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Citations for Roche :
551 - 600 of 823 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The separated proteins were then electrotransferred onto a polyvinylidene fluoride (PVDF) membrane (Roche, Basel, Switzerland). Immunoblotting was conducted using a commercial HRP-conjugated Mouse anti GST-Tag antibody (Abclonal ...
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bioRxiv - Molecular Biology 2024Quote: Protein extraction was performed using RIPA buffer (Beyotime) supplemented with protease and phosphatase inhibitors (Roche). The protein concentration was determined using the BCA Protein Assay Kit (Epizyme Biomedical) ...
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bioRxiv - Immunology 2024Quote: ... Protein was then harvested after lysis in buffer containing a protease inhibitor (Sigma, 1183617001 Roche). Cell lysates were immunoblotted for β-actin and SCNN1γ ...
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bioRxiv - Plant Biology 2024Quote: ... The following antibodies were used for protein biochemistry experiments: αHA-Hrp (12013819001, Roche, 1/5000), αFlag-Hrp (A8592 ...
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bioRxiv - Molecular Biology 2024Quote: ... then mixed with 10 µL of protein G-agarose beads (PROTGA-RO, Roche, Basel, Switzerland) and incubated for 15 minutes on ice to capture proteins that non-specifically bind to the beads ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then treated with biotinylated Osteocalcin (Biotin Protein Labeling Kit, Cat# 11418165001, Roche, USA) with different concentration for 30 min at 37°C.
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bioRxiv - Cancer Biology 2024Quote: ... Protein - expression was detected with an OptiView DAB IHC Detection Kit (Roche Diagnostics, 760-700), optimized for automated immunohistochemistry (Benchmark GX stainer ...
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bioRxiv - Developmental Biology 2024Quote: Proteins were collected in ice-cold IPLS lysis buffer including protease inhibitor cocktail (#11873580001, Roche) 48 hours after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded human intestinal tissues was carried out on a Discovery Ultra automated slide stainer using a rabbit anti-human monoclonal antibody for CEA (Clone T84.66, Roche Glycart AG, Switzerland) at 2.23 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Microbiology 2020Quote: ... and 3-4 mice/group subcutaneously received 25ng/g of pegylated-human interferon α (Peg-hIFNα-2a) (Hoffmann La Roche, Basel, Switzerland). To activate IFN-1 signaling ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were blocked in 10% BSA and 0.05% Tween 20 in PBS for 30 minutes before application of one of the following antibodies: mouse anti-human p16INK4a (CINTEC-Roche, cat# 705-4793), rabbit anti-human alpha-smooth muscle actin or aSMA (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293 cells were transiently transfected with β-arrestin 2-Renilla luciferase 8 (Rluc8) and human C5aR2-Venus constructs using XTG9 (Roche, Sydney, Australia) for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of beta-globin was performed by a commercially available human genomic DNA kit (The LightCycler Control Kit DNA, Roche Diagnostics, Basel, Switzerland)69.
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bioRxiv - Cancer Biology 2022Quote: ... frozen human samples were disrupted in 600 µl of lysis buffer containing green ceramic beads using the MagNA Lyser Instrument (Roche Life Science, Germany). The cDNA synthesis was performed in a 20-µl reaction using random hexamers ...
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bioRxiv - Immunology 2024Quote: ... THP-1-monocyte-derived IFN-γ-primed macrophages were induced by co-stimulation with PMA and 100 U/ml human IFN-γ (Roche, 11040596001) for 24 h ...
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bioRxiv - Cell Biology 2020Quote: Protein was harvested in urea ammonium bicarbonate lysis buffer with complete EDTA free protease inhibitor (Roche) and phosphatase inhibitor (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with 60 µg of Protein A- or G-agarose bead slurry (Roche, Germany) for 4 hours at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Protein translation was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, 11411446001, Roche Diagnostics) and cultures were incubated overnight at 18 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein detection was carried out using peroxidase-coupled anti-HA-Peroxidase antibodies (Roche, dilution 1/4000), peroxidase-coupled anti-GFP antibodies (Milteneyi ...
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bioRxiv - Physiology 2021Quote: ... triglycerides and total protein were quantified using the Cobas c111 analyzer (Roche diagnostics GmbH, Mannheim Germany) as previously described by Segev-Hadar et al.38 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... cell culture supernatants were harvested and proteins were purified from the supernatants using tandem Ni2+ (Roche) and Strep-Tactin (IBA ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Biochemistry 2020Quote: ... coli cell pellets were resuspended in protein buffer containing cOmplete protease inhibitor cocktail (Roche, Basel, Switzerland), lysed by sonication and centrifuged for 45 min at 38,000 g to remove cell debris ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were subsequently detected with HRP-conjugated anti-HA antibody (rat monoclonal, Roche, catalog number 12013819001) or mouse monoclonal anti-GFP antibody (Novus Biologicals ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The protein extraction was performed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo ...
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bioRxiv - Microbiology 2020Quote: ... the supernatants were incubated in presence of 20 µl of protein G agarose beads (Roche, 11243233001) for 3 h at 4°C on a rotor to reduce unspecific binding ...
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bioRxiv - Biophysics 2020Quote: ... The expressed protein is first purified by affinity chromatography using Ni-NTA coated agarose beads (Roche). His6-tagged proteins are eluted using 250 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche) at 4°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and homogenized in a T-PER tissue protein extraction reagent supplemented with protease (Roche Applied Science) and phosphatase inhibitors (Millipore) ...
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bioRxiv - Neuroscience 2024Quote: ... and 250 mg or 500mg protein was digested with 20 µg/ml proteinase K (PK) (Roche) for PrPSc blots for 1 hour at 37C ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour (4 °C ...
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bioRxiv - Physiology 2024Quote: Protein cardiac extracts were obtained by tissue homogenization with ceramic beads (MagNa Lyser Green Beads, Roche) in lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were prepared from zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Roche), using a manual dounce homogenizer ...
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bioRxiv - Cell Biology 2023Quote: ... HBE were processed for protein lysates and stored at -20°C (Complete Lysis-M Roche diagnostics). NET dosage was an average of extracellular dsDNA concentration (QuantiFluor ONE dsDNA System ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... Cell lysates (1 mg) were pre-cleared with Protein-G–agarose beads (Roche, catalog number 11719416001) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The clarified lysate was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3 h at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... protein eluates from every purification step were immediately supplemented with protease inhibitors (EDTA-free cOmplete, Roche) (together with DTT at 10 mM for cysteine-containing protein constructs) ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were extracted with ice-cold RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Thirty μg of protein lysates were separated using SDS–polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Immobilion-P ...
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bioRxiv - Cancer Biology 2023Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche) for 2h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins with HA-tag(s) were detected using rat mAb-anti-HA 3F10 (Roche, 1:1000) as primary antibody and IRDye® 800CW Goat anti-Rat IgG (Licor ...
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bioRxiv - Cell Biology 2024Quote: Protein was extracted from RIPA buffer lysed cells supplemented with protease inhibitor cocktail (Roche, Basel, Switzerland). After quantification by Pierce BCA protein assay kit (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The recognized protein bands were visualized by 3,3 N-Diaminobenzidine tertrahydrochloride (DAB) solution staining (Roche, Germany).
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bioRxiv - Systems Biology 2024Quote: ... Protein concentrations were normalized to 1 mg/mL in PBS with Complete Ultra protease inhibitor (Roche). Intestinal and/or serum levels of LCN2 and IGF-1 were quantified using Mouse Lipocalin-2/NGAL DuoSet ELISA and Mouse/Rat IGF-I/IGF-1 DuoSet ELISA (R&D Systems) ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001). Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted proteins were subjected to 10% SDS-PAGE gels for immunoblot analyses using anti-GFP (Roche) and anti-Myc (Roche ...
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bioRxiv - Microbiology 2021Quote: ... transferred to PVDF membranes and protein expression was detected using anti HA (3F10, Roche 11867423001, 1:5000) followed by horseradish peroxidase (HRP)-conjugated anti-rat antibodies (Sigma ...