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Citations for Roche :
5401 - 5450 of 6468 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Large scale preps of bacteria cultures containing the sgRNA plasmid library were harvested using the Genopure plasmid maxi kit (Roche). SIDP library was packaged in lentiviral particles by large scale co-transfection of HEK293T cells with CELLECTA ready-to-use packaging plasmid (Cellecta – cat.no CPCP-K2A ...
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bioRxiv - Microbiology 2022Quote: ... and by quantitative PCR (qPCR) using a KAPA Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, Roche, Wilmington, MA). Samples were multiplexed and sequenced with a 15% spike-in of PhiX DNA in an Illumina NovaSeq platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and by quantitative PCR (qPCR) using a KAPA Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, Roche, Wilmington, MA). Samples were multiplexed and sequenced with a 15% spike-in of PhiX DNA in an Illumina NovaSeq platform (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μm sections were stained with H&E and Masson trichrome (Trichrome II Blue staining kit at Nexus special stainer; Roche). Immunohistochemical analysis was performed on cryosections and paraffin sections using a Bench Mark XT automatic staining platform (Ventana ...
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bioRxiv - Genetics 2022Quote: ... were prepared and sequenced by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA mRNA HyperPrep kit (v4.17) (Kapa Biosystems). RNA was sheared to 300-400 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... genomic (g)DNA was extracted both from primary Irf4−/− tumours as well as FACS-sorted control B220+mIgM- BM fr.A-D cells using the High Pure PCR Template Preparation kit from Roche (11796828001). Integrity of resultant gDNA was confirmed in a 2 % Agarose gel ...
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bioRxiv - Molecular Biology 2022Quote: Cytotoxicity was determined as lactate dehydrogenase (LDH) activity in cultured supernatants using a cytotoxicity detection kit (#11644793001; Roche, Mannheim, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... DNase-treated RNA was converted into barcoded polyadenylated mRNA libraries using the Kapa Hyper Stranded mRNA Sample Prep Kit (Roche). Libraries were barcoded with unique dual indexes to prevent index switching and adaptor-ligated double-stranded cDNAs were PCR-amplified for eight cycles with KAPA HiFi DNA Polymerase (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, no. KK4610). Relative expression levels were normalized to human ACTB or mouse Actb ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Library quality was assessed using an Advanced Analytical Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Roche). Sequencing has been performed on a NovaSeq6000 S4 lane (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche). 1:5 diluted cDNAs was used for qPCR by using KAPA SYBR® FAST qPCR Master Mix (5X ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with RCAS-hPDGFB-HA and RCAS-shRNA-p53-Rfp using a Fugene 6 transfection kit (Roche, 11814443001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability analysis by lactate dehydrogenase (LDH) release assay was performed with the Cytotoxicity Detection Kit (LDH) (Roche, Mannheim, Germany) and has also been described 23,31.
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bioRxiv - Microbiology 2020Quote: ... Library concentrations for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Genomics 2021Quote: ... using 400 ng of total RNA from each sample for poly(A) mRNA capture followed by stranded mRNA-seq library construction using KAPA stranded mRNA-seq kit for Illumina (Roche) as per manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... Final libraries with different sample indices were pooled in equimolar ratios based on quantification using KAPA library quantification kit for Illumina platforms (Roche) and size analysis on Fragment Analyzer (AATI ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons were purified with 0.8x AMPure XP beads and 100ng of DNA was converted to paired-end Illumina sequencing libraries using the KAPA HyperPlus library preparation kit (Roche) with the KAPA unique dual-indexed adapters (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR optimisation was carried out on the full-length cDNA using the KAPA HiFi PCR kit (Kapa Biosystems, Boston USA) and 12 cycles was sufficient to generate the material required for SMRTbell library preparation ...
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bioRxiv - Microbiology 2019Quote: One-step simplex real-time quantitative RT-PCR amplifications were performed using Multiplex RNA Virus Master Kit (Roche Diagnostics, France) for influenza A ...
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bioRxiv - Plant Biology 2021Quote: ... Relative transcript abundancies were determined using the Roche LightCycler 480 and the LC480 SYBR Green I Master Kit (Roche Diagnostics). The resulting cycle threshold values were converted into relative expression values using the second derivative maximum method and ACTIN2 ...
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bioRxiv - Molecular Biology 2020Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Plant Biology 2021Quote: ... 50 ng of DNA was subjected to PCR amplification using the Kapa HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, USA). The individual PCR reactions were performed in 20 μL final volume and contained:
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bioRxiv - Microbiology 2021Quote: ... Between 150 and 200 ng of genomic DNA was sheared acoustically and HiSeq sequencing libraries were prepared using the KAPA Hyper Prep Kit (Roche). PCR amplification of the libraries was carried out for 10 cycles ...
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bioRxiv - Neuroscience 2021Quote: ... and quantitative real-time PCR was performed using the LightCycler DNA Master SYBR Green I Kit in a LightCycler 480 System (Roche). Oligonucleotides used for quantitative real-time OCR are listed in supplemental table S2 ...
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bioRxiv - Microbiology 2020Quote: ... whole specimens were processed into head/thorax homogenates for RNA extraction with the High Pure Viral Nucleic Acid Kit (Roche), according to manufacturer’s instructions (Moreira et al. ...
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bioRxiv - Immunology 2021Quote: ... 25-100 ng of total RNA was used to deplete rRNA using DNA probes and RNase H provided in RiboErase (HMR) kit (Roche). The depleted RNA was fragmented prior to cDNA synthesis followed by Illumina adaptor ligation using KAPA RNA HyperPrep kit (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was hybridized with digoxigenin-labeled DNA probes synthesized with a PCR DIG probe synthesis kit (Roche Molecular Biochemicals) as recommended by the supplier ...
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bioRxiv - Cancer Biology 2021Quote: ... Stranded RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) and KAPA Unique Dual-Indexed Adapters (Roche) according to instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and 100 ng of DNA was converted into paired-end Illumina sequencing libraries using KAPA HyperPlus library preparation kit (Roche) with the KAPA unique dual-indexed adapters (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNAseq libraries were prepared from 1 μg of total RNA per sample using the KAPA Stranded mRNA Sequencing Kit (Roche). In short ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplification was performed with the LC FastStart DNA master SYBR green I kit in a LightCycler 1.2 instrument (Roche Diagnostic) using the following specific primers ...
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bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
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bioRxiv - Microbiology 2021Quote: ... and used to generate the sequencing libraries using the KAPA Stranded mRNA Seq kit for the Illumina sequencing platform according to the manufacturer’s protocol (KAPA biosystems). Libraries were sequenced on an Illumina HiSeq 2500 in the Rapid Run mode using 100 nt single read sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
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bioRxiv - Neuroscience 2022Quote: ... to estimate the quantity and validate the size distribution and were then quantified by qPCR using the KAPA Library Quantification Kit KK4835 (# 07960204001, Roche) prior to the amplification with Illumina’s cBot ...
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bioRxiv - Cancer Biology 2022Quote: ... 500-1000 ng of sheared DNA was used as a template for a 6-cycle PCR to construct a fragmented library using the KAPA HTP Library Preparation Kit (Roche). Exome enrichment was performed using SeqCap EZ Enrichment Kit (Roche ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The sheared fragments were treated with end repair and A-tailing using the KAPA-Illumina library creation kit (KAPA biosystems) followed by immobilization of mate pair fragments on strepavidin beads (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was performed using an Eva Green 2X qPCR MasterMix-No Dye kit (Abm, MasterMix-S) on a LightCycler 480 instrument (Roche). Relative gene expression was analyzed based on the 2-ΔΔCt method with β-actin as internal controls ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with the LightCycler 480 SYBR Green I Master kit (Cat. 04887352001, Roche). Primers for pmp-3 and ACTIN were used as housekeeping for C ...
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bioRxiv - Cancer Biology 2022Quote: Viability and cell growth ratios were determined analyzing cell density by MTT using the Cell Proliferation Kit I (Roche, 11465007001) in DND41 cells treated with EX-527 (10-6 to 10-3 M ...
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bioRxiv - Cell Biology 2022Quote: ... the RNA concentration was determined and 1 µg RNA was used for the cDNA synthesis according to the Transcriptor First Strand cDNA Synthesis kit (Roche). The expression of the following genes FTSH7 (AT3G47060) ...
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bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s protocol. Quantitative PCR was performed using SensiMixTM II Probe Kit (Cat#. BIO-83005) with Universal ProbeLibrary (Roche Diagnostics), and the CFX96 Touch System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... The KAPA Stranded mRNA Sample Preparation Kit was used to prepare polyA enriched RNA libraries (Kapa Biosystems, Wilmington, MA, USA). Trimmomatic [73] (version 0.36 ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR products were then purified and reverse transcribed with digoxigenin labeling using a DIG RNA labelling kit (Roche, Switzerland). For dissected adult pituitary or 5-8 dpf larvae ...
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bioRxiv - Genomics 2019Quote: ... The dry contents were resuspended by addition of 7.5 µl hybridization buffer and 3 µl hybridization component A (SeqCap EZ Hybridization and Wash Kit: 05 634 261 001, Roche), mixed by tapping ...
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bioRxiv - Genomics 2019Quote: ... per sample were washed twice with 200 µl 1x wash buffer (SeqCap EZ Hybridization and Wash Kit: 05 634 261 001, Roche), once with 100 µl ...
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bioRxiv - Genomics 2019Quote: ... Non-target DNA was removed through washing the beads with buffers of the SeqCap EZ Hybridization and Wash Kit (05 634 261 001, Roche) as per manufacturers instructions with the following modifications ...
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bioRxiv - Biochemistry 2019Quote: ... and S278E amino acid substitutions were introduced into human RACK1 using the QuickChange II XL site-directed mutagenesis kit (Roche). All plasmids are available upon request.
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bioRxiv - Evolutionary Biology 2019Quote: ... Libraries were subsequently index-amplified for 20 cycles using a KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, Ma. USA) in 50-μl reactions following the manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2019Quote: We prepared sequencing libraries for the ten de novo sequenced isolates using the Kapa Biosystems kit (Kapa Biosystems, Woburn, MA) and ~1μg of DNA of each isolate according to manufacturer’s protocols ...