Labshake search
Citations for Merck :
151 - 200 of 223 citations for pVectOZ GFP Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... Viral supernatant was harvested at 48 and 72 h post-transfection and filtered through a 0.45 μm PVDF membrane (Merck Millipore). For lentiviral infection ...
-
bioRxiv - Biochemistry 2021Quote: ... Transient transfection of U2OS and HepG2 cells with plasmid DNA was carried out using GeneJuice (Merck Millipore, cat. number 70967) according to manufacturer’s instruction and keeping GeneJuice to DNA ratio of 3:1 (volume in μl ...
-
bioRxiv - Immunology 2022Quote: ... were co-electroporated in BLaER1 cells using a Biorad GenePulser device or co-transfected in 293T cells in combination with GeneJuice transfection reagent (Merck). 24 h later ...
-
bioRxiv - Genetics 2022Quote: ... Transient transfection was carried out in HEK293T cells transfected with 1 μg of plasmid DNA in the presence of X-tremeGene9 DNA transfection reagent (#6365809001, Merck), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Titers were determined by transduction of Jurkat cells (ACC 282; DSMZ) in presence of 5 µg/ml Polybrene Infection/Transfection Reagent (Merck). After 48 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... At 48 hours of transfection (If indicated) the cells were treated with the following conditions before harvesting at indicated times: 2mM of H2O2 (MERCK) for 1 hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... Conditioned medium containing recombinant lentivirus was collected 48hrs after transfection and filtered through non-pyrogenic filters with a pore size of 0.45 μm (Merck Millipore). Samples of these supernatants were applied immediately to target cells together with Polybrene (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: ... and SPPL2A and control siRNA (IDT) were transfected into two human cell lines (HMV-2/CVCL_1282 -Merck-, WM3211/CVCL_6797 -Rockland-) using Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... CIGB325 treated sample and background control were processed by the FASP method using Microcon 30k centrifugal ultrafiltration units (Merck, Darmstadt) [29] ...
-
bioRxiv - Genetics 2020Quote: ... 24 trpa1b F0 knockout and 24 scrambled-injected control larvae were placed in individual wells of a mesh-bottom 96-well plate (Merck), with the receiver plate filled with fish water ...
-
bioRxiv - Cell Biology 2021Quote: ... LECs (1.5 × 105) were transfected with 25 pmol of target siRNA or non-target siRNA (MISSION siRNA universal control#1, Merck) using Lipofectamine RNAiMAX (Thermo Fisher ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with MISSION® SiRNA oligomers or MISSION® siRNA Universal Negative Control #1 (Sigma Aldrich, Merck, USA) at a final concentration 20 nM ...
-
bioRxiv - Molecular Biology 2023Quote: ... Target cells without effectors served as a negative control and target cells incubated with 2% Triton X-100 (Merck-Sigma) served as positive control (maximum killing).
-
bioRxiv - Evolutionary Biology 2023Quote: ... CDK9 was immunoprecipitated with 15μg of an anti-CDK9 antibody (D-7, sc-13130, lot no # B1422) or control mouse IgG (12-371, Merck) in IP Buffer with 2X SDS buffer (100mM NaCl ...
-
bioRxiv - Molecular Biology 2024Quote: ... chromatin samples cross-linked by formalin were enzymatically shared and incubated with rabbit anti-AcH4 antibody or isotype control antibody (Merck) at 4 °C overnight ...
-
bioRxiv - Biophysics 2020Quote: ... Pseudotyped GFP expressing coronaviruses were harvested from the cell supernatant 24 hpi and concentrated using Amicon columns (Merck). Then the viruses were titrated in Vero E6 cells and stored at −80 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... the mice were inoculated with 100,000 SB28-GFP cells suspended in 2 µl of DMEM (Merck, Darmstadt, Germany) (glioblastoma mice ...
-
bioRxiv - Neuroscience 2020Quote: ... was transfected into COS-7 cells cultured in a well of 8 well chamber slide glass using GeneJuice Transfection Reagent (Merck Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967, Merck Millipore) according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were plated to ∼50% confluence in 6-well plates and 30pmol of siRNA against CTNNB1 (siCTNNB1) or non-targeting siRNA (siNT) (Dharmacon) were transfected using XtremeGENE™ siRNA Transfection Reagent (Roche, Merck) as per manufacturer’s instructions (for SW620 150pmol siRNA was used) ...
-
bioRxiv - Cancer Biology 2024Quote: ... or expressing the sequence of 4-1BBL (Vector Builder, pLV[Exp]-Hygro-EF1A>4-1BBL) using X-tremeGENE™ HP DNA Transfection Reagent (Merck).
-
bioRxiv - Bioengineering 2024Quote: ... Culture supernatants were harvested at 3 days post-transfection and filtered through a 0.45 μm filter (Merck Millipore, Burlington, MA, USA). All proteins were purified with a Talon metal affinity resin (Takara Bio USA ...
-
bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
-
bioRxiv - Cell Biology 2021Quote: ... The following siRNA sequences were used: Control siRNA: endoribonuclease-prepared siRNA pool (esiRNA) that that target multiple locations in the EGFP mRNA sequence (Merck; EHUEGFP), E1B-55k siRNA (I) ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 × 105 Cas9 TNG MKOS MEFs were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 polybrene (Merck-Millipore) for 4 hours ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours ...
-
bioRxiv - Microbiology 2020Quote: ... Additionally, 12 negative controls were included for the amniotic fluid sampling, aspirating ultra-pure, nuclease-free water (W4502, Sigma-Aldrich (Merck), Overijse ...
-
bioRxiv - Biophysics 2023Quote: ... Protein extracts (50 μg/lane) were resolved in 15% SDS-PAGE followed by immunoblotting using anti-GFP (AB3080P, Merck), anti-PrPC monoclonal antibody (anti-CD320 clone 6D11 ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck) according to manufacturer’s specifications ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck). After 48 h ...
-
bioRxiv - Neuroscience 2023Quote: ... For expression of GCaMP6s or GFP the mice were given 180mg/ml tamoxifen (Merck KGaA, Darmstadt, Germany, #T5648-1G) dissolved in corn oil (Merck ...
-
bioRxiv - Developmental Biology 2023Quote: Axolotl embryos of genotype tgSceI(Mmu.Prrx1:TFPnls-T2A-ERT2-Cre-ERT2; Caggs:loxP-GFP -loxP-mCherry)Etnka were treated with 4-OHT (Merck H7904) as described in 48 to permanently label connective tissue cells with mCherry ...
-
bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
-
bioRxiv - Molecular Biology 2023Quote: ... Lentivirus-containing supernatant was collected at 48h and 72h post-transfection and then concentrated using Amicon Ultra 100 kDa filters (Merck, Rahway, NJ). Lentiviral titter was calculated by the % of GFP+ cells using flow cytometry on serial dilutions of concentrated lentivirus stock ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... the supernatant was collected for three days after transfection and concentrated with a filter device (Amicon Ultra15 Centrifuge Filters, Merck, Cat# UFC910008).
-
bioRxiv - Cancer Biology 2023Quote: ... Viral particles were harvested from cells four days after transfection and concentrated using 50kDa Amicon Ultra-15 Centrifugal Filter Units (Merck, cat#UFC905024). Organoids were dissociated by mechanical disruption and dissolved in 250 μL ENR medium supplemented with Y-27632 and Polybrene together with the concentrated virus ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) and cells were fixed 5–6 days later ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
-
bioRxiv - Biochemistry 2020Quote: ... Tracer quality control and plasma metabolite analysis were performed with a LaChrom high-performance liquid chromatography (HPLC) system (Hitachi; Merck, Darmstadt, Germany) equipped with a Radiomatic 150TR flow-through radioisotope detector (Packard ...
-
bioRxiv - Developmental Biology 2022Quote: ... Measuring IVD-CE certified quality control materials (Chromsystems) and certified reference materials (BCR-576, BCR-577, BCR-578, IRMM, Merck/Sigma Aldrich) over five days ...
-
bioRxiv - Cancer Biology 2021Quote: ... β-actin which was used as loading control was detected using a rabbit polyclonal IgG against mouse β-actin (A2103, Merck, Darmstadt, GER) diluted 1000-fold ...
-
bioRxiv - Cell Biology 2022Quote: ... BDNFΔC-ter or Cytochrome-C (negative control which has a molecular weight and a pHi similar to those of BDNF; C2037; Sigma-Aldrich/Merck group) were injected on immobilized KEAP1 ...
-
bioRxiv - Biochemistry 2019Quote: ... On the next day the peptide and GFP were subject to concentration via ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck) and removal of the GFP and 3C protease ...
-
bioRxiv - Cell Biology 2019Quote: Hela cells were transiently transfected with pSpCas9(BB)-2A-GFP (PX458)(83) encoding sgRNAs targeting VPS41 using X-tremeGENE (Merck) according to the manufacturers recommendation ...
-
bioRxiv - Microbiology 2021Quote: ... CTR1 KO cells were generated by co-transfecting 293T cells with the pX458 vector carrying the Cas9 protease fused to GFP and a Sanger lentiviral CRISPR vector (Merck) carrying the BFP and a sgRNA against human CTR1 under the control of the U6 promoter (sgRNA ...
-
bioRxiv - Immunology 2023Quote: ... The tetramer (428 kDa) was purified from excess GFP-biotin (25 kDa) with a 100 kDa cut-off Amicon Ultra centrifugal filter (Merck). Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... we saturated the porous medium with a florescent dye (fluorescein sodium salt, from Merck, visible through a GFP filter cube) and recorded a large image of the entire structure ...