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Citations for Roche :
5151 - 5200 of 6180 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The adult inner ears were first were decalcified with 120 mM EDTA for 2 days at 4°C until they were soft and ready for micro-dissecting out the cochlear sensory epithelium for use in whole-amount preparation and immunostaining with the following first antibodies: anti-HA (rat, 1:200, 11867423001, Roche), anti-Prestin (goat ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA-probe hybrids were detected by an alkaline phosphatase-conjugated antibody (Anti-Digoxigenin-AP or Anti-Fluorescein-AP, Fab Fragments, 1:5000, Roche, Cat# 11093274910 ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 4 replicates of BCBL-1 WT clone B4 and 57KO clone #6 cells were isolated by TriPure Reagent (Roche) 24 h after induction with VA ...
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bioRxiv - Plant Biology 2022Quote: ... The ELVd probe was obtained via in vitro transcription of a linearized plasmid for 1 h at 37°C with 20 U of T3 bacteriophage RNA polymerase (Roche) in 40 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed on ice for 15 minutes with 100 µl lysis buffer (50 mM HEPES, 150 mM NaCl, 1% TritonX100) supplemented with protease and phosphatase inhibitor cocktail (Roche). 10% of the soluble fraction of the lysate was boiled with 4X sample buffer ...
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bioRxiv - Molecular Biology 2019Quote: Pellets from 2 ml cultures were resuspended in 500 μl SDS buffer (1% SDS, 10 mM EDTA, 5M Tris HCl, cOmplete Tablets Mini EDTA-free EASYpack (Roche), PhosSTOP (Roche)) ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: Immunological detection was performed using antidigoxygenin-AP Fab antibody fragments at a dilution of 1:2000 (Roche Diagnostics, Mannheim, Germany). After probe hybridization ...
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bioRxiv - Molecular Biology 2020Quote: ... 4×15cm plates of confluent WT F1 2-1 MS2129 female ESCs were lysed by pipetting in 3mL of lysis buffer (10M Tris-HCl pH8, 137mM NaCl, 1% NP40, 2mM EDTA) supplemented with 1x Complete EDTA-free Protease Inhibitors (Roche) and incubated for 1h on ice with or without RNase (10μg/mL RNase A ...
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bioRxiv - Molecular Biology 2021Quote: T98G cell pellets were fixed by dropwise addition of cold 70% ethanol and their DNA was stained with 4’,6-diamino-2-phenylindole (1 μg/ml, Roche), 0.1% Triton X-100 in phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Triton X-100, pH 7.4, containing phosphatase inhibitors (10 mM NaF, 1 mM Na3VO4) and protease inhibitors (cOmplete®, Roche) and the protein concentration was adjusted to 2 mg/ml using the Bradford method (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and were homogenised in TBS pH 7.4, 1% NP-40 containing phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche). Protein concentration was adjusted to 10 mg/ml by the Bradford method (Pierce ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequencing libraries were constructed from 1 ng of immunoprecipitated and input DNA using the KAPA Hyper Prep kit (KAPA Biosystems) and NEXTflex ChIP-seq barcodes (Bio Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... and the soluble fraction was incubated for 1 h at 4°C with beads crosslinked to mouse anti-GFP antibody (Roche). Resulting Immunoprecipitates were washed three times with lysis buffer and protein eluted with RapiGest surfactant (Waters ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Molecular Biology 2019Quote: Pellets from 75 ml cultures were resuspended in 500 μl SDS buffer (1% SDS, 10 mM EDTA, 5M Tris HCl, cOmplete Tablets Mini EDTA-free EASYpack (Roche), PhosSTOP (Roche)) ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear pellets were lysed by sonication on ice in 10 mL lysis buffer (50 mM Tris pH 7.4, 0.5 M NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, 1x Roche complete protease inhibitor). After sonication ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitors (Roche). The lysate was clarified by centrifugation and the proteins in the supernatant were purified by gravity Ni-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sections were blocked for 1 h with 2% blocking reagent in MABT (Perkin-Elmer) and then incubated with anti-digoxigenin-POD (Roche), diluted 1:500 ...
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bioRxiv - Immunology 2022Quote: ... In short lungs were minced with scissors and incubated in a digestion cocktail containing 1 mg/mL collagenase A (Roche) and 30 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in 500 μl lysis buffer (10 mM Tris HCl, 10 mM NaCl, 0.3% NP40, 1× Roche Complete) and kept on ice ...
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bioRxiv - Immunology 2021Quote: ... Colon tissue samples devoid of intra-epithelial cells as well as lung and skin samples were enzymatically digested with 1 mg/ml Collagenase A from Clostridium histolyticum (Roche) and 1 unit/ml DNaseI (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... PrPSc levels were determined through digestion of 20 μg of COCS homogenates with 25 μg mL-1 of proteinase K (PK, Roche) at a final volume of 20 μL in PBS for 30 minutes at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sense and antisense digoxigenin-labeled RNA probes were prepared from a BglII-digest of the pMPX2-1 mouse Pax6 cDNA clone (25) using a DIG RNA labeling kit (Roche). Hybridization and stringent posthybridization wash steps were performed at 65°C.
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bioRxiv - Genomics 2020Quote: ... Real-time quantitative PCR reactions were set up in triplicate with 2 μL of cDNA (1:4 dilution) per reaction using the FastStart Universal SYBR Green Master mix (Rox) (Roche) and run on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated 2 hr at room temperature with a 1:5000 dilution of alkaline phosphatase-conjugated anti-DIG primary antibody in block solution (Roche, cat #11-082-736-103 ...
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bioRxiv - Cell Biology 2019Quote: ... were lysed in NP-40 lysis buffer (50mM TRIS, pH7.5, 120mM NaCl, 1% NP-40) supplemented with Complete® protease inhibitor (Roche) and phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Paw tissues were minced and digested in 1 mg/ml collagenase A / 2.4 U/ml dispase II (Roche Applied Sciences) in HEPES buffered saline (Sigma ...
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bioRxiv - Biochemistry 2020Quote: Cells were resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Triton X100, 0.3 mM TCEP, 1X Roche cOmplete tablet) and lysed by three cycles of rapid freeze-thaw ...
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bioRxiv - Neuroscience 2019Quote: ... After 48 h of transfection cells were scraped and lysed in lysis buffer (20mM HEPES, 150mM NaCl, 1mM EDTA, 1mM EGTA, 1% Triton-X100, cOmplete Protease Inhibitor Cocktail (Roche), Benzonase (EMD Millipore) ...
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bioRxiv - Physiology 2019Quote: ... Tissue slices were incubated with hybridization probes for 18 h at 55°C and then incubated with sheep anti-DIG and anti-FITC antibodies conjugated with peroxidase (POD; 1:250; Roche) for probe labeling using the InSituPro VSI robot (Intavis ...
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bioRxiv - Molecular Biology 2019Quote: Total proteins were extracted in RIPA buffer containing 1 mM sodium orthovanadate and Complete Protease Inhibitor Cocktail (Roche Applied Science) and quantified using the Pierce™ BCA protein assay kit (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... a final probe concentration of 0.1 ng/μL was used and the probe was detected using an alkaline phosphatase conjugated anti-digoxigenin antibody (1:1500; Roche) and NBT/BCIP (Roche ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates from 5.0 × 106 cells were prepared using SDS-based buffer (5 mM EDTA, 1% SDS) supplemented with Protease inhibitor cocktail (Roche) and PhosSTOP EASY (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid DNA from the in vitro methylation reactions were transfected with 3 µL (Il33) or 1 µL (SV40) X-tremeGENE 9 transfection reagent (Roche) diluted in 50 µL of Opti-MEM medium (Gibco) ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were then probed for 16 h at 4 °C with the following primary antibodies in 3% (w/v) skim milk in PBS: mouse anti-GFP (1:1000, Roche), rabbit anti-SBP1 (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48-72 h and resuspended in lysis buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 30 mM imidazole, 1 mM TCEP and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed by sonication ...
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bioRxiv - Immunology 2020Quote: ... single cell suspensions were prepared from the tumors after mechanical disruption and enzymatic digestion with 1 mg/mL Type IV Collagenase (Roche). Live tumor infiltrating CD8+ T cells (CD3+ CD8+ CD4− ...
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bioRxiv - Microbiology 2019Quote: ... and lysed on ice using a lysis buffer (PBS, 0.1% SDS, 1% Triton X-100, EDTA-free complete protease inhibitor (Roche Diagnostics)) ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were then washed twice with cold PBS and resuspended using a cell scraper into lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1 % NaDOC, PMSF and Roche Protease Cocktail). Cells were sonicated and lysates were centrifuged at 16,000xg for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... allowed to adhere overnight and transfected with the indicated splicing reporter constructs (1 µg/well) using X-tremeGENE 9 (Roche). As shown in the Key Resources Table ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, 1 mM TCEP, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested 48h after transfection and lysed in lysis buffer (50mM Tris-Cl pH 8, 150mM NaCl, 1% Triton, 1x Roche Complete Mini Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in lysis buffer (1xPBS, 350 mM NaCl, 1% Triton X-100, 10% glycerol, EDTA-free Protease Inhibitor Cocktail (Roche), 30 mM imidazole) ...
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bioRxiv - Neuroscience 2019Quote: ... using 30:1 molar ratio of isotope [35S] dATP (1250Ci/mmol, NEN) to oligonoucleotide and terminal deoxynucleotidyl transferase (TDT, Roche). Non incorporated nucleotide was removed by Biospin 6 chromatography columns (Bio-rad) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antiviral azaBINOL B#24 was assessed for inhibition of HIV-1 reverse transcriptase using a commercially available colorimetric reverse transcriptase assay kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... and re-suspended in complete media (DMEM with 10% FBS and 1% antibiotics) supplemented with Collagenase-D (1mg/mL; Roche) and incubated at 37°C for 30 minutes with shaking to form a single-cell suspension ...
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bioRxiv - Synthetic Biology 2019Quote: ... Lymphocytes were isolated from tumor tissue by mechanical homogenization of tumor tissue followed and digestion with collagenase A (1 mg/ml; Roche) and DNase I (0.5 μg/ml ...
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bioRxiv - Microbiology 2019Quote: ... The cells were washed twice with PBS and lysed with 6 ml of lysis buffer (8 M urea, 150 mM NaCl, 100 mM ammonium bicarbonate, pH 8; added per 10 ml of buffer: 1 tablet of Roche mini-complete protease inhibitor EDTA free and 1 tablet of Roche PhosSTOP tablet ...