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Citations for Roche :
6051 - 6100 of 7579 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The membrane was probed using the primary antibodies mAb rat α-HA (1:2,000, Roche Diagnostics #11867423001) or mAb mouse α-PfGAPDH (1:20,000 ...
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bioRxiv - Genetics 2024Quote: ... The dried pellets were resuspended in 200 µl of lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM EDTA, 2.75 mM DTT, 1.1 mM PMSF, Roche protease inhibitor at 2x working concentration (Roche 04693132001)) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 30 min at 37 °C with mouse anti-BrdU (11296736, Roche. 1/10) then washed before incubation at room temperature for 3 h with the primary antibody anti-myosin heavy chain (MF20 ...
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bioRxiv - Microbiology 2024Quote: ... 1% triton 100X) supplemented with 0.1 mg/mL lysozyme and complete EDTA-free Protease Inhibitor Cocktail (Roche) for 1 hour on ice ...
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bioRxiv - Plant Biology 2024Quote: ... MAZ-GFP and MAZK141N-GFP accumulation blots were probed with mouse anti-GFP (1:5,000, Roche 1814460001) and goat anti-mouse-HRP (1:10,000 ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.5) supplemented with 1 mM PMSF and cOmplete protease inhibitor cocktail (EDTA-free) (Roche, cat. # 11836170001). One gram of glass beads with a diameter of 0.5 mm (YASUI KIKAI ...
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bioRxiv - Neuroscience 2024Quote: ... and 100,000 worms were collected in 1 ml of RIPA buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ml TLES buffer (TLE + 0.1% (w/v) SDS) with 100 μg/ml proteinase K (Roche, #03115879001) was added and the suspension was incubated at 37°C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... EBs were dissociated into single cells by treatment with 1 mg/ml Dispase II (Roche, 04942 078001) and 2.5 mg/ml collagenase I (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was incubated with a sheep anti-digoxigenin antibody diluted 1:200 (Roche Diagnostics, Basel, Switzerland) conjugated to the peroxidase enzyme ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM PMSF and 1X solution of Complete Mini Protease Inhibitor Cocktail (Roche Applied Science, Mannheim, Germany)] ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were minimally incubated at 65°C for 1 minute during the fragmentation/priming step (Roche, 08098093702). Due to low starting concentrations ...
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bioRxiv - Biochemistry 2024Quote: ... following by incubation with a 1:3,000 dilution primary antibody (anti-GFP from mouse lgG; Roche 11814460001) with 10 mL 5% milk in TBST overnight at 4°C or 1 hour at room temperature.
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bioRxiv - Systems Biology 2024Quote: ... 44 μg ml–1 phenylmethylsulfonyl fluoride) supplemented with Complete Mini protease inhibitor mixture tablets (Roche Applied Science). Total protein was quantified using the bicinchoninic acid assay (Pierce) ...
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bioRxiv - Immunology 2024Quote: ... of the mitochondrial succinate tetrazolium reductase enzyme in a commercial cytotoxic assay kit (CytoSkan WST-1, Roche).
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bioRxiv - Immunology 2024Quote: Lungs were chopped into 1 mm3 fragments and digested with 400 MandlU/ml collagenase D (Roche, Switzerland) and 10 µg/ml DNase I (Roche ...
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bioRxiv - Immunology 2024Quote: ... in a shaking incubator before digestion in 10%FCS/RPMI1640 with 0.625mg ml−1 collagenase D (Roche), 0.425mg ml−1 collagenase V (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% NP-40) supplemented with protease inhibitor cocktail (cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, Cat# 05892791001) was added ...
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bioRxiv - Immunology 2024Quote: ... cOmplete mini EDTA-free protease inhibitor cocktail was added to the supernatants at 1 mg/mL (Roche).
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bioRxiv - Molecular Biology 2024Quote: ... 150 mM KCl, 0.1% NP-40, 0.2 mM PMSF, 0.2mM benzamidine, 1× EDTA-free protease inhibitor cocktail [Roche]). Following clarification by two rounds of centrifugation ...
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bioRxiv - Neuroscience 2024Quote: IPSC-derived dopaminergic neurons were lysed in 1% SDS supplemented with protease inhibitors (Roche, cat no. 11697498001) and immediately boiled at 95°C for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed twice more in PBS and stained with 1 μg/mL DAPI (Roche, 10236276001) in PBS for 5 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... intestinal pieces were digested in 5% FBS medium (RPMI) supplemented with 1 mg/ml collagenase D (Roche) and 0.5 mg/ml DNase I (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... The dissected tumor samples were placed in RPMI with 1.5 U mL-1 Liberase TM (Roche 5401119001), 0.2 mg mL-1 DNase I (Worthington Biochemical LS002006 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were scraped in cold Dulbecco’s PBS buffer (1 mL) containing mini EDTA-free protease inhibitor (Roche) and centrifuged for 1 minute at 13,000 rpm at 4 °C to form a cell pellet ...
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bioRxiv - Neuroscience 2024Quote: ... 5 mM Tris–HCl pH 7.4) supplemented with 1% TX-100 and cOmplete protease inhibitor cocktail (Roche) was loaded in reducing and denaturing conditions on NuPAGE (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries (mRNA-seq) were constructed using 1 μg of material according to KAPA mRNA Hyperprep (Kapa Biosystems) procedure with KAPA Unique Dual Indexes 96 Plate (KAPA Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA (1 μl) was mixed with LightCycler 480 SYBR Green I Master (5 μl, Roche, Basel, Switzerland) and relevant primers (4 μl ...
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bioRxiv - Genetics 2024Quote: ... and resuspended in 100 µL Wash Buffer (1 mL 1M HEPES pH 7.5 [ThermoFisher, J60712.AP], 1.5 mL 5M NaCl, 12.5 µL 2M spermidine, 1 Roche c0mplete Protease Inhibitor EDTA-free tablet with ddH2O to 50 mL) ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were washed and incubated with an anti-DIG antibody coupled to alkaline phosphatase (Roche, 1:3,000). Staining was performed with BM-Purple (Roche).
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bioRxiv - Cancer Biology 2024Quote: ... Specific hybridization was visualized by incubating slides with anti-Digoxigenin-Alkaline phosphatase antibody (1:2000) (#11093274910, Roche) and BM purple AP substrate (#11442074001 ...
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bioRxiv - Cell Biology 2024Quote: ... 1% Triton X-100) supplemented with protease inhibitors (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, Roche) using glass beads (0.5 mm Glass Beads ...
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bioRxiv - Cell Biology 2024Quote: ... 150 mM NaCl and 1 mM EDTA) containing protease inhibitor (PI) cocktail and PhosSTOP™ (04906837001, Roche). The cell lysates were centrifuged at 15000 g for 10 minutes to remove insoluble debris ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche). After sample denaturation and electrophoresis ...
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bioRxiv - Developmental Biology 2024Quote: Pellets were thawed by adding 800 μL ice-cold lysis buffer (50 mM HEPES pH 7.5, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1x Roche cOmplete ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.65% Nonidet P-40 and 1 mM phenylmethylsulfonyl fluoride) supplemented with proteinase inhibitor mixture (cOmpleteTM EDTA-free; Roche). The nuclei were harvested by scraping into a 1.5-ml microcentrifuge tube and collected by centrifugation at 500 × g for 5 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Cell suspension (1 million cells/ml) was mixed with 6x SDS loading buffer and 10 mM DTT (Roche) and incubated at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with the anti-HA high affinity antibody (3F10) (1:500 dilution, Roche – cat. 11867423001), followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... harvested cells were incubated with 25 μg/ml digitonin in buffer H (20 mM Hepes-KOH, pH 7.4, 0.25 M sucrose, 1 mM DTT, complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated at room-temperature with a 1:500 dilution of anti-Dioxigenin-AP Fab fragments (Roche, #11093274910) in 1% BSA/0.3 % Triton-X100/TBS for 1hour ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from asynchronous RPE-1 cells using the High Pure RNA Isolation kit (Roche, 11828665001) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in 10 μM BrdU:BrdC (3:1) for 16–20 h before incubation with 0.1 μg/mL colcemid (Roche) for 2-3 h ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed in ice cold RIPA buffer (50mM Tris HCL, 150mM NaCl, 0.1% SDS, 0.5% DOC, 1%NP-40, EDTA-free protease inhibitor (Roche), phosphatase inhibitors (Halt phosphatase ...
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bioRxiv - Genetics 2021Quote: ... livers were resuspended in a total volume of 4.687 ml of XL Buffer (50 mM Hepes, pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1X Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Genetics 2021Quote: ... Harvested cells in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated in alkaline phosphatase-conjugated anti-DIG (Digoxygenin) antibody Fab fragments (1:5000; Roche, catalog #12486523) at 1:5000 in the blocking buffer and incubated at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... washed with 500 μL buffer MB#1 (10 mM Tris-HCl, pH 7.5, 50 nM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche complete EDTA-free (Roche diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed with a LightCycler® 480 SYBR Green 1 Master reaction mix (Roche Diagnostics, Mannheim, Germany), following the manufacturer’s temperature program with 60°C for the primer hybridization and elongation temperature.
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EDTA, 300 mM NaCl, 10 mM Tris.HCl [pH 8.0], 1 mM EDTA, 1x Complete protease inhibitor [Roche] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Rap1GAP- RlucII/rGFP-CAAX + Gαi2 and D2 or PDZ-RhoGEF-RlucII/rGFP-CAAX + Gα13 and TPαR) using X-tremeGENE 9 DNA transfection reagent (3:1 reagent/DNA ratio; Roche) diluted in OptiMEM (Gibco ...