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Citations for Roche :
5201 - 5250 of 6180 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with ice-cold DPBS and scraped in ice-cold kit lysis buffer (1 mL/107 cells) containing a cocktail of protease inhibitors (cOmplete, EDTA-free; Roche). Cells were then lysed in a Potter-Elvehjem homogenizer (~50 strokes) ...
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bioRxiv - Cell Biology 2019Quote: ... 200 mM NaCl; 1mM EDTA, pH 8.0; 0.5 % Nonidet P-40; 1 tablet complete protease inhibitor per 50ml, Roche, Mannheim, Germany). For each pulldown ...
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bioRxiv - Biochemistry 2021Quote: ... 150 mM sodium chloride (NaCl), 20% glycerol, and 1 mM Dithiothreitol (DTT, Scientific) supplemented with one Complete EDTA free protease inhibitor tablet (Roche) per 50 ml lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... S-phase Synchronous HeLa S3 cells were washed with ice-cold 1× PBS and lysed in a swelling buffer (20 mM HEPES, pH 7.5, 2 mM MgCl2, 5 mM KCl, 1 mM Dithiothreitol [DTT], and protease inhibitor cocktail [Roche; #11836170001]) supplemented with energy-regenerating mixture ...
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bioRxiv - Genetics 2019Quote: ... The cell pellet was resuspended in cold sonication buffer (50mM Tris-HCl pH8, 10mM EDTA, 1% SDS) supplemented with protease inhibitors (Complete, Roche), and chromatin was sheared to an average size of 200-500bp by sonication for 35 cycles (30s ON/30s OFF ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated with 0.5% blocking reagent in PBT before an overnight incubation in 1:2000 anti-dig AP antibody (Roche, 11093274910) in 0.5% blocking reagent (Roche ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Probed embryos were blocked for 1-2 hours at ambient temperature in 5% Goat sera in PBST before detection overnight (∼16 hours) at 4°C using an anti-Digoxigenin-Fab fragments conjugated to Alkaline Phosphatase enzymes (1:1000 dilution, Roche) in 5% Goat Sera in PBST ...
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bioRxiv - Genomics 2020Quote: ... before incubation at 37°C with 1 µg of double stranded DNA (atr amplicon, Jones et al., 2003) in the presence of 1x M buffer (Roche). Filtrated supernatants (10 µl ...
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bioRxiv - Immunology 2021Quote: Strand-specific ribosomal RNA depleted RNA-seq libraries were prepared from 1 µg of total RNA using the KAPA Stranded RNA-seq Kit with Ribo-Erase (Roche). Samples were prepared in at least triplicate for analysis ...
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bioRxiv - Microbiology 2021Quote: ... following which the pellet was re-suspended in 50 ml of buffer A (PBS with 10 mM DTT and 1 x protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were incubated with anti-digoxigenin conjugated with alkaline phosphatase (1:2500; sheep anti-DIG-AP Fab fragments, Roche Diagnostics ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the detection of the Dig-labelled riboprobe with an anti-DIG Fab fragment conjugated with alkaline phosphatase (1:750; Roche). The colometric reaction was performed using mixture of 5-Bromo-4-chloro-3-indolyl phosphate (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Transfected HEK29T cells and COS-7 cells were washed and lysed with 1% Triton X-100 in PBS with protease inhibitor cocktail (Roche) at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... cell lysates were retrieved in 1% sodium deoxycholate (SDC) in phosphate buffered saline solution with Protease Inhibitor Cocktail (cOmplete, Roche) buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Biochemistry 2020Quote: ... Membrane pellets were resuspended to 40 mg ml-1 in ice-cold buffer A supplemented with a protease inhibitor tablet (Roche) and 100 μM PMSF ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM Tris(2-carboxy-ethyl) phosphine (TCEP) containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... All qPCR reactions were performed in 10 μl volume in triplicates with 1× LightCycler 480 SYBR Green I Master kit (Roche) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) analyses of cDNA (diluted at 1/10) were performed in triplicate with a LightCycler 480 II (Roche) using the SYBR Green I Master mix (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were incubated live for 10 min at 37 °C with anti-HA rat monoclonal antibody (1:100, 11867423001, Roche) or anti-GFP mouse monoclonal antibody (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed using RIPA buffer (50 mMTris–HCl, 10 mM MgCl2, 20% Glycerol and 1% Triton X-100) containing protease and phosphatase inhibitors (Roche). Protein concentration was determined by BCA assay (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... Two days after induction, cells were lysed with lysis buffer (1x PBS, 1% NP-40, 1x protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with Glutamax and 10 % FBS were transfected using a 2:1 ratio X-treme GENE HP DNA transfection reagent (Roche) per μg of plasmid DNA with a total of 0.5 μg DNA per well ...
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bioRxiv - Cancer Biology 2021Quote: ... A 1/10 dilution of cDNA was used for qRT-PCR analysis using the FastStart Universal SYBR Green Master Mix (Roche). Sequences of the primers used are listed in the reagents or resource table.
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bioRxiv - Cell Biology 2021Quote: Cells were lysed with 0.1% Triton in PBS- (phosphate-buffered saline without CaCl2 or MgCl2) supplemented with EDTA-free protein inhibitor cocktail (complete, Roche) on ice ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were resuspended in ice-cold NP-40 Buffer (150mM NaCl, 1% NP-40, 50mM Tris pH8) containing EDTA free protease inhibitors (complete cocktail, Roche) and lysed using a 1ml tissue homogenizer (Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... and hybridized overnight at 55°C with 1 ng/ml digoxigenin-labelled (GGGGCC)5 oligonucleotide probe (Integrated DNA Technologies) in 25 ml hybridization reaction (EasyHyb Granules, Roche). Following denaturation of the probe at 95°C for 5 min ...
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bioRxiv - Plant Biology 2021Quote: ... and the presence of SnASFF1-HT was confirmed by immunoblot using the BMG–His-1 monoclonal antibody (Roche, Basel, Switzerland). Purified SnASFF1 proteins were stored in 100 mM sodium acetate (pH 4.5 ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed 2x with PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1X PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM sodium phosphate pH 8.0, 3% glycerol, 1% Triton X-100, 15 mM imidazole, and 1x protease inhibitor [Roche, Sigma]) and sonicated ...
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bioRxiv - Neuroscience 2021Quote: ... embryonic cortices were isolated and stored either in lysis buffer (48% Urea, 14 mM Tris pH 7,5, 8,7% Glycerol, 1% SDS, protease inhibitor cocktail (Roche, Basel, CH)) or RNAlater (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM KOAc, 2 mM MgCl2, 1 mM CaCl2, 0.2 M sorbitol, 10mM PMSF, 2x protease inhibitor cocktails [Roche, 11873580001]), dropped into liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... and digested in digest buffer (RPMI supplemented with 10% FBS, 1% penicillin/streptomycin [Invitrogen], 1mg/mL collagenase D [Sigma], 1mg/mL dispase [Sigma], 40 μg/mL DNAse [Roche]) at 37° C for 30 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was generated from 1 μg total RNA with qScript cDNA SuperMix (Quantabio) and analyzed on a LightCycler 480 II apparatus (Roche) with the SYBR Green I Master mix (Roche ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid NL4.3 and various mutants were transfected into JLTRG-R5 cells with 1 μg of viral DNA using Xtreme-GENE HP (Roche) Transfection reagent ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins were extracted in protein extraction buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 % glycerol, 2 mM EDTA, 5 mM DTT, 1 × EDTA-free Complete Protease Inhibitor Cocktail [Roche, USA] ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Genomics 2021Quote: ... Cells from two biological replicates were lysed with ChIP lysis buffer (5 mM PIPES pH8, 85 mM KCl, 1% Igepal) with a protease inhibitor (PI) cocktail (Roche). Nuclei were collected by centrifugation at 2,000 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... Lysis was performed using mechanical scraping in lysis buffer (1% IGEPAL-CA630 and 1mMPMSF with Roche complete protease inhibitor tablet) and subsequently spun down at 500xg for 5 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue samples were homogenized in pre-chilled lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, protease inhibitor cocktail (Roche 04693132001) and phosphatase inhibitor (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting powder was resuspended in 50 ml TAP Lysis buffer plus 1 mM PMSF and 1X Complete EDTA-free protease inhibitor cocktail (Roche). The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl ...
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bioRxiv - Microbiology 2021Quote: ... The column was washed with 200 mL of the equilibration buffer supplemented with 1 mM PMSF and EDTA-free protease inhibitor (Roche). SolPacL1 and SolPacL1Δ3 were then eluted with 25 mL of the equilibration buffer containing 1 mM PMSF and 250 mM imidazole.
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bioRxiv - Plant Biology 2021Quote: ... The construct was used to transform wild type plants following the standard floral dip method (Clough and Bent, 1998) and selected with 50 mg L-1 Hygromycin (Roche). A total of 60 T1 plants were individually genotyped by PCR and sequencing ...
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bioRxiv - Microbiology 2021Quote: Cells were washed once with ice-cold PBS and lysates harvested at indicated times post infection with lysis buffer (1% NP40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris HCl) supplemented with protease inhibitors (Roche – cOmplete mini EDTA-free protease inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... Cell toxicity at different concentrations of drugs as indicated was determine using the Cell Proliferation Reagent WST-1 (Roche Diagnostics) or cellular 96® Aqueous One Reagent viability reagent according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then lysed using 1x RIPA buffer (Thermo) supplemented with 3x HALT Protease inhibitors (Thermo) and 1 × PhosSTOP (Roche), 2 μl (500 Units ...
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bioRxiv - Cancer Biology 2020Quote: ... Transposed chromatin was amplified using customized Nextera PCR Primer 1 and Primer 2 (barcode) (31) and HotStart KAPA ReadyMix (Roche). Libraries were quantified by Qubit using the DS DNA HS kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... for 1h at RT and incubated with a primary antibody diluted in blocking solution (sheep anti-digoxigenin, 1/200, Roche) overnight at +4°C in a dark humidified chamber ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4, 200 mM NaCl, 1% NP-40, 2 mM MgCl2, 10% glycerol, NaF +Na3VO4, complete mini tablets without EDTA, Roche). Lysates were incubated on ice for 15 minutes and centrifuged at 17,000xg at 4°C for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated overnight at 4 °C in digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1:100; Roche), rinsed in TBS (0.1 M Tris-HCl with 0.9% NaCl ...