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Citations for Roche :
5951 - 6000 of 6180 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Pellets were resuspended in lysis buffer (1 M NaCl, 20 mM Tris, 20 mM imidazole, Roche EDTA-free protease inhibitor, pH 7.5) and lysed by sonication ...
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bioRxiv - Immunology 2020Quote: ... The cDNA samples were diluted 1:10 in water and analyzed in triplicate using a LightCycler 480 detection system (Roche, Meylan, France). PCR conditions were 95°C for 5 min and 45 cycles of 95°C for 15 s ...
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bioRxiv - Cell Biology 2021Quote: ... and finally washed with HK buffer (25mM HEPES pH 7.2, 150mM KCl, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, Complete EDTA free)) ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... followed by addition of 100 μL ChIP Elution Buffer (50 mM Tris-HCl, pH 7.5 @ RT, 10 mM EDTA, 1% SDS) and 20 μL Pronase (Roche, 20 mg/mL) for incubation at 42°C for 2 hours ...
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bioRxiv - Genetics 2020Quote: Cell pellets from THP-1 cells were suspended on ice-cold lysis buffer (PBS containing 2% SDS and complete protease inhibitor cocktail (Roche, catalog 11697498001)) ...
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bioRxiv - Cell Biology 2022Quote: ... and processed in digestion buffer (1 mg/ml of Collagenase D and 80 U/ml DNase I, both from Roche, in DMEM) and a GentleMACS dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... The mRNA abundance for the genes (Supplementary Table 1) of interest was determined by quantitative PCR using Fast Start SYBR Green (Roche, Cat# 4673484001). The data was quantified by the comparative ΔCT method and expressed relative to β-actin mRNA.
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bioRxiv - Immunology 2022Quote: ... These tissues were cut to ∼2mm pieces and incubated for 1h at 37ºC in the enzymatic cocktail containing Colleagenase D (Roche, 1 mg/ml) and DNAse I (Roche ...
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bioRxiv - Genomics 2022Quote: ... nuclei were incubated with streptavidin conjugated secondary antibody diluted 1:100 in Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) for 45 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... Adapter ligation was performed for 1 hour at 20°C using the Kapa Unique-Dual Indexed Adapters diluted to 1.5 μM concentration (Roche Sequencing Solutions Inc.). After ligation ...
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bioRxiv - Microbiology 2022Quote: ... was used at a 1:1000 dilution to detect specific anti–SARS-2 immunoreactivity using the Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... and pIZT-CS2 (1 μg) containing the complete cDNA sequence of BmCPV S2 dsRNA encoding RdRp using Roche-X Gem (Switzerland, Roche, Cat: 6366236001). At 48 h posttransfection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The left lobe minced into small (~1-2 mm) sections and digested using 0.5 mg/mL collagenase D (Roche Diagnostics, Mannheim, Germany) and 50 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellet was suspended in 1 mL of TAP buffer (100 mM NaCl, 20 mM Tris-HCl pH 8, 2.5 mM EDTA, 10 % glycerol, 1 % Triton, 1x of PhosSTOP, 1x Roche protease inhibitor mixture) and given 20 strokes with the Dounce Homogenizer ...
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bioRxiv - Bioengineering 2024Quote: ... were purified over a 1-mL column of anti-protein C affinity matrix according to the manufacturer’s protocol (Roche, cat. no. 11815024001). 1 mM CaCl2 was added to the supernatant ...
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bioRxiv - Cell Biology 2024Quote: ... cells were first incubated 1 hour with Blocking Buffer (10% heat-inactivated Goat Serum, 0.5% Blocking Reagent [Roche] in PBS-0.5% Tween) and then 1 hour with 1.5U/ml αFAM-POD antibody (Roche ...
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bioRxiv - Physiology 2024Quote: Coarsely chopped liver fragments from each mouse were digested in 5 ml liberase-based digestion mix (5 ml of DMEM [Thermo Fisher Scientific, Cat #61965059] + 1 mg Liberase™ Research Grade [Roche, Cat #5401127001] + 0.1 μg DNAse I [Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... or 48 h after seeding in a Triton X-100-based buffer (150 mM NaCl, 20 mM Tris-HCl pH 7.5, 5 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). A linear sucrose density gradient was prepared in 13x51 mm centrifuge tubes (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed by addition of 250 μl lysis buffer (50 mM Tris/HCl, 500 mM NaCl, 2% w/v SDS, add freshly 1 mM DTT, 1x Roche inhibitor cocktail) followed by incubation on ice for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with a solution containing horseradish peroxidase (HRP)- conjugated anti-Dig (at a dilution of 1:500; #11207733910, Roche Applied Science) and goat anti-mCherry (at a dilution of 1:250 ...
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bioRxiv - Biochemistry 2024Quote: ... tubes and homogenized for 1.5 minutes in 50μL of Tris-buffered saline (TBS; Table 1) with 1X protease and 1X phosphatase inhibitor cocktails (Roche, 04693124001 and 04906837001). The homogenates were then probe-sonicated with a Branson Sonifier® SFX150 (Emerson ...
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections were incubated with anti-HA antibody (1:400 dilution) followed by incubation with anti-rabbit-HQ (Roche; Cat#. 760-4815) and anti-HQ HRP (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: Paired-end sequencing libraries for QTL-Seq analysis were prepared using >1 μg of pooled DNA with a KAPA HyperPrep PCR-free kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Exome libraries were prepared from 1 μg of genomic DNA from each analyzed section using the Nimblegen EZ Exome kit V3 (Roche, Nutley, NJ). Paired-end 100 bp sequencing was performed on a HiSeq2500 sequencer (Illumina Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... twice with High Salt Wash Buffer (500 mM NaCl, 0.1 % SDS, 1 % Triton X-100, 2 mM EDTA, 20 mM Tris-HCl 8, 1x Roche protease inhibitor mixture), and twice with TE wash buffer (10 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2024Quote: ... the full-length Treacle was amplified by PCR from cDNA with primer set #4 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained BglII and BamHI sites respectively ...
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bioRxiv - Immunology 2024Quote: ... Tumours were dilacerated using scalpels to obtain <1mm sized pieces and resuspended in R10 supplemented with 1 mg/mL Liberase TL (Roche, Cat. 5401020001) and 10 μg/mL DNase I (Roche ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was isolated by isopropanol precipitation of cells and tumor lysates (lysis buffer was Tris [pH 8] 100 mM, EDTA 5 mM, SDS 0.2%, NaCl 200 mM, and 1 mg/mL proteinase K [Roche Diagnostics, Mannheim, Germany]) and resuspended in Tris/EDTA (TE ...
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bioRxiv - Neuroscience 2023Quote: ... then lysed on ice for 15 minutes in non-denaturing lysis buffer (150 mM KCl, 10 mM MgCl2, 5mM HEPES, 1% IGEPAL) supplemented with protease inhibitors (Roche Complete EDTA-free, Roche, Laval, Canada). The samples containing lysis buffer were then collected and spun down for 5 minutes in pellet the cell debris ...
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bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl, 10% glycerol, 1% IGEPAL [Sigma, St Louis, MO], and cOmplete EDTA-free protease inhibitor cocktail [Roche] [pH 7.4]). Cells were vortexed ...
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bioRxiv - Molecular Biology 2023Quote: ... Viral loads were measured at all study visits using the Roche Cobas Taqman HIV-1 Test v2.0 (Roche Diagnostics, Branchburg, NJ, USA). CD4 T cell counts were also enumerated at all study visits using the 4-colour MultiTEST/Trucount assay (Becton Dickinson ...
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bioRxiv - Molecular Biology 2023Quote: ... were sequenced in the forward direction on 1/8 plate of a 454 Life Sciences Genome Sequencer FLX machine (Roche, Branford, CT) using the Macrogen facilities (South Korea).
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bioRxiv - Developmental Biology 2023Quote: ... The qPCR was performed using PerfectStartTM Green qPCR Super Mix (TransGen Biotech, 1) on a Real-time PCR Detection System (Roche, LightCycle480 II). RPLP0 served as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... Vero/TMPRSS2 cells (JCRB Cell Bank, Cat# JCRB1818) were cultured in DMEM containing 5% FBS and 1 mg/mL G418 (Roche, Basel, Switzerland). VeroE6/TMPRSS2 cells (JCRB Cell Bank ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were lysed in 50 µl of lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 1× Roche Complete protease inhibitors cocktail) by pipetting up and down ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with 12 ml of elution buffer (20 mM Tris, 500 mM NaCl, 6 M guanidine, 600 mM imidazole, 1 mM MgCl2 and 1X Roche protease inhibitor) and exchanged into #1 to #5 dialysis buffers in order to remove the imidazole and the Guanidine ...
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bioRxiv - Genomics 2023Quote: ... 3 million crosslinked cells were resuspended in 1 mL of ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP-40, and 1× Roche protease inhibitors Cat#11697498001) and rotated at 4 °C for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... in 5% non-fat dry milk in TBS/0.1% Tween-20 (TBST) or in 1% Western blocking reagent (WBR) (Roche Applied Science, Germany) in TBS (for β-actin) ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed in immunoprecipitation buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% TritonX-100 supplemented with Roche protease inhibitor cocktail). Lysates were centrifuged (5,000x g ...
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bioRxiv - Cell Biology 2023Quote: ... Livers were collected on ice and incubated in digestion buffer (0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The cell pellet was washed once with 50 mM Tris-HCl (pH 7.0) and resuspended in 1 mL of 50 mM Tris-HCl (pH 7.0) containing protease inhibitors (cOmplete, Roche Applied Science, Mannheim, Germany). The cells were disrupted with glass beads using a FastPrep system (Qbiogene ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested and either lysed using sonication in HED buffer (25 mM HEPES, 10% glycerol, 1 mM DTT, and EDTA-free protease inhibitor (Roche, Basel, Switzerland)) containing 0.1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007, Roche, Bâle, Switzerland) or anti-β-tubulin mouse antibody (Catalogue number T9026 ...
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bioRxiv - Immunology 2023Quote: ... and PLN from non-diabetic 6w– 8w female mice were minced and enzymatically dissociated with 1 mg/mL collagenase D (Roche Applied Science) and 25 U/mL DNase I in DMEM for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were brought to room temperature and washed twice in TNT before being incubated in blocking buffer (TNT+5% sheep serum+1% Roche blocking reagent) for 1 hour ...