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Citations for Roche :
6251 - 6300 of 6311 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Developmental Biology 2020Quote: ... DIGprobes were detected with antibodies in MABT containing 5% horse serum appropriate for NBT/BCIP in situ hybridization (Roche anti-DIG-AP 1:1000) or for FISH (Roche anti-DIG-POD 1:1000) ...
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bioRxiv - Neuroscience 2021Quote: ... whereas mouse primary cortical cultures and activated T lymphocytes were lysed in lysis buffer (50 mM Tris, 150 mM NaCl, 1% Triton X-100) containing protease and phosphatase inhibitors (Roche, New York, NY, USA). Total protein contents were quantified using the BCA assay (Pierce) ...
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bioRxiv - Genetics 2022Quote: ... Embryos were then washed 3x 5 minutes in TBS-T (1% Tween-20 in Tris-buffered saline) and blocked for 1 hour in block solution (10% heat-inactivated sheep serum and 0.1% Roche blocking reagent in TBS-T). Roche blocking reagent was dissolved in maleic acid buffer according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse transcribed using Transcriptor High Fidelity cDNA Synthesis Kit and a specific 3’-UTR DENV-1 primer (Roche Applied Science, Mannheim, Germany), d1a5B 5’-AGAACCTGTTGATTCAACRGC-3’ (62) ...
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bioRxiv - Biochemistry 2020Quote: ... Cell pellets were resuspended in lysis buffer (50 mM HEPES-NaOH, pH 7.5, 500 mM NaCl, 5% v/v glycerol, 1 mM TCEP-NaOH, Roche protease inhibitor cocktail EDTA-free) at the ratio of 50 ml / L equiv ...
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bioRxiv - Developmental Biology 2020Quote: ... hybridized with sense or antisense probes overnight at 65 °C and incubated with the alkaline phosphatase-coupled sheep anti-digoxigenin antibody (1:2000, Roche Applied Science, Manheim, Germany) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: Ovaries and testis were fixed and stained according to standard procedures with the following primary antibodies: rat anti-HA (1:500, Roche cat# 11867423001, RRID: AB_390919), rat anti-Vasa (1:100 ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed in Tris-Buffered Saline (TBS) + 1% Triton X-100 with EDTA and protease and phosphatase inhibitor cocktails (Roche Diagnostics, Florham Park, NJ). To remove insoluble materials ...
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bioRxiv - Genomics 2020Quote: ... the tissues were cut into 1-2 mm2 pieces and put in 1mg/ml collagenase and dispase (C/D) solution (Roche Diagnostics GmbH Roche Applied Science, Mannheim, Germany) at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... and homogenized in 5 volumes of ice-cold PBS containing 1% NP-40 and Complete® protease inhibitor cocktail tablets (Roche Diagnostics, Basel, Switzerland) using 2×10 clockwise strokes with 5 s rest time ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 μL of each primer at 10 μM and 0.5 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc, A Roche Company) for a total volume of 25 μL ...
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... against foxg1 was carried out as previously described by Thisse (Thisse and Thisse, 2008) The antibody used to detect the riboprobes was AP-anti-DIG (Roche Cat.N: 11093274910, 1:500). For Fluorescent WM ISH (WM FISH) ...
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bioRxiv - Biochemistry 2023Quote: ... in presence of MBP (10 μg) in the kinase assay buffer (50 mM Tris, 50 mM MgCl2, 1× phosphatase inhibitor cocktail (Roche Life Science, Indianapolis, IN), 1 mM DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... Frozen bone marrow cells from MPN patients or frozen leukopaks from healthy donors were thawed and incubated overnight in RPMI media with DNase 1 (Roche 10104159001, 0.1 mg/mL). Freshly collected bone marrow samples from some MPN patients were used directly for cell isolation ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions were supplemented with SDS (to a final concentration of 1%) and then digested with proteinase K (Roche, final concentration of 2 µg/ml) for 45 min at 42 C ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ng of recombinant PfCDPK1 protein was taken in a kinase assay buffer (50 mM Tris, 50 mM MgCl2, 1× phosphatase inhibitor cocktail (Roche Life Science, Indianapolis, IN), 1 mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in non-denaturing buffer at 4°C (10 mM Tris, 500 mM NaCl, MgCl2 1mM, 3U of DNase I, and protease inhibitor cocktail 1 X from Roche, ref 4693116001, pH 7.9) followed by sonication and ultracentrifugation at 13000 rpm for 45 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and were resuspended in 30 mL lysis buffer 1 (50 mM sodium phosphate, 300 mM NaCl, pH 8) containing 2 mL of EDTA-free protease inhibitor mixture (Roche Applied Science, Penzberg, Germany). Cells were disrupted by passing the cell suspension three times through a Constant Cell disruption system (TS benchtop ...
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bioRxiv - Biophysics 2024Quote: ... to a final molal concentration of 25 ng/μL and amplified using primers specific to each oligonucleotide subpool with KAPA SYBR FAST qPCR (KAPA Biosystems; 1-ng template). A second PCR amplification was performed with KAPA HiFi (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellet was rinsed with 500 µL of resuspension buffer (20 mM Tris pH 8, 75 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.125 mM PMSF, 50 % glycerol, 1x Roche complete, 20 U/mL RNase Out) and centrifuged at 4000 xg at 4°C for 3 min ...
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bioRxiv - Cell Biology 2024Quote: ... a final concentration of 1 ng/μl cDNA was mixed with 2 μl FastStart DNA Master SYBR Green I (Roche #03 003 230 001), 0.5 μM forward and reverse primer ...
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bioRxiv - Neuroscience 2023Quote: DRGs were collected from both WT and KO mice (8 weeks male) and were incubated in 1 mg/ml Collagenase/Dispase (Roche Diagnostics, Madison, WI, USA) at 37℃ for 60 min ...
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bioRxiv - Cell Biology 2023Quote: ... Sectioned zebrafish muscle tissue was rinsed in phosphate buffered saline (PBS) for 15 min before the addition of blocking solution (1% blocking reagent (Roche Diagnostics GmBH, Manheim, Germany) with 0.4% Triton X ...
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bioRxiv - Neuroscience 2024Quote: ... resuspended in 150 μL buffer C (50 mM Tris pH 8, 5 mM EDTA, 1% SDS, 100 mM NaCl, 1x Roche complete mini protease inhibitors) and incubated on ice for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... mCIT tagged proteins were revealed by using respectively GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche; at 1/1000 in 5 % milk over-night) as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG ...
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bioRxiv - Plant Biology 2022Quote: ... 1/100 cDNA dilutions were analysed using a LightCycler®480 and LightCycler®480 SYBR Green I Master (Roche, Welwyn Garden City, UK) following manufacturer’s instructions and cycling conditions of pre-incubation 95°C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Genetics 2024Quote: ... 30 mM Tris-HCl, 20 mM KCl, 2 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 150 mM NaCl, cOmplete proteinase inhibitor [Roche Diagnostics, Indianapolis, IN, USA]), lysed by ultrasonic treatment and incubated with EZview anti-HA agarose beads (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and digested for 45min in digestion solution composed of Hanks’ balanced salt solution supplemented with collagenase D (1 mg/ml, Roche Diagnostics cat # 54 11088882001) and deoxyribonuclease (DNase ...
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bioRxiv - Plant Biology 2024Quote: ... The powder was dissolved and thawed in a lysis buffer (100 mM Tris/HCl pH 8, 150 mM NaCl, 0.5 mM TCEP, 1 tablet Roche Protease inhibitor/50 mL solution) using 1 ml of buffer per g of powder ...
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bioRxiv - Developmental Biology 2024Quote: ... L2 and L3 larvae were collected in a Tris-lysis buffer (10 mM Tris, 150 mM NaCl, 1% Triton X-100, 25 mM EDTA added of EDTA-free Roche complete protease inhibitor cocktail) and homogenized to extract soluble proteins ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with load dilution buffer (solubilization buffer supplemented with 1% (w/v) sodium cholate) and loaded on a Ni-NTA affinity column (Roche cOmplete Ni-NTA resin) equilibrated with 10 CVs load dilution buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in ice-cold lysis buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10% Glycerol, 1% Triton X-100, cOmpleteTM protease inhibitor cocktail tablet from Roche and a PhosSTOP tablet from Roche). Lysates containing 500-1000 μg protein (made up to 500 μl by lysis buffer ...
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bioRxiv - Immunology 2021Quote: Single cells were sorted directly into PCR tubes containing 25 μl PCR lysis buffer (1x Buffer 1 from the Expand Long Template PCR System [Roche], 0.5 mg/ml Proteinase K) and lysed for 1h at 55°C ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1 mM CaCl2, 5 mM EGTA, 50 mM sucrose, 0.1% Triton X-100, 1/2 tablet of cOmplete inhibitor [Roche], and 2.5 U/mL Benzonase [Novagen]), lysed by sonication ...
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bioRxiv - Neuroscience 2020Quote: Sections were treated with 2% H2O2 in PBS to inactivate endogenous peroxidase followed by incubation with anti-DIG antibodies POD fragments in combination with anti-DNP POD (Roche, Basel Switzerland; dilution 1:1000). Incubations with these antibodies were done separately at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... except that all digests contained 200 µg/mL fibrils and 40 µg/mL pronase E and that the digest was stopped in each 20 µL aliquot with 2 µL protease inhibitor cocktail solution (1 tablet cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, in 2 ml pure water) instead of PMSF solution ...
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bioRxiv - Neuroscience 2023Quote: ... 500 mM NaCl, 1 mM MgCl2, 10 mM Imidazole, 0.5% IGEPAL® CA-630, 2 mM β-Mercapto-ethanol supplemented with Roche cOmplete inhibitor cocktail tablets) at a ratio of 15 ml of buffer/g of biomass ...
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bioRxiv - Neuroscience 2023Quote: ... Cultured cells were harvested at different time points as indicated in the figures and lysed in 1% Triton X-100 buffer containing protease inhibitor cocktail (Roche diagnostics, # 11-836-170-001), 1 mM PMSF ...
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bioRxiv - Plant Biology 2023Quote: First-strand cDNA was synthesized by reverse transcribing 1 μg of total RNA in a final reaction volume of 20 μl using MMLV reverse transcriptase (Roche High Fidelity cDNA synthesis kit) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: A teratoma was cut into smallest possible pieces and dissociated for 1 hour at +37C in a collagenase A solution (Roche, 0.1 mg/ml in DMEM). The resulting cell suspension was passed through mesh strainers with pore diameter from 100 μm to 40 μm ...
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bioRxiv - Molecular Biology 2024Quote: Cell pellets were lysed in a lysis buffer (50 mM HEPES, 250 mM NaCl, 0.5 mM EDTA, 1% NP-40, 1X Roche Protease Inhibitor Cocktail, 10 mM DTT) for 5 min with gentle rocking ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 µl of 1x Lysis Buffer were added (20 mM Tris-HCl pH8, 150 mM NaCl, 2 mM EDTA, 1x Protease Inhibitor Roche, #04693116001, 1% NP40 Sigma, #I8896), the embryos were smashed by pipetting up and down several times and the samples were centrifuged at 4 °C at maximum speed for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... At the end of the cell disruption dry homogenised powder of cells were resuspended in 1 ml urea buffer (8 M urea, 50 mM Tris/HCl pH 8.0 containing one tablet of ‘cOmplete’ protease inhibitor per 25 ml, Roche, Germany; 1 mM DTT; 0.1 M PMSF) and centrifuged at 16,000 × g for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... using the primers indicated in Table 1 after cDNA synthesis using random hexamer primers and Roche AMV reverse transcriptase (10109118001 Roche, Sigma-Aldrich, St. Louis, MO, USA). Samples were multiplexed using Takara long amplicon Taq polymerase with GC buffers following the manufacturer’s instructions for PCR barcoding for nanopore sequencing (EXP-PBC096 Oxford Nanopore Technologies ...
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bioRxiv - Pathology 2021Quote: ... Tissue sections were then blocked with blocking solution buffer II (containing 0.1% Triton X-100 and 2% normal sheep serum) at room temperature for 30 min before covering with anti-DIG alkaline phosphatase conjugate anti-digoxigenin antibody (Roche, diluted 1:500 in buffer II) for 1 h at 45°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Each cell pellet was resuspended in 35 mL of lysis buffer (50 mM Na-phosphate buffer pH 8.0, 500 mM NaCl, 14 mM β-mercaptoethanol, protease inhibitors [1 Roche Mini EDTA-free tablet/10 mL]). Cells were disrupted by sonication (4 times for 1 min with intermittent cooling on ice) ...
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bioRxiv - Neuroscience 2023Quote: ... and resuspended in lysis buffer (20 mM Tris-HCl, 1% Triton detergent, 10% glycerol, 2 mM EDTA, 137 mM NaCl, and Roche EDTA-free protease inhibitor, pH 7.4). Cells were homogenized and protein content assessed by Bradford assay ...