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Citations for Roche :
6201 - 6250 of 6963 citations for Rat Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The equally mixed pool of VH and Vκ/Vλ gene fragments was subjected to overlap extension PCR to generate scFv genes using a KAPA Biosystems kit (Roche) with overlap extension primers (No ...
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bioRxiv - Plant Biology 2023Quote: ... Paromomycin probe was labeled with dUTP-DIG during PCR reaction (PCR DIG probe synthesis kit, Roche; see table primers list). Membrane was pre-hybridized 1h at 50°C in a DIG eazy Hyb (Roche ...
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bioRxiv - Genomics 2023Quote: ... Multiplexed library pool was based on HS D1000 and Kapa Library Quantification Kit for Illumina platforms (Kapa BioSystems, Boston, MA) and sequenced at shallow depths on Illumina’s iSeq 100 v2 flow cell for 28×10×10×90 cycles for estimated reads per cell ...
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bioRxiv - Plant Biology 2024Quote: ... Post-hybridization washes and immuno-chemiluminescent detection of the bound probe were conducted following the instructions provided in the DIG Northern Starter Kit manual (Roche). To assess siRNA size via northern blot analysis ...
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bioRxiv - Biochemistry 2023Quote: ... was used for cDNA synthesis and RT-qPCR was performed using KAPA SYBR FAST qRT PCR kit (KK4602, KAPA Biosystems). Taf10 was used as a control for normalisation and the fold change in mRNA levels were calculated by 2^-ddct method.
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of the reverse transcrip-tion mix from the Titan One Tube RT-PCR System kit (Roche, Switzerland) was added ...
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bioRxiv - Microbiology 2023Quote: ... five microliter of elution was loaded into a 96-well plate with the Light Cycler RNA master hybridization Probe kit (Roche) and amplification was performed in a Lightcycler 480 machine (Roche ...
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bioRxiv - Immunology 2024Quote: ... ATP concentration in cell lysates was measured in a FB 12 Luminometer (Berthold detection systems) using the ATP Bioluminiscence Assay Kit CLS II (Roche).
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bioRxiv - Immunology 2024Quote: ... 1 μg of total RNA was reverse transcribed using the Roche Transcriptor First Strand cDNA synthesis kit (Roche Life Sciences) according to manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... Blots were then exposed to 2.5 pmol of 40-nt HPLC purified DNA oligonucleotides conjugated to digoxigenin (DIG) using the DIG Oligonucleotide Tailing Kit (Roche) hybridized to the nitrocellulose membrane at 60°C overnight (42°C for 2 h for 5S rRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries for RNA-Seq of PARCB with PGC-1α overexpression and control samples were prepared with KAPA Stranded mRNA-Seq Kit (Cat# KK8420, Roche). The workflow consists of mRNA enrichment and fragmentation ...
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bioRxiv - Neuroscience 2024Quote: ... Digoxigenin (DIG)-labeled sense and antisense probes were generated with T7 and Sp6 RNA polymerase using a DIG RNA labeling kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed using the first-strand cDNAs diluted 5-fold in water and KAPA SYBR FAST qPCR Master Mix (2x) kit (Roche) and gene-specific primers in a LightCycler 96 (Roche).
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bioRxiv - Cell Biology 2024Quote: ... The reported concentration of 500 ng was used as input for the KAPA Hyper Library Preparation Kit (Kapa Biosystems KK8504) according to the manufacturer’s instructions with 8 cycles of PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were serially applied using the U DISCOVERY 5-Plex IF procedure and the following reagents and kits (all from Ventana Medical Systems, Roche): DISCOVERY inhibitor (760-4840) ...
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bioRxiv - Developmental Biology 2024Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (11684795910, Roche), commonly ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from dCasRx+gRNA transfected HEK293T cells was reverse transcribed using oligo dT and the Transcriptor First Strand cDNA Synthesis Kit (#04897030001, Roche) as manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Sheared DNA was subjected to the modified genomic DNA library protocol using the Kapa Hyper Prep Library Kit (Kapa Biosystems) described by Faircloth et al ...
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bioRxiv - Molecular Biology 2024Quote: ... Indexed libraries that met appropriate cutoffs for both were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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Development and Initial Characterization of Pigs with DNAI1 Mutations and Primary Ciliary DyskinesiabioRxiv - Physiology 2024Quote: ... The tissue was lysed using the KAPA Express Extract Kit Plus Amplification Module and KAPA2G polymerase (KAPA Biosystems, Basel Switzerland). The forward primer (5’-GAT GTT TAT CAC TGT GGC AAG GAT TCA GT-3’ ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were made using Roche KAPA Biosystems protocol (KAPA RNA Hyper Prep Kit Technical Data Sheet, KR1352 – v4.17, Roche Corporate) and the quality was evaluated ...
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bioRxiv - Cell Biology 2024Quote: Apoptosis was measured by Terminal Deoxynucleotidyl Transferase-Mediated dUTP-Biotin Nick End Labeling (TUNEL) assay using In Situ Cell Death Detection kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The quality of finished libraries were checked on Agilent 2100 Bioanalyzer using High sensitivity DNA chip and the quantity of libraries were measured by qPCR using KAPA Library Quantification kit (Roche). Libraries were pooled with equal amount and sequenced on an Illumina Novaseq sequencer by single-end 100 bp sequencing.
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bioRxiv - Immunology 2024Quote: ... Quantitative RT-PCR analysis was performed with selective primers (Supplementary Table 1) using the Light Cycler 480 SYBR Green I Master X2 Kit (Roche), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... For Bulk RNASeq 500 ng of lung RNA/sample were used to prepare libraries for sequencing by using KAPA RNA HyperPrep Kits (Roche). Libraries generated with IAV-infected samples were sequenced by Macrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... mostly from an input of at least 100 ng of DNA using the KAPA HyperPrep Kit (Roche Diagnostics, Rotkreuz, Switzerland) and the SureSelect XT (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... the extracted DNA was ligated to specific adaptors for Illumina’s HiSeq system using the KAPA Hyper Prep Kit (Kapa Biosystems).
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bioRxiv - Immunology 2024Quote: ... and then i5/i7 barcoded primers were added to tagmented cDNA using Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (Roche) to barcode amplicons ...
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bioRxiv - Immunology 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Immunology 2024Quote: ... Extracted RNA samples underwent library preparation for polyA tailed mRNA with a RNA concentration of >1ng/1ul using with Kapa RNA hyperPrep kit (Roche), followed by 75 cycles of Next-generation sequencing with NextSeq® (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Illumina-compatible sequencing libraries were prepared by using a modified version of KAPA HyperPrep library kit (KAPA BioSystems, Willmington, MA). Quality check steps were performed to assess the fraction of proximally ligated DNA labeled with biotin ...
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bioRxiv - Molecular Biology 2024Quote: ... were subjected to library preparation after rRNA depletion and poly(A)+ RNA enrichment using the Kapa Hyper Stranded mRNA library kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Digoxigenin-labeled RNA probes based on the sequence of VRN1 were synthesized using a DIG northern Starter Kit (Roche, 11277073910), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Extracted RNA samples underwent library preparation for total RNA sequencing in samples where the pre-ribodepletion RNA was >1ng/1ul using with Kapa RNA hyperPrep kit (Roche), followed by 75 cycles of Next-generation sequencing with NextSeq® (Illumina ...
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bioRxiv - Microbiology 2024Quote: Extracted RNA samples underwent library preparation for total RNA sequencing in samples where the pre-ribodepletion RNA was >1ng/1ul using with Kapa RNA hyperPrep kit (Roche), followed by 75 cycles of Next-generation sequencing with NextSeq® (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... the DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems) and purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Plant Biology 2019Quote: ... Adapter-ligated cDNA fragments were enriched and amplified with 15 cycles of PCR using the HiFi NGS Library Amplification kit from KAPA Biosystems. Libraries with were multiplexed at equimolar concentration and sequenced on the Illumina HiSeq 2500 for 1×40 bases ...
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bioRxiv - Cancer Biology 2021Quote: ... Library qualities and sizes were checked with the TapeStation 4200 and then quantified using the KAPA Library Quantification Kit for Illumina platforms (Kapa Biosystems). Libraries were sequenced on the Illumina NextSeq500 sequencer to a depth of about 50 millions of 75-bp pair reads per library.
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bioRxiv - Cancer Biology 2021Quote: ... RT-PCR was performed using first strand synthesis with specific primers (Reagents) and SYBR FAST qPCR Kit (Kapa Biosystems, Wilmington, MA) in a StepOne cycler (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Microbiology 2020Quote: ... The fragments were treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA Illumina Library prep kit (KAPA biosystems). Libraries for the rest of the samples were prepared in 96-well plates ...
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bioRxiv - Developmental Biology 2021Quote: ... Illumina-compatible sequencing libraries were prepared by using a modified version of the KAPA HyperPrep library kit (KAPA BioSystems, Willmington, MA). Quality check steps were performed to assess the fraction of proximally ligated DNA labeled with biotin ...
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bioRxiv - Cell Biology 2020Quote: ... Each RNA-seq library was analyzed by the fragment analyzer (Advanced Analytical Technologies) and quantified by KAPA Library Quantification Kit (KAPA Biosystems) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR reactions were performed with fast Start Universal Probe Master kit and UPL-probe system in a LightCycler 480 Instrument (Roche Diagnostics) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... with on-column DNase digestion followed by cDNA synthesis using the Transcriptor First Strand cDNA synthesis kit (Roche Molecular Diagnostics, 04379012001) using random primers ...
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bioRxiv - Immunology 2021Quote: Approximately 200-500 ng of FFPE RNA extracted from FFPE slides with a DV200 range between 3-99 or 65-100 ng of fresh frozen RNA (DV200 98-99) per sample were used for RNA library construction using the KAPA RNA Hyper library prep kit (Roche, Switzerland). The number of pre-capture PCR cycles was adjusted based on the quality and quantity of RNA extracted from the samples ...
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bioRxiv - Neuroscience 2020Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche-KK4824). Individual libraries were normalized to 1.30 nM in preparation for Illumina sequence analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... The library size distribution was checked on an Agilent high-sensitivity DNA chip and the library quantified using the KAPA library quantification kit (KAPA Biosystems). For each reaction ...
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bioRxiv - Genetics 2021Quote: ... PCR products were cleaned using Ampure XP Beads (Agencourt) and quantified using the KAPA Library Quantification Kit for Illumina® Platforms (Roche). Libraries were sequenced on a NextSeq sequencer (Illumina ...