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Citations for Roche :
651 - 700 of 2228 citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and goat anti-human AF647 (Stratech 109-606-088- JIR)) DNA was then stained with DAPI (Roche) and coverslips mounted with Vectashield (Vector Laboratories - Vector H-1000).
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bioRxiv - Immunology 2024Quote: Mice or Human CD4 T cells were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Pierce) ...
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bioRxiv - Biochemistry 2021Quote: Cardiac myofibrils were prepared from left ventricular trabecular strips pre-stretched overnight to a sarcomere length of about 2 µm in rigor buffer (20 mM HEPES pH 7, 140 mM KCl, 2 mM MgCl2, 2 mM EGTA, 1 mM DTT, Roche complete protease inhibitor) at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Samples were ground to powder in liquid nitrogen and then dissolved in 2 mL lysis buffer (8 M urea, 2% SDS, 1x Protease Inhibitor Cocktail (Roche Ltd. Basel, Switzerland), followed by sonication on ice for 30 min and centrifugation at 13 000 rpm for 30 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Reverse crosslinking was performed overnight at 65>°C with high NaCl and RNase treatment (Roche). Proteinase K (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The Micro-C library was generated by using the KAPA HiFi HotStart ReadyMix (Roche #KK2601) or the Q5 High-Fidelity 2X Master Mix (NEB #E7645 ...
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bioRxiv - Genomics 2019Quote: All buffers were pre-chilled to 4°C with cOmplete EDTA-free protease inhibitor (Roche) freshly added ...
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bioRxiv - Evolutionary Biology 2019Quote: ... animals were incubated overnight with peroxidase-conjugated antibodies at 4°C (anti-DIG-POD, Roche 1:500 dilution and anti-DNP ...
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bioRxiv - Systems Biology 2021Quote: ... epidermal sheets were digested in Liberase Tm (13 U/ml, Roche, UK, 2h, +37°C,) and enriched using density gradient centrifugation (Optiprep 1:4.2 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated overnight at 4 °C with rat anti-HA antibody (1:500 - Roche) and then with secondary goat anti-rat IgG antibody coupled to Alexa 568 (Invitrogen/Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 10 min at 37°C with anti-HA antibody (3F10; Roche) (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... minced and enzymatically dissociated at 37°C for 45 min in 0.2% collagenase A (Roche) and 1 mg/ml dispase (GibcoBRL) ...
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bioRxiv - Molecular Biology 2020Quote: HDL and LDL measurements were done using the Cobas C-111 chemistry analyzer (Roche, Switzerland).
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bioRxiv - Genomics 2021Quote: ... The membrane was hybridised overnight at 42 °C with DIG Easy Hyb (Roche®, 11603558001). The DNA was detected with anti-digoxigenin-Alkaline Phosphatase antibody (Roche® ...
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bioRxiv - Molecular Biology 2022Quote: ... 72 °C for 10 s) using KAPA Hifi Hot Start Ready Mix (KAPA Biosystems KK2602) and primers (fw ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed at 4°C in RIPA buffer supplemented with protease inhibitors (Roche, 4693159001). Protein concentrations were measured using a BCA Assay Kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: ... S195A-GD-FXa protein was further purified on an anti-Protein C Affinity matrix (Roche). Maldi mass spectrometry showed that TFPI was glycosylated ...
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bioRxiv - Developmental Biology 2022Quote: ... cv-c riboprobe was marked using DIG RNA Labelling Kit (Roche, 11 175 025 910). Images were taken on an SPE Leica confocal microscope and processed using FIJI and Adobe Photoshop programs.
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bioRxiv - Microbiology 2023Quote: ... and treated for 30 min at 37°C with 20 U/ml DNase I (Roche). Virus was pelleted through a 25% sucrose cushion ...
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bioRxiv - Developmental Biology 2023Quote: ... reverse crosslinked by incubation overnight at 65°C in the presence of proteinase K (Roche), and cleaned by RNase A (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... diluted in 100 mM ammonium acetate (pH = 4) and digested with Glu-C protease (Roche) at 10:1 protein:enzyme for 1 h at room temperature prior to mass spectrometric analysis ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was pre-hybridized and hybridized at 50°C in DIG eazy Hyb (Roche). Two double washing steps were realized using 0.1% SDS ...
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bioRxiv - Immunology 2023Quote: ... and digested for 1 h at 37°C in 94 µg/mL DNase I (Roche) and either 250 µg/mL Liberase DL (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were deparaffinized followed by antigen retrieval in CC1 buffer (pH 9, 95°C; Roche), endogenous peroxidase blocking ...
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bioRxiv - Developmental Biology 2023Quote: ... 1xSSC and 0.5xSSC at 55°C followed by anti-Digoxigenin antibody (Roche #11093274910, 1:500) incubation for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and digested for 1 hour at 37°C in 94 μg/mL DNase I (Roche) and 250 μg/mL collagenase type I and 250 μg/mL collagenase type IV (Worthington Biochemicals) ...
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bioRxiv - Neuroscience 2024Quote: ... incubated with anti-DIG-POD antibody (Roche; 1:1000 in TNB; 12 hr, 4 °C), and washed in TNT (3 × 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were incubated overnight at 4°C with Anti-Digoxigenin-AP antibody (1:2000, Roche) in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C in horseradish peroxidase-coupled anti-DIG antiserum (11207733910, Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Genomics 2021Quote: ... and then resuspended in Antibody buffer (10 mM HEPES pH 150 mM NaCl, 2 mM spermidine, 2 mM EDTA, 0.1% BSA, and Roche complete EDTA-free protease inhibitor) with primary anti-Pol2S5p antibody (Cell Signaling Technology cat ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... 2 mM MgCl2 and EDTA-free protease inhibitor cocktail (Roche)) and lysed in an ice-cold sonicating water bath for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... 25 μl of 2× HIFI HotStart Ready Mix (Kapa Biosystems), and 0.2 M SMART PCR Primer and polymerase chain reaction (PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... PMSF with 2 cOmplete protease inhibitor cocktail tablets (Roche 04693116001) per 50 mL of buffer) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg/ml RNase A and protease inhibitor mixture (Roche). Proteins were bound to GST resin (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg/ml Rnase A and protease inhibitor tablets (Roche)) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM TCEP and a cocktail of protease inhibitors (Roche) using a syringe fitted with a 26G ½ needle ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 2% FCS and 1mg/mL dispase II (Roche) overnight at 4°C ...
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bioRxiv - Systems Biology 2019Quote: ... 2% SDS and protease inhibitors (complete mini., EDTA-free; Roche). Cells were lysed by sonication ...
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bioRxiv - Genomics 2021Quote: ... 0.0075% DIG and 2 U/ul Protector RNase inhibitor (Roche)) for 1 min on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... The embryos were blocked with 2% Blocking Reagent (Roche, Mannheim) for 1h ...
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bioRxiv - Neuroscience 2020Quote: ... and 10 ng/mL fibroblast growth factor 2 (FGF2, Roche). Oligodendrocyte precursor cells were removed by brusquely shaking the culture flasks several times when changing the medium after 2 or 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100) with 2% complete protease inhibitor (Roche) for 15 min on ice ...
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bioRxiv - Neuroscience 2021Quote: ... and a phosphatase inhibitor cocktail (2×PhosSTOP from Roche Diagnostics). Proteins were quantified using the Bio-Rad DC Protein assay (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2% SDS) in the presence of cOmplete and PhosSTOP (Roche). Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% 2-mercaptoethanol) in the presence of protease inhibitors (Roche) and phosphatase inhibitors (1 mM sodium orthovanadate ...
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bioRxiv - Cell Biology 2021Quote: ... PMSF (2 mm) and Protease Inhibitor Tablet (Roche, Basel, Switzerland), RNase A (1 mg·mL−1 ...
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bioRxiv - Cell Biology 2021Quote: ... PMSF (2 mm) and Protease Inhibitor Tablet (Roche, Basel, Switzerland), RNase A (1 mg·mL−1 ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM EDTA pH 8.0) containing Protease Inhibitor Cocktail (Roche). Nuclei were lysed using a Misonix XL-2000 Probe Sonicator with 5-10 second ON / 20 second OFF intervals until the lysate was clear (2 rounds for P0 ...
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bioRxiv - Immunology 2022Quote: ... 2 mM EDTA and 10% glycerol with protease inhibitors (Roche) and phosphatase inhibitors (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... The lungs were inflated with dispase (2 U/mL; Roche) in Dulbecco’s modified Eagle’s medium (DMEM ...