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Citations for Roche :
5301 - 5350 of 6311 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: All the samples were lysed with high-salt buffer (50 mM Tris pH 7.5, 300 mM NaCl, 1% Triton-x-100, protease inhibitors (11836170001, Roche), and Pierce Phosphatase inhibitor cocktail (A32957 ...
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bioRxiv - Plant Biology 2024Quote: ... Plant tissues were ground in liquid nitrogen and the powder was suspended in lysis buffer (50 mM Tris-HCl at pH 8.0, 150 mM NaCl, 1 mM phenylmethanesulfonyl fluoride, 10 mM iodoacetamide, and 1X Roche cOmplete mini protease inhibitor cocktail) ...
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bioRxiv - Genetics 2024Quote: ... The amplification PCRs (Primers 1-6 Table S7) were done with Kapa HiFi HS Ready Mix (Kapa Biosystems 07983034001), using 20 ng input template and 50 ul final volume except for the High GC bin in which 20 ul of KAPA2G GC Buffer (ROCHE KK5516 ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0 and 1 %) complemented with complete protease inhibitor cocktail (PIC) (Complete EDTA-free Proteinase inhibitor cocktail tablets (11836170001, Roche)) ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated in blocking solution containing 5% horse serum and 1% Western Blocking Reagent (Roche Diagnostics, Mannheim, Germany) in TNTx for at least 2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... protease inhibitor (1 tablet per 10 ml solution; cOmplete ULTRA Tablets-Mini, F. Hoffmann-La Roche AG, Basel, Switzerland) was added ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 4 ml of a buffer (HEPES 10 mM pH 7.4, 1 mM EDTA, EDTA-free protease inhibitor cocktail from a tablet (Roche)) and lysed by 3 passages through a cell disrupter at 2.4 kbar ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2) supplemented with a cocktail of protease inhibitors (CLAPA and 1 µg/mL cOmplete, EDTA-free, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Microbiology 2024Quote: ... Proteases were inactivated by the addition of 1 mM PMSF and 10 ul 1x complete protease inhibitor cocktail (Roche).
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bioRxiv - Neuroscience 2024Quote: ... cells were lysed in IPH buffer (20mM Tris-HCl pH7.5, 150mM NaCl, 2mM EDTA, 1% NP-40) supplemented with protease inhibitor cocktail (Roche, cOmpleteTM EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2024Quote: ... 1% NP-40 (vol/vol) supplemented with protease inhibitor cocktail (cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, Cat# 05892791001) for 20 minutes at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells are then washed twice in cold 1% BSA solution containing 0.5X EDTA-free Protease inhibitor cocktail (PIC, Roche), and once in cold PBS ...
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bioRxiv - Biochemistry 2024Quote: Cells were lysed in native lysis buffer (10% glycerol, 25 mM NaCl, 20 mM HEPES pH 7.0, 1% DDM) supplemented with protease inhibitor tablet (Roche), and solubilized by rotating 30 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with primary antibody for 2.5 h (GFP, Abcam, Cambridge, UK: rabbit, 1:10000; HA (Roche), rat ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated in 50% Hybridization Buffer (50% formamide, 2x SSC, 1 mg/ml Torula RNA, 0.05 mg/ml Heparin, 2% Roche blocking reagent ...
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bioRxiv - Physiology 2024Quote: ... cells were lysed in IP buffer (10 mM Tris pH 8, 0.4% NP40, 300 mM NaCl, 10% glycerol, 1 mM DTT, antiprotease (Roche), anti-phosphatase (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 1µg of DNase from bovine pancreas and 1 dissolved tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Pellets were then homogenized with a Biospec Tissue Tearor (Dremel) ...
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bioRxiv - Plant Biology 2024Quote: ... The powdered tissue was then mixed with 1 ml of lysis buffer per gram of tissue (150 mM NaCl, 0.1% Triton X, and 50 mM TRIS HCl-pH 8, supplemented with 13:1 of Complete Mini, Roche).
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bioRxiv - Plant Biology 2024Quote: ... Aggregate fractionation buffer (100 mM HEPES pH 7.4, 1% Triton X-100, 300 mM NaCl) supplemented with Protease Inhibitor Cocktail (Roche) and 25 mM N-ethylmaleimide was added at 3 volumes per unit mass of dried seedling weight ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were washed in HBSS and then dissociated using 1 mg/mL collagenase and dispase (Roche, Indianapolis, IN, USA) for 45 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was washed five times with 10 CV of Amylose Buffer (30 mM HEPES pH 7.6, 0.3 M NaCl, 0.1% CHAPS, 0.25 mM EDTA, 2 mM DTT, 10% glycerol, 1× Roche cOmplete protease inhibitors). Protein was eluted with Amylose Buffer supplemented with 20 mM maltose ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM pyridoxal 5’-phosphate hydrate (PLP) and proteases inhibitor cocktail (cOmplete™, EDTA-free Protease Inhibitor Cocktail Roche).
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bioRxiv - Molecular Biology 2024Quote: ... were pelleted and resuspended in NP-40 lysis buffer (150 mM NaCl, 1% NP-40, 50 mM Tris-Cl pH 8.0) containing cOmplete protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of RNA was converted into cDNA using the Transcriptor high-fidelity cDNA synthesis kit (Roche, Basel, Switzerland). The PCR mix contained 10 ng cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... The cell pellet was resuspended in Lysis buffer (20mM Tris-HCl pH 7.5, 150mM NaCl, 1% SDS, 1mM EDTA) containing protease inhibitor cocktail (Roche). The lysate was clarified by centrifugation (17,000xg for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... Brain stems were cut into pieces and digested for 40 min at 37 °C with 5% CO2 in PBS supplemented with 1.6 IU ml-1 Collagenase/Dispase (Roche) and 0.5 mg ml-1 DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... Tissues were then incubated for 1.5 hours at 37°C in an orbital shaker (200 rpm) with 1 ml of digestion media containing 0.25 mg/ml Liberase TL (Roche) and 0.25 mg/ml DNase I (Sigma ...
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bioRxiv - Immunology 2024Quote: ... brachial and mesenteric lymph nodes from 3 mice/genotype were pooled and cut into pieces and digested with the 1 ml of enzyme mix containing 0.8 mg/ml Dispase (Roche), 0.2 mg/ml Collagenase P (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were co-lysed together in lysis buffer (40mM Tris-HCl pH 7.5, 500mM NaCl and 1 mM DTT) with 100 mM Pefabloc (Roche) and protease inhibitor cocktail (Roche cOmplete EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was resuspended in lysis buffer (80 mL per L of initial culture: 20 mM HEPES pH 8, 500 mM NaCl, 5% v/v glycerol, 0.5 mM TCEP + 1 Roche cOmplete EDTA-free protease inhibitor tablet ...
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bioRxiv - Cancer Biology 2024Quote: ... and MAZ (1:25, Immunoway Cat# YT2669) was performed using the Ventana BenchMark ULTRA instrument (Ventana/Roche, AZ, USA) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... and the remaining cell pellet was lysed at a final concentration of 1.25×106 cells/mL in lysis buffer (1% NP40-PBS containing protease and phosphatase inhibitors (Roche)) and stored at -20°C until further analysis.
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 20 mM Imidazole, 1 mM TCEP, and protease inhibitors from Roche), and lysed by several passes through a microfluidizer (LM20 Microfluidizer ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were washed twice with cold PBS before resuspending in ChIP shearing buffer (50 mM Tris HCl pH 8.0, 1% SDS, 10 mM EDTA with protease and phosphatase inhibitors, Roche). Samples were sonicated using a Covaris ME220 for 7.5 minutes using the following sonication conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody-bound beads were washed in BSA-PBS (0.5%) before the addition of pre-cleared lysate in 1% Triton with protease inhibitor (Roche). Following rotation overnight at 4 °C the beads were washed in RIPA (50 mM HEPES pH 7.6 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and resuspended in lysis buffer (50 mM HEPES pH 8.0, 300 mM NaCl, 1 mM TCEP, supplemented with Roche EDTA-free cOmplete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2024Quote: ... islets were preincubated for 1 hour in KRBH buffer containing 0.1% (w/v) bovine serum albumin (BSA) (10775835001, Roche) and 3 mM glucose before incubation with 11 mM glucose ± 100 nM GLP-1RAs in KRBH buffer in a shaking 37°C water bath (80 rpm ...
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bioRxiv - Cancer Biology 2024Quote: Equal amounts of total RNA (1 µg) were reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with random hexamer primers for one hour at 50°C ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 3× protease inhibitor cocktail [Roche cOmplete]), and lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Biochemistry 2024Quote: ... and pellets were resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]). The cells were lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]), and lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Cell Biology 2024Quote: ... and thawing and by resuspension in 5 mL of ice-cold Lysis/Wash buffer (25 mM HEPES pH 7.5, 1 M NaCl, 5% glycerol) supplemented with cOmplete protease inhibitor cocktail (Roche), followed by vigorous sonication on ice delivered by a Jencons Ultrasonic Processor (15s bursts at 70% amplitude) ...
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bioRxiv - Cell Biology 2024Quote: ... The nuclear pellet was washed once with 3mL ST buffer and then twice with 3mL FANS buffer (1% BSA, 1mM DTT, and 0.2U/μL RNAse inhibitor (03 335 402 001, Protector RNAse inhibitor, Roche) in RNAse free DPBS (AM9624 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacteria pellets were resuspended in Lysis Buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM DTT, 0.1% Triton-X, and protease inhibitors, cOmplete Mini, EDTA-free, Roche, #4693159001). Next ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 100 mL of cold lysis buffer (50 mM HEPES pH 8, 175 mM NaCl, 5 % glycerol, 1 mM EDTA and 2 tablets of protease inhibitors by Roche). The bacterial solution was then incubated at room temperature (RT ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM Tris/HCl pH8) to which protease inhibitors were freshly added (1 tablet of Complete protease inhibitor per 25 ml, Roche, Mannheim ...
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bioRxiv - Cancer Biology 2021Quote: ... 150 mM NaCl, 1 mM PMSF, 1x protease inhibitor cocktail without EDTA from WAKO, 1x phosphatase inhibitor cocktail from Roche) containing 20 mM N-Ethylmaleimide to prevent further cysteine oxidation during protein extraction ...
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bioRxiv - Cell Biology 2020Quote: Cells were harvested using a cell scraper and were lysed with 200 μl/well of 0.1% CHAPS lysis buffer (50 mM Tris-HCl, 150 mM NaCl and 1 mM EDTA) supplemented with a Mini Protease inhibitor cocktail (Roche). The cell lysate was incubated on ice for 15 mins with occasional mixing before centrifugation at 20,000 g at 4°C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... The pellets were washed once with Buffer A and then further lysed with 250 μl modified RIPA buffer (1mM EDTA, 150mM NaCl, 0.1% Na-DOC, 1% NP-40, 50Mm Tris pH7.5 and Complete Roche protein inhibitors). 50U of benzonase (nuclease ...