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Citations for Roche :
6051 - 6100 of 6383 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... CDMs were washed with PBS and treated for 1 h at 37°C with a buffer of 50 µg/ml DNaseI (Roche, #11284932001), 5 mM MgCl2 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were gradually washed into SSC buffer and then into PBT before overnight incubation with an anti-DIG primary antibody at 1:5000 (Roche #11093274910). Embryos were washed in PBT and then staining buffer before developing in BM Purple staining solution (Roche #11442074001) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each eluate (3 µl) was subjected to PCR amplification in 50-μl reactions containing 1 KAPA HiFi HotStart Uracil+ReadyMix (Kapa Biosystems) and 0.3 μM Dual Index primers of NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Immunology 2024Quote: ... The tissue was weighed and digested with 10μL mg−1 DISC lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% TritonX-100, 10% glycerol, H2O, protease inhibitor cocktail tablet: Roche, Basel, Switzerland). The tissue was lysed with TissueLyser85300 (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed in PBS, incubated in 200ul lysis buffer (50mM Hepes pH 7.4, 150mM NaCl, 1mM MgCl2, 1% NP40, phosSTOP (Roche, Cat# 4906845001), protease inhibitor (Roche ...
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bioRxiv - Biochemistry 2024Quote: Bacterial cell pellets were resuspended in 50 ml of lysis buffer (50 mM Tris-HCl pH 8, 1 mM DTT, 5% glycerol, protease inhibitor cocktail [Roche 1183615300]). Cells in pellets were lysed by sonication on ice with 10 sec pulses of maximum setting followed by 1 min cooling period ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and amplified 6 times (Sets 1 and 2) or 8 times (Set 3) with a KAPA Library Amplification Kit (KAPA Biosystems). The amplification products were cleaned using Agencourt Ampure XP reagent or KAPA Pure Beads ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed in PBS and lysed in lysis buffer (150 mm NaCl, 1% IGEPAL CA-630, 50 mm Tris-HCl, pH 8.0, cOmplete EDTA-free Protease Inhibitor Mixture, Roche Applied Science) supplemented with 20 mm N-ethylmaleimide (NEM) ...
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bioRxiv - Genetics 2024Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (1:5000; anti-DIG-AP, Fab Fragments, Roche, 11093274910). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... samples were covered with the staining mixture at 37°C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... Cryosections were then washed 3 times for 5 minutes in PBT before being incubated with 1:10 TUNEL enzyme buffer (Roche #12156792910) at 37°C in the dark for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Total cell lysates from COS-1 cells 24 hr post transfection were prepared using 300 μl of KAc interaction buffer (Roche Applied Science). GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were dissociated by incubation in DPBS (without Mg2+ or Ca2+) for 20 min at 37°C and then in 1:200 Liberase TH (Roche, Basel, Switzerland) in DPBS for 20 min at 37°C ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Yeast pellets were resuspended in 2 x 500 μl of ice-cold lysis buffer (50mM Hepes, 150mM NaCl, 1% NP-40, 1mM EDTA) containing protease inhibitors (complete Protease Inhibitor Cocktail, Roche Life Sciences), 2 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Neuroscience 2021Quote: ... Beads with cells were washed twice in 1 ml Digitonin Buffer (20 mM HEPES-KOH pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1x Roche cOmplete™; 0.05% digitonin), and resuspended in 50 µl Digitonin Buffer with addition of 2.5 µl CUTANA pAG-MNase (20x stock) ...
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bioRxiv - Microbiology 2021Quote: ChIP followed by qPCR or Illumina sequencing was performed as described58 using the 3F10 rat anti-HA antibody (1:500; Roche no. 11867423001) to immunoprecipitate HA tagged AP2-HS ...
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bioRxiv - Microbiology 2019Quote: ... qPCR was carried out on the cDNA with the LightCycler® 480 SYBR Green 1 Master reaction mix (Roche Diagnostics, Mannheim, Germany), following the manufacturer’s temperature program with 62°C as primer hybridization and elongation temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... MGC803 cells were harvested and used to prepare lysate with cell lysis buffer with 1× Protease Inhibitor Cocktail (REF04693159001, Roche, Mannheim, Germany). The lysate was incubated with Glutathione Sepharose beads (20 μL for one group ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were made from 1∼2 mL packed worms in lysis buffer (25 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% NP-40, 10% glycerol, 1x cocktail of protease inhibitors from Roche (Complete, EDTA free), 40 mM NaF ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% Normal Goat Serum) for 3 h at room temperature and then incubated overnight with alkaline phosphatase-conjugated anti-DIG Fab fragments (Roche, 1: 2,000) at 4°C on a shaking platform ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The final PBMC pellet was taken up in 200 μl lysis buffer (PBS + 1% Triton-X100 + Complete Mini protease inhibitor cocktail tablet (Roche, Mannheim, Germany)) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tissues were homogenized in 1 ml N-PER (brain) or T-PER (other organs) + Complete Mini protease inhibitor cocktail tablet (Roche, Mannheim, Germany) in FastPrep Lysing Matrix D tubes on a FastPrep homogenizer ...
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bioRxiv - Cell Biology 2022Quote: ... minced into ∼1-mm pieces and sequentially digested twice for 30-min in endotoxin-free collagenase (Liberase; Roche Diagnostics GmbH, Mannheim, Germany) to obtain osteocyte-enriched bone samples ...
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bioRxiv - Genomics 2022Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) supplemented with fresh protease inhibitors (AEBSF, Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Emulsions were snap-frozen in droplets in liquid nitrogen and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL digestion mix (4.8 mg/ml Collagenase IV; Worthington Biochem, and 200 μg/ml DNase I; Roche Diagnostics, in 1x PBS) was added after which the tissue was incubated at 37°C for 45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and amplified using a reporter specific forward primer with illumina sequencing adaptors and a reverse primer binding the partial illumina read 1 sequence with the remaining sequencing adaptors using Kapa HiFi HotStart DNA Polymerase (Kapa Biosystems, #KK2601) using 18X cycles for GFP and 23X for Firefly reporter ...
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bioRxiv - Molecular Biology 2019Quote: ... 13 µL of 1:25 diluted cDNA was added to 16.25 µL KAPA 2x SYBR Fast Master Mix (KAPA Biosystems, Wilmington, MA) and 3.25 µL primer sets (Table 2.3 ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μl of TruSeq PCR primer cocktail (50 μM) and 20 μl of KAPA HiFi HotStart ReadyMix (KAPA Biosystems, cat. no. KK2601). USER digestion was performed at 37 °C for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested by centrifugation and lysed by passing them twice through a French press in lysis buffer (1xPBS, 100mM NaCl, 1mM DTT, and complete EDTA-free protease inhibitor tablets (1 tablet/50mL Buffer) (Roche, Basel, Switzerland)) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cells in 6-well plates were lysed with RIPA lysis buffer containing 1mM PMSF and 1% phosphatase inhibitor cocktail (PhosSTOP, Roche group, Swiss). The supernatant was collected after centrifuging at 14,000 rpm for 15 min at 4℃ ...
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bioRxiv - Cell Biology 2019Quote: ... dissolved in 1 ml of ice cold lysis buffer (20 mM Tris base pH 7.5, 80 mM NaCl,1 mM DTT) containing a protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany). The solution was centrifuged for 10 min at 4 °C and 1600 g ...
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bioRxiv - Molecular Biology 2019Quote: ... RT-qPCR was performed with 1 µL of the cDNA synthesis reaction using Kapa SYBR FAST qPCR Master Mix (KapaBiosystems, Roche, Basel, Switzerland) on a Bio-Rad CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2019Quote: Mature cysts were washed in PBS and suspended in lysis buffer (10 mM HEPES, 25 mM KCl, 1 mM CaCl2, 10 mM MgCl2, 2% CHAPS, and 1X Roche protease inhibitor) (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... samples were boiled for 10 min in 1 mL of TE buffer and were loaded on a nylon membrane (Roche, Basel, Switzerland) using a Minislot 30 apparatus (Immunetics ...
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bioRxiv - Neuroscience 2019Quote: ... 30μm thaw-mounted sections were hybridized overnight at 62°C with a DIG-labelled riboprobe for TVAG or HM3 (approximately 1:500; Roche, Cat. 11277073910) and then incubated in Blocking solution (600µl MABT ...
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bioRxiv - Neuroscience 2019Quote: ... Lysis was on ice in complete hypotonic buffer plus 1mM DTT and 1 complete EDTA-free proteinase inhibitor cocktail tablet (Roche Applied Science) per 12ml ...
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bioRxiv - Pathology 2019Quote: ... and plasma HIV-1 RNA levels were determined in HIV-1-infected persons at the Makerere University-John Hopkins University laboratory using standard cell sorting techniques and the Amplicor HIV-1 monitor test (Roche, version 1.5), respectively ...
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bioRxiv - Physiology 2019Quote: ... and the atria were removed before homogenization of the ventricles in 1.5 mL lysis buffer (50 mM HEPES, 150 mM NaCl, 1 mM EGTA, 10% glycerol, 1.5 mM MgCl2, and Complete® protease inhibitor cocktail from Roche, Basel, Switzerland) using a Polytron ...
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bioRxiv - Molecular Biology 2019Quote: Measurement of mitochondrial steady-state ATP levels was carried out in cells incubated with 5 mM 2-deoxy-D-glucose/1 mM pyruvate and no carbon source using the luminometric luciferin-luciferase-based method (CLS II, Roche Applied Science) as described in [35] ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were then stained overnight at 4°C with pre-absorbed AP-conjugated sheep anti-DIG antibody (1:1000, Roche, cat. 11093274910) in PBS containing 0.1% Triton X-100 and 20% horse serum ...
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bioRxiv - Neuroscience 2020Quote: ... 1996) on 16 µm cryosections at post-natal day 3 (P3) using anti-digoxigenin antibody (Sheep polyclonal, 1:1000, Roche, 11093274910, RRID:AB_514497). Two days exposure to alkaline phosphatase (AP ...
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bioRxiv - Biochemistry 2020Quote: ... The gel piece was vacuum dried and treated with trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche sequencing grade) at 37°C for 16 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Total RNA of 1 μg from each sample was reverse transcribed with random hexamers using Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and the presence of enzymes were confirmed by immunoblot using the anti-His6 antibody conjugated to peroxidase (BMG–His-1 monoclonal antibody) (Roche, Mannheim, Germany).
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bioRxiv - Biophysics 2021Quote: ... Pellets were resuspended in lysis buffer (1 M NaCl, 20 mM Tris, 20 mM imidazole, Roche EDTA-free protease inhibitor, pH 7.5) and lysed by sonication ...
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bioRxiv - Physiology 2021Quote: ... a 6.7 kb DNA fragment starting 1 kb upstream of murine Gnrhr exon 3 was amplified by PCR using the Expand Long Template PCR System (Roche, Basel, Switzerland) from 129SvEv genomic DNA using primers incorporating 5’ XmaI and 3’ NotI restriction enzyme sites (Table 1) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 106 cells were lysed for 15 min on ice in 100 μL pre-chilled lysis buffer (50 mM Tris, pH 8, 150 mM NaCl, 1% Triton-X100, 1x cOmplete EDTA-free protease inhibitor cocktail [Roche, Basel, Switzerland]). Lysates were pelleted at 20,000 x g for 15 min at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...