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Citations for Roche :
5201 - 5250 of 6468 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from the FFPE tissue by use of the High Pure FFPE RNA Micro Kit (Roche 04823125001). Slides were cut into 2 10 micron sections ...
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bioRxiv - Cancer Biology 2023Quote: ... One hundred nanograms of total RNA was used for library preparation with the KAPA mRNA Hyperprep Kit (KK8581) (Roche). Each resulting in a uniquely dual-indexed library was quantified and quality accessed by Qubit and TapeStation ...
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bioRxiv - Genomics 2023Quote: ... the final library was checked for quality and quantity using the Bioanalyzer and a KAPA Quantification Kit (Roche, USA), respectively ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmid backbones and DNA fragments were amplified using the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Boston, USA) and the primers described in Table S3 ...
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bioRxiv - Cell Biology 2023Quote: ... The TUNEL assay was performed using the in situ Cell Death Detection Kit (11684817910, Roche Applied Science, Penzberg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: RNA sequencing libraries were prepared with Kapa RNA mRNA HyperPrep kit (Kapa Biosystems, Cat KR1352; Cape Town, South Africa) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: NGS libraries from DRIP DNA and inputs were constructed according to the manufacturer’s instructions (KAPA Hyper Prep Kit: Roche, cat ...
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bioRxiv - Developmental Biology 2023Quote: ... Livers were then dissected out of the larvae and subjected to TUNEL assay according to manufacturer’s instructions (In Situ Cell Death Detection kit, Fluorescein; Roche). Nuclei were counterstained with Hoechst (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... of the VH gene was subjected to second-strand synthesis in a 25 μl reaction volume using IGHV gene-specific primers (primer No. 23–32, 7.5 μl) and a KAPA Biosystems kit (Roche). The reaction conditions were as follows ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and long-read (Nanopore) platforms: the Illumina library was prepared using the Kapa HyperPrep kit (Kapa Biosystems, Wilmington, MA). A-tailing ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (3–4 μg) was used to prepare cDNA with the Transcriptor First-Strand cDNA synthesis kit (Roche). qPCR was performed with TaqMan gene expression assay primers (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Library quality control and quantification were performed using a KAPA Library Quantification kit for Illumina platforms (Kapa Biosystems, KK4873) and a 2100 Bioanalyzer High Sensitivity DNA kit (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using a One-step BrightGreen RT-qPCR Kit (Abm, G471-S) following the manufacturer’s protocol in the LightCycler 96 Real-Time PCR System (Roche). Isocitrate dehydrogenase (ICDH ...
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bioRxiv - Microbiology 2023Quote: Sequencing was done using Illumina HiSeq X with sequencing shotgun library preparation using the KAPA HyperPrep Kit (Kapa Biosystems) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... TUNEL staining was performed as in (46) using the TMR red in situ cell death detection kit (Roche, 12156792910).
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (1Lμg) was used to generate RNA-seq library with KAPA HyperPrep RNA Kits with RiboErase (Roche KK8560) using 8 cycles of PCR amplification ...
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bioRxiv - Systems Biology 2023Quote: ... RNA libraries were prepared for paired-end sequencing using the KAPA Stranded RNA-Seq Library Preparation kit (Kapa Biosystems). Libraries were sequenced on a NextSeq system (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Developmental Biology 2023Quote: ... The molarity of adaptor-modified molecules was defined by a qPCR with a KAPA Library Quantification kit (Kapa Biosystems). Individual libraries were normalized to 10nM ...
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bioRxiv - Genomics 2023Quote: Carver Biotechnology Center at the University of Illinois at Urbana-Champaign with the Hyper Library Construction Kit (Kapa Biosystems) to obtain an average library fragment size of 500bp ...
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bioRxiv - Microbiology 2023Quote: ... The PCR DIG Probe Synthesis kit for probe synthesis and the CSPD luminescent detection kit for visualization of the bound probe were used according to the manufacturer’s instructions (Roche Molecular Biochemicals ...
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bioRxiv - Immunology 2023Quote: ... Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete, Universal) (Kapa Biosystems, Woburn, MA). Pooled libraries were sequenced on an Illumina NovaSeq 6000 using 150bp PE reads (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... we performed target-capture using a custom-designed probe kit with a SeqCap EZ Workflow (Roche, Indianapolis, IN, USA), which was designed to capture the entire nuclear genome of S ...
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from A549 cells grown in 6-well plates was done using High Pure RNA isolation kit (Roche). Transcriptor First strand kit (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of each captured library pool was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (Roche) to produce cluster counts appropriate for the Illumina NovaSeq-6000 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... The ribodepletion method of library preparation for RNA sequencing was performed using the Total-RNASeq RiboErase Kit (KAPA Biosystems). All RNA samples were loaded two separate 2-lane flow cells and sequenced using an Illumina HiSeq 2500 system generating 51 bp paired-end reads ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 20 x 106 cells were used for isolating genomic DNA with High Pure PCR Template Preparation Kit (Roche) as described by the manufacturer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche, Basel, Switzerland) with adaptor ligation and adaptor fill-in reactions based on Meyer and Kircher (2010) ...
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bioRxiv - Genomics 2023Quote: ... Adapter-ligated material was amplified with 12-14 cycles of PCR using KAPA HiFi HotStart PCR Kit (Roche, 07958897001) and universal P5/P7 Illumina primers (see Golov et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... We synthesized digoxigenin (DIG)-labeled sense and antisense RNA probes using a DIG RNA Labeling Kit (Roche, Basel, Switzerland) after digestion with the appropriate restriction enzymes BamHⅠ-HF ...
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bioRxiv - Physiology 2023Quote: ... Cell apoptosis was measured using an in situ cell death detection kit and following the manufacturer protocol (Roche, 12156792910). Images were obtained using an inverted fluorescent microscope (Leica DMi8 ...
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bioRxiv - Cell Biology 2023Quote: MTT activity was determined using the Cell Proliferation Kit I according to the recommendations of the manufacturer (Roche, Spain). Optical density was determined at 575[nm with a reference wavelength of 690[nm using a Varioskan Flash spectrophotometer (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Genotyping of ESCs and mouse tails were performed by PCR using KAPA Mouse Genotyping Kit (Roche, Cat. No. KK7302). Specific protocol followed the reagent instructions ...
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bioRxiv - Immunology 2024Quote: ... Adapter ligation and indexing PCR was performed on these amplicons using KAPA HTP Library Preparation Kit (KK8234, Kapa biosystems) for multiplexing and sequencing on an Illumina HiSeq5 platform.
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from A549 cells under different treatment conditions using the High Pure RNA Isolation Kit (Roche), following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... 100 nanograms of total RNA were used for library preparation with the KAPA total RNA Hyperprep Kit (KK8581) (Roche). Each resulting uniquely dual-indexed library was quantified and quality accessed by Qubit and Agilent TapeStation ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluted mRNA was then reverse transcribed using random hexamer primers and Transcriptor First Strand cDNA Synthesis Kit (#04897030001, Roche) at 65°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 μg of total RNA was retro-transcribed using Transcriptor First Strand cDNA Synthesis Kit (Roche; Cat #04897030001) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... The U6-sgRNA cassette was then amplified by PCR using the KAPA HIFI Hot Start PCR kit (Roche, KK2502) and different tagged primers required for the subsequent Illumina sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... Cell death was evaluated separately using the Fluorescence In Situ Cell Death Detection kit (Roche Diagnostic, Indianapolis, IN, US). Brain sections were analyzed for DNA strand breakage using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries for RNA-seq were prepared with KAPA Stranded mRNA-Seq poly(A) selected kit (KAPA Biosystems, Wilmington, MA) using 250 ng toal RNAs for each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems, #KK8504) using 8 cycles of PCR ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were constructed from approximately 100 ng of genomic DNA using the Kappa HyperPrep Kit (Roche, Pleasanton, CA, USA) with mechanical shearing (Covaris ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was purified with High Pure Viral Nucleic Acid Kit according to the manufacturer’s instruction (ROCHE, Mannheim, Germany). Viral RNA was amplified by LightMix® Modular Sarbecovirus E-gene (Roche ...
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bioRxiv - Immunology 2024Quote: ... adhering to the manufacturer’s recommendations for low-input DNA (MyBaits v5 kit Arbor Biosciences) and using KAPA Universal Enhancing Oligos ((Roche) to prevent non-specific binding ...
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bioRxiv - Genomics 2024Quote: Total RNA and DNA:RNA hybrid samples were reverse transcribed into complementary DNA (cDNA) using Evoscript universal cDNA Master Kit (Roche, Germany ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin labeled antisense riboprobes were synthesized by in vitro transcription using a DIG RNA labeling kit (Roche, Cat#11277073910), followed by purification using Micro Bio-Spin P-30 gel columns (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: The labeling of probes with digoxigenin was performed using the 2nd generation DIG Gel Shift EMSA kit (Roche, USA), following the instructions provided by the manufacturer ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA fragmentation was visualized by using an in situ cell death detection kit (Fluorescein; Cat #, 11684795910; Roche, Basel, Switzerland) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...