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Citations for Roche :
5501 - 5550 of 6054 citations for 7 METHYL 1 INDANONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Xenograft OS tumors were harvested and microdissected 12 d after 143B OS cell inoculation and digested with collagenase type I and II (1 mg/ml; Roche) digestion for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were washed 3x 15 minutes in room temperature TBST (Tris-buffered saline with 1% Tween 20) and blocked at room temperature for one hour in 10% DIG buffer (Roche) in TBST.
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bioRxiv - Immunology 2024Quote: Cell lysates were prepared by lysing cells for 1 h at 4 °C in RIPA buffer supplemented with cOmplete protease inhibitor cocktail (Roche). The cell lysate concentrations were quantified using a BCA protein assay kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Total proteins from T-ALL#1 cells were extracted with a RIPA buffer containing a cocktail of 1Xprotease inhibitors (11836145001, Roche) and 1X phosphatase inhibitors (P2850 ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies used were prepared in 2% BSA (diluted in PBS) and used at the following concentrations: rat monoclonal anti-HA (1:1000, 3F10 Roche), mouse monoclonal anti-myc (1:100 ...
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bioRxiv - Immunology 2024Quote: ... Quantitative RT-PCR analysis was performed with selective primers (Supplementary Table 1) using the Light Cycler 480 SYBR Green I Master X2 Kit (Roche), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Human and murine cells were incubated in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 % NP-40, 5 % glycerol) containing 1X protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Immunology 2024Quote: ... Probed membranes were washed and incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit Abs for 1 hour and developed with Lumi-LightPLUS Western Blotting Substrate (Roche). Quantification was performed on Lumi-Light autoradiography films using ImageJ software.
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bioRxiv - Immunology 2024Quote: ... whole pituitaries or separate posterior and anterior pituitaries were mechanically lysed by suspending and digested with 50 µg/ml DNase 1 (Roche) and 1 mg/ml Collagenase D (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Lobes were separated into clusters of 1-5 oocytes and the follicular layer was removed i) enzymatically with Liberase (Roche) and ii ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with a 20-fold excess of unlabeled oligonucleotide with the same sequence as the labeled one (to prevent reannealing) and 1 µl of Proteinase K (18 mg/ml) (Roche). After deproteination for 30 min at 37 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and lysed in 50 μL of lysis buffer (50 mM Tris, pH 8, 150 mM NaCl, 1% Triton-X-100, 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]) at 4 °C for 15 min ...
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bioRxiv - Genomics 2024Quote: ... were digested for 1 hour at 37°C under 110 rpm shaking conditions in DMEM supplemented with 2 mg/mL collagenase D (Roche) and 0.4 mg/mL Dispase (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... 1 μg of total RNA was reverse transcribed using the Roche Transcriptor First Strand cDNA synthesis kit (Roche Life Sciences) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... and lung lobes were finely minced and incubated in 15 mL of complete media containing 1 mg/mL collagenase (Roche) and 17 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... The agarose was suspended in dilution buffer for IPs from cell lysates and in a 2 : 1 lysis buffer : dilution buffer mix for IPs from EC fractions and supplemented with cOmplete Protease Inhibitor Cocktail (Roche). Of the 130 µl EC fraction/cell lysate ...
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bioRxiv - Cell Biology 2024Quote: ... dissected TA muscles were first digested with 10ml muscle digestion buffer (DMEM containing 1% penicillin/streptomycin, 0.125mg/ml Dispase II (Roche, 04942078001), and 10mg/ml Collagenase D (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 5 mL of either test buffer supplemented with 1 mM phenylmethylsulfonyl fluoride and one protease inhibitor tablet (Roche) was then to resuspend the fine powder ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were lysed in NP40 cell lysis buffer (10mM Tris-Cl, pH 7.4, 1% NP40, 1X Roche Complete protease inhibitors) at 4C for 1 hour followed by centrifugation at 18,000 g for 10 mins and supernatant collected as sample for analysis ...
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bioRxiv - Genomics 2024Quote: ... Indexing PCR was performed to add Nextera index adapters (1 μM final, Integrated DNA Technology) using KAPA HiFi reagents (#KK2102, Roche). Libraries were pooled in equal volumes and a final 0.8x cleanup was performed with homebrew SeraMag beads before measuring the sample concentration and sizing ...
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bioRxiv - Immunology 2024Quote: ... LNs obtained from organ donors were submerged in 5 mL R10 media (RPMI + 10% FBS + 1% penicillin/streptomycin + 2mM L-glutamine) supplemented with 10U/mL DNase I (Roche), cleaned of visceral fat ...
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bioRxiv - Neuroscience 2024Quote: ... antibody in-situ stainings were done as described previously (Haussmann et al., 2008) for validation using rat anti-HA (MAb 3F10, 1:20; Roche), rabbit anti-GFP (Molecular Probes ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by discarding 100 ul from each well and adding 100 ul of solution containing DMEM with 10% FBS and WST-1 reagent (Roche Diagnostics) diluted 1:10 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 μL of each primer at 10 μM and 0.5 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc, A Roche Company) for a total volume of 25 μL ...
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bioRxiv - Cancer Biology 2021Quote: ... For CO-IP MS analysis JJN-3 KO and WT cells were incubated in hypoxia for 24 hours before lysis with 4x pellet volume RIPA buffer (1% CHAPS, 50 mM Tris, 150 mM NaCl, Complete protease inhibitor (Roche Diagnostics) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... pH 7.5) containing protease inhibitor cOmplete Tablets EASYpack or phosphatase inhibitor PhosSTOP EASYpack (each 1 tablet per 15 mL, Roche, Basel, Switzerland), and diluted in 6×SDS-PAGE sample loading buffer (Beyotime ...
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bioRxiv - Cell Biology 2020Quote: ... 1μg of this material was then ligated in a volume of 20 μl with 1 μl of T4 DNA ligase (Roche, 10799009001) at 16°C to generate a randomized library of large fragments ...
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bioRxiv - Immunology 2021Quote: Single-cell suspensions of tumors were generated by dicing tumors with a razor blade followed by enzymatic digestion for 30 minutes at 37C in medium containing 1 mg/mL Collagenase D (Roche Diagnostics) and 1μg/mL DNase I (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Pooled tissue from each brain region was suspended in 6 ml of 0.32 M sucrose homogenization buffer (4 mM HEPES, 0.1 mM CaCl2, 1 mM MgCl2, plus Roche protease inhibitor tablet) and homogenized with a Teflon homogenizer using 10 strokes at 900 rpm ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA for each sample using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche, 05081963001), with a mixture of oligo dT and random primers ...
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bioRxiv - Immunology 2021Quote: Total protein lysates from MDDC and cDC cultured for 1 h in the presence of media or individual or combined Poly I:C and 2′3′-di AM(PS) agonists were obtained using RIPA buffer containing 1% phosphatase and protease inhibitors (Roche Diagnostics). Subsequently ...
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bioRxiv - Genomics 2020Quote: ... This was followed with primary antibody incubation performed with 1:1000 goat anti-SOX17 (R&D) diluted with Antibody Dilution Buffer (Roche Ventana) for 60 minutes at 37°C ...
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bioRxiv - Pathology 2019Quote: ... The slides were incubated in MRPL12 primary antibody (HPA022853, MilliporeSigma) at 1:200 dilution in Antibody Dilution Buffer (ADB250, Roche, Switzerland) for 45 min at RT ...
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bioRxiv - Genetics 2020Quote: ... and the cDNA was diluted 1/10 for qPCRs using MESA BLUE MasterMix (Eurogenentec, 10-SY2X-03+NRWOUB) on a LightCycler® 480 Instrument II (Roche). A list of primers used can be found in Supplementary Table 7.
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bioRxiv - Biochemistry 2019Quote: ... This pellet was then resuspended in Buffer A (20 mM Tris-HCl pH8.0, 1 mM EDTA, 5% glycerol, 0.1 mM PMSF, 5 mM DTT, Roche protease inhibitor cocktail) up to a volume where the conductivity of the solution was 16 mSv ...
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bioRxiv - Cancer Biology 2019Quote: ... The nuclear pellet was brought up to 880 μL in Nuclear Lysis Buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.5% SDS, 1× Roche protease inhibitors, 11697498001), and sonicated with a Bioruptor 300 (Diagenode ...
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bioRxiv - Pathology 2019Quote: ... cDNA synthesis was performed with a 1 μg RNA sample using the First Strand cDNA Synthesis kit RT-PCR (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were resuspended in approximately five volumes of lysis buffer (50 mM NaH2PO4 pH 8, 1 M NaCl, 10 mM Imidazole, 2 mM PMSF and protease inhibitor cocktail (Roche #4693132001). Cells were lysed by sonication on ice for 12 x 30 seconds at 30 W with one minute on ice in between ...
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bioRxiv - Plant Biology 2019Quote: ... Blots were blocked for at least 1 hour in blocking solution at RT (5% milk in 1xTBS) before probing with primary antibody in blocking solution (α-HA-HRP, 1:2500 (Roche); α-PGB1 ...
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bioRxiv - Immunology 2019Quote: ... and each of the three nested PCR steps was carried out in a total volume of 10 µL using 1 µL cDNA/PCR template and KAPA HiFi HotStart ReadyMix (Kapa Biosystems). In the last PCR reaction ...
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bioRxiv - Cancer Biology 2019Quote: ... PD-1 C192S mutant was generated by site-directed mutagenesis PCR reaction using platinum PWO SuperYield DNA polymerase (Roche, Basel, Switzerland) according to the product manual ...
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bioRxiv - Microbiology 2019Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
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bioRxiv - Genomics 2019Quote: ... The pellet was resuspended in sonication buffer (1% SDS, 50mM Tris pH8, 10 mM EDTA, 1x protease inhibitor (cOmplete™, Mini, EDTA-free, Roche)) and sheared for 25 minutes (30sec ON ...
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bioRxiv - Developmental Biology 2019Quote: ... Hybridisations were performed overnight at 65°C then samples were incubated with an antibody against anti-digoxigenin conjugated with AP Fab fragments (1:2000; Roche, 11093274910). Staining was done using NBT/BCIP (Roche).
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bioRxiv - Genetics 2019Quote: ... which was diluted 1:10 to be used in RT-qPCR carried out using LightCycler 480 SYBR Green I Master (Roche, 4707516001) under the following conditions ...
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bioRxiv - Genetics 2019Quote: ... was used as a hypotonic solution and cells were dropped onto a slide with 1% PFA containing protease inhibitors (Roche 1836153). After 3h at 4°C slides were washed and blocked with 0.5x blocking buffer (1.5% BSA ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were re-probed to detect ZMPSTE24 and Rce1 using rat anti-HA (clone 3F10, Roche cat #11867423001; 1:10000 dilution), and polyclonal rabbit anti-Rce1 [52] (1:2000 dilution) ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche], 2 U/mL DNase I [Life Technologies] and Dispase II [Roche]). The digested brain parenchyma ...
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bioRxiv - Immunology 2021Quote: ... 1mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1x Roche Complete Protease Inhibitor Cocktail, and 1x Roche Phosphatase Inhibitor Cocktail). Protein concentrations were measured using a BCA kit (Pierce) ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 1 mM DTT and incubated at RT in 0.05 mg/mL sequencing grade trypsin (Roche Applied Science). Digestions were stopped with 2X Laemmli sample buffer and placed on ice until analysis ...