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Citations for Merck :
101 - 150 of 239 citations for Yellow Fever virus lysate 17D strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Single colonies of the propagating strains were inoculated into LB medium (Lysogeny Broth, Merck, Darmstadt, Germany) and incubated at 37°C shaking at 170 rpm until reaching the exponential phase (approximately five hours) ...
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bioRxiv - Microbiology 2021Quote: ... One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 × 106 SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 293T or 293GP virus producing cells were transfected using the GeneJuice reagent according to the manufacturer’s recommendations (Merck Millipore). Two days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... One milliliter of the virus supernatants was directly overlaid on the cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Genomics 2023Quote: ... The transduction was performed by adding the virus particle directly onto the cells supplemented with 6μg/mL polybrene (Merck). The cells were refreshed 24h after transduction and the antibiotics (puromycin/blasticidin ...
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bioRxiv - Plant Biology 2020Quote: ... the strain was grown on solid King’s medium B (Pseudomonas agar F, Merck KGaA, 64271 Darmstadt, Germany) supplemented with 50 μg mL−1 rifampicin at 25°C in the dark for 3 days ...
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bioRxiv - Systems Biology 2020Quote: ... albicans strain SC5314 (ATCC MYA-2876) were cultivated on Tryptic Soy Agar (TSA) ReadyPlatesTM (Merck, Darmstadt, Germany) at 37°C overnight ...
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bioRxiv - Microbiology 2021Quote: 10His-tagged MA gene was expressed in Escherichia coli (E. coli) strain BL21 (DE3) (Merck, Darmstadt, Germany). The 10His-tagged MA protein was initially purified with nickel-NTA affinity column chromatography followed by TEV cleavage ...
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bioRxiv - Microbiology 2023Quote: ... The strains were first cultivated in separate pure cultures overnight on Tryptic soy agar (TSA; Merck, Germany) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... coli RIL strains carrying the appropriate plasmid were grown in 1 l Luria-broth (LB) (Merck Millipore) supplemented with kanamycin (100 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... the pUL21 gene was amplified from virus stock by PCR with the oligonucleotide primers 5′-ATGGAGCTTAGCTACGCCAC-3′ and 5′-TTTATTGGGGTCTTTTACACAGACTGTC-3′ using KOD polymerase (Merck) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For detection of particle incorporation virus supernatant was further concentrated by centrifugation at 4°C for 2h at 21000xg on 5% Optiprep (Merck), the supernatant was removed and pellet was resuspended and subjected to Western Blot analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration of each lysate was determined using a BCA assay (Merck, 71285).
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bioRxiv - Microbiology 2021Quote: ... 0.5 ml of phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 ug/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Microbiology 2021Quote: ... 500 μl of the phage lysate was treated with DNaseI (Merck cat #11284932001) added to a final concentration of 20 μg/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was loaded overnight onto a 5mL HisTrap HP column (Cytiva, Merck) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Cleared cell lysate was filter with 0.45 µm sterile filter (Millipore Merck, Schwalbach) and loaded onto a 5 ml HiTrap Chelating HP column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2022Quote: The cell lysate was loaded on 10 mL D-mannose-agarose resin (Merck) pre-equilibrated with Buffer A ...
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bioRxiv - Synthetic Biology 2022Quote: ... the cell lysate was loaded on 10 mL D-mannose-agarose resin (Merck) pre-equilibrated with Buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared using 1X RIPA buffer containing protease (#539131, Merck Millipore) and phosphatase (#524629 ...
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bioRxiv - Immunology 2023Quote: ... Colony-forming units (CFU) were enumerated in tumour lysates on LB agar (Merck) using standard microbiological techniques.
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate was incubated with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: Caspase-9 activity was measured in liver lysate according to manufacturer’s protocol (Merck Caspase-9 Colorimetric Activity Assay Kit APT139 ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate underwent immunoprecipitation with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: Extraction of RNA from cell lines/strains was carried out using TRI Reagent (Merck Life Sciences, Dorset UK) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... One strain tube thawed rapidly at 37 °C and cultured in 10 ml Brain heart infusion (BHI - Merck) at 37 °C over 24 hours ...
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bioRxiv - Genomics 2021Quote: ... rosea strains was inoculated at edge of a 9-cm-diameter potato dextrose agar (PDA; Merck, Kenilworth, NJ) Petri plate covered with a durapore membrane filter (Merck ...
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bioRxiv - Microbiology 2022Quote: ... was applied onto the dried treatment (10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). After 5 minutes at room temperature 150μl of DMEM (high glucose ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Microbiology 2020Quote: ... 500 μl of the phage lysate was treated with DNAse-I (Merck cat #11284932001) added to a final concentration of 20 μg/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... The lysates were separated through SDS-PAGE and transferred to nitrocellulose membranes (Merck Millipore), blocked in 5% milk in TBS with 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2022Quote: ... 500 µl of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 µg/ml and incubated at 37C for 1 hour to remove bacterial DNA ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg chromatin lysates were incubated with 50 µl FLAG M2 beads (Merck A2220) for 2 h at 4°C with rotation ...
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bioRxiv - Developmental Biology 2022Quote: ... the lysate was concentrated on an Amicon Ultra Centrifugal Filter (Merck Millipore; Billerica, MA), 10μl of the concentrated lysate was resolved on a 4-12% NuPAGE Bis-Tris gel and transferred onto a PVDF membrane using the iBlot system (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... The total lysate was exchanged into PBS buffer using centrifugal filter devices (Merck, UFC201024) according to manufacturer protocols.
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bioRxiv - Microbiology 2022Quote: ... 500 µL of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 mg mL−1 and incubated at 37°C for 1 h to remove bacterial DNA ...
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bioRxiv - Biochemistry 2023Quote: ... Resulting crude lysates were then sonicated for 5 min in an ultrasonic bath (Merck) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 ml of phage lysate was treated with DNase-I (Merck catalogue no. 11284932001) added to a final concentration of 20 µg/ml and incubated at 37 °C for 1 h to remove bacterial DNA ...
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bioRxiv - Genetics 2023Quote: ... 500 µl of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 mg ml−1and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Neuroscience 2020Quote: ... individual COs were transferred to an Eppendorf tube containing CMM medium with 1:100 of virus containing medium and 5µg/ml Polybrene (Merck; TR-1003-6) and incubated over-night ...
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bioRxiv - Molecular Biology 2021Quote: hEnSC were seeded at a confluency of 105 cells/well in 6 well plates and 1.5 ml of concentrated virus added to each well in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... hTERT cells were transduced with 1.5 ml of concentrated pMY-SUZ12-FLAG-IRES-bls virus in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
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bioRxiv - Plant Biology 2020Quote: ... The strains were routinely grown on solid King’s Medium B (KMB; Pseudomonas agar F, Merck KGaA, 64271 Darmstadt, Germany) supplemented with rifampicin 100 μg/mL at 24°C for 4 days ...
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bioRxiv - Developmental Biology 2023Quote: ... F1 generation of 8-week female hybrid mice (generated via C57BL6 female and CBA/W strain crosses) were intraperitoneally injected with 7.5IU of PMSG (pregnant mare serum gonadotrophin; Merck) and reinjected after 48 hours 7.5IU hCG (human chorionic gonadotrophic hormone ...
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bioRxiv - Plant Biology 2024Quote: A pTYB2-based vector for the expression of PSM polypeptides and a pCDF-based vector that contained heme oxygenase 1 and phytochromobilin synthase genes for the synthesis of PΦB (Muramoto et al., 1999; Kohchi et al., 2001) were transformed into the Escherichia coli Rosetta 2 strain (Merck). E ...
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bioRxiv - Microbiology 2024Quote: ... cerevisiae strains was investigated in SC -Ura medium supplemented with 1.5 ppm prothioconazole dissolved in 50 % DMSO (Merck, NJ) and measuring the OD600 in SpectraMax Gemini™ XPS/EM microplate reader (Molecular Devices ...