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Citations for Roche :
7001 - 7050 of 7615 citations for 7 Methylimidazo 1 2 a pyridine 3 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of the reverse transcrip-tion mix from the Titan One Tube RT-PCR System kit (Roche, Switzerland) was added ...
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bioRxiv - Cell Biology 2024Quote: ... All lysis buffers were supplemented with phosphatase inhibitors NaF (10 mM) and Na3VO4 (1 mM) along with 1X protease inhibitor cocktail (ROCHE cOmplete Protease Inhibitor Cocktail Cat # 1169498001 ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using a 1% triton X-100 containing lysis buffer (CST) supplemented with protease inhibitor cocktail (Roche) on ice for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Prehybridization for 60 min and overnight hybridization were carried out with a DIG-labeled probe (1 µg) at 65 °C with DIG Easy Hyb Granules (Roche). A chemiluminescent assay was used to visualize the probe on the membrane ...
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bioRxiv - Pathology 2024Quote: ... cDNA product was 1 to 40 diluted and real-time PCR was performed using FastStart SYBR Green Master Mix (Roche) on QuantStudio 5 (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... The gDNA levels corresponding to the viral genes and the housekeeping wasp gene (elongation factor (ELF-1)) were determined using the LightCycler 480 System (Roche). The cycling conditions involved heating at 95°C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl of IPed proteins and 10 µl of WCE were submitted to SDS-PAGE and analyzed by immunoblotting using anti-HA antibody (1:250, ref.12013819001, Roche), anti-Flag antibody (1:500 ...
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bioRxiv - Microbiology 2024Quote: HeLa cells treated with or without CsA were infected with HIV-1 (10 ng p24) that had been treated with 100 U/ml of DNase I (Roche) for 1 h at 37° C ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were harvested and stored at -80°C in ICD lysis buffer (300 mM NaCl, 50 mM Tris/HCl, 1 mM EDTA, 1x Protease Inhibitor (Roche), pH 7.4) ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked in 5% non-fat milk in TBST for 1 h and immunoblotted with an antibody against HA directly conjugated to HRP (anti-HA-HRP; Roche) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Different dilutions of the enzyme preparation were assayed for 1 hour and analyzed with a luciferin-luciferase coupled reaction (76) using the CLSII-Bioluminiscent ATP Assay Kit (Roche).
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bioRxiv - Molecular Biology 2024Quote: ... whole cell lysate samples were prepared by resuspension and trituration of cell pellets in a lysis buffer (20 µM Tris-HCl pH 7.4, 1 µg/mL DNaseI, 1X cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Developmental Biology 2024Quote: ... Phosphorylated and annealed oligos were diluted 1:10 and a Golden Gate reaction was setup with 1x rapid ligase (Roche), 10 units of BbsI enzyme ...
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bioRxiv - Microbiology 2023Quote: Cells were washed in PBS and harvested in lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris-HCl, protease inhibitor (Roche complete mini EDTA-free protease inhibitor) ...
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bioRxiv - Plant Biology 2023Quote: ... Detection of alkaline phosphatase was carried out for ∼1 h using NBT/BCIP ready-to-use stock solution (La Roche) diluted in 1× washing buffer.
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bioRxiv - Neuroscience 2023Quote: ... the supernatant replaced by 1ml PBS-Glucose supplemented with 50 µl Collagenase/Dispase (Worthington CLS-1 345U/mg; Dispase II Roche 1.2U/mg ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing 40mg/L hygromycin (Roche, no. 10843555001). For each construct ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA (1 μg) was reverse transcribed to cDNA using the Transcriptor First Strand cDNA Synthesis Kit (catalog no. 04379012001, Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were permeabilised by 10 minutes incubation with 0.1% Triton X-100 on ice and incubated with blocking buffer (10% FBS in 1X PBS) for 90 minutes and then probed with the primary antibody (anti-HA rat 1:100 (Roche) for 2 hours on rotor and after three washing with 1x PBS cells were probed with anti-rat Alex Flour-488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: Frozen liver tissues that were crushed using a Multi-beads Shocker (Yasui Kikai, Japan) or cultured cells were dissolved in RIPA buffer containing 1× cOmplete proteinase Inhibitor Cocktail (Roche). Protein concentration in the lysates was determined using a BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The final pellet was resuspended in 750 uL nuclear lysis buffer (10 mM EDTA, 1% SDS, 50 mM Tris-HCl at pH 8, 1x cOmplete protease inhibitor cocktails (Roche)) ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MAB-Buffer2 (20% sheep serum, MAB-Buffer1) for 1h and incubated with anti-DIG-AP antibody (1:4000, Roche) in MAB-Buffer2 overnight at 4 °C.
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bioRxiv - Genomics 2023Quote: ... and collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor [#11873580001, Roche] in PBS). Chromatin was sonicated with a Covaris E220 sonicator (140PIP ...
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bioRxiv - Immunology 2024Quote: ... Lungs were resected and minced with scissors before incubation in a collagenase buffer containing 1 mg/mL Collagenase-A (Roche), 2000U DNaseI (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed with sonication in lysis buffer (50mM Hepes, 500mM NaCl, 1% Tween20, 5% Glycerol, half a tablet of cOmplete EDTA-free protease inhibitor cocktail [Roche]). The suspension was sonicated at 150J in intervals of 1.5secs for 20 sec total in ice ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested after 48 hours and lysed in 50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100, protease inhibitor (cOmplete™, Mini, EDTA-free (Roche)) ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were transferred to a PVDF membrane and immunodetected with an anti-Myc-HRP antibody (Myc-HRP, 1:5000, Roche). The remaining 10% of the immunoprecipitated samples together with 50 μg of the input were subjected to the same procedure but probed with an anti-GFP antibody (JL-8 ...
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bioRxiv - Bioengineering 2024Quote: cDNA from cells was diluted 1:10 and qPCR was performed in triplicate using LightCycler 480 SYBR Green I Mastermix (Roche). Samples containing primers specific for lmna ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were disrupted in 1% NP-40 lysis buffer (140 mM NaCl, 10 mM Tris-HCl pH 8, 1% NP-40) supplemented with proteinase inhibitors (Roche). Proteins were separated by electrophoresis (SDS-PAGE) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membrane lysis was performed in 5Lml LB1 (50LmM HEPES pH 7.5, 140LmM NaCl, 1LmM EDTA, 10% glycerol, 0.5% 1% IPEGAL-CA630, 0.25% Triton X-100, and Roche protease inhibitors 11836170001) for 10 min at 4 °C with rotation ...
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bioRxiv - Immunology 2023Quote: ... The cell pellets were treated with lysis buffer (50 µg/mL digitonin, 75 mM NaCl, 1 mM NaH2PO4, 8 mM Na2HPO4, 250 mM sucrose, and Roche cOmpleteTM protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked for 30 min and then incubated in an alkaline phosphatase conjugated anti-DIG antibody (1:600, Roche). Slides were then washed and developed overnight in BCIP/NBT chromogen (Perkin Elmer) ...
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bioRxiv - Neuroscience 2023Quote: ... then lysed on ice for 15 minutes in non-denaturing lysis buffer (150 mM KCl, 10 mM MgCl2, 5mM HEPES, 1% IGEPAL) supplemented with protease inhibitors (Roche Complete EDTA-free ...
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bioRxiv - Bioengineering 2024Quote: ... livers were perfused at low flow for about 10 min with digestion buffer (low-glucose DMEM supplemented with 1 mM Hepes) containing freshly added Liberase (32 μg/ml; Roche). Digestion was stopped using isolation buffer (low-glucose DMEM supplemented with 10% FBS) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 mM MgCl2 and 1 mM DTT supplemented with DNase I (10 µg/ml) and complete protease inhibitor cocktail tablets (Roche). Complex formation was initiated by adding synthesized Stachel peptide (100 µM ...
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bioRxiv - Pathology 2023Quote: ... Spain) (1:10000) for 1.5h in darkness was followed by the addition of HRP substrate (11112422001, Roche Applied Science, Germany). Absorbance was measured using NanoQuant Infinite M200 Pro multi-plate reader (Tecan ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in PBS for 30 min and incubated overnight at 4°C with 1:1,500 anti-HA high affinity (Roche, 3F10). After three PBS washes ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR was set up as a 10μL reaction using SYBR® Green 1 480 Lightcycler® Master (Roche) in a 384-well plate ...
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: HK cells were lysed using a lysis buffer (50mM Hepes, 130mM NaCl, 1mM DTT 1% NP-40) with complete protease inhibitor cocktail (Roche). Cell lysates were centrifuged at 13,000 rpm for 10 mins at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant was centrifuged at 20000 g for 30 min at 4°C and the resulting pellet was solubilized with lysis buffer (20 mM Tris–HCl pH 8, 150 mM NaCl, 1% TritonX100, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then subjected to a transcardial perfusion with calcium-free Tyrode’s solution containing protein phosphatase-inhibitors (+PPIs) (1 tablet PhosSTOP per 10 ml) (Roche 04906845001) followed by Zamboni’s fixative containing 4% paraformaldehyde and 0.2% picric acid in 0.1 M phosphate buffer pH 7.4 +PPIs ...
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bioRxiv - Microbiology 2022Quote: Lungs were perfused with sterile PBS and digested at 37°C for 1 h with 625 μg/mL collagenase D (Roche) and 75 U/mL DNase I (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with ice-cold PBS and scraped into lysis buffer (1 mM EPPS, 8 M urea, protease (complete mini, EDTA-free) inhibitors (Roche) and PhosSTOP phosphatase inhibitor (Roche)) ...
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bioRxiv - Neuroscience 2023Quote: ... Five P2 mouse brains were homogenized in 5 mL of homogenization buffer (5 mM Tris-HCl, 0.32 M sucrose, 1 mM MgCl2) supplemented with EDTA-free protease inhibitors (cOmplete™, Roche), then centrifuged at 1,660 x g for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Whole kidneys were homogenized and protein was extracted from 20 mg of tissue in 1 ml RIPA buffer supplemented with protease inhibitors (Roche) for 2 hrs ...
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bioRxiv - Cancer Biology 2022Quote: Frozen bacteria pellets were thawed on ice and loosened in 25 mL lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X100, 10 mM DTT, 1X Protease Inhibitor Cocktail (Roche)) ...