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Citations for Roche :
651 - 700 of 10000+ citations for Human Complement Component 1 Q Subcomponent Binding Protein HABP1 C1QBP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: H9 human pluripotent stem cells were maintained in E8 media and passaged every four days onto matrigel-coated plates (Roche). ESCs ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
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bioRxiv - Immunology 2023Quote: K2 cells or human neutrophils were lysed with 150-200 µl of lysis buffer (supp. Table 2) supplemented with 1X complete inhibitor (Roche) and incubated for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Frozen brain tissues from human and animals were used to prepare 10% (w/v) homogenates in RIPA buffer containing PI and PhosStop (Roche). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... followed by library preparation with KAPA RNA HyperPrep kit (Roche, kit code KK8541), according to manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 1 mM sodium orthovanadate, 1 mM PMSF and Roche’s protease inhibitor tablet (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse transcribed using Transcriptor High Fidelity cDNA Synthesis Kit and a specific 3’-UTR DENV-1 primer (Roche Applied Science, Mannheim, Germany), d1a5B 5’-AGAACCTGTTGATTCAACRGC-3’ (62) ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted by incubation with RIPA buffer and protease inhibitor cocktail (Roche, 4693159001) on ice for 1 hour with occasional vortexing ...
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bioRxiv - Microbiology 2019Quote: ... GRA16HA (and other HA-tagged proteins) was detected using rat anti-HA antibodies (Roche) while GRA24MYC was detected using rabbit anti-MYC tag antibody 9E10 (Santa Cruz Biotechnology) ...
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bioRxiv - Biochemistry 2019Quote: ... washed and dried before digestion of proteins with 12.5 ng/μl of trypsin (Roche) in 25 mM NH4CO2 and 10 mM CaCl2 overnight at 25°C ...
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bioRxiv - Neuroscience 2019Quote: ... The protein lysates were extracted in presence of a protease cocktail tablet (Roche Diagnostics) using Cell Lysis buffer (ThermoFisher) ...
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was removed by the addition of 100 µl homogeneous Protein G-Agarose (Roche) and a further incubation for 3 h ...
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bioRxiv - Developmental Biology 2022Quote: ... The lysate was precleared by incubation with 50 μL protein-A agarose beads (Roche) for 1 h and then incubated with anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were generated using RIPA lysis buffer supplemented with protein inhibitor (Roche, #4693159001) and protein concentration was determined using Pierce™ BCA protein assay ...
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bioRxiv - Cancer Biology 2020Quote: ... Separated proteins were then transferred to a methanol-activated polyvinylidene difluoride membrane (PVDF, Roche) using wet transfer Mini-PROTEAN II electrophoresis system (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... GFP-tagged proteins were detected with a primary mouse antibody IgG1K Anti-GFP (Roche) diluted to 1:1000 in 5 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then treated with 8 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 3 hours followed by 19 μg/sample of trypsin (Roche Cat ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were then extracted in an IP buffer with a phosphatase inhibitor (Roche, PhosSTOP), centrifuged at 1700g for 5 min and filtered through miracloth ...
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bioRxiv - Cancer Biology 2019Quote: Protein was extracted from cultured cells in RIPA buffer containing a protease inhibitor (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: Total protein was collected in radioimmunoprecipitation buffer (RIPA) with PhosSTOP (Roche Diagnostics, Mannheim, Germany) and Complete Mini protease inhibitor cocktail tablets (EMD Chemicals Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... Following a 30 min preclear using blocked Protein A or G agarose beads (Roche), the chromatin was incubated with 5 µg antibody overnight at 4 °C (antibodies are listed in Table S6) ...
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bioRxiv - Cell Biology 2019Quote: ... Treated cells were lysed in protein lysis buffer with protease and phosphatase inhibitors (Roche). Selected cytokine targets were measured in cell culture supernatants and cell lysates with commercially available Luminex® Multiplex Assays (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol (TS130G) in the presence of cOmplete protein inhibitor cocktail (Roche). The resulting samples were clarified by centrifugation in an SS34 rotor (27,000 xg ...
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bioRxiv - Cell Biology 2020Quote: ... followed by addition of 5µl (packed volume) of protein G-coupled Agarose beads (ROCHE) and continued overnight incubation at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... the supernatant was cleared with 50µl of Protein agarose G (Roche cat no. 11719416001) for 3 hours at 4 °C and subsequently incubated with 50 µl of Protein agarose G with corresponding primary antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Phosphorylated proteins were visualized by chemiluminescence using Lumi-Light Western Blotting substrate (Roche, 12015196001) or SuperSignal West Femto Maximum Sensitivity substrate (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation (IP) of HA-tagged proteins was performed using Anti-HA Affinity matrix (Roche) under denaturing conditions ...
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bioRxiv - Cell Biology 2023Quote: ... blocked in 5% nonfat dry milk and proteins detected using monoclonal anti-GFP (Roche), monoclonal anti-PGK (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... His6-GFP and His6-mCherry proteins were purified with cOmplete His tag resin (Roche) according to the manufacturers protocols as previously described9 ...
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bioRxiv - Immunology 2023Quote: ... Pre-digested proteins and peptides were reduced with 10 mM dithiothreitol (DTT) (Roche, 10197777001) for 30 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... and protein was extracted using SDS lysis buffer93 with cOmplete protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5% (v/v) Triton X-100) containing 1X protein and phosSTOP inhibitor cocktails (Roche) for 5 min on ice and cleared by centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with 20 μL of resuspended volume of Protein G beads (Roche) for 2 h at 4 °C to pull down bound proteins ...
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bioRxiv - Immunology 2023Quote: ... Secreted IgGs were purified from cell supernatants with Protein A resin (Roche, Basel, Switzerland). IgGs were eluted at low pH using Protein A elution buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and organoids were resuspended in RIPA protein lysis buffer containing protease inhibitor cocktail (Roche) at day 7 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation of GFP/YFP-tagged proteins was performed with anti-GFP antibodies (11814460001, Roche) using a method we previously described (Ishii and Akiyoshi ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with rat α-HA (3F10, Roche), rabbit α-Myc (Cell Signaling) ...
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bioRxiv - Plant Biology 2022Quote: ... using an Ovation Ultralow V2 kit (NuGEN) or a Kapa DNA HyperPrep Kit (Roche) in combination with IDT UD barcodes (Illumina) ...
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bioRxiv - Developmental Biology 2023Quote: ... Riboprobes were synthesized by DIG RNA labeling kit and in vitro transcription kit (Roche Applied science ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were quantified using the KAPA Library Quanitification Kits - Complete kit (Universal) (Kapa Biosystems). Average size fragment was determined using a LabChip GXII (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... The normalization of DNA amount was performed by quantifying albumin gene copies with qPCR using human genomic DNA (20 ng/µl) standard (Roche, #11691112001) as we previously described16.
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bioRxiv - Microbiology 2019Quote: ... while all other specific primers were designed to be compatible with the Human Universal Probe Library set (90 probes, octamer, Roche Diagnostics) (Supplementary Table 9 ...
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bioRxiv - Immunology 2019Quote: ... the wells were washed five times and incubated with 100 µl/well goat anti-human IgG conjugated with alkaline phosphatase (Roche Diagnostics) diluted 1 in 5,000 for 30 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cultured with 2 μg/ml of plate-bound anti-CD3 and anti-CD28 monoclonal antibodies (αCD3αCD28 stimulation) (mAbs) (eBioscience) and 25 U/ml of recombinant human interleukin-2 (IL-2; Roche Applied Science) at a concentration of 1.5-2 × 106 cells/ml in RPMI supplemented with 10% heat-inactivated Human Serum (HS ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 30 min in Ringer solution before plating on fibronectin-coated glass coverslips (human plasma fibronectin at 10 µg/ml, Roche 10838039001).
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bioRxiv - Microbiology 2021Quote: ... and washed once in cell lysis buffer supplemented with 20U of human placental RNase inhibitor and cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) prior to processing lysates ...