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Citations for Roche :
5051 - 5100 of 8095 citations for 7 Bromoacetyl 6 ethyl 1 2 3 4 tetrahydro 1 1 4 4 tetramethylnaphthalene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Fixed embryos were placed into hybridization buffer (50% formamide, 5×SSC, 1 mg/ml yeast tRNA (Roche, 10109223001), 100 μg/ml heparin (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Whole cell extracts were prepared in HEPES whole cell lysis buffer (20 mM HEPES, pH 7.4, 1% TX-100,1x phosphoSTOP complete inhibition cocktail tablets [Roche] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the samples were washed in 0.1M glycine pH 2.0 and then incubated overnight with anti-fluorescein (1:250) (Roche). Samples were then washed in TNT ...
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bioRxiv - Neuroscience 2021Quote: ... the reaction product was developed using NBT/BCIP solution diluted in pre-staining buffer (1:100; Roche, Switzerland) overnight at RT ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with an alkaline phosphatase-conjugated anti-digoxigenin antibody (1:1500 in blocking solution; Roche, Switzerland) overnight at RT ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM EDTA) supplemented with protease inhibitors (complete mini EDTA-free, Roche, 1 tablet / 50 mL lysis buffer). The lysed neurons were mixed with 4X Laemmli sample buffer and heated at 70°C for 10 minutes (stored at −20°C until use) ...
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bioRxiv - Neuroscience 2020Quote: ... The incubation with the anti-digoxigenin-POD took place afterwards (1/2000 dilution in BSA/TBST, Roche Diagnostics) at room temperature for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... 5 mM MgCl2, 20 mM Tricine-KOH, 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, 1X Roche EDTA-free protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2019Quote: ... First the tissue was mechanically dissociated using a tissue grinder while submerged in 1 ml of TriPure (Roche). 200 µl of chloroform (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... For CHIP-qPCR: qPCR was performed on diluted samples using LightCycler 480 SYBR green 1 master kit (Roche) using primers listed below.
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bioRxiv - Biochemistry 2020Quote: ... the medium was replaced by serum free DMEM supplemented with 1% fatty acid free BSA (Roche Applied Sciences) and a mixture of oleate and palmitate (ratio 2:1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in 450 μl lysis buffer (20 mM Tris pH 7.5, 0.5 mM EDTA pH 8, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 10 mM NEM, Roche cOmplete protease inhibitor catalog # 11836153001) ...
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bioRxiv - Plant Biology 2020Quote: ... 0.5% (w/v) PVP40 and Roche protease inhibitor cocktail (1 tablet per 50 ml of extraction buffer, Roche). After rocking on ice for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... 2 * 107 cells were pelleted and resuspended in 1 ml of lysis buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) including 0.25x protease inhibitor (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 5mM MgCl2, 0.5% NP-40, pH 7.4, supplemented with Complete Protease Inhibitor Cocktail, Roche). After centrifugation at 5000g for 10minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 350 μl of lysis buffer (20 mM Tris, pH 7.5, 0.5 M NaCl, 1 mM EDTA, 1x protease inhibitor cocktail [Complete; Roche]) and 200 μl of glass beads (425-600 μm ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked with 5% non-fat milk TBST and probed with primary anti-GFP (Roche, 1:1000) and secondary HRP- conjugated anti-mouse antibody (R&D Systems ...
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bioRxiv - Molecular Biology 2020Quote: Adherent cells were washed twice with PBS and then scraped and digested on ice for 30 min with a homemade RIPA (50 mM Tris-HCl pH 7.5, 15 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS, supplemented with Protease Inhibitors [Roche] and Phosphatase Inhibitors [Sigma-Aldrich]) ...
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bioRxiv - Microbiology 2019Quote: ... pH 7.4, 500 mM NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, and 1x complete protease inhibitor [Roche]) and sonicated ...
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bioRxiv - Microbiology 2020Quote: ... permeabilized and immunostained with the following primary antibodies - rat monoclonal anti-HA (clone 3F10, Roche: 1:250 dilution), mouse monoclonal anti-myc (clone 9B11 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μl 1xTaqMan Gene Expression Assay primer and 10 μl 1x FastStart Universal Probe Master-mix (Roche, 04913957001) were applied ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with primary antibodies used at the following dilutions: mouse-anti-HA (Roche, 1:300), rabbit-anti-γ-H2A.X (Cell signaling ...
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An optimized ChIP-Seq framework for profiling of histone modifications in Chromochloris zofingiensisbioRxiv - Genomics 2021Quote: ... The cell pellet was resuspended in 1 mL ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-Cl, pH 8.0, and 0.25 x protease inhibitor cocktail [Roche]) and transferred to Beckman Coulter 4mL Polycarbonate Thick Wall Centrifuge Tubes (13 × 64 mm) ...
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An optimized ChIP-Seq framework for profiling of histone modifications in Chromochloris zofingiensisbioRxiv - Genomics 2021Quote: ... Cell pellet was resuspended in 1 mL ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-Cl, pH 8.0, and 0.25 x protease inhibitor cocktail [Roche]) and transferred to Beckman Coulter 4mL Polycarbonate Thick Wall Centrifuge Tubes (13 × 64 mm) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.3% (v/v) Tween-20) for 5 min under agitation and next blocked in 1× blocking buffer (Roche) for 30 min at room temperature with gentle shaking ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated in PEMBALG containing monoclonal anti-HA antibody produced in rat (1:1000 dilution, Roche) for one hour ...
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bioRxiv - Biochemistry 2022Quote: ... and 10% glycerol] containing the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche). The lysate was frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Genomics 2022Quote: ... twice 10 min with cold TET buffer (10 mM Tris/HCl pH7.5, 1 mM EDTA, 0.1% Tween-20, protease inhibitor cocktail (Roche)) and eluted with TE buffer ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with 40 µL of primary antibody (1:1,000 rat anti-HA mAb 3F10, Roche) at 4°C overnight in a solution containing 3% BSA in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Embryos were transferred to 24 well plates and NTMT-NCP-BCIP mix (1:200 dilution NCP-BCIP:NTMT (Roche)) was added ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were blocked with 5% milk dissolved in TBST and probed with primary anti-HA (Roche, 1:2000), anti-Flag (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... The dermal sections were digested for 1 hour at 37°C in 0.5 mg/ml Liberase TM (Roche) in serum free RPMI (Gibco + 2mM glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... pH 7.4 with 1% Triton X100 and 1x Complete Mini Protease Inhibitor Mixture (Roche Applied Science, Indianapolis, IN). Cell lysate was cleared by centrifugation at 15,000x g for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... 1.5 mM magnesium acetate] containing 5 mM dithiothreitol (DTT) and 1 × EDTA-free Complete Protease Inhibitor tablets (Roche) by tapping and inverting the tubes ...
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bioRxiv - Microbiology 2020Quote: Whole-cell extracts were obtained from liver tissue or cell pellets lysed in buffer (0.1%, sodium dodecyl sulfate, 0.1% sodium deoxycholate, 1% IGEPAL) supplemented with Protease and Phosphatase Inhibitor cocktails (Roche). Protein extracts were separated by SDS-polyacrylamide gel electrophoresis then transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked using 10% skimmed milk in PBS containing 0.1% Tween 20 (PBST) and then incubated with rat anti-HA high affinity (1:1,000; Roche) and rabbit anti-T ...
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bioRxiv - Microbiology 2019Quote: ... shaved skin was incubated in I10F medium in the presence of 1 U/ml of dispase (Roche Diagnostics) for 1 h at 37° C or 0.5 U/ml overnight at 4° C ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was aspirated and the pellet was resuspended in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS supplemented with Roche protease and phosphatase inhibitor tablet just before use) ...
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bioRxiv - Neuroscience 2020Quote: ... 1 M NaCl and protease inhibitor cocktail (cOmplete, Mini, Roche; two tablets per 10 mL of lysis buffer). The lysate was sonicated before AHA-labeled proteins were covalently coupled to alkyne-tagged agarose beads using reagents provided in the kit ...
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bioRxiv - Genomics 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Tris, pH 7.5, 1 mM EDTA, 0.5% Nonidet P-40, protease and phosphatase inhibitor cocktail [Roche]). Cell lysates were denatured in Laemmli sample buffer and incubated for 5 min at 95°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and washed three times with 1 mL digitonin buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Roche complete protease inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... Fcgr1 mRNA signal was then detected using sheep anti-DIG antibody (1:500; Roche Diagnostics Corp., Indianapolis, IN) followed by the secondary antibody (Donkey anti-sheep IgG Alexa 488 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS)39 ...
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bioRxiv - Immunology 2020Quote: ... The eluate was then treated with RNaseA for 1 h at 37°C and with Proteinase K (Roche) for 1 h at 55°C and the DNA was recovered using a Qiagen PCR purification kit.
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bioRxiv - Microbiology 2021Quote: ... The supernatant was removed and the pellet was dissolved in 500 µl of ice-cold nuclear solubilization buffer (25 mM HEPES [pH 6.8], 1 M sorbitol, 0.5% NP-40, 250 μg/ml PMSF, cOmplete [EDTA-free protease inhibitor cocktail; Roche]). Following incubation for 30 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed in lysis buffer (1.5M NaCl, 20 mM Phosphate buffer pH 7.5, 20 mM Imidazole, 10mg/mL lysozyme, 1 tablet of Roche EDTA-free protease inhibitor cocktail Millipore Sigma 11873580001 ...
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bioRxiv - Microbiology 2021Quote: ... and 0.1 mM CaCl2) with or without DNase I (final concentration 160 U mL-1, Roche, cat. # 04716728001) and were incubated at 37°C for 3 h ...
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bioRxiv - Genomics 2022Quote: ... The ConA bound nuclei were then incubated with primary antibody diluted 1:100 in Antibody Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2022Quote: ... For enteric glial cell experiments were performed by hypotonic lysis in 200 µl of buffer A (20 mM HEPES, pH 8.0, 1 mM DTT, 0.1% NP-40 and a proteinase inhibitor cocktail from Roche). The cell lysates were incubated on ice for 10 minutes ...