Labshake search
Citations for Merck :
601 - 650 of 1496 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Obtained pellets were washed in HBSS and then resuspended in DMEM/F12 medium supplemented with 15% of foetal bovine serum (FBS, Sigma Aldrich/Merck, Poznan, Poland) and 1% P/S solution ...
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bioRxiv - Biochemistry 2019Quote: ... The fractions corresponding to the single peak were pooled and concentrated using an Amicon Ultra −15 centrifugal filter (3 kDa, Merck Millipore Ltd. Ireland). Copper-(I ...
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bioRxiv - Biophysics 2019Quote: ... ES products from all three days were thawed at 4 °C and pooled to be concentrated 720 times with Amicon® Ultra-15 Centrifugal Filter Unit 10 kDa cut-off (Merck, cat#UFC901024). The concentrate was used for EV separation.
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bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... The active fractions post-purification was pooled and concentrated using 30 kDa cut-off size membranes of Amicon-Ultra-15 (Merck Millipore, Bangalore, India). Plasmid purification and gel purification kits were obtained from Qiagen (Hilden ...
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bioRxiv - Plant Biology 2019Quote: ... KG, Steinfurt, Germany), and then centrifuged (3000 g, 40 min) through Amicon®Ultra-15 30K filter units (Merck Millipore Ltd., Carrigtwohill, Ireland) to concentrate the recombinant protein to a final volume of 250 μl ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated using Amicon Ultra-15 centrifugal filter unit with nominal molecular weight limit of 10 kDa (Merck Millipore, Burlington, MA), loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... Whole purified His-MBP-ApoE4M was concentrated to a final concentration of ∼ 8 mg/ml using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). The crystallization was performed in Easy-Xtal 15-well crystallization plates by a hanging drop vapour diffusion ...
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bioRxiv - Immunology 2023Quote: ... The eluted proteins were buffer exchanged into PBS and then concentrated using Amicon® 30 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9030).
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bioRxiv - Cancer Biology 2023Quote: ... Each imaging session was performed 15 minutes after intraperitoneal injecting D-Luciferin infusion (working concentration 15mg/ml, dose 10ml/Kg, MilliporeSigma, Merck KGaA, Darmstadt, Germany), considering the eLuciferase average flux (photons/second ...
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and concentrated to 500 μl using Amicon Ultra-15 10K MWCO centrifugal filters (Merck Millipore UFC901008; final LDAO = 2%) and further purified by size-exclusion chromatography as described for human VDAC constructs but with 20 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant containing the asymmetric vesicles was then concentrated to < 500 μl using 15 ml-Amicon centrifuge filters (Merck, 100 kDa cut-off) at 5000 g ...
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bioRxiv - Developmental Biology 2023Quote: ... Leuplin for Injection Kit), a micro-infusion pump (ALZET Osmotic Pumps, iPRECIO SMP-200) with 15 IU/kg human FSH (hFSH, Merck Biopharma, Gonal-f) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Immunology 2023Quote: ... The resulting Fabs were buffer exchanged into PBS and then concentrated down using Amicon® 10 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9010). Selected fractions resulting from the size-exclusion chromatography were pooled together and concentrated again for later use.
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bioRxiv - Neuroscience 2023Quote: ... filtered to remove cell debris and particles were concentrated (30-fold) via low-speed centrifugation (Amicon Ultra-15, Ultracel-100; Merck Millipore; Cat# UFC9100) before aliquoting and storage at −70°C ...
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bioRxiv - Microbiology 2023Quote: ... Cell-free extracts were then resolved by SDS-PAGE using 12.5%-15% polyacrylamide gels and subsequently electro-blotted onto a 0.45 μm polyvinylidene difluoride (PVDF) Immobilon®-P membrane (Merck Millipore, Sigma Aldrich) at 100 V for 90 minutes ...
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bioRxiv - Biochemistry 2023Quote: Purified NanoLuc was concentrated to a final concentration of ∼10 mg/mL using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). Concentrated NanoLuc was mixed with a 4 molar excess of FMZ non-oxidizable analogue azaFMZ (stock solution of azaFMZ was 10 mM in isopropanol) ...
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bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
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bioRxiv - Neuroscience 2024Quote: ... The filtered supernatant was concentrated ×20 using an Amicon® Ultra-15 centrifugal filter device (molecular weight cut-off 100 kDa, UFC910024, Merck Millipore Ltd.), which was centrifuged at 4,000×g for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used in this study were: mouse monoclonal antibodies against Centrin (1:500 for immunofluorescence; Merck Millipore catalogue number 04-1624 ...
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bioRxiv - Immunology 2024Quote: ... and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP) from Merck Millipore was used at a dilution of 1:3500 ...
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bioRxiv - Microbiology 2020Quote: The crude ethylacetate extract of ALR-2 was purified via column chromatography using a LiChroprep RP-18 (15-25 μm) silica gel column (Merck, EMD Millipore Corporation, USA) and chloroform:methanol (ratio 4:1 ...
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bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
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bioRxiv - Bioengineering 2021Quote: ... was centrifuged at 200 g for 5 mins followed by 2000 g for 10 min and concentrated from 50 ml to about 500 μl using Amicon® Ultra-15 Centrifugal Filter concentrators with a 3k cutoff (Merck Millipore, Solna, Sweden). The IZON EV original columns were rinsed with 15 ml PBS (0.22 μm filtered ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS- PAGE and those containing pure tag free CBX8 protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer consisting of 20mM MES pH 6.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer containing 25 mM Tris ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2 ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... All viromes were concentrated from 12 ml down to approximately 200 μl SM-buffer using the Amicon® Ultra-15 Centrifugal Filter Devices 100K (Merck Millipore, Darmstadt, Germany), following manufactures recommendations ...
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bioRxiv - Developmental Biology 2020Quote: ... and in vitro culture in mineral oil covered drops (~20 μL/ ~15 embryos) of commercial KSOM (EmbryoMax® KSOM without Phenol Red; Merck, MR-020P-5F), conducted as previously described (Mihajlovic et al. ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were buffer exchanged into PBS and then concentrated down using Amicon® 100 kDa or 10 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9100 & UFC9010), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... was used to plot elution profiles of the protein standard mix Supelco® 69385 Protein Standard Mix 15-600 kDa (Sigma-Aldrich/Merck, Darmstadt, Germany), and the analyzed protein samples.
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bioRxiv - Cancer Biology 2023Quote: ... EVs were collected from 1st to 5th fractions by using an Izon Automatic Fraction Collector (AFC) and concentrated in Amicon Ultra 15 filtering units (Merck Millipore, Cat. No. UFC910024) by centrifugation at 3.000g for 20 min on a 5810R benchtop centrifuge (Eppendorf) ...
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bioRxiv - Biochemistry 2023Quote: ... AnfH2 eluted in a clear peak at around 205 mL and was subsequently concentrated using an Amicon® Ultra-15 Centrifugal Filter Unit (molecular weight cut off = 30 kDa; Merck Millipore, Billerica, USA). Protein yields for both nitrogenase component fractions were determined using the Quick Start™ Bradford 1x Dye Reagent (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged to remove cell debris and concentrated with Amicon Ultra-15 Centrifugal Filters (10 kDa molecular weight cut-off; Merck Millipore Ltd, Carrigtwohill, Ireland). Aliquots were stored at −80°C until usage.
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 30 μL from each sample was subjected to electrophoresis on 8% or 15% polyacrylamide gel for ARAP1 and ADAP1 each and transferred into an Immobilon®-P transfer membrane (Merck Millipore Ltd., Germany). Membranes were blocked with 5% skim milk (Nacalai Tesque ...
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bioRxiv - Microbiology 2019Quote: ... Antibody staining was performed using EV-A71 monoclonal antibody MAB979 (Merck, USA; 1:2500 dilution in 1 % BSA) for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated overnight at 4aC with the primary antibody (Anti-DNA-RNA Hybrid Antibody, clone S9.6; Merck Millopore MABE1095), washed three times with PBS-T (0.05% Tween-20 in PBS) ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: anti-APC mouse monoclonal antibody (1:200; Merck Millipore, Burlington, MA, USA), anti-GFAP-488 mouse monoclonal antibody (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... The antibodies used were: rabbit anti-tyrosine hydroxylase antibody (1:1000, AB152, Merck Life Science SLU, Darmstadt, Germany), chicken anti-GFP (1:500 ...
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bioRxiv - Cell Biology 2020Quote: Mouse monoclonal antibody for vinculin (Merck, V9131), rabbit polyclonal antibody recognizing the C-terminal tail of the NMIIA heavy chain (Biolegend ...
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bioRxiv - Molecular Biology 2020Quote: ... using an anti-puromycin antibody (Merck #MABE343). Puromycin incorporation was measured using Image Studio (version 5.2) ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies against TPRX1 1:200 (Merck, HPA044922) and H3.X/Y 1:200 (Active Motif ...
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bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-acetyl Histone H3K9 antibody (Merck ABE18), Anti-Dimethyl Histone H3K9 antibody (Merck 05-1249) ...
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bioRxiv - Neuroscience 2020Quote: ... polyclonal rabbit antibody (catalog no. 657012, Merck) was used as the primary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... anti-neurofilament antibody (Merck KGaA, Darmstadt, Germany) and an α-synuclein antibody (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit Anti-Neurofilament 200 antibody (Merck, #N4142), rabbit Anti-BetaIII-Tubulin antibody (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-nuclei antibody (Merck, 1:100), mouse anti-STEM101 antibody (Takara Bio Inc. ...
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bioRxiv - Genomics 2022Quote: ... CTCF (07-729) antibody was from Merck Millipore ...