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Citations for Roche :
5351 - 5400 of 6054 citations for 7 METHYL 1 INDANONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Cells were harvested and stored at -80°C in ICD lysis buffer (300 mM NaCl, 50 mM Tris/HCl, 1 mM EDTA, 1x Protease Inhibitor (Roche), pH 7.4) ...
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bioRxiv - Pathology 2024Quote: ... cDNA product was 1 to 40 diluted and real-time PCR was performed using FastStart SYBR Green Master Mix (Roche) on QuantStudio 5 (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl of IPed proteins and 10 µl of WCE were submitted to SDS-PAGE and analyzed by immunoblotting using anti-HA antibody (1:250, ref.12013819001, Roche), anti-Flag antibody (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were permeabilised by 10 minutes incubation with 0.1% Triton X-100 on ice and incubated with blocking buffer (10% FBS in 1X PBS) for 90 minutes and then probed with the primary antibody (anti-HA rat 1:100 (Roche) for 2 hours on rotor and after three washing with 1x PBS cells were probed with anti-rat Alex Flour-488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: Frozen liver tissues that were crushed using a Multi-beads Shocker (Yasui Kikai, Japan) or cultured cells were dissolved in RIPA buffer containing 1× cOmplete proteinase Inhibitor Cocktail (Roche). Protein concentration in the lysates was determined using a BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... and collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor [#11873580001, Roche] in PBS). Chromatin was sonicated with a Covaris E220 sonicator (140PIP ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The final pellet was resuspended in 750 uL nuclear lysis buffer (10 mM EDTA, 1% SDS, 50 mM Tris-HCl at pH 8, 1x cOmplete protease inhibitor cocktails (Roche)) ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MAB-Buffer2 (20% sheep serum, MAB-Buffer1) for 1h and incubated with anti-DIG-AP antibody (1:4000, Roche) in MAB-Buffer2 overnight at 4 °C.
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bioRxiv - Immunology 2024Quote: ... Lungs were resected and minced with scissors before incubation in a collagenase buffer containing 1 mg/mL Collagenase-A (Roche), 2000U DNaseI (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... whole cell lysate samples were prepared by resuspension and trituration of cell pellets in a lysis buffer (20 µM Tris-HCl pH 7.4, 1 µg/mL DNaseI, 1X cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA and 1% Triton X-100) containing 2 mM Na3VO4 (Sodium Orthovanadate) and 1 mM pmsf (phenylmethylsulfonyl fluoride) and 1x protease inhibitor cocktail tablet (Roche). Whole cell lysates were centrifuged at 12,000 rpm at 4 0C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the supernatant replaced by 1ml PBS-Glucose supplemented with 50 µl Collagenase/Dispase (Worthington CLS-1 345U/mg; Dispase II Roche 1.2U/mg ...
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bioRxiv - Synthetic Biology 2023Quote: ... cell pellets were resuspended in 10 ml cold TES buffer (50 mM Tris pH 8.0, 20% Sucrose, 1 mM EDTA) supplemented with EDTA-free complete protease inhibitor (Roche, Switzerland) and incubated at 4 °C for 45 mins ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked with freshly prepared blocking solution (100 mM maleic acid, 150 mM NaCl, pH 7.5, 1% (wt/vol) blocking reagent (Roche, 11096176001)) for 30 min ...
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bioRxiv - Genomics 2023Quote: ... The membrane was then extensively washed with low and high stringency buffers and subsequently blocked with buffer B2 (1% Blocking powder [Roche] in buffer B1 [100 mM Maleic acid ...
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bioRxiv - Immunology 2023Quote: ... lung and liver tissues were mechanically disrupted into small pieces and enzymatically digested in 2ml of digestion buffer (1 mg Collagenase D (Roche) and 0.2 mg DNase I (Roche ...
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bioRxiv - Microbiology 2023Quote: Cells were washed in PBS and harvested in lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris-HCl, protease inhibitor (Roche complete mini EDTA-free protease inhibitor) ...
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bioRxiv - Microbiology 2022Quote: Lungs were perfused with sterile PBS and digested at 37°C for 1 h with 625 μg/mL collagenase D (Roche) and 75 U/mL DNase I (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with ice-cold PBS and scraped into lysis buffer (1 mM EPPS, 8 M urea, protease (complete mini, EDTA-free) inhibitors (Roche) and PhosSTOP phosphatase inhibitor (Roche)) ...
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bioRxiv - Neuroscience 2023Quote: ... Five P2 mouse brains were homogenized in 5 mL of homogenization buffer (5 mM Tris-HCl, 0.32 M sucrose, 1 mM MgCl2) supplemented with EDTA-free protease inhibitors (cOmplete™, Roche), then centrifuged at 1,660 x g for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Whole kidneys were homogenized and protein was extracted from 20 mg of tissue in 1 ml RIPA buffer supplemented with protease inhibitors (Roche) for 2 hrs ...
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bioRxiv - Cancer Biology 2022Quote: Frozen bacteria pellets were thawed on ice and loosened in 25 mL lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X100, 10 mM DTT, 1X Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pelleted, resuspended in HSB (PBS supplemented with 150 mM NaCl, 1 mM DTT, protease inhibitor cocktail Complete (Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once with PBS and resuspend with Tris-HCl containing 4 mM EDTA and 1× complete protease inhibitor cocktail (Roche). Dounce homogenizer is used to fractionate the cell membranes and then 3 min 600 g centrifugation was used to obtain Post-Nuclear Supernatants (PNS) ...
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the protein A fragment of the TAP tag or anti-HA peroxidase 1/500 (clone 3F10, Roche).
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bioRxiv - Immunology 2022Quote: ... Following 79 μL of 50 mM Tris-HCl was added together with 1 μL of 100x protease inhibitor cocktail (Roche) before the probe sonicated with VibraCell probe (Sonics & Materials ...
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bioRxiv - Molecular Biology 2023Quote: Thawed and washed cell pellets were lysed in 1 mL sterile PBS with 10% (v/v) glycerol and 1x cOmplete mini protease inhibitor cocktail (Roche). Ten micrograms of protein were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 1 ml of the buffer required for each particular application (see below) and supplemented with protease inhibitors (Roche), and then disrupted by sonication ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Neuroscience 2023Quote: ... 2 mM MgCl2 and 1 mM DTT supplemented with DNase I (10 µg/ml) and complete protease inhibitor cocktail tablets (Roche). Complex formation was initiated by adding synthesized Stachel peptide (100 µM ...
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bioRxiv - Pathology 2023Quote: ... Spain) (1:10000) for 1.5h in darkness was followed by the addition of HRP substrate (11112422001, Roche Applied Science, Germany). Absorbance was measured using NanoQuant Infinite M200 Pro multi-plate reader (Tecan ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in PBS for 30 min and incubated overnight at 4°C with 1:1,500 anti-HA high affinity (Roche, 3F10). After three PBS washes ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR was set up as a 10μL reaction using SYBR® Green 1 480 Lightcycler® Master (Roche) in a 384-well plate ...
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: HK cells were lysed using a lysis buffer (50mM Hepes, 130mM NaCl, 1mM DTT 1% NP-40) with complete protease inhibitor cocktail (Roche). Cell lysates were centrifuged at 13,000 rpm for 10 mins at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant was centrifuged at 20000 g for 30 min at 4°C and the resulting pellet was solubilized with lysis buffer (20 mM Tris–HCl pH 8, 150 mM NaCl, 1% TritonX100, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then subjected to a transcardial perfusion with calcium-free Tyrode’s solution containing protein phosphatase-inhibitors (+PPIs) (1 tablet PhosSTOP per 10 ml) (Roche 04906845001) followed by Zamboni’s fixative containing 4% paraformaldehyde and 0.2% picric acid in 0.1 M phosphate buffer pH 7.4 +PPIs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Slides were incubated with 50 μL of block buffer containing a fluorescein-labeled anti-DIG antibody (sheep, 1:100, Roche) for 60 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: RPE-1 cells were trans-fected with the different Flag constructs for 48 h using X-tremeGENE 9 DNA reagent (Roche). Cells were lysed in 25mM Tris (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 1 ml hypotonic buffer supplemented with 10 µg/ml PMSF and protease inhibitor cocktail (Roche). Cells were incubated on ice for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... For subsequent PCR amplification of the cDNA the optimal PCR cycle number was determined with an aliquot of 1 μL unpurified cDNA in a 10 μL qPCR containing 1X Kapa HiFi Hotstart Readymix (Roche), 1x SybrGreen and 0.2 μM UP primer (Table S2) ...
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bioRxiv - Cell Biology 2023Quote: ... IPTG-treated bacteria were washed with PBS and eluted with 1X HEPES buffer containing 1% Triton X-100 and EDTA-free protease inhibitor cocktail (Roche) The elutes were sonicated and centrifuged at 18,000 x g ...
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bioRxiv - Microbiology 2023Quote: ... and lysed on ice using a lysis buffer (PBS, 0.1% SDS, 1% Triton X-100, EDTA-free complete protease inhibitor (Roche Diagnostics)) ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 30 ml of Solution A supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 0.175x cOmplete™ (Mini, EDTA-free) protease inhibitor cocktail (Roche) in a 50 ml Falcon tube and disrupted by vortexing with an equal volume of glass beads (425-600 μm ...
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bioRxiv - Biochemistry 2023Quote: ... SRA01/04 cell lysates were obtained using RIPA lysis buffer (Beyotime) supplemented immediately before use with 10 μl ml-1 phosphatase and protease inhibitors (PhosSTOPTM, Roche). The bicinchoninic acid assay (Beyotime ...
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bioRxiv - Genetics 2023Quote: ... Phosphorylation of Sch9 was monitored by western blotting probed with anti-HA 3F10 antibody (1:2000; Roche Life Science, USA) followed by goat anti-rat HRP-conjugated antibody (1:5000 ...