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Citations for Roche :
6301 - 6350 of 7579 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... 1% Triton X-100, 0.1% sodium deoxycholate, 1mM EDTA, 5mM MgCl2, 1mM DTT, 0.5mM PMSF, 10mM NaF, Roche PhoSTOP ...
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bioRxiv - Plant Biology 2024Quote: ... 1mM DTT and 20 mM imidazole supplemented with 1 tablet of cOmplete™ EDTA-free protease inhibitor (Roche) per every 50 ml ...
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bioRxiv - Plant Biology 2024Quote: ... Tissues were ground with mortor and pestle and resuspended in 30 mL extraction buffer 1 (10 mM Tris-HCl, pH 8.0, 5 mM βmercaptoethanol, 0.4 M sucrose, protease inhibitor cocktail (cOmplete, Roche), vortexed ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were incubated in primary antibody diluted in blocking buffer [rat anti-HA HRP 1:500 (Roche 12013819001), mouse anti-tubulin 1:1000 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... washed with cold PBS and then lysed for 4 hours on rotation at 4°C in TNEN buffer (50 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.1% NP-40, Protease inhibitors (Roche) and phosphatase inhibitors (Sigma ...
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bioRxiv - Immunology 2024Quote: ... followed by a subsequent incubation at 95 °C in pre-formulated Cell Conditioning 1 solution (Roche Diagnostics, Canada). Following this ...
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bioRxiv - Plant Biology 2024Quote: ... supplemented with 1 tablet of protease inhibitor cocktail per 10 ml buffer (cOmplete™ Proteasehemmer-Cocktail, ©Roche) with an ultrasonic homogenizer (Hielscher Ultrasonics UP200St ...
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bioRxiv - Biochemistry 2024Quote: ... HA- and GFP-tagged proteins were detected using horseradish peroxidase-conjugated monoclonal anti-HA (Roche, 3F10, 1:5,000) and anti-GFP (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were stopped by adding 0.5 μl ethylenediaminetetraacetic (0.5 M EDTA) and 1 μl Proteinase K (19 mg/ml, Roche), and incubated at 50°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and 72°C for 1 min) All PCR was performed using KAPA HiFi HotStart ReadyMix (Kapa Biosystems; KK2602). The PCR products were isolated by QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Pathology 2024Quote: ... Pre-cleared lysates (containing 1 mg of protein) were then incubated with mouse monoclonal anti-GFP antibodies (Roche) coupled to G-protein beads for 3 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were washed two times with 300 μL of 1%BSA antibody buffer (20mM HEPES– KOH, pH 7.5, 150 mM KCl,0.5 mM Spermidine, 1X Roche cOmplete Mini EDTA-free Protease Inhibitor ...
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bioRxiv - Microbiology 2024Quote: ... and its activity was measured by addition of 1 mM chlorophenol red-β-D-galactopyranoside (Roche Diagnostics, GmbH) in 100 mM HEPES pH 8 (Dutscher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Pull-down was performed on the cell lysates by adding 1 µg of α-HA (Sigma Roche 12CA5) or mouse α-IgG (Santa Cruz Biotechnology sc-2025 ...
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bioRxiv - Cell Biology 2024Quote: ... Bound protein was eluted by heating immunoglobulin G beads with 35 μl of denaturing urea SDS-loading dye (1% SDS, 8 M urea, 10 mM Tris, pH 6.8, 10 mM EDTA, 0.01% bromophenol blue, 1x Roche Complete inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... NaCl 140 mM, EDTA 1 mM, Glycérol 10 %, NP40 0.5 %, AEBSF 1mM, Protease inhibitor cocktail Complete 1x, Roche) and resuspended in 30 µl of Laemmli buffer (1 x) ...
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bioRxiv - Systems Biology 2024Quote: ... 1 mL of lysis buffer (25 mM Ammonia Bicarbonate (AmBic) with protease inhibitors (Roche complete protease inhibitor tablets)) was used to resuspend the thawed pellets and the sample was transferred to a 2 mL screw cap vial for bead beading in 2 mL Precellys vials prefilled with 0.5mm glass beads ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was performed on LightCycler© 480 thermocycler using the SYBR Green 1 Master kit (Roche, Switzerland). Primers were used at 200 nM ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) supplemented with complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche) and 1 mg/ml lysozyme (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... The remaining 90% of each sample was immunoprecipitated with 1:1000 anti-HA antibody (0.5 mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
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bioRxiv - Developmental Biology 2024Quote: ... harvested and lysed 14 h later in 300 µl binding buffer (20 mM HEPES pH 7.6, 150 mM MgCl2, 10% glycerol, 0.05% NP-40, 1 mM DTT, ROCHE cOmpleteTM ULTRA-tablet Mini protease inhibitor) ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended in 150 µl of lysis buffer (PBS 1X, 1% protease inhibitor cocktail EDTA-free, Roche) and lysed by sonication (amplitude 70% ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were then probed with either Horse Radish Peroxidase (HRP)-conjugated 3F10 anti-HA (1:2000, Roche), HRP-conjugated FLAG M2 (1:5000 ...
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bioRxiv - Pathology 2024Quote: ... Slides were incubated for 30 minutes with 1 unit of DNase I (Roche, Cas. No. 9003-98-9) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Nuclei were centrifuged at 4°C at 1,300 x g for 4 min and then resuspended in 1 mL of Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, supplemented with Roche complete EDTA-free protease inhibitor tablet) ...
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bioRxiv - Pathology 2024Quote: ... by directly assessing 1 drop of whole blood using an Accu Chek Aviva glucose meter (Roche Diagnostics, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Crosslinked tissue was resuspended in 1.5 mL lysis buffer (1X PBS, 1% NP-40, 0.5% NaDOC and 0.1% SDS with cOmplete protease inhibitors (Roche)) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% (v/v) Triton X-100) to leave only matrix before treatment with 10μg/ml DNAse 1 (Roche) to cleave phosphodiester linkages in the DNA backbone ...
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bioRxiv - Immunology 2024Quote: ... were isolated by incubating specific nitrocellulose membrane fragments with PK buffer (100 mM This-Cl pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.2 % SDS and 20 U of proteinase K [Roche]). After 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The beads-bound nuclei were resuspended in 200 µl of Tween-Wash buffer (0.1% Tween-20, 20 mM HEPES-potassium hydroxide, pH 7.5, 150 mM sodium chloride, 0.5 mM spermidine, 1 Roche cOmplete EDTA-free tablet per 50 ml buffer and 0.1% BSA ...
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bioRxiv - Genetics 2024Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
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bioRxiv - Genetics 2024Quote: ... resuspended in 900 μl shearing buffer (0.1% SDS, 10 mM Tris-HCl pH 8.0, 1 mM EDTA) supplemented with protease inhibitor (Roche), split into two~500 μl aliquots and sonicated to fragment sizes ranging 500–1000 bp with a Covaris system (E220 Focused-ultrasonicator ...
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bioRxiv - Genomics 2024Quote: ... and 1 μl of 10 mM of each primer using LightCycler® 96 System (Roche Diagnostics, Mannheim, Germany). The thermal cycler was programmed as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μl of forward and reverse primers (each 10 μM) and 10 μl of SYBR Green I (Roche) were combined to a total volume of 20 μl ...
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bioRxiv - Cell Biology 2024Quote: ... 10 mM KCl, 1.5 mM MgCl2, 0.34 M sucrose, 10% glycerol, 1 mM dithiothreitol [DTT], a protease inhibitor cocktail [Roche]). Triton X-100 (0.1% ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended for lysis in RIPA buffer (50 mM Tris pH 7.6, 150mM NaCl, 1% Triton X-100, 0.1% SDS) with protease (04693159001, Roche) and phosphatase inhibitors (524625 ...
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bioRxiv - Developmental Biology 2024Quote: ... Localization of HA tagged proteins were analyzed with an anti-HA tag antibody (Roche, clone 3F10, 1 : 500) and Alexa Fluor coupled secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cartilage was then digested for 30 minutes at 37°C in 1 mg/ml pronase (Roche, Basel, Switzerland) and then overnight at 37°C in 1 mg/ml collagenase P (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellets were then resuspended in 400 uL Lysis buffer (1% SDS, 50 mM Tris Hcl pH 8.0 20 mM EDTA, protease inhibitors (Roche) and incubated on ice for 10 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... in liquid nitrogen in a 1.5-ml Eppendorf tube and resuspended in ice-cold lysis buffer (50 mM Tris pH 7.6, 10 mM EDTA, 150 mM NaCl, 1% Triton, supplemented with complete protease inhibitor, Roche Ref ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was lysed with 20 ml of Eps8L2 Lysis Buffer (10 mM Tris.HCLpH8, 150 mM NaCl, 1 mMEDTA, lysozyme 0.1 mg/ml, Pefabloc® (Roche) 0.5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in lysis buffer supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... pH 8.0, 100 mM NaCl, 5mM MgCl2, 1% Triton X-100, 1mM PMSF, and complete protease inhibitor cocktail; Roche) and lysed twice with high pressure (1200 psi ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 mL ddH2O and resuspended in 100 μL lysis buffer (50 mM Tris, pH 7.4, 50 mM dithiotreitol, 1 mM EDTA, cOmplete EDTA-free protease inhibitor cocktail (Roche) and Phosstop (Roche) ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The lysis was performed in RIPA buffer (1% SDS, 10 mM Tris pH 8.0, containing protease and phosphatase inhibitor cocktail tablets (Roche)) ...
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bioRxiv - Developmental Biology 2021Quote: ... Wing discs of L3 larvae were hybridized with probes overnight at 56 °C using standard procedures and visualized using anti-Dig-AP (1:1,000; Roche). Primers used for generating PCR templates are listed in Key Resources Table.
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bioRxiv - Immunology 2021Quote: ... Tissue homogenates were resuspended in 1x lysis buffer (1% NP-40, 100mM Tris, pH 8.0, and 150mM NaCl) containing a protease inhibitor mixture (Roche) and shaken 60 mins at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide, 1 mg/ml tRNA from Roche, 5 mM EDTA from Sigma and 50 μg/ml Heparin from Sigma ...
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bioRxiv - Biophysics 2021Quote: ... The cell pellet was resuspended in 1 ml of polysome lysis buffer supplemented with EDTA-free protease inhibitors (Roche) and was grounded in liquid nitrogen using an RNase-free mortar and pestle ...
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bioRxiv - Developmental Biology 2021Quote: ... Eye discs were then removed from the mouth hooks and blocked for 1 hour in 1X PBX:Western Blocking Reagent (Roche) at room temperature with nutation ...