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Citations for Roche :
6101 - 6150 of 6383 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Cells were washed with ice-cold PBS and lysed in RIPA buffer (50 mM pH 8.0 Tris-HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM NaVO4, 1mM NaF, Roche complete protease inhibitor tablet). Lysates were cleared by centrifuging at 16,000 Xg at 4°C for 10 m ...
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bioRxiv - Microbiology 2020Quote: ... endogenous peroxidase activity was blocked by adding H2O2 and incubating for 10 minutes followed by 2 more washes with PBS-tween and blocking by PBSK [PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk (Easis ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse hind limb muscles (tibialis anterior, quadriceps and gastrocnemius) were minced and incubated in a 1 mg/ml collagenase/dispase solution (Roche, Basel, Switzerland) with agitation at 37°C for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... The PBMCs were fused with cells from the mouse–human fusion partner cell line SPYMEG (Medical & Biological Laboratories, Nagoya, Japan) at a ratio of 10:1 with polyethylene glycol (Roche, Basel, Switzerland). Fused cells were cultured in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Developmental Biology 2021Quote: ... Revelation of the dig-labeled ISH probe was then performed using an anti-Digoxigenin antibody coupled to POD (11207733910; Roche, 1/400) and revealed using a FITC-tyramide (1/400) ...
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bioRxiv - Microbiology 2020Quote: ... G355-5 cells were transfected with 1 to 6 µg DNA in 6 cm dishes using X-treameGENE 9 (Roche, Basel, Switzerland). The 293T/17 cell line was obtained from ATCC (CRL-11268 ...
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bioRxiv - Cancer Biology 2021Quote: ... tumor tissues up to 1 g were mechanically minced and digested in 2 ml RPMI1640 with 200 µg/ml Liberase TL (Roche by MilliporeSigma), 200 µg/ml Liberase DL (Roche) ...
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bioRxiv - Biophysics 2022Quote: ... and mixed with 1 mL Ni Sepharose 6Fast Flow (Cytiva, Tokyo Japan) (MinD) or cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland) (MinE and msfGFP-MinC) ...
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bioRxiv - Developmental Biology 2022Quote: Single color staining of embryos hybridized with DIG-labelled riboprobes was performed using anti-DIG antibody conjugated to alkaline phosphatase (Roche, 1:6000) and BM purple (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight with 1:1,000 diluted alkaline phosphatase-conjugated anti-DIG sheep antibody (11-093-274-910; Roche Diagnostics, Basel, Switzerland) and then reacted with 0.375 mg/mL nitroblue tetrazolium and 0.188 mg/mL 5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP ...
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bioRxiv - Microbiology 2022Quote: ... by the capillary method and hybridised with digoxigenin-labelled blaOXA- 58 and blaNDM-1-specific probes with an NBT/BCIP colour detection kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) in the presence of protease inhibitors (Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Suspensions were flash frozen in droplets and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Microbiology 2022Quote: ... and 1 mM EDTA) supplemented with 150 mM NaCl and the Complete™ Mini EDTA-free protease inhibitor mixture (Roche Applied Bioscience) and 1mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were lysed with an IP lysis buffer (150 mM NaCl, 25 mM Tris-HCl, 10% glycerol, and 1 mM EDTA) containing a protease inhibitor cocktail (Roche, Basel, Switzerland). IP was performed with an anti-Prdx5 antibody (Ab Frontier) ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfected cells were lysed for 20 minutes in pre-chilled Co-Immunoprecipitation buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 0.53% NP-40) supplemented with 1×protease inhibitor (Roche-Sigma, Cat no. # 11873580001) and 1×phosphatase inhibitor (Thermo Scientific™ ...
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bioRxiv - Microbiology 2022Quote: ... 0.85 mg/ml; Collagenase D, Roche, 11088882001, 1.25 mg/ml; dispase, Gibco, 17105-041, 1 mg/ml; DNase, Roche, 101104159001, 30µg/ml). Tissues were then incubated at 37 °C with shaking for at least 15 min and manually shaken every 5 minutes until complete digestion ...
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bioRxiv - Cancer Biology 2022Quote: Primary mouse MEC organoids were prepared by manual chopping of mouse mammary gland tissues harvested from 10–12-week-old mice followed by digestion with shaking for 1 hr at 37 °C with Collagenase A from Clostridium histolyticum (10 mg/mL; Roche, Cat. #: 13560925) and Hyaluronidase from bovine testes (1 mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked with 10% lamb serum in TBST for 1 hour at RT and incubated with alkaline phosphatase conjugated to DIG antibody (Roche, Basel, Switzerland) in TBST ...
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bioRxiv - Microbiology 2020Quote: ... washed once with PBS and suspended in Homogenization Buffer (HB: 250 mM sucrose, Sigma-Aldrich; 10 mM HEPES pH 7.4, Sigma-Aldrich; 1 mM EDTA, Sigma-Aldrich, 2 mM PMSF, Roche). Homogenization was performed by a single freeze/thaw cycle ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of TruSeq PCR primer cocktail (50 μM) and 20 μl of KAPA HiFi HotStart ReadyMix (KAPA Biosystems, cat. no. KK2601). USER digestion was performed at 37 °C for 15 min ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... containing Roche mini-complete protease inhibitor EDTA-free and Roche PhosSTOP (1 tablet of each per 10 mL of buffer) (Roche, Basel, Switzerland). Samples (on ice ...
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bioRxiv - Biochemistry 2021Quote: ... for 10 min and then harvested using 1 ml of ice-cold 1X PBS supplemented with 0.01% Phosphatase inhibitor (PhosSTOP, Roche, Cat. no. 04906845001). Pellets corresponding to 10 plates of a 10 cm plate were pooled together and centrifuged at 5,000 rpm for 5 min at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (Anti-Digoxigenin-AP, Fab Fragments, 1:5000, Roche, Cat# 11093274910). Finally ...
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bioRxiv - Microbiology 2021Quote: Bacteria-captured beads were incubated at 37°C for 1 hour with 10 mg/mL of lysozyme (Cat # 10837059001, Roche, Basel, Switzerland) diluted in 10 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Lung lobes were then placed in 1 milliliter Triazol and homogenized with zirconia beads with a MagNA Lyser instrument (Roche Life Science).
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bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
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bioRxiv - Bioengineering 2019Quote: ... Fluorescence scanning was performed from 25°C- 95°C at a rate of 1°C/min using a Lightcycler 480 Instrument (Roche Life Scientific). Melting temperatures were calculated from the inflection point in the first-derivative curve.
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed 5 μl of crude lysate with 45 μl of TNG buffer (100 mM Tris, 100 mM NaCl, 10% glycerol, 10 mM DTT, 1×cOmplete™ (EDTA-free Protease Inhibitor Cocktail, Roche 11873580001), pH 7.2-7.5) ...
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bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were lysed in Tris-lysis buffer (10 mM Tris-Cl pH 7.5, 100 mM NaCl, 1% Triton X-100, 10% glycerol, and cOmplete™ protease inhibitor cocktail [Roche, Basel, Switzerland]) on ice ...
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bioRxiv - Epidemiology 2020Quote: ... plasmids or DNA samples (Table 1) by real-time TaqMan PCRs assays on a LightCycler® 480 (LC480) (Roche Applied Science, Germany). Real-time PCR assays were performed with LightCycler® 480 Probe Master Mix 1× (Roche Applied Science ...
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bioRxiv - Immunology 2019Quote: ... assay (dynamic range 20-10m) prior to 2010 and using the COBAS Ampliprep/COBAS TaqMan HIV-1 Test version 2.0 by Roche (CAP/CTM v2.0) (dynamic range 20-10m subsequent to 2010 ...
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bioRxiv - Developmental Biology 2019Quote: ... These steps were followed by an incubation of the embryos in anti-digoxigenin-POD (peroxidase) Fab fragments (Roche, 1:50 in TNB). The riboprobe signals were finally detected using fluorescein-tyramide (FITC ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were boiled at 95°C for 5 minutes and diluted 1:1000 in dilution buffer provided in ATP luminescence kit HSII (Roche, Cat# 11699709001). The further assay was performed as instructed by the kit’s manual in Luminometer (Promega GloMax96 Microplate Luminometer) ...
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bioRxiv - Microbiology 2019Quote: HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed ...
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bioRxiv - Physiology 2019Quote: Quantitative real time PCR (qPCR) was performed with primer sets (Supplemental Table 1) designed using Roche Universal Probe Library Assay Design Center (Roche Diagnostics, UK) in conjunction with corresponding FAM-labelled probes ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed twice in 500 µl SPC buffer (1 M Sorbitol, 20 mM PIPES pH 6.3, 0.1 mM CaCl2, with Roche cOmplete protease inhibitors (Sigma)) and spun at 2k RPM for 3 minutes at 4°C between washes ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mio cells were lysed in 100 μl Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, 1x Roche cOmplete protease inhibitors) and sonicated in a microtube (Covaris ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of immunoprecipitated RNA was used for quantification of mRNA expression levels by RT-qPCR as described in the section ‘Quantitative PCR’ except that DNase treatment was omitted and 1 μg yeast RNA (Roche, Basel, Switzerland) was added in the cDNA reaction.
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bioRxiv - Cell Biology 2020Quote: ... Mip1α and housekeeping gene β-actin was evaluated by quantitative RT-PCR using the LightCycler 480 SYBR Green 1 Master kit and LightCycler 480 II (Roche, Indianapolis, IN) and oligos ...
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bioRxiv - Microbiology 2020Quote: ... Treatment of organs was as followed: PBS supplemented with protease inhibitor (1 tablet cOmplete protease inhibitor, Roche in 35 mL of PBS) was added to the frozen organs (volume depending on the weight of the organ ...
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bioRxiv - Pathology 2020Quote: ... Microwave decloaking was performed in 10 mM sodium citrate (pH 6.0) and non-specific sites were blocked in TBSTw containing 1% Blocking Reagent (Roche Diagnostics, Indianapolis, IN), 5% normal goat or donkey sera ...
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bioRxiv - Cancer Biology 2020Quote: ... An overnight blunt ligation was induced for the 5’ of exon 1 and the 3’ of exon 3 (5 U T4 DNA ligase (Roche, Basel, Switzerland)) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 50μl MTT (sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate) labeling reagent (Roche Life Science, USA) to each well ...
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bioRxiv - Biochemistry 2019Quote: Cultured cells were lysed in HEPES-buffered saline (HBS) with 1% NP-40 supplemented with protease inhibitor cocktail (complete protease inhibitor cocktail tablets; Roche Applied Science) on ice for 20 mins ...
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bioRxiv - Microbiology 2020Quote: ... Samples were incubated alone or with ipICP4/pICP4 or BSA for 1 h at 4°C and subsequently fluorescence intensity was measured in a LightCycler II (Roche, Milan, Italy) by observing 6-carboxyfluorescein (6-FAM ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 µg RNA was used for cDNA synthesis with oligo(dT)12-18 using Transcriptor first-strand synthesis kit (Roche, Basel, Switzerland). Transcript levels were measured by qRT-PCR using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Biophysics 2019Quote: ... or for confocal in a alkaline-washed glass-bottomed chamber coated with human plasma fibronectin (25 µg/mL at 4°C overnight in 1/3 X MBS; Roche Molecular Biochemicals) in DFA supplemented with antibiotic/antimycotic (Sigma) ...