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Citations for Roche :
6151 - 6200 of 6311 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and 1 tablet of protease and phosphatase inhibitors per 50 mL (cOmplete™ ULTRA Tablets Mini EDTA-free and PhosSTOP, Roche Diagnostics) and sonicated with Vibracell (10 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed in ice-cold 1X PBS before being scraped into 200 µL of ice-cold 1X PBS containing 0.1% NP-40 (1 tablet of the cOmplete Protease Inhibitor Cocktail (Roche, Cat No. 11697498001) and 1 tablet of the PhosSTOP Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... 25 µg/ml digitonin and 1 M hexylene glycol) supplemented with protease inhibitor cocktail (cOmplete Mini EDTA-free tablets, Roche, Basel, Switzerland) for 10 min at 7°C rotating ...
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bioRxiv - Bioengineering 2024Quote: ... Specific cell types within these sections were identified using a series of primary antibodies: 1:500 rabbit anti-ERG (Roche, Basel, Switzerland) for endothelial cells ...
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bioRxiv - Cell Biology 2024Quote: ... and 1% Triton X-100) with either 1mM CaCl2 or 1mM EGTA supplemented with complete protease and phosphatase inhibitors (Roche, Mannheim, Germany). Lysates were subjected to two rounds of sonication for 10 s each ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were plated one day before transfection and were then incubated with a 2:1 ratio (μL:μg) of X-tremeGENE™ HP DNA Transfection Reagent (Roche, Ref: 6366236001) and plasmids for 20 min in Opti-MEM (GibcoTM ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked in 5% milk in PBS with 0.001% Tween-20 (PBST) before incubation with the specified antibodies prepared in 5% milk (GFP (Roche, 11814460001, 1:1,000), FLAG-HRP (Merck ...
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bioRxiv - Evolutionary Biology 2024Quote: ... subsequently resuspended in 200µl of Lysis Buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche #04693132001)) and frozen with liquid nitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... pH 8.0 50 mL / 5 M NaCl 30 mL / 5 g Sodium Deoxycholate / 1 g SDS / 1 L SDS g / NP-40 10 mL / MQ up to 1 L) containing 1x protease inhibitor (cOmplete™, EDTA-free Protease Inhibitor Cocktail [Roche, 16829900]). After centrifugation at 10,000 G ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were harvested by centrifugation at 6,000 g for 10 min at 4°C and resuspended in L Buffer (25 mM Tris-HCl, 500 mM NaCl, 10 mM Imidazole, 1 mM PMSF, 5 % Glycerol, pH 8, containing Roche protease inhibitor cocktail). Bacteria were then lysed by sonication ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cell lysates were prepared in radioimmuno-precipitation assay (RIPA) buffer supplemented with 1 tablet of EDTA-free protease inhibitor (Roche, Cat No. C762Q77) per 25ml buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then quickly washed twice in wash+ buffer (20 mM HEPES pH7.5 + 150 mM NaCl + 0.1% BSA + 0.5 mM Spermidine + 1 large Roche complete EDTA-free tablets in water). Samples were resuspended in 150 μl Wash+C (wash+ + 100 mM CaCl2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Pelleted nuclei were resuspended in 333 μl of nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS, 1x Roche Complete protease inhibitors cocktail), kept 5 min on ice and diluted with 667 μl of ChIP dilution buffer (16.7 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... expressing polymerase module and SII-tagged Mpe1 were lysed in Buffer A (50 mM HEPES pH 8, 75 mM NaCl, 1 mM TCEP) supplemented with 3x protease inhibitor tablets (Roche, cat. No. 11836153001) and 1 ml BioLock ...
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bioRxiv - Genetics 2020Quote: ... After 24 hours cells were then fixed with a 1% (v/v) formaldehyde solution supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche®, UK) for 15 minutes with gentle agitation at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 72°C for 1 min) including the TruSeq primer mix and the KAPA Hifi Uracil+ Poly-merase Master Mix (Kapa Biosystems, Cat. #KK2801). The PCR product was purified and size controlled by Agilent Technologies 2100 Bioanalyzer (High Sensitivity DNA Chip) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen) and SOX9 (1:10000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were pelleted in a centrifuge and lysed with lysis buffer (20mM Tris pH 7.5, 1% Triton, 1mg/mL Lysozyme, 5mM DTT and protease inhibitor cocktail, Roche #11-836-170 001) for 1 hour at 4 °C ...
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bioRxiv - Physiology 2021Quote: 50 islets per sample were washed in PBS twice and then lysed and sonicated in RIPA buffer (10mM HEPES, 50mM β-glycerol phosphate, 1% Triton X-100) supplemented with complete mini protease inhibitor cocktail (Roche, Laval, QC) and phosphatase inhibitors (2mM EGTA ...
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bioRxiv - Physiology 2020Quote: ... Inhibitors were added fresh on the day of use and included 1 cOmplete™ protease inhibitor cocktail EDTA free tablet (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail 3 both purchased from Sigma-Aldrich (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgOAc, 5 %w/v glycerol, 0.25 %w/v NP-40, 3 mM DTT, 1 mM PMSF, Roche protease inhibitor cocktail (PIC), pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... along with the appropriate antibody (mouse HA 12CA5, 7.5 μl 0.4 mg/ml, Roche; mouse M2 FLAG, 5 μl 1 mg/ml, Sigma), before incubating at 4 °C on a rotating wheel at 14 rpm for 15-18 h ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested and resuspended in lysis buffer A (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM PMSF, and Roche EDTA free protease inhibitor). Following sonication and centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... and the cDNA template was digested for 30 min at 37 °C after the addition of 1 µl DNase I (10 U/µl; Roche Diagnostics, Rotkreuz, Switzerland), 5 µl 1M Tris/HCl buffer (pH 8.0) ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with high salt HKT buffer (25mM HEPES pH 7.2, 1M NaCl, 1% Triton X-100, 2mM DTT, 1x Protease Inhibitor (Roche, cOmplete™ EDTA free)) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were collected and incubated in HKT lysis buffer (25mM HEPES pH 7.2, 150mM KCl, 1% Triton X-100, 2mM DTT, 1x Protease Inhibitor (Roche, cOmplete™ EDTA free)) for 30 minutes on ice prior to homogenization with a 25-gauge syringe needle ...
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bioRxiv - Cell Biology 2022Quote: Cells were washed once with ice-cold PBS and stored at -80 °C or immediately lysed using lysis buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% NP-40, 2x Roche EDTA-free protease inhibitors). Lysates were collected by scraping and were cleared by centrifugation at 16,100 g for 10 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Plant Biology 2021Quote: ... total protein was isolated from Agrobacterium infiltrated leaves using extraction buffer (50mM Tris-Cl, 1% β-Mercaptoethanol and protease inhibitor cocktail (Roche, Basel, Switzerland)) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by mechanical and enzymatic digestion with 150 μL collagenase/dispase (1 mg/mL) and 300 μL DNAse (10 mg/mL; both Roche diagnostics, IN, USA) for 45 min at 37 °C with mild agitation ...
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bioRxiv - Biochemistry 2020Quote: ... The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol, 10 mM Tris-HCl pH 7.4) containing protease inhibitors (complete protease inhibitor cocktail, Roche; 1 tablet per 50 mL), and lysed by 10 strokes in a Dounce homogenizer ...
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bioRxiv - Genetics 2022Quote: ... Samples were suspended in Laemmli sample buffer supplemented with a protease and phosphatase inhibitor cocktail (PIC; 1 mM PMSF, cOmplete™ Protease Inhibitor Cocktail [Roche, Base, Switzerland], PhosSTOP [Roche, Base, Switzerland]), and 10 mM N-ethyl maleimide (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with cold PBS and resuspended in NE1 buffer (20 mM HEPES-KOH pH7.9, 10 mM KCl, 1mM MgCl2, 1 mM DTT, 0.1% triton X-100 supplemented with Roche complete protease inhibitor EDTA-free) and rotated for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was then incubated in antibody detection solution consisting of 1:20000 anti-HA rat mAb conjugated to horseradish peroxidase (HRP) (Roche [3F10], Cat. # 1201381900) diluted in MTBST 0.05% for 1 hour at room temperature on a tube rotator ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in IP buffer (150 mM NaCl, 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice using ice-cold D-PBS before scraping in ice-cold RIPA lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40 alternative, complete protease inhibitor and phosphatase inhibitor [Roche Applied Science, Mannheim, Germany]). The lysate was incubated on ice for 30 minutes with gradual agitation before clarification of samples by centrifugation at 10,000 ×g for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Pellets from exponentially grown cultures of Pea2-9myc expressing yeast cells were resuspended in yeast extraction buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA) with 1x protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany), and vortexed together with glass beads (3-fold amount of glass beads and extraction buffer to pellet weight ...
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bioRxiv - Cancer Biology 2021Quote: ... one 10 cm plate of Hepa1c1c7 cells at 80% confluence was lysed in 1 mL Pierce IP Lysis Buffer (supplemented with 100 U/mL SUPERase-IN and 1X Roche protease inhibitor cocktail) and incubated on ice for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... For isolation of nuclei the cell pellet was resuspended in Buffer 1 (10 mM Tris-HCl pH=7.5, 2 mM MgCl2, 3 mM CaCl2, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 20 min followed by a centrifugation step ...
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bioRxiv - Physiology 2020Quote: ... and immunodetection of the DIG-labeled hybrids was performed with a peroxidase-conjugated anti-DIG antibody (1:2000; raised in sheep; Roche Diagnostics, Mannheim, GER). The fluorescence signal was developed with tyramide signal amplification (TSA ...
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bioRxiv - Systems Biology 2020Quote: ... The final pellet was resuspended in 150 μl of NTE buffer (137 mM NaCl, 1 mM EDTA, 10 mM Tris, pH 7.4) with protease inhibitor (Roche, cOmplete Mini tablets, 4693159001). In ultracentrifugation library experiments ...
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bioRxiv - Cell Biology 2020Quote: ... and then digested for 1 h at 37°C in a vigorously shaking solution consisting of 16 mg of Collagenase D (Roche, Pleasanton, CA, USA), 3.0 U of Dispase II (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 5 mM EDTA, 50 mM NaF, 1 mM PMSF supplemented with Roche 1X Halt Protease inhibitor cocktail), incubated for 1 hour at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: Cardiac myofibrils were prepared from left ventricular trabecular strips pre-stretched overnight to a sarcomere length of about 2 µm in rigor buffer (20 mM HEPES pH 7, 140 mM KCl, 2 mM MgCl2, 2 mM EGTA, 1 mM DTT, Roche complete protease inhibitor) at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and lysed using immunoprecipitation buffer (25 mM Tris pH 7.4, 150 mM NaCl, 0.4% NP40, 5% glycerol, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF). Approximately 1.5 mg of lysate from each sample was incubated with brachyury antibody at 4°C for 4 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pooled together and resuspended in 1.5 mL immunoprecipitation buffer (25 mM Tris pH 7.4, 150 mM NaCl, 0.4% NP40, 5% glycerol, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF). Cells were then incubated for 30 minutes on ice prior to centrifugation at high speed (15,000 rpm ...
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bioRxiv - Neuroscience 2021Quote: Cells were washed in cold D-PBS prior to lysis on ice using lysis buffer (1% NP40, 100mM Tris pH8, 100mM NaCl, 10% glycerol, 5mM EDTA, cOmplete™ EDTA-free Protease Inhibitor Mixture, Roche Applied Science) supplemented with 20 mM N-ethylmaleimide ...
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bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s instruction and 1 microG of total RNA was reverse transcribed into cDNAs using the first-strand synthesis kit (Roche, Tucson, AZ, USA). Real-time quantitative PCR analyses were performed according to a previously published procedure 39 ...
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bioRxiv - Immunology 2020Quote: ... at 37° C 5% CO2 in 96 well plates in the presence of IL2 (GIBCO 100 IU/mL or Roche 1 ng/mL). Additional conditions included 1 ng/mL TGFβ (GIBCO) ...