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Citations for Roche :
451 - 500 of 8282 citations for 6 OXO 1 2 DIHYDRO 6H PYRROLO 3 2 1 IJ QUINOLINE 5 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and blocked in 10% Sheep Serum for 2 hours followed by incubation with an anti-DIG antibody (1:2000) (Roche) in TBST / 1% sheep serum overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... the trachea was catheterized and BAL was performed by 2 consecutive flushes of the lungs with 1 mL ice-cold PBS containing Complete Protease Inhibitor Cocktail (Roche). Cell density in BAL fluid (BALF ...
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bioRxiv - Genomics 2020Quote: ... the tissues were cut into 1-2 mm2 pieces and put in 1mg/ml collagenase and dispase (C/D) solution (Roche Diagnostics GmbH Roche Applied Science ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed once with PBS and resuspended in ice-cold WCE buffer (10 mM Tris-HCl, 1% NP-40, 2 mM MgCl2, benzonase, cOmplete Protease Inhibitor Cocktail (Roche), 25 nM NEM where relevant ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice in cold 1× PBS and harvested in 2% SDS in 50 mM HEPES buffer with freshly added protease and phosphatase inhibitors (Roche). Cell lysates were sonicated and centrifuged for 15 min at 12000 ×g to remove the insoluble fraction ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were homogenized in lysis buffer (7 M urea, 2 M thiourea, 1% CHAPS, 70 mM DTT, and Roche EDTA-free complete protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.1% NP-40, 1 mM DTT, 10 mM NaF, and EDTA-free protease inhibitor cocktail, Roche, Germany), and centrifugation at 20,000 × g for 3 min ...
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bioRxiv - Cancer Biology 2022Quote: A total of 20-50 mg of snap-frozen melanoma tissues were pulverized by Geno/Grinder for 2 min at 1500 rpm and then fixed with 1% methanol-free formaldehyde plus protease inhibitor cocktails (Roche) for 10 min at room temperature and quenched by 125 μM glycine for 5 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated in an anti-digoxigenin antibody (alkaline-phosphatase-coupled anti-digoxigenin; RRID:AB_514497, diluted 1:3,500; Roche Diagnostics; Table 2) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Thawed cells were resuspended in solubilization buffer (20 mM HEPES pH 8, 500 mM NaCl, 2 mM MgCl2, 15% glycerol, 1 Roche EDTA free Protease inhibitor tablet ...
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bioRxiv - Neuroscience 2023Quote: Mouse brains at 5W were isolated and homogenized in homogenization buffer [(0.32 M sucrose, 10 mM HEPES, 2 mM EDTA and 1× complete protease inhibitor cocktail (Roche Diagnostics), pH 7.4)] ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 4°C overnight with the primary antibody mouse IgG1 Anti-Tbx6 (home-made, clone A83, dilution 1:500) in 2% Blocking Reagent (Roche) and 5% sheep serum (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... minced with scissors and razor blade in the presence of 1 ml of digest media (2 mg/ml collagenase IV (Roche), 1 mg/ml hyaluronidase (Worthington) ...
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bioRxiv - Microbiology 2024Quote: ... and lysed by incubation with nondenaturing lysis buffer (300 mM NaCl, 100 mM Tris-HCl [pH 7.4], 2% Triton X-100, and 1×EDTA-free protease inhibitor cocktail [Roche Complete]) for 30 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... colorimetric reaction was performed for 1-2 days at room temperature in a solution containing NBT (nitro-blue tetrazolium chloride, Roche) and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA and 1% Triton X-100) containing 2 mM Na3VO4 (Sodium Orthovanadate) and 1 mM pmsf (phenylmethylsulfonyl fluoride) and 1x protease inhibitor cocktail tablet (Roche). Whole cell lysates were centrifuged at 12,000 rpm at 4 0C for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: The V4 hypervariable region of the 16S rRNA gene was amplified with the primer pair 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3) on a Fast Start High Fidelity PCR System (Roche, PQ) using the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of pyrophosphatase at 5 μg/ml (Roche), 4 μl of 1 mg/mL T7 RNA polymerase and 20 μl of transcription buffer 5X (Hepes pH7.5 400 mM ...
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bioRxiv - Neuroscience 2022Quote: ... 5 mM KCl) with 1% protease inhibitor cocktail (Roche), 1% phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... 1% deoxycholate) and 5% protease inhibitor cocktail (Roche, Germany). Whole cell extracts were incubated at 4°C for 30 min on a shaker ...
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bioRxiv - Genomics 2023Quote: ... protease inhibitors (1 tablet per 5 mL, Roche 4693159001)) was added to the embryo pellet ...
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bioRxiv - Immunology 2023Quote: ... Following antibodies were diluted in 1-5% BSA (Roche): anti-cGAS (Cell Signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 mM type 1 collagen (Roche Diagnostics) to allow spheroid formation and seeded onto the ULA plates at a concentration of 5 × 103 cells per well ...
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bioRxiv - Developmental Biology 2019Quote: ... third-instar larvae were dissected in PBS and incubated with 5-bromo-2’-deoxy-uridine (10 μM, Roche) for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2 μl diluted cDNA (5 ng total RNA equivalents) were analyzed on the LightCycler 480 instrument (Roche) using two replicates ...
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bioRxiv - Cancer Biology 2023Quote: Proliferation was measured by using 5-bromo-2’-deoxy-uridine (BrdU) labelling and detection kit (Roche, BSL, CH) as described earlier (25) ...
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl, 5 mM Beta glycerophosphate, 0.1 mM sodium orthovanadate, 2 mM TCEP, 0.4% NP40, 1X Roche EDTA free mini complete protease inhibitor) ...
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bioRxiv - Immunology 2023Quote: ... HEK293T cells were lysed in LUMIER lysis buffer (50 mM Hepes-KOH pH 7.9, 150 mM NaCl, 2 mM EDTA, 0.5% Triton X-100, 5% glycerol and Roche cOmplete™ Mini protease Inhibitor Cocktail ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 μL of each sample was used for qPCR with 2 × Sybr Green (Roche) and primers for human RPLP2 (housekeeping gene) ...
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bioRxiv - Microbiology 2022Quote: ... (2) the quantitative Roche Spike Elecsys Anti-SARS-Cov-2 S assay (Roche, IND, USA), which measures spike total antibody concentrations ...
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bioRxiv - Biochemistry 2021Quote: ... 137.5 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1 mM sodium fluoride, EDTA-free protease inhibitor cocktail [Roche]). 1 mM orthovanadate was added to the lysis buffer to prevent binding of substrates to the PTP1B trapping mutant ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was measured 6 days post transfection using WST-1 reagent (Roche). Cells were incubated with 10 μl of WST-1 per 100 μl of media for 1 hour and absorbance was read at 450 and 630 nm ...
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bioRxiv - Genomics 2023Quote: ... 6 μL of Ligation-1 mix (3.75 U T4 DNA ligase (Roche, 10799009001), 33.3 mM DTT (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Pools of 6 ganglia were homogenized in 1 ml TriPure isolation reagent (Roche) using lysing matrix D on a FastPrep24 instrument (3 cycles of 40 seconds at 6 m/s) ...
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bioRxiv - Microbiology 2023Quote: ... Pools of 6 ganglia were homogenized in 1 ml TriPure isolation reagent (Roche) using lysing matrix D on a FastPrep24 instrument (3 cycles of 40 seconds at 6 m/s) ...
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bioRxiv - Cell Biology 2020Quote: ... Cell viability was assessed by addition of 5 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (i.e., MTT) using a Cell Proliferation Kit I (Roche Diagnostics, Mannheim, Germany), in accordance with the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... 10 μL / well of MTT (3- [4,5-dimethylthiazol-2-yl] −0,5-diphenyl tetrazolium bromide (11465007001; Roche Life Science, Mannheim, Germany) labeling reagent (1× ...
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bioRxiv - Molecular Biology 2019Quote: ... and viability was measured using the Roche MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) kit (Roche, Cat # 11465007001) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Metabolic activity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-assay (Cell Proliferation Kit I, Roche Germany, Mannheim) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The beads were then collected at 1000 g for 2 min and washed at least 3 times with lysis buffer coupled with protease inhibitor cocktail (Roche). After last wash and centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... uteri were harvested from pregnant mice at 4.5 days post coitus and washed with cold swelling buffer (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 1X Protease Inhibitor Cocktail (PIC, Roche, 11836170001)) immediately after collection ...
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bioRxiv - Cell Biology 2022Quote: ... DNA (1 μg) was mixed with 3 μl Fugene6 (Roche, #11836145001) in 200 μl opti-MEM (Gibco ...
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bioRxiv - Immunology 2019Quote: ... After blocking for 1 hour in 3% Bovine Serum Albumin (Roche) in TBS-T ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche] in Maleic acid) for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Developmental Biology 2023Quote: ... 5-Bromo-4-chloro- 3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...