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Citations for Roche :
5001 - 5050 of 8095 citations for 7 Bromoacetyl 6 ethyl 1 2 3 4 tetrahydro 1 1 4 4 tetramethylnaphthalene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... EBs were dissociated into single cells by treatment with 1 mg/ml Dispase II (Roche, 04942 078001) and 2.5 mg/ml collagenase I (Gibco ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were transfected with Fugene 6 (Roche). The day before transfection 5 x 105 N2A cells were plated on 6 cm plates ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 U/ml glucose-6-phosphate dehydrogenase (Roche), 2.5 U/ml hexokinase (Roche) ...
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bioRxiv - Biochemistry 2022Quote: ... Glucose-6-phosphate (G6P) (#10127647001) is from Roche. All other chemicals if not noted otherwise are from Sigma Aldrich.
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bioRxiv - Systems Biology 2022Quote: ... 6 cycles of preamplification with KAPA HiFi (Roche), and purification with Ampure XP beads (1:1 ratio ...
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bioRxiv - Developmental Biology 2022Quote: ... using FuGENE 6 transfection reagents (Roche, Basel, Switzerland). The cells were selected with puromycin (1 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Transient transfections were performed using FuGene 6 (Roche) for HEK293 FT cells and Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Systems Biology 2019Quote: ... 6 µl X-treme Gene transfection reagent (Roche), and 100 µl Opti-MEM media (Life Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... 6 U/ml Pyruvate kinase (Roche, ref: 10128155001), 9 U/ml L-lactate dehydrogenase (Roche ...
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bioRxiv - Immunology 2023Quote: 7-plex IF panel was created using the Ventana BenchMark Ultra (Roche Diagnostics) automated staining platform ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
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bioRxiv - Developmental Biology 2021Quote: INR was measured in a drop of fresh whole blood (6 Jag1+/+ and 6 Jag1Ndr/Ndr mice at P10) with a commercially available point of care coagumeter (CoaguChek® XS, Roche).
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transfected with HSIV-vif plasmids using Fugene 6 or X-tremeGENE 9 DNA transfection reagent (Roche/Sigma). At 48 hour post-transfection ...
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bioRxiv - Microbiology 2020Quote: IL-6 release over time was studied in the Laboratoriumsmedizin of the Munich University using an ELISA (Roche, Elecsys IL-6).
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bioRxiv - Cancer Biology 2020Quote: ... All the signals were visualized by adding 3-3′-Diaaminobenzidinetetrahydrochloride (DAB substrate) solution (Roche) to the slides and counterstained with haematoxylin ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.65% Nonidet P-40 and 1 mM phenylmethylsulfonyl fluoride) supplemented with proteinase inhibitor mixture (cOmpleteTM EDTA-free; Roche). The nuclei were harvested by scraping into a 1.5-ml microcentrifuge tube and collected by centrifugation at 500 × g for 5 min at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... the membrane was first probed with an anti-GFP antibody (Cat.No. 11814460001, Roche, Basel, Switzerland; 1:3’000 dilution) followed by a secondary anti-mouse-IR800 (R-05061 ...
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bioRxiv - Cell Biology 2020Quote: Cell suspension (1 million cells/ml) was mixed with 6x SDS loading buffer and 10 mM DTT (Roche) and incubated at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with the anti-HA high affinity antibody (3F10) (1:500 dilution, Roche – cat. 11867423001), followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... harvested cells were incubated with 25 μg/ml digitonin in buffer H (20 mM Hepes-KOH, pH 7.4, 0.25 M sucrose, 1 mM DTT, complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated at room-temperature with a 1:500 dilution of anti-Dioxigenin-AP Fab fragments (Roche, #11093274910) in 1% BSA/0.3 % Triton-X100/TBS for 1hour ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from asynchronous RPE-1 cells using the High Pure RNA Isolation kit (Roche, 11828665001) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed in ice cold RIPA buffer (50mM Tris HCL, 150mM NaCl, 0.1% SDS, 0.5% DOC, 1%NP-40, EDTA-free protease inhibitor (Roche), phosphatase inhibitors (Halt phosphatase ...
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bioRxiv - Genetics 2021Quote: ... livers were resuspended in a total volume of 4.687 ml of XL Buffer (50 mM Hepes, pH 7.5, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1X Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Genetics 2021Quote: ... Harvested cells in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated in alkaline phosphatase-conjugated anti-DIG (Digoxygenin) antibody Fab fragments (1:5000; Roche, catalog #12486523) at 1:5000 in the blocking buffer and incubated at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... washed with 500 μL buffer MB#1 (10 mM Tris-HCl, pH 7.5, 50 nM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche complete EDTA-free (Roche diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed with a LightCycler® 480 SYBR Green 1 Master reaction mix (Roche Diagnostics, Mannheim, Germany), following the manufacturer’s temperature program with 60°C for the primer hybridization and elongation temperature.
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EDTA, 300 mM NaCl, 10 mM Tris.HCl [pH 8.0], 1 mM EDTA, 1x Complete protease inhibitor [Roche] ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM MgCl2, 10 % v/v glycerol, 140 mM NaCl, 0.1 mM EDTA, 0.5 % Tween20, 1 mM DTT and Roche cOmplete-Ultra EDTA free protease inhibitor) ...
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bioRxiv - Molecular Biology 2019Quote: ... with addition of 1 mM phenylmethylsulfonyl fluoride (PMSF) and cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche). His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) and sonicated for 90 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% NP40 and 10 mM Tris (pH 7.5)) supplemented with cOmplete EDTA-free protease inhibitor (Roche, Basel, Switzerland). Samples were incubated on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 350 μl of lysis buffer (20 mM Tris pH 7.5, 0.5 M o NaCl, 1 mM EDTA, 1X protease inhibitor cocktail [Complete; Roche]) and 200 μl of glass beads (425-600 μm ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed three times on the magnet in 1 mL buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Roche complete protease inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Nonidet-P40, 0.2 mM TCEP, and Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Immunology 2019Quote: ... 50 mM KCl and 0.45% Tergitol at pH 8.0) that was freshly supplemented with 1 μg/mL proteinase K (Roche). After deactivating proteinase K by incubating for 15min at 95 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... Supernatant containing cytosol protein was mixed with 1% SDS solution supplemented with proteinase inhibitor (PI) cocktail (Roche, 5892970001), and then was used for western blotting ...
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bioRxiv - Immunology 2019Quote: ... 1% Pen/Strep) containing 0.25 mg/mL Liberase TL and 20 U/mL DNase I (both from Roche), stirring at 37°C for 1h ...
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bioRxiv - Physiology 2020Quote: ... The sections were incubated with sheep anti-DIG antibody (1:200, Roche Applied Science; cat. no 1333 089), biotinylated donkey anti-sheep antibody (1:200 ...
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bioRxiv - Genetics 2019Quote: ... with cDNA then being generated from 1 μg of RNA utilizing the First Strand Reverse Transcriptase kit (Roche). Quantitative PCR was utilized to assess target gene expression using the KAPA SYBR Fast QPCR Master Mix ...
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bioRxiv - Molecular Biology 2019Quote: ... resuspended in modified RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Igepal, 0,25% Na-Deoxycholate, protease inhibitor cocktail without EDTA-Roche), homogenized through a 23G needle on ice ...
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bioRxiv - Evolutionary Biology 2019Quote: ... HIV-1 genomic RNA was extracted from supernatants containing virions using High Pure Viral RNA kit (Roche Inc.). SuperScript III and a primer downstream of the randomized regions were used to reverse transcribe viral RNA to cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... cardiomyocytes were dissociated using DPBS for 20 min at 37 °C followed by 1:200 Liberase TH (Roche) diluted in DPBS for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2019Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10,000; Roche Diagnostics, Germany). The color staining was carried out with chromogen substrates (nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate ...
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bioRxiv - Immunology 2019Quote: ... and each LN was placed in 1 mL of pre-warmed digestion media (0.8 mg/mL dispase, 0.2 mg/mL collagenase P (Roche), 50 µg/mL of DNase I in LN media) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or p89.6) and an envelope deficient HIV-1 backbone vector (pSG3Δenv) using XtremeGENE HP DNA Transfection Reagent (Roche). After 24 hours of incubation at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2019Quote: ... then immersed in 0.1 M maleate buffer (pH 7.5) for 10 minutes before blocking in 1% Blocking Reagent (Roche) for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1% SDS) and subsequently resuspended in 900 µL shearing buffer with added 1× protease inhibitors complete mini (Roche). Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode ...
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bioRxiv - Genomics 2021Quote: ... Cells were lysed by the addition of 1000 μL lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8.0, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice to generate an average DNA fragment size of ∼250 bp ...