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Citations for Roche :
501 - 550 of 837 citations for Mouse IRX6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Digxigenin (DIG)-labeled sense and antisense probes were performed from the linearized pGEM-T-easy plasmids using the DIG RNA Labeling Kit (Roche).
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bioRxiv - Developmental Biology 2020Quote: ... gbx1 and pax6a digoxigenin and FITC antisense probes were generated from linearized plasmids using an RNA labelling and detection kit (Roche)87 ...
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bioRxiv - Plant Biology 2022Quote: ... The ELVd probe was obtained via in vitro transcription of a linearized plasmid for 1 h at 37°C with 20 U of T3 bacteriophage RNA polymerase (Roche) in 40 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Molecular Biology 2020Quote: ... were generated by transfection of 3×FLAG-tagged LRRK2 (WT) plasmid followed by pharmacological selection using an antibiotic G-418 (Roche). Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... thermophila intron were obtained by in vitro transcription of the corresponding linearized plasmids with 20 U of T3 bacteriophage RNA polymerase (Roche) in 20-μl reactions containing 40 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2019Quote: ... Antisense DIG-labelled eiger RNA probe was prepared from XhoI-linearized pBSK-eiger plasmid using T3 RNA polymerase (DIG RNA Labeling Kit, Roche) and detected with the DIG Nucleic Acid Detection Kit (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... and the next day a total of 1 μg per well of a 1:1 mix of X22B and X22C CRISPR plasmids (either pX459 or pX462) was transfected by use of X-tremeGENE HP DNA transfection reagent (Roche). The next day ...
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bioRxiv - Developmental Biology 2019Quote: ... Both sense and anti-sense RNA probes were synthesized from restriction enzyme linearized plasmids transcribed with T7 or SP6 polymerase in the presence of RNA DIG labelling mix (Roche). Newly synthesized probes were purified using mini QuickSpin columns according to the manufacturer’s protocol (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... the cells became 70-80% confluent and were transfected with the corresponding plasmids using the transfection reagent FuGENE 6 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
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bioRxiv - Bioengineering 2020Quote: ... The corresponding fragments were inserted in miRVec expression plasmid (restricted with the same enzymes) by ligation with T4 ligase (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were first infected with plasmids bearing the hygromycin selection cassette followed by selection with 0.25 mg/ml hygromycin (Roche, 10843555001) for one week ...
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bioRxiv - Molecular Biology 2021Quote: ... in 48-well plates and transfected at 50-60% confluence with a total of 100 ng Dual-pMIR-report plasmids using X-tremeGENE 9 DNA Transfection Reagent (Roche), followed by transfection with mimics (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: Aag2 cells were transfected with a plasmid expressing 3×flag tagged Zuc using X-tremeGENE HP DNA Transfection Reagent (Roche), and fixed 48 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: A 3×flag tagged Zuc expression plasmid was transfected into Aag2 cells using X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were lysed and lysates incubated with M2-Flag beads (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... high specific-activity RNA probes were produced from a linearized plasmid (HindIII) and labeled using the Fluorescein RNA Labeling Mix (Roche), following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was produced in HEK293T cells using helper plasmids (VSVG and psPAX2) with X-tremeGENE 9 DNA Transfection Reagent (Roche). Target cell lines were infected with the virus and 10 μg/ml polybrene ...
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bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
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bioRxiv - Genetics 2019Quote: ... 1 ug purified digested plasmid was used as template for SP6 or T7 transcription using the DIG-RNA labeling kit (Roche). Following probe transcription ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were transfected with 1 μg Kras or control expression plasmid and 1 μg of viral packaging plasmids (250 ng pMD2.G and 750 ng psPAX2) using 6 μl of X-tremeGENE 9 transfection reagent (Roche). After 24 hours of transfection ...
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bioRxiv - Genomics 2019Quote: We amplified the Tug1 cDNA sequence with primers (see Sequences and Primers below) having KpnI and NotI restriction enzyme overhangs from the pTRE2-Tug1 vector plasmid using Q5 polymerase (Roche) and under following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... Handles were PCR-generated from the plasmid pSP73-JY0 (Fili et al., 2010) using appropriate oligonucleotides (Supplementary Table S1) and Bio-dUTP or Dig-dUTP (Roche), followed by restriction with PsiI or KpnI ...
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bioRxiv - Genomics 2019Quote: ... the cells were transfected with 10 μg of the plasmid libraries (HSS, ORI, and pGL4) using X-tremeGENE HP (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections and antibody staining were performed as follows: two micrograms plasmid DNA was transfected using Xtremegene HP transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were transfected with 1 ug of shRNA/cDNA and 1 ug of viral packaging plasmids (250ng pMD2.G and 750ng psPAX2) using 6 ul of Xtreme Gene 9 transfection reagent (Roche). After 24 hr ...
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bioRxiv - Genetics 2021Quote: Overexpression plasmids were respectively transfected into U251-APP cells by using the X-tremeGene HP DNA transfection reagent (ROCHE, 6366236001). Cells grown in 6-well plate were transfected by overexpression plasmid of the target gene or its corresponding control plasmid (each 2 μg ...
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bioRxiv - Biochemistry 2021Quote: For transient transfection cells were seeded and after 18 h cells were transfected with plasmid DNA using X-tremeGENE HP DNA transfection reagent (Roche), according to the manufacturer’s protocol (for 12-well plate format ...
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bioRxiv - Biochemistry 2021Quote: ... and pPB-tetIRB-tetON-BSD plasmid bearing CLPTM1L-mEGFP or pPB-tetON-BSD-tightFF plasmid bearing CLPTM1L-FLAG-6His using X-tremeGENE 9 DNA transfection reagent (Roche), and incubated at 37 °C for 2 days ...
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bioRxiv - Biochemistry 2021Quote: ... 2×106 cells HEK293T cells were transfected with 2 μg plasmid DNA using X-tremeGENE HP DNA Transfection Reagent (Roche) in 10-cm dishes ...
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bioRxiv - Bioengineering 2020Quote: ... Hela cells were plated one day before in 6-well plate at 50% confluency and transfected with 1 μg plasmid and 3 μl XtremeGeneHP transfection reagent (Roche) during 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were digested in 50-100 μl total volumes with restriction enzymes and their appropriate buffer (ether Roche or NEB) in accordance with the manufacturers recommendations (typically 1 μg of DNA was digested with 1 unit of enzyme for 1 hour at 37°C) ...
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bioRxiv - Microbiology 2021Quote: ... and transfected with plasmid pIIIH5red-SARS-2-S DNA using X-tremeGENE HP DNA Transfection Reagent (Roche Diagnostics, Penzberg, Germany) according to the manual ...
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bioRxiv - Developmental Biology 2021Quote: PCR products were cloned in pBlueScript KS+ vector and digoxigenin-labelled probes were generated from linearized plasmids using RNA-labeling mix (Roche). Whole-mount chromogenic in situ hybridization was performed as described previously (Marchal 2009 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then transiently transfected with MaMTH bait (MMD or MBOAT7) and prey (MMD, MBOAT7 or PEX7 negative control) plasmids using X-tremeGENE9 DNA transfection reagent (Roche) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Both Caco2 and HepG2 cells were transfected with 1 µg of pooled plasmid using X-tremeGENE9 Transfection Reagent (Roche:6365779001) following manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche, 6366236001), at a ratio of 400 ng DNA to 40 µL 37 °C serum-free DMEM to 1.6 µL reagent per dish being transfected ...
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bioRxiv - Neuroscience 2022Quote: ... Antisense and sense RNA probes were synthesized from the linearized plasmid template by in vitro transcription using a DIG RNA Labeling Kit (Roche). In situ hybridization was performed on cryo-sections of telencephalon as previously described (Masai et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... grown for 24 hours and transfected with 8 μg of plasmids and 8 μl of X-tremeGENE HP DNA Transfection Reagent (Roche). The cells were grown for 48 hours post-transfection and collected for downstream analyses.
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bioRxiv - Developmental Biology 2023Quote: ... Purified plasmids were used to prepare digoxigenin-labelled sense and anti-sense riboprobes using T7 and SP6 RNA polymerases (Roche). Probes were purified using ethanol precipitation and resuspended in 1:1 volume of DEPC-treated water:formamide.
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bioRxiv - Bioengineering 2023Quote: ... The construct was synthesized by GenScript® and the plasmid was used as template for PCR amplification (Kapa Hifi Hotstart Ready Mix, Cat#F-530-L, Roche). The PCR amplicon was purified with NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel ...
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bioRxiv - Cell Biology 2023Quote: Parental and Haspin knockout cells stably expressing GFP-TAF5 were obtained by cotransfection with pcDNA5/FRT/TO/GFP-TAF5 (Antonova et al., 2018) and a pPGKpuro resistance plasmid (in a 9:1 ratio) using Xtreme Gene 9 (Roche). After 48 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1μg linearized plasmid was used for In Vitro transcription to make DIG labelled RNA probe (DIG RNA Labeling Mix-11277073910 Roche). Whole mount RNA in situ hybridization was performed by following the standard zebrafish protocol (http://zfin.org/ZFIN/Methods/ThisseProtocol).
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bioRxiv - Developmental Biology 2022Quote: ... Plasmids were linearized and probes were prepared as described in Wilkinson & Nieto (1993) using digoxigenin-conjugated nucleotides (Roche, Basel, Switzerland). Sense probes were used to confirm specificity of the antisense probes ...
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bioRxiv - Cell Biology 2024Quote: ... RPE1 cells were transfected with PB-Tet-LAP-MAP3K1 and PB-Transposase plasmid (kindly provided by N. Dimitrova) using X-tremeGENE9 reagent (Roche) and after 48 hours cells were selected with G418 ...
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bioRxiv - Microbiology 2023Quote: ... a 2.3 kb-homologous DNA fragment was cloned into a unique SpeI site of a non-replicative plasmid (pIJ10700) using T4 DNA ligase (Roche). The fragment was amplified using Q5® DNA polymerase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293T and MEF cells (except for p62 KO MEF cells) were transfected with plasmids using X-tremeGENE 9 (Roche) as described as previously (41–43) ...
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bioRxiv - Genomics 2024Quote: ... Cells were then transfected with the Tet-pLKO-puro plasmids targeting either UHRF1 or DNMT1 transcripts and the accompanying lentiviral packaging (psPAX2) and envelope (pMD.2G) plasmids with Opti-MEM and Xtreme HP Gene transfection reagent (Roche) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with 1 µg of shRNA/cDNA and 1 µg of viral packaging plasmids (250 ng pMD2.G and 750 ng psPAX2) using 6 µl of Xtreme Gene 9 transfection reagent (Roche). After 24 hours of transfection ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then incubated with mouse anti-GFP monoclonal antibody(1:1000; Roche, Indianapolis, IN) as well as rabbit anti-alpha-tubulin polyclonal antibody (1:1000 ...