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Citations for Roche :
601 - 650 of 3928 citations for 8 4 Chlorophenylthio 2' O methyladenosine 3' 5' cyclic monophosphate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 5 mM EGTA, 1 mM DTT, 2 mM MgCl, and one EDTA-free protease inhibitor cocktail tablet; Roche) and sonicated in an ice bath for 6 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... glands were minced into paste and incubated in 5 mL DME/F-12 medium (HyClone, #SH30023.1) with 2 mg/mL collagenase A (Roche #10103578001), 100 units/mL hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in HR buffer (50 mM Tris-HCl, 5 mM MgCl2, 250 mM sucrose, 2 mM TCEP and protease inhibitor t (Roche), pH 7.4) ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Developmental Biology 2023Quote: ... with probe prepared by nick translation of a double-stranded PCR product bearing the bcd ORF using alkali-stable digoxigenin-11-2’-deoxyuridine-5’-triphosphate (Roche), visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Immunology 2023Quote: ... 3 mM ATP (Roche), 25 μg/ml MSU (InvivoGen) ...
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bioRxiv - Plant Biology 2021Quote: ... was isolated from the cDNA of juvenile cluster root tissues from P-deficient plants using the 5’/3’ RACE Kit (2nd generation, Roche Diagnostics S.p.a., Monza, Italy) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and phoC-A-R1 (5’-CAA CAT CGC TTT GCC AGT G-3’) (Fraser et al., 2017) with a Roche LightCycler® 96 (Roche Diagnostics, Mannheim, Germany) using 5 µL KAPA SYBR FAST 2x qPCR Master Mix (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... For isolation of nuclei the cell pellet was resuspended in Buffer 1 (10 mM Tris-HCl pH=7.5, 2 mM MgCl2, 3 mM CaCl2, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 20 min followed by a centrifugation step ...
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bioRxiv - Neuroscience 2022Quote: ... 20 mg protein was loaded onto 8-10% SDS-PAGE gels for electrophoresis and subsequently transferred (90 V, 2-3 h) onto PVDF western blotting membranes (Roche, Cat. No. 3010040001) using the Criterion™ System BioRad ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 10 mL of L2 buffer (200mM NaCl 1mM EDTA pH 8 0.5mM EGTA 10mM Tris, pH 8, dH2O, 1 protease inhibitor tablet (Roche Complete cat #1 697 498) per 50ml buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... beads were resuspended in low-salt rinse buffer (20 mM HEPES, pH7.5, 0.5 mM spermidine, a Roche mini-complete tablet per 10 ml and 0.05% Digitonin) ...
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bioRxiv - Cell Biology 2021Quote: ... and digested in Han s’ balanced salt solution containing 0.18 units/mL collagenase (10 mg/mL; Roche) for 45min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The cross-linking reaction was quenched by incubating the cells with 0.125 M glycine for 5 min with mild agitation at room temperature followed by centrifugation at 3,000 rpm for 5 min at 4°C with a subsequent PBS wash (containing 0.01X protease inhibitor cocktail or PIC; #11836170001, Roche Applied Science, Indianapolis, USA). Following the complete removal of PBS ...
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bioRxiv - Microbiology 2019Quote: ... 8 mM EDTA) with Complete™ protease inhibitor cocktail (Roche). The Triton X-100-insoluble material was pelleted twice by centrifugation (10 min at 16,000 g) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and phenol (phenol-Tris saturated, pH 8; Roche, Indianapolis, IN) and chloroform:isoamyl alcohol (American Bioanalytical ...
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bioRxiv - Biochemistry 2022Quote: ... pH=8) supplemented with EDTA-free protease inhibitor mixture (Roche) and 100 mM PMSF ...
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bioRxiv - Microbiology 2023Quote: ... and suspended in 8 M urea containing PhosSTOP (Roche Diagnostics) and Benzonase (Novagen) ...
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bioRxiv - Biophysics 2023Quote: Microchambers were incubated with 8 mg/µl anti-digoxigenin (Roche) at 25°C for 90 min and then the blocking buffer (PBS with 1% α-casein ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% sodium deoxycholate) with cOmplete™ Mini Protease Inhibitor Cocktail (Roche, 11836153001) on ice for 30min ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% sodium deoxycholate supplemented with protease inhibitors (Hoffmann-La Roche, Basel, Switzerland) for 10 min at 4° C ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5 mM sodium deoxycholate) supplemented with protease- and phosphatase inhibitors (Roche, Switzerland) by mechanical homogenization on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5% sodium dodecyl sulfate supplemented with EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5% sodium deoxycholate) supplemented with protease inhibitor mixture (Roche Diagnostics, COEDTAF-RO). Lysates were incubated for 30 minutes at 4 °C on rotator and cleared at 12000xg for 20 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... 16mM sodium fluoride and one tablet of EDTA-free protease inhibitors (Roche) per 50 ml) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% sodium dodecyl sulfate) supplemented with cOmplete™ protease inhibitor cocktail (Roche) and incubated for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% sodium dodecyl sulfate supplemented with EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% (w/v) sodium deoxycholate) with Complete Mini protease inhibitor (Roche Diagnostics) and Histone deacetylase inhibitors (iHDAC ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... 0.5 % sodium deoxycholate) supplemented with complete protease inhibitor (Roche Diagnostics, Basel, Switzerland) and phosphatase inhibitor (PhosStop ...
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bioRxiv - Systems Biology 2023Quote: ... 0.1% sodium dodecyl sulphate) supplemented with 1x proteinase inhibitor cocktail (Roche, #11836153001). Proteins were separated on 4-15% gradient gels (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.5% Sodium Deoxycholate) with Protease Inhibitor cocktail (cat no. 046693124001, Roche) and 1μM PMSF (cat no ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS and 0.1% Sodium deoxycholate) supplemented with protease inhibitor cocktail (Roche) and quantified by BCA protein assay (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecylsulfate (w/v)) supplemented with 1x protease inhibitor cocktail (Roche). Lysates were clarified by centrifugation at 20,000 x G for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 1mM sodium orthovanadate) supplemented with protease inhibitor tablets (Roche, cat. no. 04693159001). Protein concentration was measured using a Pierce BCA Protein Assay (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5% sodium deoxycholate) supplemented with protease inhibitor complex (PIC) (Roche, Grenzach, Germany), 0.1% SDS ...
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bioRxiv - Immunology 2023Quote: ... 0.5% sodium deoxycholate) supplemented with cOmplete™ and PhosSTOP inhibitors™ (Roche) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.1% sodium dodecyl sulphate) supplemented with protease inhibitor cocktail tablets (Roche). 4x Laemmli reducing buffer was added to samples before heating at 95°C for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5% sodium deoxycholate 0.5% Triton X-100) with protease inhibitor (Roche, 11836170001). Protein quantification was done by Pierce BCA protein assay following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
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bioRxiv - Physiology 2021Quote: ... 1:500 goat anti-rabbit (AlexaFlour 488; A-11008) and 1:1000 4′,6-diamidino-2-phenylindole (DAPI; Roche 10236276001; Basel, Switzerland). After secondary incubations ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was transferred to a 50 mL conical tube and resuspended in DPBS/BSA containing collagenase B (2-4 mg/mL) (Roche, Mannheim, Germany) and DNase I (2,000 U/mL ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
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bioRxiv - Plant Biology 2019Quote: ... Total proteins from 120 seedlings were extracted with 150 µL of extraction buffer (50 mM Tris-HCl pH 7.4, 80 mM NaCl, 0.1 % Tween 20, 10 % glycerol, 10 mM dithiothreitol, 2× Protease inhibitor cocktail [11873580001, Roche], 5 mM PMSF). Prior to protein quantification ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 cycles of primary PCR to amplify the region of interest was performed using 2 μL of DNeasy eluate (∼100–300 ng template) in a 5 μL Kapa HiFi HotStart polymerase reaction (Kapa Biosystems; for primers see Supplementary Data Set 1) ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification involved cDNA (2 μl, 5 ng/μl) in optical 384-well plates (Roche Light Cycler 480; Roche Diagnostics, Mannheim, Germany) with SYBR Green I dye and gene-specific primers (Supplemental Table S8) ...
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bioRxiv - Cancer Biology 2020Quote: Cell (1 × 104) proliferation was measured using the 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit III (Roche, Mannheim, Germany) (Jin ...
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bioRxiv - Neuroscience 2019Quote: ... 0.5% Triton X-100, 100 mM NaF, 1 mM ortho-vanadate, 2 mM EDTA, and a protease inhibitor cocktail [Roche 11836153001]). Lysates were vortexed and cleared by centrifugation (15,000 x g for 15 min at 4°C) ...
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bioRxiv - Cell Biology 2022Quote: ... of siCon or siRictor NIH3T3 cells co-expressing Flag-NDRG1 WT were homogenized in 2% SDS + 5 mM DTT to retrieve proteins in solution supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...