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Citations for Roche :
451 - 500 of 699 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Blotting was done as described in (Konrad et al. 2010) using primary rat-derived anti-HA antibody (dilution 1:500, Roche) followed by anti-rat (dilution 1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with primary antibody for rat anti-HA (1:200; 12013819001, RRID:AB_390917, Roche, Basel, Switzerland), rabbit anti-GFP (1:200 ...
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bioRxiv - Microbiology 2019Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with antibodies against rat anti-HA clone 3F10 (Roche) or rabbit anti-TgSKP1 UOK75 [19]
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bioRxiv - Biochemistry 2019Quote: ... The antibodies used for the brain-ring gland complex included a rat anti-HA high-affinity monoclonal antibody (3F10, 1:20 dilution; Roche), a guinea pig anti-Shroud antibody (67 ...
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bioRxiv - Cell Biology 2021Quote: ... Retinas from adult Long Evans rats were dissected in DMEM/F12 media supplemented with complete protease inhibitors (Roche, Indianapolis, IN) and positioned above a disc of nitrocellulose disc oriented photoreceptor-side down ...
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bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... HA-tagged pUL97 kinase was immuno-precipitated from transfected HEK 293T cells using the rat anti-HA antibody clone 3F10 (Roche). Kinase inhibitors were added to the kinase reactions at final concentrations of 1 μM (CDKIs ...
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bioRxiv - Bioengineering 2023Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
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bioRxiv - Bioengineering 2024Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were permeabilised by 10 minutes incubation with 0.1% Triton X-100 on ice and incubated with blocking buffer (10% FBS in 1X PBS) for 90 minutes and then probed with the primary antibody (anti-HA rat 1:100 (Roche) for 2 hours on rotor and after three washing with 1x PBS cells were probed with anti-rat Alex Flour-488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Microbiology 2024Quote: ... BSA in TBST (TBSTB) and probed with rat monoclonal Anti-HAHA High Affinity antibody (clone 3F10, cat. no. 11867423001, Roche) diluted 1:1000 in TBSTB for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... pseudosite attD plasmids were PCR amplified using the KAPA Hifi HotStart ReadyMix (Roche), amplifying the pEF-1α promoter followed by mCherry ...
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bioRxiv - Immunology 2022Quote: ... and 0.8 μg of transfer plasmid using 5.8 μl X-tremeGENE HP (Roche). Media was replaced after 16 hours and supernatant harvested after a further 24 hours by filtering through a 0.45 μm cellulose acetate filter ...
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bioRxiv - Cell Biology 2020Quote: ... coli liquid cultures using the High Pure plasmid isolation kit (Roche, Basel, Switzerland).
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bioRxiv - Cancer Biology 2020Quote: ... The DF1s were transfected with various RCAS plasmids using X-TremeGENE 9 (Roche). Primary brainstem gliomagenesis was induced by injecting NestinTVA mice (postnatal days 3-5 ...
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bioRxiv - Molecular Biology 2021Quote: Cells were transfected with indicated plasmids using Fu-Gene6 transfection reagents (Roche Diagnostics), Lipofectamine 2000 transfection reagents (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ligated to gel-purified plasmid backbones using T4 DNA ligase (Roche; 10799009001). New England Biolabs high efficiency competent 5α E ...
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bioRxiv - Microbiology 2023Quote: ... We recircularized linearized plasmid PCR products with a Rapid DNA Ligation Kit (Roche) and transformed plasmids into DH5α-competent cells (Thermo Fisher) ...
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bioRxiv - Biochemistry 2022Quote: ... Transient plasmids transfections were performed using X-tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer’s instructions next day ...
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bioRxiv - Cell Biology 2023Quote: Plasmids containing a sgRNA were transfected into HeLa cells with X-tremeGENE8 (Roche). Single GFP-positive cells were sorted by fluorescence-activated cell sorting (FACS ...
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bioRxiv - Microbiology 2019Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
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bioRxiv - Genomics 2019Quote: TUNEL staining on rat β-cells was performed 48h after transfection using the TMR red In Situ Cell Death Detection Kit (Roche) combined to polyclonal guinea pig anti-insulin (dilution 1:40 ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were re-probed to detect ZMPSTE24 and Rce1 using rat anti-HA (clone 3F10, Roche cat #11867423001; 1:10000 dilution), and polyclonal rabbit anti-Rce1 [52] (1:2000 dilution) ...
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bioRxiv - Neuroscience 2020Quote: ... Rat pups were injected with BrdU (i.p. 50 mg kg-1 day-1 BrdU in saline; Roche Diagnostics, Indianapolis, IN, USA) for 5 consecutive days ...
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bioRxiv - Plant Biology 2021Quote: ... 150mM NaCl] supplemented with 1% Tween® 20 for 30 min before probing the membrane with either rat monoclonal anti-HA antibody (3F10, Roche) or mouse monoclonal ANTI-FLAG® antibody conjugated to HRP (M2 ...
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bioRxiv - Plant Biology 2020Quote: ... transferred to a PVDF membrane and immunoblotted with rat monoclonal anti-HA (High Affinity, clone 3F10, Roche, www.roche.com; 1:2000 dilution) and hybridized with peroxidase conjugated goat anti-rat (Polyclonal ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by rabbit anti-Rat (AI-4001, Vector) at 1:500 for 32 min and OmniMap™ anti-Rb HRP (760-4311, Roche). Blocking was performed using casein (760-219 ...
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bioRxiv - Neuroscience 2021Quote: ... the L4/5 DRG were rapidly harvested from the isoflurane anesthetized naïve rats and were incubated in 0.01% blendzyme 2 (Roche Diagnostics, Madison, WI) for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... falciparum ACT1 (final dilution 1:500; a kind gift from Jake Baum) or rat anti-HA (1:1000; Roche, Basel, Switzerland) for HA-tagged GAP45 and AMA1 ...
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bioRxiv - Plant Biology 2023Quote: ... Primary and secondary antibody dilutions used for immune detection were as follows: α-HA(rat)-1:2,000 (Roche Diagnostics, Basel, Switzerland), α-GFP(mouse)-1:1500 (Roche Diagnostics ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extract preparation and immunoblotting of Chlamydomonas cultures were performed as described previously (46, 48) with the following modifications: Antibody dilutions were: rat-anti-HA (1:5000) (3F10, Roche, Switzerland), Anti FLAG rabbit polyclonal (Rockland 600-401-383 ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used for the preparation of stained gels for expansion microscopy: Rat anti-HA (3F10, 1:25, Roche 12158167001), Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ChIP followed by qPCR or Illumina sequencing was performed as described58 using the 3F10 rat anti-HA antibody (1:500; Roche no. 11867423001) to immunoprecipitate HA tagged AP2-HS ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then incubated for 1 h at 4° C in PBS + 1% BSA with a 1:1000 dilution of rat anti-HA monoclonal antibody (Roche; Basel, Switzerland) and washed 5×10 min on ice with PBS + 1% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections containing the medial prefrontal cortex (mPFC) or hippocampus were incubated with (1) an antibody cocktail of rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-PV (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... the membranes were incubated with primary antibody for 2.5 h (GFP, Abcam, Cambridge, UK: rabbit, 1:10000; HA [Roche], rat, 1:3000). Excess primary antibody was then removed by washing three times in 2x TBST (150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001; clone 3F10, monoclonal from Roche, 1:5000 in 5% milk) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was extracted from recombinant clones with the High Pure Isolation kit (Roche) as directed by the manufacturer and sequenced in an ABI PRISM 3100 genetic analyzer (Applied Biosystem ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid transfections were carried out using X-tremeGENE 9 DNA transfection reagent (Roche Diagnostics) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... directly from genomic DNA or cloned plasmid templates to incorporate DIG-labeled dUTP (Roche) using the primers described in Extended Data Table 5 ...
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bioRxiv - Microbiology 2019Quote: ... large-scale insertions and plasmid sequences were assembled de novo using Newbler (Roche, Inc.), and integrated into the genome where connectivity was supported by evidence from paired reads ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfections were performed after cells had reached ~90% confluency using Lipofectamine reagent (Roche), as recommended by the manufacturer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The plasmid constructs were transfected into HEK293 cells by using X-tremeGENE HP (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The plasmid transfections were carried out with X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were replated in 96-well plates the next day at 2 × 104 cells/well density and transfected with poly I:C (700 ng in 110 μl volume ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with plasmid DNA using X-tremeGENE HP DNA transfection reagent (Roche) as per the manufacturer’s protocol using a total vector amount of 2 µg (6-well ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were incubated for 20 min with a mix of XtremeGENE9 transfection reagent (Roche) and OptiMEM (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were transfected into HEK293T cells using X-tremeGENE 9 (Roche # XTG9-RO) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... We re-circularized linearized plasmid PCR products with a Rapid DNA Ligation Kit (Roche) and transformed plasmids into DH5α-competent cells (Thermo Fisher) ...