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Citations for Roche :
6001 - 6050 of 7615 citations for 7 Methylimidazo 1 2 a pyridine 3 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with the anti-HA high affinity antibody (3F10) (1:500 dilution, Roche – cat. 11867423001), followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... harvested cells were incubated with 25 μg/ml digitonin in buffer H (20 mM Hepes-KOH, pH 7.4, 0.25 M sucrose, 1 mM DTT, complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated at room-temperature with a 1:500 dilution of anti-Dioxigenin-AP Fab fragments (Roche, #11093274910) in 1% BSA/0.3 % Triton-X100/TBS for 1hour ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from asynchronous RPE-1 cells using the High Pure RNA Isolation kit (Roche, 11828665001) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed in ice cold RIPA buffer (50mM Tris HCL, 150mM NaCl, 0.1% SDS, 0.5% DOC, 1%NP-40, EDTA-free protease inhibitor (Roche), phosphatase inhibitors (Halt phosphatase ...
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bioRxiv - Genetics 2021Quote: ... livers were resuspended in a total volume of 4.687 ml of XL Buffer (50 mM Hepes, pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1X Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Genetics 2021Quote: ... Harvested cells in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated in alkaline phosphatase-conjugated anti-DIG (Digoxygenin) antibody Fab fragments (1:5000; Roche, catalog #12486523) at 1:5000 in the blocking buffer and incubated at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... washed with 500 μL buffer MB#1 (10 mM Tris-HCl, pH 7.5, 50 nM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche complete EDTA-free (Roche diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed with a LightCycler® 480 SYBR Green 1 Master reaction mix (Roche Diagnostics, Mannheim, Germany), following the manufacturer’s temperature program with 60°C for the primer hybridization and elongation temperature.
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EDTA, 300 mM NaCl, 10 mM Tris.HCl [pH 8.0], 1 mM EDTA, 1x Complete protease inhibitor [Roche] ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM MgCl2, 10 % v/v glycerol, 140 mM NaCl, 0.1 mM EDTA, 0.5 % Tween20, 1 mM DTT and Roche cOmplete-Ultra EDTA free protease inhibitor) ...
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bioRxiv - Molecular Biology 2019Quote: ... with addition of 1 mM phenylmethylsulfonyl fluoride (PMSF) and cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche). His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... participants were given either a total of 225mg of L-DOPA (Madopar, Roche, Levodopa/Benserazid, 4:1 ratio) or a placebo (P-Tabletten white 8mm Lichtenstein ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) and sonicated for 90 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% NP40 and 10 mM Tris (pH 7.5)) supplemented with cOmplete EDTA-free protease inhibitor (Roche, Basel, Switzerland). Samples were incubated on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 350 μl of lysis buffer (20 mM Tris pH 7.5, 0.5 M o NaCl, 1 mM EDTA, 1X protease inhibitor cocktail [Complete; Roche]) and 200 μl of glass beads (425-600 μm ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed three times on the magnet in 1 mL buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Roche complete protease inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Nonidet-P40, 0.2 mM TCEP, and Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Immunology 2019Quote: ... 50 mM KCl and 0.45% Tergitol at pH 8.0) that was freshly supplemented with 1 μg/mL proteinase K (Roche). After deactivating proteinase K by incubating for 15min at 95 °C ...
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bioRxiv - Immunology 2019Quote: ... pH 7.4, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40 and 0.5% SDS supplemented with protease inhibitor [Roche]) and centrifuged at 12,000 g at 4°C for 10 min ...
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bioRxiv - Developmental Biology 2019Quote: ... Supernatant containing cytosol protein was mixed with 1% SDS solution supplemented with proteinase inhibitor (PI) cocktail (Roche, 5892970001), and then was used for western blotting ...
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bioRxiv - Immunology 2019Quote: ... 1% Pen/Strep) containing 0.25 mg/mL Liberase TL and 20 U/mL DNase I (both from Roche), stirring at 37°C for 1h ...
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bioRxiv - Physiology 2020Quote: ... The sections were incubated with sheep anti-DIG antibody (1:200, Roche Applied Science; cat. no 1333 089), biotinylated donkey anti-sheep antibody (1:200 ...
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bioRxiv - Genetics 2019Quote: ... with cDNA then being generated from 1 μg of RNA utilizing the First Strand Reverse Transcriptase kit (Roche). Quantitative PCR was utilized to assess target gene expression using the KAPA SYBR Fast QPCR Master Mix ...
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bioRxiv - Molecular Biology 2019Quote: ... resuspended in modified RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Igepal, 0,25% Na-Deoxycholate, protease inhibitor cocktail without EDTA-Roche), homogenized through a 23G needle on ice ...
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bioRxiv - Evolutionary Biology 2019Quote: ... HIV-1 genomic RNA was extracted from supernatants containing virions using High Pure Viral RNA kit (Roche Inc.). SuperScript III and a primer downstream of the randomized regions were used to reverse transcribe viral RNA to cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... cardiomyocytes were dissociated using DPBS for 20 min at 37 °C followed by 1:200 Liberase TH (Roche) diluted in DPBS for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2019Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10,000; Roche Diagnostics, Germany). The color staining was carried out with chromogen substrates (nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate ...
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bioRxiv - Immunology 2019Quote: ... and each LN was placed in 1 mL of pre-warmed digestion media (0.8 mg/mL dispase, 0.2 mg/mL collagenase P (Roche), 50 µg/mL of DNase I in LN media) ...
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bioRxiv - Zoology 2019Quote: ... After incubation at room temperature for 4 hours in 1:2000 dilution of anti-digoxigenin antibody (Roche #11376623) prepared in TBST containing 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or p89.6) and an envelope deficient HIV-1 backbone vector (pSG3Δenv) using XtremeGENE HP DNA Transfection Reagent (Roche). After 24 hours of incubation at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: pLKO.1 lentiviral construct along with packaging vector ΔVPR and VSVG plasmids were transfected using Fugene-6 (Roche) or PEI (POLYSCIENCES 23966-2 ...
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bioRxiv - Neuroscience 2019Quote: ... then immersed in 0.1 M maleate buffer (pH 7.5) for 10 minutes before blocking in 1% Blocking Reagent (Roche) for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1% SDS) and subsequently resuspended in 900 µL shearing buffer with added 1× protease inhibitors complete mini (Roche). Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode ...
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bioRxiv - Genomics 2021Quote: ... Cells were lysed by the addition of 1000 μL lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8.0, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice to generate an average DNA fragment size of ∼250 bp ...
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bioRxiv - Developmental Biology 2021Quote: ... Fixed embryos were placed into hybridization buffer (50% formamide, 5×SSC, 1 mg/ml yeast tRNA (Roche, 10109223001), 100 μg/ml heparin (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Whole cell extracts were prepared in HEPES whole cell lysis buffer (20 mM HEPES, pH 7.4, 1% TX-100,1x phosphoSTOP complete inhibition cocktail tablets [Roche] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the samples were washed in 0.1M glycine pH 2.0 and then incubated overnight with anti-fluorescein (1:250) (Roche). Samples were then washed in TNT ...
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bioRxiv - Neuroscience 2021Quote: ... the reaction product was developed using NBT/BCIP solution diluted in pre-staining buffer (1:100; Roche, Switzerland) overnight at RT ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:1500 in blocking solution; Roche, Switzerland) overnight at RT ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM EDTA) supplemented with protease inhibitors (complete mini EDTA-free, Roche, 1 tablet / 50 mL lysis buffer). The lysed neurons were mixed with 4X Laemmli sample buffer and heated at 70°C for 10 minutes (stored at −20°C until use) ...
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bioRxiv - Neuroscience 2020Quote: ... The incubation with the anti-digoxigenin-POD took place afterwards (1/2000 dilution in BSA/TBST, Roche Diagnostics) at room temperature for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... 5 mM MgCl2, 20 mM Tricine-KOH, 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, 1X Roche EDTA-free protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2019Quote: ... First the tissue was mechanically dissociated using a tissue grinder while submerged in 1 ml of TriPure (Roche). 200 µl of chloroform (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... For CHIP-qPCR: qPCR was performed on diluted samples using LightCycler 480 SYBR green 1 master kit (Roche) using primers listed below.
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bioRxiv - Biochemistry 2020Quote: ... the medium was replaced by serum free DMEM supplemented with 1% fatty acid free BSA (Roche Applied Sciences) and a mixture of oleate and palmitate (ratio 2:1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in 450 μl lysis buffer (20 mM Tris pH 7.5, 0.5 mM EDTA pH 8, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 10 mM NEM, Roche cOmplete protease inhibitor catalog # 11836153001) ...
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bioRxiv - Plant Biology 2020Quote: ... 0.5% (w/v) PVP40 and Roche protease inhibitor cocktail (1 tablet per 50 ml of extraction buffer, Roche). After rocking on ice for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... 2 * 107 cells were pelleted and resuspended in 1 ml of lysis buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) including 0.25x protease inhibitor (Roche)) ...