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Citations for Roche :
6701 - 6750 of 7579 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Proteases were inactivated by the addition of 1 mM PMSF and 10 ul 1x complete protease inhibitor cocktail (Roche).
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bioRxiv - Microbiology 2024Quote: ... resuspended in 4 ml of a buffer (HEPES 10 mM pH 7.4, 1 mM EDTA, EDTA-free protease inhibitor cocktail from a tablet (Roche)) and lysed by 3 passages through a cell disrupter at 2.4 kbar ...
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bioRxiv - Immunology 2024Quote: ... Tissues were then incubated for 1.5 hours at 37°C in an orbital shaker (200 rpm) with 1 ml of digestion media containing 0.25 mg/ml Liberase TL (Roche) and 0.25 mg/ml DNase I (Sigma ...
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bioRxiv - Immunology 2024Quote: ... brachial and mesenteric lymph nodes from 3 mice/genotype were pooled and cut into pieces and digested with the 1 ml of enzyme mix containing 0.8 mg/ml Dispase (Roche), 0.2 mg/ml Collagenase P (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... Plant tissues were ground in liquid nitrogen and the powder was suspended in lysis buffer (50 mM Tris-HCl at pH 8.0, 150 mM NaCl, 1 mM phenylmethanesulfonyl fluoride, 10 mM iodoacetamide, and 1X Roche cOmplete mini protease inhibitor cocktail) ...
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bioRxiv - Immunology 2024Quote: ... Brain stems were cut into pieces and digested for 40 min at 37 °C with 5% CO2 in PBS supplemented with 1.6 IU ml-1 Collagenase/Dispase (Roche) and 0.5 mg ml-1 DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% NP-40 (vol/vol) supplemented with protease inhibitor cocktail (cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, Cat# 05892791001) for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... cells were lysed in IPH buffer (20mM Tris-HCl pH7.5, 150mM NaCl, 2mM EDTA, 1% NP-40) supplemented with protease inhibitor cocktail (Roche, cOmpleteTM EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended with 1 ml of single nuclei wash buffer containing 0.2 U/µl RNase inhibitor (3335402001, Roche) and transferred to a microcentrifuge tube ...
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bioRxiv - Biochemistry 2024Quote: His-tagged 53BP1 TTD was purified by resuspending thawed cell pellets in 30ml of lysis buffer (50mM sodium phosphate pH 7.2, 50mM NaCl, 30mM imidazole, 1 x EDTA free protease inhibitor cocktail (Roche) per litre of culture) ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 1µg of DNAse from bovine pancreas and 1 dissolved tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Lysates were incubated with Ni-NTA agarose (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 1µg of DNase from bovine pancreas and 1 dissolved tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Pellets were then homogenized with a Biospec Tissue Tearor (Dremel) ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using NP-40 lysis buffer (100 mM Tris pH 8.0, 1% NP-40, 150mM NaCl) containing protease inhibitor cocktail (Roche) for 20 min with shaking on ice ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then harvested by centrifugation and resuspended in buffer A (20 mM Tris-HCl, 1 mM EDTA, pH 8.0) including a protease inhibitor tablet (EDTA-free, Roche). Cells were lysed by sonication on ice before cell debris was removed by centrifuged (18,000 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellets were resuspended in 150 μl chilled lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.1, 1x protease inhibitor cocktail, 1x PhosSTOP phosphatase inhibitors (Roche), 5 mM sodium butyrate (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 1/10 volume of lysis buffer (0.2 M Tris-HCl, pH 8, 10 mg/mL lysozyme, and 1× EDTA-free protease inhibitors; [Roche]), incubated for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and supplemented with 850 μl 1% Triton X IP dilution buffer (1% Triton X, 20mM Tris-HCl pH8.1, 150mM NaCl, 2mM EDTA, 1x protease inhibitor cocktail (Roche), 1x PhosSTOP phosphatase inhibitors (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... 12 mM MgCl2 and 150 mM KCl in RNAse free water; 0.5 mM DTT, 1 µL/mL Protease Inhibitors Cocktail [Roche] ...
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bioRxiv - Microbiology 2024Quote: ... and 10% glycerol) supplemented with the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche), frozen in liquid nitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Cells were lysed in DDM lysis buffer (50 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM EDTA; 50 mM NaF; 1mM sodium vanadate; 1x Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2024Quote: ... each sample was normalized to 3.5 ng in 5 µL water before generating libraries using a 1:10 Kapa HyperPlus (Roche) miniaturized protocol with barcoded indices as previously described82 ...
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bioRxiv - Molecular Biology 2024Quote: ... Dicer was detected using anti-HA 3F10 monoclonal primary antibody (High Affinity rat IgG1, Roche #11867431001; dilution 1:500), anti-HA rabbit primary antibody (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... 500 μg cell lysate was diluted to 500 μL in lysis buffer supplemented with 1 mM DTT and 25 μg poly(dI:dC) (Roche) and precleared by incubating with 50 μL streptavidin beads for 1 hour at 4°C on rotating wheel ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extraction buffer (150 mM NaCl, 1% Triton x-100, 50 mM TrisHCl pH 7.4 with protease inhibitor (11836170001 Roche)) ...
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bioRxiv - Physiology 2024Quote: ... cells were lysed in IP buffer (10 mM Tris pH 8, 0.4% NP40, 300 mM NaCl, 10% glycerol, 1 mM DTT, antiprotease (Roche), anti-phosphatase (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... Aggregate fractionation buffer (100 mM HEPES pH 7.4, 1% Triton X-100, 300 mM NaCl) supplemented with Protease Inhibitor Cocktail (Roche) and 25 mM N-ethylmaleimide was added at 3 volumes per unit mass of dried seedling weight ...
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bioRxiv - Neuroscience 2024Quote: Each brain hemisphere was added to a 7 mL Precellys tube with pre-loaded zirconium oxide beads (Precellys, Bertin, USA) and homogenized in ice cold 0.02% CHAPS in 1x PBS with protease inhibitors (1 Roche cOmplete tablet 4693159001 ...
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bioRxiv - Neuroscience 2024Quote: ... protease inhibitor (1 tablet per 10 ml solution; cOmplete ULTRA Tablets-Mini, F. Hoffmann-La Roche AG, Basel, Switzerland) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells are then washed twice in cold 1% BSA solution containing 0.5X EDTA-free Protease inhibitor cocktail (PIC, Roche), and once in cold PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were washed twice with cold PBS before resuspending in ChIP shearing buffer (50 mM Tris HCl pH 8.0, 1% SDS, 10 mM EDTA with protease and phosphatase inhibitors, Roche). Samples were sonicated using a Covaris ME220 for 7.5 minutes using the following sonication conditions ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were co-lysed together in lysis buffer (40mM Tris-HCl pH 7.5, 500mM NaCl and 1 mM DTT) with 100 mM Pefabloc (Roche) and protease inhibitor cocktail (Roche cOmplete EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was resuspended in lysis buffer (80 mL per L of initial culture: 20 mM HEPES pH 8, 500 mM NaCl, 5% v/v glycerol, 0.5 mM TCEP + 1 Roche cOmplete EDTA-free protease inhibitor tablet ...
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bioRxiv - Cancer Biology 2024Quote: ... and MAZ (1:25, Immunoway Cat# YT2669) was performed using the Ventana BenchMark ULTRA instrument (Ventana/Roche, AZ, USA) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... and the remaining cell pellet was lysed at a final concentration of 1.25×106 cells/mL in lysis buffer (1% NP40-PBS containing protease and phosphatase inhibitors (Roche)) and stored at -20°C until further analysis.
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 20 mM Imidazole, 1 mM TCEP, and protease inhibitors from Roche), and lysed by several passes through a microfluidizer (LM20 Microfluidizer ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody-bound beads were washed in BSA-PBS (0.5%) before the addition of pre-cleared lysate in 1% Triton with protease inhibitor (Roche). Following rotation overnight at 4 °C the beads were washed in RIPA (50 mM HEPES pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 3× protease inhibitor cocktail [Roche cOmplete]), and lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Cell Biology 2024Quote: ... islets were preincubated for 1 hour in KRBH buffer containing 0.1% (w/v) bovine serum albumin (BSA) (10775835001, Roche) and 3 mM glucose before incubation with 11 mM glucose ± 100 nM GLP-1RAs in KRBH buffer in a shaking 37°C water bath (80 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and resuspended in lysis buffer (50 mM HEPES pH 8.0, 300 mM NaCl, 1 mM TCEP, supplemented with Roche EDTA-free cOmplete protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2024Quote: Equal amounts of total RNA (1 µg) were reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with random hexamer primers for one hour at 50°C ...
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bioRxiv - Cell Biology 2024Quote: ... The nuclear pellet was washed once with 3mL ST buffer and then twice with 3mL FANS buffer (1% BSA, 1mM DTT, and 0.2U/μL RNAse inhibitor (03 335 402 001, Protector RNAse inhibitor, Roche) in RNAse free DPBS (AM9624 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacteria pellets were resuspended in Lysis Buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM DTT, 0.1% Triton-X, and protease inhibitors, cOmplete Mini, EDTA-free, Roche, #4693159001). Next ...
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bioRxiv - Cell Biology 2024Quote: ... and thawing and by resuspension in 5 mL of ice-cold Lysis/Wash buffer (25 mM HEPES pH 7.5, 1 M NaCl, 5% glycerol) supplemented with cOmplete protease inhibitor cocktail (Roche), followed by vigorous sonication on ice delivered by a Jencons Ultrasonic Processor (15s bursts at 70% amplitude) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM Tris/HCl pH8) to which protease inhibitors were freshly added (1 tablet of Complete protease inhibitor per 25 ml, Roche, Mannheim ...
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bioRxiv - Cancer Biology 2021Quote: ... 150 mM NaCl, 1 mM PMSF, 1x protease inhibitor cocktail without EDTA from WAKO, 1x phosphatase inhibitor cocktail from Roche) containing 20 mM N-Ethylmaleimide to prevent further cysteine oxidation during protein extraction ...
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bioRxiv - Cell Biology 2020Quote: Cells were harvested using a cell scraper and were lysed with 200 μl/well of 0.1% CHAPS lysis buffer (50 mM Tris-HCl, 150 mM NaCl and 1 mM EDTA) supplemented with a Mini Protease inhibitor cocktail (Roche). The cell lysate was incubated on ice for 15 mins with occasional mixing before centrifugation at 20,000 g at 4°C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... The pellets were washed once with Buffer A and then further lysed with 250 μl modified RIPA buffer (1mM EDTA, 150mM NaCl, 0.1% Na-DOC, 1% NP-40, 50Mm Tris pH7.5 and Complete Roche protein inhibitors). 50U of benzonase (nuclease ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were allowed to air dry fully before applying Hybridizing Solution (70% formamide, 1 mg/ml blocking reagent (Roche), 10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Developmental Biology 2021Quote: Wildtype stain and drl:mCherry-positive zebrafish embryos were grown until tailbud stage and chorions were removed by incubating in 1 mg/mL Pronase (Roche), followed by washing in E3 medium ...