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Citations for Roche :
451 - 500 of 823 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Each resulting denatured protein sample was chemically reduced with 100 mM dithiothreitol (Roche) at 56°C for 30 min and alkylated using 100 mM iodoacetamide for 1 h ...
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bioRxiv - Immunology 2023Quote: ... After adding 50[μl of 50% of Protein-G bead (Roche Applied Science) to lysates and incubation with rotation at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes (175 mg protein) were treated with one cOmplete protease inhibitor tablet (Roche) then solubilised on ice at 4.5 mg mL−1 by the stepwise addition of lauryl maltose neopentyl glycol (LMNG ...
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bioRxiv - Biochemistry 2022Quote: ... 10% (v/v) glycerol) in the presence of cOmplete protein inhibitor cocktail (Roche). Both were then lysed in a cell disruptor (Constant Systems Ltd. ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by standard SDS-PAGE and transferred to PVDF membranes (Roche) using a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... Digestion of proteins was carried out by addition of trypsin (proteomics grade, Roche) at a 1/50 enzyme/protein ratio (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... Urine was analyzed for Protein and Albumin (Cobas c 311 Analyzer; Roche Diagnostics).
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bioRxiv - Molecular Biology 2023Quote: Predigested proteins and peptides were reduced with 10 mM dithiothreitol (DTT) (Roche, 10197777001) for 30 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % Brij97 (v/v) and phosphatase and proteins inhibitors (Roche, # 4906837001 and # 4693132001) for 30 min under end over end rotation at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: Total protein extracts were prepared in RIPA buffer with protease inhibitor cocktail (Roche). Samples were sonicated for 3 minutes alterning 30seconds ON/ 30seconds OFF ...
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bioRxiv - Cell Biology 2022Quote: Conjugation of protein or nanobody was performed with an 8-fold (Laminin; Roche) or 4-fold (R2-myc-his ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were used for immunoblot with anti-GFP (Roche #11814460001, 1:1,000), anti-RFP (Chromotek #6g6 ...
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bioRxiv - Neuroscience 2024Quote: ... and proteins were detected with a mouse antibody to GFP (1:2000, Roche), mouse antibody to FLAG (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies used for detection of proteins were rat anti-HA (3F10, Roche) (1:1000) ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC) and liver (human hepatocellular carcinoma, HepG2) cells were assessed using a standard WST-1 (Roche) cell viability assay ...
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bioRxiv - Microbiology 2020Quote: Damage to the human colon epithelial cell line HT-29 was assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of the LDH enzyme in the growth medium ...
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bioRxiv - Cell Biology 2020Quote: ... MEFs were seeded subconfluently o/n (unless indicated otherwise) onto coverslips acid-washed and coated with human fibronectin (25 μg/ml, #11051407001, Roche), as described (Dimchev and Rottner ...
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bioRxiv - Cancer Biology 2022Quote: ... All embryonic histology slides and human tissue microarray (BioMax, U.S. #BC001134b) slides were scanned using a Ventana DP200 slide scanning system (Roche Diagnostics) at 20x magnification ...
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bioRxiv - Genetics 2020Quote: A comprehensive list of coordinates of all the exonic and conserved regulatory elements from human X chromosome was used to design a customized capture library from Roche, NimbleGen (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2021Quote: Human pluripotent stem cells (hPSCs) were maintained in E8 medium and passaged every 4 days onto matrigel-coated plates (Roche). The following hPSC lines were used in the study ...
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bioRxiv - Developmental Biology 2021Quote: H9 human pluripotent stem cells were maintained in E8 media and passaged every four days onto matrigel-coated plates (Roche). ESCs ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC), and liver (human hepatocellular carcinoma, HepG2) [45–50] cells were assessed using a standard WST-1 (Roche) cell viability assay ...
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bioRxiv - Immunology 2023Quote: K2 cells or human neutrophils were lysed with 150-200 µl of lysis buffer (supp. Table 2) supplemented with 1X complete inhibitor (Roche) and incubated for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2024Quote: ... To detect for DNA containing complementary sequences on membrane-bound DNA a α-32P-dCTP-labelled probe spanning the region of 37-611 nts on human mtDNA was synthesized using High Prime DNA Labeling Kit (Roche). After pre-hybridizing the membrane with Church’s buffer (250 mM NaPi pH 7.2 ...
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bioRxiv - Neuroscience 2023Quote: Frozen brain tissues from human and animals were used to prepare 10% (w/v) homogenates in RIPA buffer containing PI and PhosStop (Roche). Briefly ...
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bioRxiv - Biochemistry 2023Quote: Cytotoxicity assays were performed as described29 (with minor changes. Proliferation of human cells was assessed using an MTT colorimetric assay (Cell Proliferation Kit I, Roche). HeLa (epithelial cells ...
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bioRxiv - Biochemistry 2024Quote: ... were transfected with human His-HA-RORβ (or pCMV6 base vector for control samples) using Xtreme Gene HP DNA reagent (Roche) at 70 to 80% confluency at a ratio of 3:1 reagent to DNA ...
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bioRxiv - Developmental Biology 2024Quote: Human pluripotent stem cells (hPSCs) were maintained in E8 medium and passaged every 4 days onto matrigel-coated plates (Roche). The following hPSC lines were used in the study ...
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bioRxiv - Cancer Biology 2024Quote: ... The CD8+ and FoxP3+ cells present in these human tissues were identified by immunohistochemistry double stain using a DISCOVERY ULTRA automated stainer (Roche) followed by analysis using CaloPix ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted by incubation with RIPA buffer and protease inhibitor cocktail (Roche, 4693159001) on ice for 1 hour with occasional vortexing ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) containing cOmplete EDTA-free protein inhibitor cocktail (Roche, 1 tablet/10 ml), lysozyme (1.0 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested using Endoproteinase Lys-C at 1:100 w/w (Roche, 11058533103) at 37 °C overnight ...
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bioRxiv - Physiology 2020Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was removed by the addition of 100 µl homogeneous Protein G-Agarose (Roche) and a further incubation for 3 h ...
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bioRxiv - Developmental Biology 2022Quote: ... The lysate was precleared by incubation with 50 μL protein-A agarose beads (Roche) for 1 h and then incubated with anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were generated using RIPA lysis buffer supplemented with protein inhibitor (Roche, #4693159001) and protein concentration was determined using Pierce™ BCA protein assay ...
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bioRxiv - Cancer Biology 2020Quote: ... Separated proteins were then transferred to a methanol-activated polyvinylidene difluoride membrane (PVDF, Roche) using wet transfer Mini-PROTEAN II electrophoresis system (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were then treated with 8 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 3 hours followed by 19 μg/sample of trypsin (Roche Cat ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were then extracted in an IP buffer with a phosphatase inhibitor (Roche, PhosSTOP), centrifuged at 1700g for 5 min and filtered through miracloth ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and chromatin quality analysed using EtBr agarose gel electrophoresis (1.3 % Biozym ME Agarose ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and analysed using ethidium bromide (EtBr ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol (TS130G) in the presence of cOmplete protein inhibitor cocktail (Roche). The resulting samples were clarified by centrifugation in an SS34 rotor (27,000 xg ...
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bioRxiv - Cell Biology 2020Quote: ... followed by addition of 5µl (packed volume) of protein G-coupled Agarose beads (ROCHE) and continued overnight incubation at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Protein was detected with 1:2,000 horseradish peroxidase (HRP)-conjugated anti-HA antibody (Roche).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested by adding 1 mL of Proteinase K (20 mg/mL) (Roche) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... blocked in 5% nonfat dry milk and proteins detected using monoclonal anti-GFP (Roche), monoclonal anti-PGK (Invitrogen) ...