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Citations for Roche :
5651 - 5700 of 8095 citations for 7 Bromoacetyl 6 ethyl 1 2 3 4 tetrahydro 1 1 4 4 tetramethylnaphthalene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Cells were resuspended in cold homogenization buffer (250 mM sucrose, 250 mM HEPES/KOH pH 7.4, 1 mM EDTA) supplemented with cOmplete protease inhibitors (Roche) and lysed by Dounce homogenization on ice ...
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bioRxiv - Neuroscience 2023Quote: ... 7.5×105 of S2 cells were lysed in Lysis Buffer (50 mM Tris at pH 6.8, 150 mM NaCl, 1% NP40, protease inhibitor cocktail [Roche]), immunoprecipitated with 1 μg of GFP antibody (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... for 10 min and resuspended in lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris pH 8.1) supplemented with protease inhibitors (Roche) and incubated on ice for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently washed with PBS three times and nuclei were counterstained with DAPI (2.5 μg ml−1, Roche) before mounting with Prolong Gold (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were next rinsed 3x 15 minutes in room temperature TBST (Tris-buffered saline [pH 8.5] with 1% Tween 20) and blocked for two hours in 20% DIG buffer (Roche) in TBST.
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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Brief Change in Dopamine Activity during Consolidation Impairs Long-Term Memory via Sleep DisruptionbioRxiv - Neuroscience 2023Quote: ... primary antibodies used to detect GRASP signals was mouse monoclonal anti-GFP (1:200, Cat# 11814460001, Roche Applied Science). Samples were then washed in PBS-T at 4 ℃ for 10 min for 3 times ...
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bioRxiv - Biochemistry 2023Quote: ... under gravity flow, washed with PER2 buffer (1 x TBS pH 7.5, 1mM EDTA, 5% glycerol, EDTA-free protease inhibitors (Roche)) and eluted with 3x flag peptide in PER2 buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with horseradish peroxidase (HRP)-conjugated anti-DIG (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010 ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5) (10 mL/g pellet weight) supplemented with DNAse and 1 cOmplete EDTA-free Protease Inhibitor Cocktail tablet (Roche) per 2 L of culture ...
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bioRxiv - Microbiology 2023Quote: ... blocked in 5 % milk and probed with α-GFP (1:1,000 in 5 % milk; Roche Diagnostics GmbH, Mannheim, Germany), for GFP-based sensor probing ...
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bioRxiv - Genomics 2023Quote: ... The cell pellet was resuspended with ice-cold Galbraith’s buffer amended with bovine serum albumin (BSA, 0.05% w/v) and cOmplete protease inhibitor (1×, Roche 11697498001), lysed by repeated pipetting ...
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bioRxiv - Immunology 2023Quote: ... euthanized mice were perfused with PBS and the left lung lobes digested for 1 h with collagenase D (1.5 mg/ml, Roche) and DNAse A (40 U/ml ...
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bioRxiv - Immunology 2023Quote: ... Popliteal lymph nodes were harvested from immunized mice 1 day later and digested with collagenase D (2.5 mg/mL, Roche) in RPMI-1640 medium supplemented with 10% FBS for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The nuclear pellets were resuspended in nuclear lysis buffer (50mM Tris-HCl pH 8.0, 10mM EDTA, 1% SDS, 1mM PMSF, cOmplete Protease Inhibitor Cocktail (Roche)) and vortexed at 4°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in buffer M (50 mM NaH2PO4, 50 mM Na2HPO4, pH 6.8) supplemented with 1 mg/ml lysozyme and protease inhibitor (Roche) and opened by sonication ...
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bioRxiv - Cell Biology 2023Quote: ... Mixed-stage transgenic worms expressing ectopic GFP::EGO-1 were collected and resuspended in equal volumes of 2x lysis buffer (50 mM Tris-HCl [pH 8.0], 300 mM NaCl, 10% glycerol, 1% Triton X-100, Roche ®cOmplete EDTA-Free Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE slides were steamed for 48 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue slides were steamed for 32 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in ice cold lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0) supplemented with protease inhibitors (Roche) and incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 million cells were suspended in 500 μl SDS lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, protease inhibitor (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: Single cell suspensions were obtained from dissected muscles mechanically disrupted and enzymatically digested at 37 °C for 1h using DMEM 1% P/S medium containing 0.2% collagenase B (Roche) and Calcium dichloride (CaCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... target proteins were detected by using primary antibodies of mouse monoclonal anti-GFP antibody (1: 2000; Roche, Cat. 11814460001), mouse anti-mCherry (1:5000 ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in 750 μl Tris lysis buffer (50 mM Tris, 0.2% NP-40, 150 mM NaCl, 5 mM EDTA, 5.7 mM PMSF, and 1 x Roche cOmplete). For whole organ samples (either whole detunicated testis or thymus) ...
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bioRxiv - Biochemistry 2023Quote: ... washed with 1 x PBS and resuspended in 100 μl of SoTE buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.15 % Triton X-100, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: ... washed once in PBS and lysed for 15–20 min on ice with lysis buffer A (50 mM Tris, pH 7.8, 1% NP-40, 150 mM NaCl) containing protease inhibitors (Complete, Roche), 2-mercaptoethanol ...
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bioRxiv - Genetics 2023Quote: VSMCs and myofibroblasts were scraped in phosphate-buffered saline (PBS) supplemented with protease inhibitor cocktail (1:100, Roche, 11836145001) and phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Immunology 2023Quote: Tumors were mechanically homogenized using a Dounce Tissue Homogenizer and enzymatically digested using 0.05mg ml-1 Liberase TL (Roche), 0.2mg ml-1 Dnase I (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted proteins were separated on 8% SDS-PAGE gel (37.5:1 acrylamide:bis-acrylamide) and probed with anti-HA (Roche, 3F10), anti-GFP (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... The bacterial pellet was then resuspended in ice-cold lysis buffer composed of 1x PBS (pH 7.4) and 1 tablet of protease inhibitor (Roche) per 10 ml ...
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bioRxiv - Biochemistry 2024Quote: Cells were lysed in native lysis buffer (10% glycerol, 25 mM NaCl, 20 mM HEPES pH 7.0, 1% DDM) supplemented with protease inhibitor tablet (Roche), and solubilized by rotating 30 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with primary antibody for 2.5 h (GFP, Abcam, Cambridge, UK: rabbit, 1:10000; HA (Roche), rat ...
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bioRxiv - Physiology 2024Quote: ... cells were lysed in IP buffer (10 mM Tris pH 8, 0.4% NP40, 300 mM NaCl, 10% glycerol, 1 mM DTT, antiprotease (Roche), anti-phosphatase (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 1µg of DNase from bovine pancreas and 1 dissolved tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Pellets were then homogenized with a Biospec Tissue Tearor (Dremel) ...
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bioRxiv - Biochemistry 2024Quote: His-tagged 53BP1 TTD was purified by resuspending thawed cell pellets in 30ml of lysis buffer (50mM sodium phosphate pH 7.2, 50mM NaCl, 30mM imidazole, 1 x EDTA free protease inhibitor cocktail (Roche) per litre of culture) ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then harvested by centrifugation and resuspended in buffer A (20 mM Tris-HCl, 1 mM EDTA, pH 8.0) including a protease inhibitor tablet (EDTA-free, Roche). Cells were lysed by sonication on ice before cell debris was removed by centrifuged (18,000 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were lysed in SDS based lysis buffer (50 mM Tris Ph7.4, 150 mM NaCl, 1% SDS, 1mM EDTA and 50mM NaF) containing protease inhibitors (Roche) and sonicated ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using NP-40 lysis buffer (100 mM Tris pH 8.0, 1% NP-40, 150mM NaCl) containing protease inhibitor cocktail (Roche) for 20 min with shaking on ice ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 1µg of DNAse from bovine pancreas and 1 dissolved tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Lysates were incubated with Ni-NTA agarose (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellets were resuspended in 150 μl chilled lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.1, 1x protease inhibitor cocktail, 1x PhosSTOP phosphatase inhibitors (Roche), 5 mM sodium butyrate (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and supplemented with 850 μl 1% Triton X IP dilution buffer (1% Triton X, 20mM Tris-HCl pH8.1, 150mM NaCl, 2mM EDTA, 1x protease inhibitor cocktail (Roche), 1x PhosSTOP phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... each sample was normalized to 3.5 ng in 5 µL water before generating libraries using a 1:10 Kapa HyperPlus (Roche) miniaturized protocol with barcoded indices as previously described82 ...
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bioRxiv - Microbiology 2024Quote: ... and 10% glycerol) supplemented with the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche), frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 1/10 volume of lysis buffer (0.2 M Tris-HCl, pH 8, 10 mg/mL lysozyme, and 1× EDTA-free protease inhibitors; [Roche]), incubated for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended with 1 ml of single nuclei wash buffer containing 0.2 U/µl RNase inhibitor (3335402001, Roche) and transferred to a microcentrifuge tube ...
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bioRxiv - Microbiology 2024Quote: ... 500 μg cell lysate was diluted to 500 μL in lysis buffer supplemented with 1 mM DTT and 25 μg poly(dI:dC) (Roche) and precleared by incubating with 50 μL streptavidin beads for 1 hour at 4°C on rotating wheel ...
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bioRxiv - Molecular Biology 2024Quote: Cells were lysed in DDM lysis buffer (50 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM EDTA; 50 mM NaF; 1mM sodium vanadate; 1x Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... Dicer was detected using anti-HA 3F10 monoclonal primary antibody (High Affinity rat IgG1, Roche #11867431001; dilution 1:500), anti-HA rabbit primary antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extraction buffer (150 mM NaCl, 1% Triton x-100, 50 mM TrisHCl pH 7.4 with protease inhibitor (11836170001 Roche)) ...
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bioRxiv - Genomics 2024Quote: ... Isolated nuclei were then lysed with lysis buffer (50 mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor complete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...