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Citations for Roche :
6851 - 6900 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the cells were pelleted by centrifugation (750 x g, 2 min, RT) and washed in 4 ml vPBS containing EDTA-free protease inhibitor (Roche). The washed cells were again pelleted by centrifugation (750 x g ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation at 7000 xg for 15 minutes and were lysed in 10 ml PBS supplemented with protease inhibitor (cOmplete, Mini, EDTA-free, Roche). NatT was purified by immunoprecipitation using Anti-FLAG magnetic beads (Merk ...
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bioRxiv - Biophysics 2023Quote: Cell lysate powder from 12 L of cell culture was resuspended in 300 mL of room temperature eIF4G Lysis Buffer supplemented with 2 EDTA-free protease inhibitor tablets (Roche), 20mM imidazole ...
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bioRxiv - Cancer Biology 2023Quote: ... the skin from euthanized neonatal mice of indicated genotypes was incubated with 250 units/mL dispase (Roche, Catalog No. 04942078001) in KSFM medium (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... Tumor spheroids were digested into single cell suspensions using 0.028 Wünsch units/ml of Liberase TM Research Grade (Roche, Mannheim, Germany) and 20 μg/ml of DNase I in L-15 with 10 % CS for 1 hour at 37 °C and 250 RPM on an orbital shaker ...
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bioRxiv - Cell Biology 2024Quote: ... The minced hearts were transferred to digestion buffer (20mM BDM, 1.5mg/ml Roche Collagenase/Dispase enzyme mix in Leibovitz L15 medium)(Roche, 10269638001)(cellgro ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was dounce homogenized in a nuclear extraction buffer (10mM Tris, 146mM NaCl, 1mM CaCl2, 21mM MgCl2, 0.1% NP-40, 40u/mL Protector RNAse inhibitor (Roche 3335399001). Dounce homogenizer was washed with 4mL of a washing buffer (10mM Tris ...
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bioRxiv - Physiology 2024Quote: Dissected tips of digits and foot pads of mice were minced and incubated in 0.25mg/mL liberase TL (Roche, 5401119001) for 45 min at 37 °C with pipetting every 10min ...
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bioRxiv - Biochemistry 2024Quote: ... For lysis the pellets are thawed and resuspended in 2 ml of lysis buffer containing benzonase and cOmplete protease inhibitors (Roche), and processed as described for small scale expression in E ...
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bioRxiv - Cancer Biology 2024Quote: ... in buffer (10 mM Tris-Cl pH8.0, .25 mM EDTA, 100mM NaCl, 0.5% SDS 1mg/ml proteinase K) (Roche Cat #03508838103) at 55 °C for 4 hours to remove proteins ...
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bioRxiv - Cell Biology 2024Quote: ... The tissue was then washed twice in PBS with antibiotic and antimycotic and digested with 2mg/ml collagenase P (Roche) in DMEM (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... Stable cell lines were established by selection with Schneider’s Drosophila medium supplemented with 300µg/ml hygromycin (Roche, Palo Alto, CA).
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bioRxiv - Molecular Biology 2024Quote: ... samples were washed three times with 10 ml of ice-cold PBS supplemented with Complete Protease Inhibitor (Roche, Basel, Switzerland), and the cells were resuspended in 1 ml of the same buffer ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... cells were washed in PBS and lysed in 50 mL McIlvaine buffer containing EDTA-free cOmplete Mini Protease Inhibitors (Roche). Cells were shaken at 4°C and 450 rpm for 10 min followed by one hour of incubation at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was resuspended (20 mL/L culture volume) in lysis buffer (20 mM Tris pH 8, 150 mM NaCl, and protease inhibitor cocktail, Roche). These resuspended cells were disrupted using sonication (QSonica sonicator ...
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bioRxiv - Plant Biology 2024Quote: ... incubated three days at room temperature in the dark and then moved to selection media (CIM + Hygromycin (40 mg/ml final concentration, Roche) + Timentin (200 mg/ml final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... A 2ml Dounce homogenizer was used to individually homogenize each brain in a 1:1 solution of Lysis-R:2X-RIPA buffer solution with protease inhibitors (Roche cOmplete tablets #11836153001). Each sample was sonicated three times for 7 seconds and then centrifuged at 5000g for 5min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then incubated overnight at 4°C in primary antibody dilutions in freshly prepared BBT+ buffer (PBST + 1% BSA + 0.5 mM Spermidine + 2 mM EDTA + 1 large Roche complete EDTA-free tablets). Primary antibody was replaced with BBT+ buffer and quickly washed twice ...
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bioRxiv - Biochemistry 2021Quote: Bacteria were resuspended in 150 μL lysis buffer (1% Triton X100, 0.5% SDS, 1 tablet cOmplete EDTA-free protease inhibitor cocktail (Roche Diagnostics GmbH, Mannheim, Germany) in 10 mL PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... and sequentially incubated for 1 hour in a serum-based blocking solution and then for 1 hour in blocking solution containing the anti-digoxigenin (Roche, 11093274910; 1/2000) or anti-Fluorescein (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... The final pellet containing the chromatin fraction was resuspended in lysis buffer (10 mM HEPES, 500 mM NaCl, 1 mM EDTA, 1% NP-40, supplemented with protease and phosphatase inhibitor cocktails, Roche, and Benzonase, Millipore), sonicated 3 times at low amplitude ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membrane was blocked with 5% skim milk in TBS for 1 h and incubated with anti-HA-HRP (Roche, 3F10, 1:5000) in 5% milk/TBS ...
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bioRxiv - Biophysics 2021Quote: ... the cells were washed with cold 1X-PBS prior to collection with 800μL of chilled lysis buffer (50mM TRIS-HCl [pH=7.4]; 150mM NaCl; 1mM EDTA; 1mM CaCl2; 1mM MgCl2; 1% Triton, 0.5% sodium deoxycholate; protease inhibitor cocktail [Roche] 1 tablet/10mL lysis buffer). The cells were incubated on ice for 30 minutes and then centrifuged at 13,000 rpm/ 15minutes/ 4° C ...
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bioRxiv - Neuroscience 2020Quote: ... 40 mM KCl, 5 mM EGTA, 5 mM MgCl2, 5 mM DTT, 1 mM PMSF, 1% Triton X, protease inhibitor Roche complete, pH 7.2) and sonicated at low power for 5 s ...
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bioRxiv - Microbiology 2020Quote: NUP153/NSP1-expressing cell pellets were resuspended in lysis buffer (1× PBS, 0.5 mM TCEP, 0.1 mM PMSF, 1× Roche cOmplete protease inhibitors, pH 7.4), and lysed in a homogenizer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in 10 ml of extraction buffer (pH 6.7) (250 mM sucrose, 120 mM KCl, 10 mM MOPS, 5 mM MgCl2, 1 mM DTT, 1 Roche protease inhibitor cocktail tablet) (Vought et al ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were lysed in lysis buffer (50 mM Tris/HCl, 1 mM EDTA, 1% SDS, phosphatase inhibitor cocktail (Roche, Indianapolis, IN, USA), and protease inhibitor cocktail (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse IgG1 mAb α-GFP (1:500, Roche Diagnostics #11814460001), mouse IgG2 mAb α-Centrin clone 20H5 (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated overnight at 4°C with anti-Digoxigenin-AP-conjugated antibody (dilution range: 1:1000 to 1:5000, Roche, catalog no. 11093274910) diluted in the same buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... and estrous cycle phase were pooled in PBS and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The wells containing fixed cells were washed once with 1X PBS and stained with DAPI (50 μg/ml) for (Roche, 10236276001) 10 min at RT and protected from light ...
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bioRxiv - Immunology 2021Quote: ... was added to the PBMCs for 5 minutes before transferring drop-wise to a 10ml volume of warmed media (RPMI/20%FBS) containing 200U/ml of DNase I (Roche, Switzerland). After 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... For the neuraminidase treatment each well was supplied with 500 μL of a solution containing 50 mU/mL neuraminidase (Roche Diagnostics) in cold PBS+++ pH adjusted to 5.5 ...
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bioRxiv - Zoology 2019Quote: ... TBST washes of 15 minute each at room temperature were performed thrice and subsequently the tissue was stained using NBT (working concentration 500ug/ml; Roche #11383213001) and BCIP (working concentration 562.5ug/ml ...
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bioRxiv - Genomics 2020Quote: ... tissue was cut into 1mm x 1mm cubes and placed in 0.25mg/ml liberase TH dissociation medium (Roche Diagnostics, Indianapolis, USA). Following further dissection ...
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bioRxiv - Immunology 2021Quote: ... the lamina propria was dissected into small pieces with scissors and incubated in digestion buffer (RPMI 1640 containing 0.17 mg/mL Liberase TL (Roche, Basel, Switzerland) and 30 µg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cultured with 2 μg/ml of plate-bound anti-CD3 and anti-CD28 monoclonal antibodies (αCD3αCD28 stimulation) (mAbs) (eBioscience) and 25 U/ml of recombinant human interleukin-2 (IL-2; Roche Applied Science) at a concentration of 1.5-2 × 106 cells/ml in RPMI supplemented with 10% heat-inactivated Human Serum (HS ...
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bioRxiv - Cancer Biology 2020Quote: Neurosphere cell cultures and PC3 were synchronized at metaphase by treating with 80 ng/ml Colcemid (Roche, 10-295-892-001) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 30 min in Ringer solution before plating on fibronectin-coated glass coverslips (human plasma fibronectin at 10 µg/ml, Roche 10838039001).
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bioRxiv - Biochemistry 2022Quote: ... The plasmid was transfected into Neuro2a cells and clonal lines selected by incubation with Hygromycin (300 mg/ml, Roche (Nutley, NJ)) ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM Tris-HCl (pH 8.0) and protease inhibitor (one tablet of Roche cOmplete™ mini per 10 ml lysis buffer) [22] ...
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bioRxiv - Microbiology 2021Quote: The following agents have been used in the study: cotrimoxazole (Bactrim, sulfamethoxazole and trimethoprim 150/ 15 mg/ mL; Roche, Basel, Switzerland), artesunate (10 mg/ mL ...
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bioRxiv - Neuroscience 2021Quote: ... Briefly, ganglia from adult Aplysia were treated with Dispase® II (10 units/ml) (neutral protease, grade II) (Roche, Indianapolis, IL) at 35°C for ∼3 h and then desheathed ...
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bioRxiv - Cancer Biology 2021Quote: ... The material was collected in a 50mL centrifuge tube and supplemented with 0.01 mg/mL Liberase TL (Roche, Woerden, the Netherlands); the suspended tumor tissue was incubated at 37°C for 3-5 hours while shaking at 250 rpm ...
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bioRxiv - Cancer Biology 2021Quote: HS and healthy skin tissues were cut into small pieces and were digested with 2.5U/ml Dispase-II (Roche, Cat#04942078001) in Hanks’ Balanced Salt Solution (HBSS ...
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bioRxiv - Immunology 2021Quote: ... We resuspended the glomerular fraction in 1mL HBSS buffer with Ca2+ and Mg2+ (Hyclone, Logan, UT) containing 0.1-0.2 mg/mL Liberase™Research Grade (Roche, Mannheim, Germany), and incubated glomeruli in 24-well tissue culture plates at 37°C for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by perfusion with Hanks’ balanced salt solution with Mg2+ and Ca2+ containing 0.1 mg/mL of Liberase (Roche Applied Science). Following the removal of the liver ...