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Citations for Merck :
201 - 250 of 687 citations for hsa mir 301a 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then stained with a 1X crystal violet solution for 20 minutes at RT (2% crystal violet, Sigma; 20% ethanol; 4% formaldehyde, Merck). Plates were washed with clear water and plaques were counted manually ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR product was cloned into pFLAG-CMV5a (Merck) using restriction enzymes Bam HI and Kpn I ...
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bioRxiv - Developmental Biology 2022Quote: ... slices were washed three times with DMEM-F12 and grown on Millicell culture inserts (Merck) in cortical culture medium (DMEM-F12 containing B27 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed three times in PBS and counterstained with Hoechst 33342 (Merck, B2261). F-actin was detected using Alexa Fluor 488-conjugated phalloidin (ActinGreen 488 ReadyProbes ...
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bioRxiv - Cell Biology 2021Quote: ... After treatment for the indicated time the cells were fixed in 4% paraformaldehyde (Merck, 100496,) and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... washed four times in TBS+T and signals revealed by enhanced chemiluminescence (Merck Cat#WBLUF0100) on a ChemiDoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Secondary antibody and detection procedure was carried out following the manufacturer’s instructions (ECL™ Prime Western Blotting System, Merck; Darmstadt, Germany).
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bioRxiv - Microbiology 2023Quote: ... The PLA assay was performed according to the manufacturer’s instructions using the In Situ Detection Reagents Red (Catalogue Number: DUO92008, Merck, Darmstadt, Germany).
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bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with anti-human IFN-γ and anti-human IL2 antibodies for 40 min at RT in 0.2% saponin (Merck, Germany) in PBS (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Then, samples were incubated with streptavidin-phycoerythrin (30 min, RT) and before measurements on a Luminex MAGPIX® System (Merck-Millipore).
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Zoology 2022Quote: ... 6 μL 2X Kappa2G Fast PCR Ready Mix (Merck, Germany). Primer concentrations and volumes differed between the multiplex assays and are detailed in Table S1 ...
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bioRxiv - Molecular Biology 2019Quote: ... Integration fragments were generated by PCR using KOD polymerase (Merck) from standard vectors pUC19-EmFP ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed using KOD Hot Start DNA polymerase (Merck). PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... PCRs were performed with KOD hot-start polymerase (Merck #71086), using primers flanking the gRNA recognition site ...
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bioRxiv - Microbiology 2021Quote: ... cells and tissue were washed 3 times with phosphate buffered saline (PBS) + 0.02M glycine (Merck, K27662101) to remove fixative ...
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bioRxiv - Neuroscience 2021Quote: ... Stained sections were washed 4 times in PBS and then embedded using Aquatex (Merck Millipore, USA).
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bioRxiv - Microbiology 2020Quote: ... were confirmed by comparison of retention time to a commercial reference of (+)-valencene purchased (75056, Merck). To estimate the titre of α-cuprenene ...
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bioRxiv - Developmental Biology 2022Quote: ... The beads were then washed five times with binding buffer with 0.05% IGEPAL CA630 (Sigma, Merck). One tenth of the beads were eluted in loading buffer for Western blot purposes and the rest was processed for Mass Spectrometry analysis.
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bioRxiv - Biophysics 2020Quote: ... the mixture was washed with PBS buffer four times using 10kD cut-off concentrators (Merck-millipore). Labelled bacterial MVs were kept at 4°C and can be stored upto one week for further experiments.
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bioRxiv - Biochemistry 2021Quote: ... After binding beads were washed 3 times with binding buffer (PBS with protease inhibitor cocktail (Merck) and 1 mM Na3VO4 ...
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bioRxiv - Cell Biology 2021Quote: ... washed four times with PBS and permeabilized for 5 min with 0,5% Triton X-100 (Merck) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed three more times and affixed to slides with Fluoroshield histology mounting medium (Merck). Cell were viewed on am IX83 inverted microscope (Olympus ...
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bioRxiv - Cell Biology 2023Quote: ... all materials and equipment used were rinsed three times with filtered (0.1 μm filter – Merck Isopore) ultra-pure water prior to use ...
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bioRxiv - Developmental Biology 2023Quote: ... all materials and equipment used were rinsed three times with filtered (0.1 μm filter – Merck Isopore) ultra-pure water prior to use ...
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bioRxiv - Microbiology 2019Quote: ... Under these conditions nitrite was not detectable with a colorimetric test with a lower detection limit of 2 mg/L (MQuant test stripes, Merck, Darmstadt, Germany). Growth conditions and operation of the bioreactor containing ANME-2d archaea enriched from an Italian paddy field soil are described by Vaksmaa et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Immunoreactive bands were detected using iBright CL1000 chemiluminator or LAS 3000 (Fujifilm, Tokyo, Japan) with an enhanced chemiluminescent substrate for the detection of horseradish peroxidase (HRP; Merck, Darmstadt, Germany). The intensity of the RAS band was quantified using ImageJ software and normalized to the intensity of the GAPDH band.
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bioRxiv - Neuroscience 2022Quote: ... followed by 10 min incubation at RT with 50% (v/v) lead citrate solution in decocted H2O containing 80 mM lead citrate (Merck, Darmstadt, Germany) and 0.12 M trisodium citrate (AppliChem ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Immunology 2021Quote: ... The plates were finally washed 5 times with HSWB before adding 100 µL of TMB (Merck, Germany) to each well ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryoid bodies were then washed three times in PBST and incubated with 1μg/ml DAPI (Merck, USA) in PBS for 45 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed three times with PBS and blocked with 5% normal donkey serum (NDS) (Merck-Millipore), 0.1% Triton X-100 in PBS for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed another 2 times and permeabilised with 0.1% Triton™ X-100 (Merck, UK) diluted in PBS and incubated at room temperature for 5 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... before Rab8a was washed 3 times with PBS in an Amicon filter (Merck Millipore, 10 kDa NMWL). Incorporation of label was confirmed by MS.
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bioRxiv - Genetics 2021Quote: ... worm pellets were flash-frozen three times before RNA extraction in TRI Reagent® (Merck, Darmstadt, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Plates were washed 5 times with PBS-T and 100µl of 3,3′,5,5′-Tetramethylbenzidine (TMB) (Merck Millipore) equilibrated at room temperature were added ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction buffer was exchanged to the lysis buffer five times by Amicon Ultra 10K (Merck Millipore). Then ...
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bioRxiv - Cell Biology 2024Quote: ... they were washed four times for 3 minutes with PBS and mounted in Mowiol reagent (81381, Merck). The image acquisition was done on a Zeiss LSM880 confocal microscope running the software Zeiss ZEN2.3 SP1 FP3 (black ...
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bioRxiv - Microbiology 2021Quote: ... and PCR of the spike was performed using KOD polymerase (Merck). For next generation sequencing RNA from virus-containing samples were extracted using the QIAsymphony DSP Virus/Pathogen mini kit (Qiagen) ...
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bioRxiv - Bioengineering 2020Quote: ... AGPAT1 was then amplified by PCR with KOD DNA polymerase (Merck) using primer pair 5’-CACCATGGATTTATGGCCTGGTGC-3’ & 5’-TCATCCTCCTCCACCTGG-3’ ...
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bioRxiv - Genomics 2022Quote: ... Plasmid concentrations were measured by quantitative PCR with SYBR green (Merck) and primers annealing to the origin of replication site of the PCUP1-Sup35N-Aß42 plasmid at 58 C for 40 cycles (primers MS_05-06 ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were ligated into the plasmid pET24a (+) (Merck, Germany) at NdeI/XhoI restriction sites ...
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bioRxiv - Genetics 2022Quote: ... Incubation with appropriate secondary HRP-labelled antibody (less than 1h) was followed by detection with Immobilon western chemiluminescent HRP substrate (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biochemistry 2022Quote: ... Blocked membranes were incubated (1 hour, 1/5000 dilution, RT) with mouse anti-phosphotyrosine (clone 4G10) directly conjugated to HRP (4G10-HRP; Merck/ Millipore, RRID: AB_310779), or unlabelled 4G10® Platinum (Merck/ Millipore ...