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Citations for Roche :
551 - 600 of 8815 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... for 2 hours at room temperature and then incubated in anti-DIG-AP (Roche, 1:2000) overnight at 4°C and washed and developed with Fast Blue as described in (King 2013) ...
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bioRxiv - Biochemistry 2021Quote: ... 2×106 cells HEK293T cells were transfected with 2 μg plasmid DNA using X-tremeGENE HP DNA Transfection Reagent (Roche) in 10-cm dishes ...
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bioRxiv - Cell Biology 2024Quote: ... The parasites were pelleted by centrifugation at 25,000 x g and resuspended in 5 × the pellet volume with hypotonic lysis buffer (1 mM HEPES-NaOH pH 7.4, 2 mM EGTA, 2 mM DTT with protease inhibitor cocktail; cOmplete™, EDTA-free, Roche). The parasite suspension was incubated for 10 min on ice and then lysed by approximately 30 passages through a 1mL syringe fitted with a 27G needle.
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bioRxiv - Immunology 2023Quote: ... dissociated from the coverslips in 8 M urea lysis buffer (100 mM NaCl, 25 mM Tris, 2% SDS, 0.1% tween 20, 2 mM EDTA, 0.2 mM PMSF, and 1x Roche cOmpleteProtease inhibitor). Lysates were treated with benzonase (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Whole lymph nodes or chopped spleens were then transferred into 15 ml conical tubes containing 2 ml digestion buffer (RPMI containing 2% FBS, 0.1mg/ml DNAse I (Roche, #10104159001), 0.2 m/ml collagenase P (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... 1% PMSF (Roche Life Sciences, 329-98-6), 25 U benzonase nuclease (EMD ...
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bioRxiv - Genomics 2021Quote: ... on a gentleMACS Octo Dissociator (Miltenyi) using the “Protein_01_01” protocol in MACS buffer (5 mM CaCl2, 2 mM EDTA, 1X protease inhibitor (Roche, 05-892-970-001), 300 mM MgAc ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellets were lysed in 200μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 5 mM MgAc, 2% digitonin, and 1X Roche protease inhibitor cocktail) by incubating on ice for 30 min and then diluted to 1ml with the lysis buffer containing 0.1% digitonin ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer F (20 mM Tris pH 7.5, 100 mM NaCl, 5 mM MgCl2, 2 mM EGTA and Roche cOmplete protease inhibitor) and lysed by fluidizer ...
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bioRxiv - Cell Biology 2022Quote: ... and then homogenized on a gentleMACS Octo Dissociator (Miltenyi) using the “Protein_01_01” protocol with MACS buffer (5 mM CaCl2, 2 mM EDTA, 1X protease inhibitor (Roche, 05-892-970-001), 3 mM MgAc ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were resuspended in Lysis Buffer (25 mM HEPES pH 8.0, 250 mM NaCl, 10 mM imidazole pH 8.0, 5 mM 2-mercaptoethanol, 10% glycerol and supplemented with Roche cOmplete protease inhibitor) and sonicated ...
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bioRxiv - Physiology 2023Quote: ... autofluorescence was quenched by treating paraffin-embedded sections with PBS/BSA (5%) for 2 h before performing TUNEL staining (Roche, Basel, Switzerland) according to the manufacturer’s protocol and using Proteinase K treatment ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µl of the samples’ cDNA was mixed with 5 µl of FastStart SYBR Green Master (ROX; Hoffmann-La Roche, Basel, Switzerland), 0.25 µl of each primer (final concentration 500 nM) ...
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bioRxiv - Biochemistry 2022Quote: Proteins were extracted from adherent cells by scraping into extraction buffer (1× LysisM, 1× protease inhibitor cocktail, 2 x phosphatase inhibitor cocktail (all Roche), 2 mM sodium orthovanadate (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and low-volume supernatants (90 μL media per well of a 48-well plate) were mixed 1:1 with 2× SDS/PAGE sample buffer containing Complete Mini EDTA-free Protease Inhibitor Mixture (Roche). In experiments where primary hMDMs were plated in a 24-well plate and infected with T4SS-Lp ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were washed three times with TBST and then incubated with appropriate secondary antibodies (2 µg/mL in TBST / 1% bovine serum albumin (BSA)) and 1 µg/mL DAPI (Roche) for one hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked for 2 hours at room temperature in 1% blocking solution (1% Western Blocking Reagent (WESTBL-RO, Roche) in TBS ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl pH 7.5, 300 mM NaCl, 1 mM DTT, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]), and lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM EDTA with 1x protease inhibitors (Roche). Protein concentrations were quantified with a BCA Kit (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... For cDNA synthesis 2 μg of DNaseI (Roche) treated total RNA was reverse transcribed using M-MuLV reverse transcriptase (Minotech) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl 5x KAPA HiFi buffer (Kapa Biosystems), 0.3 µl 10 mM dNTPs (Kapa Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... and/or DAPI (Roche: 10236276001, 2 μg/mL) before mounting to visualize barrel map ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 100 U/mL Il-2 (Roche). K562 cells were obtained from ATCC (CCL-243 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing 2 mg/ml Collagenase A (#10103586001, Roche), 2.4 U/ml Dispase II (#04942078001 ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μg/mL DNAse (11284932001, Roche Diagnostics), and incubated for at least 10 min on ice before sonication (10 cycles of 15 s with 30 s cooling at 8 microns amplitude ...
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bioRxiv - Molecular Biology 2020Quote: ... 2% BSA fraction V (Roche Diagnostics, Mannheim, Germany), 10 mM nicotinamide (Merck Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mg/ml protease inhibitor cocktail (cOmplete, Roche)] on ice for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 2 tablets of the protease inhibitor cocktail (Roche) per 100 mL] ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 tablet of complete protease inhibitor cocktail (Roche) 2mM DTT ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... anti-PD-L1 (atezolizumab, Roche, 2 mg/kg), anti-TGF-β (mouse IgG1 clone 1D11 from BioXCell ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... anti-PD-L1 (atezolizumab, Roche, 2 mg/kg), or a combination of anti-PD-L1 (2 mg/kg ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 µg/mL Liberase Blendzyme 2 (Roche, 05401020001) and 5 ml of Penicillin/Streptomycin (Gibco Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and 100 U/ml of interleukin-2 (Roche) (complete IMDM) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM EDTA with 1x protease inhibitors (Roche). 50 ug lysate was used for label free quantification at the Rockefeller University Proteomics Core Facility ...
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bioRxiv - Bioengineering 2021Quote: ... and 2 mg/ml DNase I (Roche, 11284932001) to obtain a single cell suspension.
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bioRxiv - Cell Biology 2020Quote: ... and 2 μg/ml collagenase A (Roche 10103586001). Tissue preparations were incubated at 37 °C in a water bath (in gently shaking and not in immersion ...
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bioRxiv - Immunology 2021Quote: ... then with Collagenase/Dispase (2 mg/mL) (Roche) and DNase I (2 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl 5x KAPA HiFi buffer (Kapa Biosystems), 0.3 µl 10 mM dNTPs (Kapa Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... and IL-2 (50/250 IU/ml) (Roche). The glucose medium consisted of glucose-free RPMI 1640 (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 μl alkaline phosphatase (Roche Basel, Switzerland) were added and incubated overnight in the dark at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 2 mg/ml Lysozyme and protease inhibitors (Roche) per 1 L of pelleted culture ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... and 2 cOmpleteTM mini protease inhibitor cocktail (Roche) tablets were added to the cell suspension followed by cell lysis using a French Press (Stansted) ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... Terminal transferase (2 µl/ml; Roche, Basel, Switzerland) and biotinylated dUTP (1 µl/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2 mg/ml collagenase/dispase (Roche, 10269638) at 37°C for 40 min with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mg/ml collagenase A (#1013586001, Roche, Switzerland), 0.2 mM CaCl2 (#C5670 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM EDTA complemented with phosphatase ihibitor (Roche Diagnostics Scandinavia AB ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... 2 µl/ml Benzonase) containing protease inhibitors (Roche) and sonicated for 2 min (5’’on/5’’off ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μl of micrococcal nuclease (Nuclease S7, Roche) was added and the lysates were incubated at 25 °C for 18 min using a thermal cycler (Techne-Prime ...