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Citations for Merck :
151 - 200 of 239 citations for Yellow Fever virus lysate 17D strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was then mixed with 1 volume of phenol-chloroform-isoamylalcohol (Merck cat #P2069), and centrifuged for at maximal speed in a table top centrifuge at 4°C for 15 min ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were separated by SDS-gel electrophoresis and transferred to PVDF membranes (Merck Millipore). Immunoblotting was performed using the following antibodies from Cell signaling ...
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bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
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bioRxiv - Pathology 2023Quote: ... 48h after transfection rAAV8 vectors were harvested from cell lysate and treated with Benzonase (Merck) at 100U/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysate was incubated in the presence of BG4 (Merck, Milan, Italy, MABE817, Lot: 3534080). Anti-FLAG antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (MDA, A549 and HCT) were then infected with the produced virus using 2μg/ml polybrene (Merck, Darmstadt, Germany, #TR-1003-G). Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen ...
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Microbiology 2022Quote: ... ljungdahlii strain and its derivatives was carried out in 125 mL serum bottles (Sigma-Aldrich, Merck Life Science, Gillingham, UK) with a working volume of 30 mL ...
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Biochemistry 2023Quote: ... equivalent masses of total lipid fraction from each strain were spotted onto a high-performance TLC (HPTLC) silica gel 60 plate (Merck) with a Camag ATS4 apparatus ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA and RNA were removed from the cell lysate using benzonase (1 U/ml, Merck Millipore) for 10 min on room temperature (RT) ...
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bioRxiv - Immunology 2020Quote: ... Flag pull down was performed incubating cleared cell lysates with Anti-flag M2 affinity gel (Merck) during 2h ...
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bioRxiv - Neuroscience 2021Quote: ... 400 μl cell lysate were incubated with 4 μl HTT MAB2166 rabbit primary antibody (Merck, #MAB2166) for 2 hours at 4° C on a rotating device ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: The phage lysate was concentrated using either Centricon (Centricon® Plus-70 Centrifugal Filter Units, Merck) or Amicon (Amicon® Ultra-15 Centrifugal Filter Unit ...
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bioRxiv - Immunology 2024Quote: ... the cell lysates were incubated with 15 μl of SA beads (Invitrogen, 65601; Merck Millipore, 69203) for 6 hours at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 2 ml of each recipient and donor strains were combined (three mating pairs in total) and filtered through sterile 0.22 µm membranes (Merck Millipore, GSWP02500), which were incubated over TSA plates for 8 days at 22°C ...
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bioRxiv - Neuroscience 2020Quote: ... were expressed as fusion proteins with 6x polyHis tail at the C-terminus in E.coli strain BL21(DE3) (Merck-Novagen, Darmstadt). After harvesting ...
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bioRxiv - Synthetic Biology 2022Quote: ... cerevisiae strains for preparation of competent cells were cultivated in YPD medium consisting of 10 g/L yeast extract (Merck Millipore), 20 g/L peptone (Difco ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were resolved by SDS/PAGE under reducing condition and transferred to PVDF membranes (Merck Millipore). After blocking ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of lysate was incubated overnight with 20µl anti-Flag M2 magnetic Dynabeads (Sigma-Aldrich/Merck) under shaking ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared via centrifugation and loaded on two 5 mL HiTrap Heparin HP (Cytiva, Merck) columns (connected in series ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were sonicated for 1 min and 20 sec and incubated with 75 U/mL Benzonase (Merck, Darmstadt ...
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bioRxiv - Immunology 2020Quote: ... Lysates were further cleared by sterile filtration employing a 5 µm filter unit (Merck Millipore, Darmstadt, Germany). The first column contained 1 mg of W6/32 antibody coupled to sepharose ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was sonicated in chilled water bath and treated with 25 units of Benzonase (Merck Millipore) for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride membranes (PVDF, Merck Millipore) for immunoblot analysis ...
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bioRxiv - Microbiology 2020Quote: ... Proteins in each lysate were resolved by SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck). The blots were incubated with the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using the ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Merck).
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bioRxiv - Genetics 2022Quote: Whole-cell protein lysates were extracted in modified radioimmunoprecipitation assay buffer supplemented with protease inhibitors (#5892970001, Merck) and phosphatase inhibitor cocktail (#4906837001 ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... cell lysates (500 μg) were subjected to immunoprecipitation with LRP1α (1 μg, Cat#: L2295, Merck, Rahway, NJ), HA (0.2 μg ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated overnight with 30 μL ChIP-grade Protein G agarose beads (Merck Millipore, Germany) and 10 µg DNMT1 ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Genomics 2023Quote: ... These assessments were conducted on 7-day old Foc strains cultured on petri dishes with potato dextrose agar (PDA; Merck, Darmstadt, Germany). An approximately 5 mm piece of the actively growing colony from each isolate was placed on PDA plates and incubated at 27°C ...
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bioRxiv - Cell Biology 2021Quote: ... antibody complexes were purified by incubation of lysate with Protein A Sepharose® beads (Merck, GE17-5138-01) for 3h rotating at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... beads were incubated with astrocyte lysate buffer supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) for one hour at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were incubated overnight under rotation at 4°C with ANTI-FLAG® M2 Affinity Gel beads (Merck) to pull down FLAG-containing SNAP-GLP-1R ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysates were subjected to SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Merck Millipore; Darmstadt, Germany). After blocking the membranes with 5% skim milk (Morinaga ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were harvested in 4°C RIPA buffer with protease inhibitor (cOmplete, EDTAfree Protease Inhibitor Cocktail, Merck) and homogenised by sonication for 30 sec ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mg of the total lysates were mixed with 1mL 0.1 mg/mL pepsin in 0.5M acetic acid (Merck) and put at 4°C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell pellets were lysed by French pressing and the lysates clarified by centrifugation at 15,000 g followed by filtration using 0.22 μm Millex syringe filters (Merck). Protein purification was performed with an ÄKTA start chromatography system (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were normalised across samples and were added to 100 μl anti-FLAG M2 affinity gel (Merck) Micro Bio-Spin columns (Bio-Rad ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... The lysates underwent four cycles of freeze and thaw followed by benzonase (Emprove expert benzonase endonuclease, Merck #101695) digestion of non-encapsidated DNA at 37 °C for 30 min with gentle shaking using 125 U of enzyme per mL of lysate ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates (25 µL) and bovine serum albumin (BSA) protein standards (0-1 mg/mL BSA (Merck, Germany) in resuspension buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Total 2 mg lysate (1 ml) was incubated with 10 µl of anti-FLAG M2 Magnetic Beads (Merck), which was pre-washed twice with IP buffer ...
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bioRxiv - Cell Biology 2024Quote: ... whole cell lysates were prepared from two pellets per group using Phosphosafe Extraction Reagent (Merck Millipore, Darmstadt, Germany) containing 1 mM Pefablock SC (Sigma Aldrich ...