cOmplete protease inhibitors (Roche)) ...
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bioRxiv - Biochemistry 2021Quote: ... Mitochondria were resuspended in SEM buffer (250 mM sucrose, 1 mM EDTA, 10 mM MOPS-KOH, pH 7.2, containing 1X protease inhibitor cocktail from Roche).
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bioRxiv - Cell Biology 2021Quote: ... in lysis buffer (150 mM NaCl, 1% NP-40, 50mM Tris pH 8.0, 1x Roche protease inhibitor tablet (11697498001)) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with ice-cold 1x PBS added with 1.5 mM Na3VO4 and 50mM NaF before being lysed in lysis buffer (1X PBS, 1% Tx-100, 1.5 mM Na3VO4, 50mM NaF, PhosSTOP (Roche) and cOmplete protease inhibitors (Roche)) ...