Labshake search
Citations for Roche :
5701 - 5750 of 6180 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... and subsequent addition of 50 µl lysis buffer supplemented with 1 mM PMSF / ml buffer and 22 µl / ml buffer protease inhibitor cocktail (Complete, Roche Diagnostics). The cell samples were boiled for 3 minutes ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 10% sheep serum] and incubated with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Cat# 11093274910) in MABT/Block at 4°C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... The HEK 293T cells were mixed with 1 μg of mouse Ano9 cDNA (mAno9-pEGFP-N1) and 3 μl FuGene HD (Roche Diagnostics). The transfected cells were plated onto glass coverslips that were kept in a 35-mm round Petri dish ...
-
bioRxiv - Cancer Biology 2022Quote: ... AML blast cells were incubated in 1 ml of ice-cold 0.1 M Na2CO3 containing complete protease inhibitor (Roche, Penzberg, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
-
bioRxiv - Neuroscience 2021Quote: ... proteinase K treatment was omitted and color development of alkaline phosphatase activity was carried out for 1 h with nitroblue tetrazolium chloride (Roche Diagnostics) and 5-bromo-4-chloro-3-indolyl phosphate (Roche Diagnostics ...
-
bioRxiv - Neuroscience 2019Quote: ... 0.5% Triton X-100, 100 mM NaF, 1 mM ortho-vanadate, 2 mM EDTA, and a protease inhibitor cocktail [Roche 11836153001]). Lysates were vortexed and cleared by centrifugation (15,000 x g for 15 min at 4°C) ...
-
bioRxiv - Molecular Biology 2020Quote: ... nuclei were pelleted by centrifugation at 2500g for 5 minutes and resuspended in 880ul Nuclear Lysis Buffer (50mM Tris HCl, 10mM EDTA, 1% SDS, 1X protease inhibitors (Roche, 04693124001)) ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 M sucrose, 5 mM KCl, 5 mM MgCl2, 0.6% Triton X-100, 0.4 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... The pellet of 100 mL was resuspended in 1 mL cold fractionation buffer (40 mM HEPES, 0.1 mM EDTA, 1x cOmplete™ Protease Inhibitor Cocktail (Roche Sigma)) and the pellet of 1 mL lysed with Bug Buster (pellet sample ...
-
bioRxiv - Neuroscience 2020Quote: ... 1 μM benzyloxycarbonyl (Z)-Leu-Leu-Leucinal (protease inhibitor, synthesized in-house) and Complete EDTA-free Protease Inhibitor (Roche, cat# 05056489001) in 50 mm MES pH 6.5 with or without 10 μM thiorphan (neprilysin-specific inhibitor ...
-
bioRxiv - Cancer Biology 2021Quote: ... and digested either for 1 hour at 37°C in a digestion mix of 3 mg/ml collagenase type A (Roche, 11088793001) and 25 μg/ml DNAse (Invitrogen ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral supernatants were prepared by transfecting 293T cells with GFP- or ABCG-targeting shRNAs encoded in pLKO.1 vectors using X-tremeGENE 360 transfection reagent (Roche, Germany) as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... and tissue homogenization was performed using a Bullet Blender in RIPA buffer supplemented with 1% cOmpleteTM protease inhibitor cocktail (Roche Diagnostics), 1% phosphatase inhibitor cocktail 2 (#P5726 ...
-
bioRxiv - Genetics 2022Quote: ... Samples were suspended in Laemmli sample buffer supplemented with a protease and phosphatase inhibitor cocktail (PIC; 1 mM PMSF, cOmplete™ Protease Inhibitor Cocktail [Roche, Base ...
-
bioRxiv - Pathology 2022Quote: ... labeling probe (359 bp) was synthesized using primers TiPV-Fq and TiPV-NS1-R (Table 1) and DIG-labeling mix reagent (Roche, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed with cold PBS and lysed using buffer A (PBS, 20 mM imidazole, 1% Triton X-100, and a cOmplete® protease inhibitor tablet (Roche). Lysates were clarified for 30 min at 13,000 g at 4°C ...
-
bioRxiv - Plant Biology 2019Quote: ... was performed using gene-specific primers (Supplementary Table 1) and the fluorescent intercalating dye Kapa SYBR Green (Kapa SYBR FAST qPCR Master Mix Universal, Kapa Biosystems) in a BIORAD CFX ConnectTM Real-Time PCR Detection System ...
-
bioRxiv - Molecular Biology 2019Quote: ... Each PCR reaction was composed of the following ingredients: 1 μl 10× FastStart PCR Buffer comprising 20 mM MgCl2 (Roche Diagnostics), 0.2 μl dNTPs (10 mM each) ...
-
bioRxiv - Molecular Biology 2019Quote: Re-amplification of primary WTA products was performed in a reaction volume of 50 μl comprising 5 μl Expand Long Template Buffer 1 (Expand Long Template PCR System, Roche Diagnostics), 6 μl of CP2-15C or CP2-9C primer (2.88 μM ...
-
bioRxiv - Molecular Biology 2019Quote: ... primary or re-amplified WTA products) added to 1 μl Expand Long Template Buffer 2 (Expand Long Template PCR System, Roche Diagnostics), 1 μl dNTPs (10 mM each) ...
-
bioRxiv - Neuroscience 2019Quote: ... DRGs were dissected out from 8-week old adult mice and incubated with collagenase A (1 mg/ml; Roche, Basel, Switzerland) for 90 minutes and then with 1X TrypLE (Life Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... For quantitative-RT PCR analysis 1 µg of total RNA of each sample was reversed-transcribed to cDNA (Transcriptor First Strand cDNA Synthesis kit, Roche 04379012001), cDNA was diluted 1:20 and used as template for amplification reactions ...
-
bioRxiv - Zoology 2019Quote: cDNA (1 μL) from control larvae was amplified using the KAPA HiFi HotStart DNA Polymerase Taq (Kapa Biosystems, Wilmington, MA, USA), insect-HSP70 degenerate primers (from Bernabò et al. ...
-
bioRxiv - Genetics 2019Quote: ... Coverslips were blocked in 3% BSA (wt/vol) and digoxigenin was detected with sheep anti-digoxigenin FITC 1/50 (Roche, 11207741910) followed by rabbit anti–sheep FITC 1/100 (Vector Laboratories ...
-
bioRxiv - Molecular Biology 2020Quote: ... the lysed cells were resuspended in LB2 (10 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, Roche cOmplete protease inhibitors) and incubated at 35 rpm for 5 min followed by centrifugation ...
-
bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 150 mM NaCl, 1% NP-40, 2 mM EGTA, and complete protease and phosphatase inhibitors from Roche, Basel, Switzerland). Cell lysate was cleared by centrifugation ...
-
bioRxiv - Genomics 2019Quote: ... transcripts derived from self-reporting transposons) involved mixing 1 µl cDNA template with 12.5 µl Kapa HiFi HotStart ReadyMix (Kapa Biosystems #KK2601), 0.5 µl 25 µM SMART primer ...
-
Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... sample pellets were resuspended in 200 µL lysis buffer (100 mM triethylammonium bicarbonate buffer (TEAB) with 1% (v/v) sodium deoxycholate (SDC) and 1x protease inhibitor cocktail solution (Roche, Switzerland)) ...
-
bioRxiv - Immunology 2020Quote: ... The resulting membrane pellet was resuspended in ice cold 500 μl TNE buffer (10 mM Tris/Cl [pH 7.4], 150 mM NaCl, 5 mM EDTA, 1% Triton X-100 [Sigma], 10X protease inhibitors [Complete tablets, Roche, Indianapolis, IN]). Sucrose gradients for the preparation of lipid rafts were assembled previously described (18) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Endogenous peroxidase activity was blocked by incubation with 3% H2O2 for ten minutes followed by two washes with PBS containing 0.1% Tween-20 and blocking with PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk powder (Easis ...
-
bioRxiv - Genomics 2020Quote: ... cells were washed with buffer MB#1 (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche complete EDTA-free). Chromatin was fragmented with MNase for 10 min at 37°C and digestion was stopped with 5 mM EGTA at 65°C for 10 min ...
-
bioRxiv - Pathology 2020Quote: ... Coverslips were incubated overnight at 4 °C with a 1:100 dilution with one of the following primary antibodies: HA (Roche, #867423001), KDEL (Abcam ...
-
bioRxiv - Microbiology 2020Quote: ... and subjected to a protease protection assay by resuspension in 250 μl of freshly prepared 1 mg/mL Pronase (Roche, 10165921001)/0.01% Saponin/PBS at 12°C for 1h ...
-
bioRxiv - Molecular Biology 2020Quote: ... rocked at 4°C for 10 min and after centrifugation resuspended in 10 ml ice cold buffer LB2 (10 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x Roche cOmplete protease inhibitors), rocked at 4°C for 5 min and centrifuged again ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μl of each unpurified amplicon was then carried to a second PCR reaction using KAPA HiFi Library Amp (Roche Sequencing). NEBNext Multiplex Oligos (New England Biolabs ...
-
bioRxiv - Physiology 2021Quote: ... Collagen was then extracted from the tissue samples using a salt extraction buffer (1 M NaCl, 25 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease inhibitors (Roche, Basel, Switzerland). Samples were extracted overnight at 4°C with agitation ...
-
bioRxiv - Neuroscience 2020Quote: ... After centrifugation, cells were lysed (750 mM NaCl, 10 mM Tris, 1 mM EDTA pH 7.6, protease inhibitor (Roche, Mannheim, Germany)) ...
-
bioRxiv - Genomics 2021Quote: ... Four PCR reactions were then carried out with 1 μl of the purified DNA using the KAPA HotStart HiFi Ready Mix (KAPA Biosystems) for 10 cycles inserting a 15 nt homology sequence for the subsequent cloning step (IF_fwd/IF_rev) ...
-
bioRxiv - Immunology 2021Quote: ... and were enzymatically digested in a shaker incubator at 37°C for 40 minutes in RPMI medium containing 1 mg/ml Collagenase D (Roche-Diagnostics), 1 mg/ml hyaluronidase and 50 U/ml DNase I (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2019Quote: Cells were lysed in Tris-buffered saline (TBS) supplemented with 1% Triton X-100 and protease/phosphatase inhibitor cocktails (Roche Diagnostics), the insoluble material was cleared by centrifugation for 10 min at 20,000 × g ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... For large-scale purification cells were resuspended in lysis buffer (50 mM NaPO4 pH=7.0; 250 mM NaCl; 1 tablet complete protease inhibitor EDTA free (Roche, Basel, Switzerland) per 50 mL ...
-
bioRxiv - Physiology 2021Quote: ... containing cOmplete EDTA-free protease inhibitor cocktail at a concentration of 1 tablet/7 ml (Roche Applied Science, Indianapolis, IN, USA)) by repeated passage through a 20-gauge needle to obtain plasma membrane-enriched preparations ...
-
bioRxiv - Physiology 2020Quote: ... was homogenized by probe sonication in ice-cold membrane buffer (50 mM Tris-HCl pH 7.4, 1 mM EDTA, 250 mM sucrose, and complete protease inhibitors table (Roche Diagnostics, A32965)) ...
-
bioRxiv - Microbiology 2021Quote: ... the beads were collected on a magnetic stand for 2 min and washed four times with lysis buffer and four times with wash buffer (50mM Tris pH 7.6, 300mM NaCl, 1% Triton X-100, + complete protease inhibitor/ Roche Cat# 11836153001). The beads were resuspended in 1x Laemmli sample buffer ...
-
Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... for 3-4 hours at room temperature followed by overnight incubation at 4°C in anti-DIG-AP Fab fragments (1:5000) (Roche 1093274). Embryos were washed with PBST followed by an alkaline tris buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in 1% Triton X-100 for 30 min on ice followed by treatment with proteinase K (Roche 3115879001) at the indicated concentrations at 37°C for 5 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Fusion was carried out by resuspending the mixed cell pellet slowly in 1 mL of 50% PEG 1500 (Roche, Basel, Switzerland) at 37°C over the course of 1 minute and incubated for a further 1 minute at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: Cells were lysed in lysis buffer (50 mM Tris-HCl, pH 7.8, 150 mM NaCl, 1% Nonidet-P40 and protease inhibitor cocktail tablets (Roche, Basel, Switzerland)) ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 M KCl, 2 mM EDTA, 1 mM DTT, 0.01 U/ml benzonase and 1x EDTA-containing protease inhibitor cocktail [Roche Applied Sciences]). The lysate was cleared by centrifugation at 18,000 x g for 45 min at 4°C ...
-
bioRxiv - Microbiology 2020Quote: Frozen fecal samples were diluted in lysis buffer [100mg feces per 1 ml of PBS containing protease inhibitors (Roche, Indianapolis, IN)] ...