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Citations for Roche :
6601 - 6650 of 7579 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Cell lysates were extracted using Igepal lysis buffer (1% Igepal, 150mM NaCl, 50mM Tris pH 7.4) enriched with protease inhibitor cocktail (Roche). Proteins were analyzed by SDS-PAGE and immunoblotting.
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bioRxiv - Molecular Biology 2023Quote: 3-5 day old males of the indicated genotypes and experimental groups were homogenised in 150µl of homogenisation buffer (0.2%Triton X-100 supplemented with 1 x complete mini EDTA-free protease inhibitor (Roche) in PBS ...
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bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
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bioRxiv - Neuroscience 2023Quote: ... NaCl 150 mM supplemented with 1% Triton X-100 in the presence of protease inhibitors (Complete, Roche Diagnostics GmbH). Fusion proteins were purified on glutathione-Sepharose (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were centrifuged at 1,000 x g for 1 min at RT prior to loading on a LightCycler 480 (Roche) using 3-step cycling ...
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bioRxiv - Cancer Biology 2023Quote: ... The total RT-qPCR reaction volume was 1.5⍰μL and consisted of 1⍰μL of LightCycler 480 SYBR Green I Master Mix (Roche) containing 0.5⍰μM of PCR primer (Eurogenetec ...
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bioRxiv - Cell Biology 2023Quote: ... Pellet was resuspended in high-salt buffer (750 mM NaCl, 10 mM Tris, pH 7.6, 1 mM EDTA, protease inhibitor tablet (Roche)) and lysed by sonication at 60% power using a probe sonicator (Branson sonifier 450 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM DTT, 0.4% Igepal CA-630, 1 mM MgCl2, 200 µg/ml lysozyme, 10 µg/ml pepstatin A, Roche cOmplete protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) or Lysis buffer (1 % SDS, 50 mM Tris pH6.8 and 10 % Glycerol) supplemented with protease inhibitor cocktail (Roche) and subsequently quantified using Pierce BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Aortas were manually homogenized and digested for 20 minutes at 37°C in RPMI with 1 mg/mL of collagenase A and collagenase B and 0.1 mg/mL DNAse I (Roche). Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche ...
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bioRxiv - Microbiology 2023Quote: ... and lysed using NP-40 lysis buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 30 minutes on ice ...
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bioRxiv - Physiology 2023Quote: ... or 50 Tris-HCl pH 7.5, 1 phenylmethylsulfonyl fluoride, 3% SDS (RyR, SERCA, CaMKIIδ-C273S) and supplemented with cOmplete protease inhibitor (Roche). Lysates were then incubated on ice for 15 min and centrifuged for 15 min at 15000 rcf at 4°C ...
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bioRxiv - Genomics 2023Quote: ... and the pellet resuspended in 100 µl of ice-cold RIPA lysis buffer containing 1× protease inhibitor cocktail (Roche), incubated for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed by sonication in 700 mM NaCl;20 mM HEPES 7.5 containing 1 protease inhibitor tablet (Roche) + 5ul Turbonuclease ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of OsHV-1 and total bacteria were performed using quantitative PCR (qPCR, Roche LightCycler 480 Real-Time thermocycler) with the following program ...
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bioRxiv - Cell Biology 2023Quote: ... cardiomyocytes were dissociated using DPBS for 20 min at 37 °C followed by 1:200 Liberase TH (Roche, 5401151001) diluted in DPBS for 20 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibodies were incubated for 1 h at 37°C while nuclei were counterstained with DAPI (Roche) at RT for 5 min before mounting the glass coverslips using DAKO mounting medium (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... washed and resuspended in lysis buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM MgCl2; 10% Glycerol; Complete protease inhibitor (Roche); 1 mM PMSF or 2 mM AEBSF-hydrochloride (BioChemica APA1421.0001) ...
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bioRxiv - Biophysics 2022Quote: ... 5 EDTA and 1% Triton X-100) supplemented with cOmplete™ Mini-EDTA free protease inhibitor cocktail (Roche, Germany). Lysates were incubated 5 min on ice and centrifuged 10 min - 14000 x g at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analysed by qRT-PCR on a LightCycler 480 using SYBR Green 1 Master Mix (Roche). Relative fold change in expression was determined by the ddCt method ...
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bioRxiv - Cancer Biology 2023Quote: ... Each suspension was then supplemented with 0.1 mL of RPMI containing 1.25 mg mL−1 Liberase TM (Roche, 5401119001) and 20 mg mL−1 DNase I (Roche ...
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bioRxiv - Genetics 2023Quote: Mouse tissue was homogenized in 400 μl of RIPA homogenizing buffer (50-mM Tris HCl pH 7.4, 150-nM NaCl, 1-mM EDTA, supplemented with protease-inhibitor cocktails (Roche)) and lysed with a MagNA Lyser ...
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bioRxiv - Genomics 2023Quote: Fresh pinch biopsies were washed twice in DPBS before being coarsely chopped with a scalpel and transferred into 0.5 mL DPBS solution containing 0.1 WU ml−1 Liberase TH (Roche, 5401151001) and 0.5 U ml−1 RQ1 DNase I (Promega ...
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bioRxiv - Immunology 2023Quote: 50,000 sorted cells were washed with PBS and resuspended in Buffer 1 (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... released by trypsin and incubated in ice-cold lysis buffer (Tris-HCl pH 8.0, 50mM, NaCl 150mM, EDTA 1mM, NP40 1%, cOmplete Mini Protease Inhibitor Cocktail (Roche) and phosphatase inhibitor PhosSTOP (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then released by trypsin and lysed in ice-cold lysis buffer (Tris-HCl pH 8.0, 50mM, NaCl 150mM, EDTA 1mM, NP40 1%, cOmplete Mini Protease Inhibitor Cocktail (Roche) and phosphatase inhibitor PhosSTOP (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... Western blotting was conducted as described above and visualized with anti HA-HRP antibody (Roche 39 #12013819001; 1:2,000).
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bioRxiv - Developmental Biology 2023Quote: ... Minced lung was enzymatically dissociated to a single cell suspension using 1 mg/mL collagenase A (Roche, Cat# 10103578001), 2-4U/mL elastase (Worthington ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001], PhosSTOP [Roche, PHOSS-RO] ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
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bioRxiv - Plant Biology 2023Quote: ... 5% glycerol, 50mM KCl, 5 mM EDTA, 0.1% Triton X-100, 5 mM DTT and 1 × protease inhibitor cocktail [Roche]). The homogenized lysates were incubated at 4 °C for 1 hour for western blot analysis of eIFiso4G1 or overnight for mass spectrometry analyses with 50 µl of prewashed immobilized γ-aminophenyl-m7GTP (C10-spacer)-agarose beads (Jena Bioscience) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Immunology 2023Quote: ... enzymatic digestion of tissue pieces was done with collagenase D (1 mg/ml) and DNAseI (0.1 mg/ml) (both from Roche) in HBSS/10%FBS for 45 min (37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in lysis buffer (300 mM KCl, 1 mM MgCl2, 0.1 mM ATP, 1X Roche Protease/PPase inhibitor ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in immunoprecipitation (IP) buffer (1× PBS, 0.1% Triton X-100, and cOmplete EDTA-free protease inhibitor cocktail [Roche]) and then disrupted by three pulses of sonication for 5 s ...
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bioRxiv - Molecular Biology 2023Quote: ... pellets were resuspended in 100 μl of lysis buffer (50 mM Tris, pH 7.5, 1 mM EDTA, 3 mM DTT, and 1X cOmplete protease inhibitor cocktail [11836145001, Roche]) and lysed on a beadbeater for 5 min at room temperature with 100 μl of acid-washed glass beads ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were taken up in sonication buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS, cOmplete protease inhibitor by Roche) at a ratio of 10 million cells in 150 µL (120 uL for ChIP-seq) ...
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bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
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bioRxiv - Plant Biology 2023Quote: ... pH 7.0, 1 mM EDTA, 10 mM DTT, 0.05% [v/v] Triton X-100 and 1x Protease Inhibitor Cocktail [Roche]). Crude leaf extracts were incubated for 2 h at 4 °C on a turning wheel and centrifuged at 16.900 g for 5 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were first mechanically dissociated with a scalpel into approximately 1-3 mm pieces and enzymatically digested in RPMI-1640 medium (Cytiva) containing 0.25 mg/ml DNase I (Roche) and 0.25 mg/ml Collagenase (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM NaCl, 1% SDS, 0.1 mM dithiothreitol, 20 μM PMSF, 0.5% β-mercaptoethanol, 1X proteinase inhibitor from Roche) and centrifuged for 10 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Nonidet P-40) supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail tablets (1 tablet/10ml) (11836170001, Roche) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Bacterial pellets were harvested by centrifugation and resuspended in 50 mL of lysis buffer (20 mM Tris pH 7.5, 500 mM NaCl) to which 1 protease inhibitor tablet was added (Roche). Bacterial cells were lysed by sonication with 10 s pulses for 10 min and cell debris was removed by centrifugation at 25,000 x g for 40 min and cleared cell lysate was filtered through 0.45 μm filters ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... coli were harvested and disrupted by sonication in the lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP40) supplemented with protease inhibitor cocktail (cOmplete, Roche). After centrifugation at 40000 g for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: Lungs extracted from E14.5 or E18.5 embryos were minced and digested using collagenase I and dispase I (both 1 mg/mL, Roche) and incubated at 37°C for 5 min ...