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Citations for Merck :
501 - 550 of 1496 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Mouse anti-NeuN antibody (Merck Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... H3K27ac antibody (07-360; Merck), H3K27me3 (9733S ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then fixed with 4% p-formaldehyde (PFA) during 15 min and stained with DAPI (5 min, 1 μg/ml in PBS; Merck-Millipore).
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bioRxiv - Neuroscience 2021Quote: ... followed by 15 minutes in 95% and 100% ethanol and 5 minutes in hexamethyldisilazane (HMDS, Sigma-Aldrich, Merck, St Louis, USA). Samples were left to air-dry overnight in a biosafety cabinet prior to mounting on electron microscope stubs covered with carbon tape ...
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bioRxiv - Plant Biology 2021Quote: ... The eluate containing the nanodiscs was concentrated to 1 ml with the use of an Amicon Ultra-15 10K device (Merck Millipore) and then mixed with 10 ml of translation buffer containing 30 mM HEPES-KOH (pH 7.8 ...
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bioRxiv - Systems Biology 2021Quote: ... Excess FSBA reagent was removed by ultracentrifugation with 15 mL 10 K MWCO Amicon® Ultra-4 centrifugal filter units (Merck) at 3,500 × g at RT ...
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bioRxiv - Cell Biology 2021Quote: ... EVs containing fractions from 24 h conditioned medium were concentrated using a 100 kDA filter (Amicon Ultra-15, Millipore, Merck, Irealnd) and total RNA was isolated as previously described ...
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bioRxiv - Systems Biology 2022Quote: ... UK) previously coated with calf-skin collagen (15 μg/cm2 and fibronectin 3 μg/cm2; both Merck Life Science UK Ltd.). The permeability of hCMEC/D3 cell monolayers to 70 kDa FITC-dextran (2 mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: The CM was then filtered using 0.22 µm PES filters (FisherBrand) and concentrated 220 x down to 500 μL with Amicon Ultra-15 100K MWCO Centrifugal filters (Merck Millipore).
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bioRxiv - Plant Biology 2022Quote: A hole punch was used to obtain approximately 100 mg of leaf tissue from each sample (15 biological replicates per condition) and the total RNAs were extracted using the Spectrum Plant RNA kit (Merck, Spain). RNAs were quantified using the NanoDrop ND-1000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: IACS-010759 (from the Institute for Applied Cancer Science at MD Anderson)15 and 4-hydroxytamoxifen (Merck Life Science, Darmstadt, Germany) were dissolved in DMSO and ethanol ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... The sample that has passed through the sucrose was collected and concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). For infectious SARS-CoV-2 immunization ...
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bioRxiv - Biochemistry 2021Quote: ... pooled and concentrated to roughly 0.5 – 1 mL by ultrafiltration spin units Amicon® Ultra–15 10 kDa MWCO (Merck-Millipore). Buffer was exchanged against storage buffer (40 mM Hepes (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... All patients underwent ovarian puncture (OPU) of follicles ≥15 mm in size after 36 h of hCG administration (Ovitrelle, Merck Serono).
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bioRxiv - Biochemistry 2020Quote: The pure protein recovered after HPLC purification in 1% acetic acid was concentrated in Amicon Ultra-15 (cut-off 3 kDa) centrifugal filter units (Merck Millipore) to reach the final protein concentration of 1 mM ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Cell Biology 2020Quote: ... from affinity purification were pooled and buffer exchanged using Amicon™ Ultra-15 Centrifugal Filter Unit (10 kDa cutoff; Merck™) in 10 mM HEPES-NaOH buffer ...
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bioRxiv - Physiology 2021Quote: ... 10 mL of CM or qEV fractions 6-10 diluted in PBS were loaded on 15 mL Amicon Tubes with a cut-off of 100 kDa (Merck, Germany) and spun for 1 hour RT at 5000 xg on a FA-45-6-30 fixed angle rotor ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions corresponding to the elution peak were selected and concentrated to 2-50 mg/mL through the use of Amicon® Ultra-15 Centrifugal Unit (Merck), frozen and stored at −80°C for downstream experiments ...
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bioRxiv - Immunology 2022Quote: ... Supernatant from doxycycline-induced or uninduced cells (40mL per condition) was collected and concentrated to 500 μL using Amicon Ultra-15 filters with 10 kDa cutoff (Merck Millipore). The concentrated samples were loaded on a Superose 6 10/300GL column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... The virus-containing fraction was extracted from the tubes and AAVs were then concentrated in Dulbecco’s Phosphate Buffered Saline (D-PBS) + 5% sucrose using Amicon Ultra-15 (100K) filter units (Merck Millipore UFC910024) at 3220 g ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified protein was dialyzed overnight in 25 mM Tris-Cl pH 7.5 using SnakeSkin®ialysis tubing (7 kDa molecular weight cutoff) and concentrated using Amicon™ Ultra-15 Centrifugal Filter Units (30 kDa molecular weight cutoff) (Merck) according to the requirements of the experiments.
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Microbiology 2022Quote: ... was applied onto the dried treatment (10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). After 5 minutes at room temperature 150μl of DMEM (high glucose ...
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bioRxiv - Neuroscience 2022Quote: ... Virus particles collected from the cell lysate and supernatant were purified by ultracentrifugation with OptiPrep (1114542, Axis-Shield) and concentrated by ultrafiltration with Amicon Ultra-15 Centrifugal Filter Unit (UFC903024, Merck Millipore). The physical titer of the virus vector (genome copies (gc)/mL ...
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bioRxiv - Plant Biology 2023Quote: ... and concentrated to 2 mg chlorophyll mL-1 using a 100-kDa molecular-weight cut-off centrifugal filter unit (Amicon Ultra-15, Merck Millipore). A 3 µl volume of the sample was applied to a glow-discharged holey carbon grid (GIG ...
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Neuroscience 2023Quote: ... quality of the elution was assessed by loading 50µl of the resulting elution on a pre-cast 4-15% gradient gel (Merk, MP41G15) and incubated with SYPRO RUBY (Merck, S4942) protein gel stain ON at RT ...
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bioRxiv - Immunology 2023Quote: ... the supernatant was concentrated by centrifugation at 1000 g at 4 °C through Amicon® Ultra 15 mL Centrifugal Filters (100 K; Merck). Finally ...
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bioRxiv - Bioengineering 2023Quote: ... Protein-containing fractions were pooled and buffer exchanged with SnakeSkin™ dialysis tubing (10 MWCO, Pierce, 68100) followed by additional dialysis and concentration with Amicon Ultra-15 50 kDa centrifugal units (Merck, UFC905008). Purified protein was conjugated to AlexaFluor647 (Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... the eluate was reduced to approximately 40–50 ml and then concentrated using an Amicon Ultra-15 10 kDa molecular mass cut-off spin filter (Merck Millipore). Final EV preparations were kept in sterile filtered PBS for immediate use or sterile PBS-HAT buffer50 for storage at -80°C.
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bioRxiv - Developmental Biology 2023Quote: ... a micro-infusion pump (iPRECIO SMP-200, ALZET Osmotic Pumps) with 15 IU/kg human follicle-stimulating hormone (hFSH, Gonal-f; Merck Biopharma) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 50 μg of proteins were separated by 10% or 15% SDS- polyacrylamide gel (SDS-PAGE) and transferred to a nitrocellulose membrane (Merck, HATF00010). To detect the CXCL16 and CXCL8 secreted in the culture medium ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted fractions were concentrated and dialyzed against PBS using Amicon Ultra-15 Centrifugal Filter Units with a 3 kDa MWCO (Merck Millipore). Endotoxins were removed using High Capacity Endotoxin Removal Spin Columns (Pierce) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatants were transferred to new tubes and incubated overnight at 4 °C with 15 µl of Anti-FLAG® M2 Magnetic Beads (Merck) with gentle mixing ...
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bioRxiv - Molecular Biology 2023Quote: ... filtered cell culture supernatant containing infectious HCV was concentrated 50x using 100 kDa Amicon Ultra-15 centrifugal filter units (Merck Millipore) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... the stabilized pellets were enzymatically lysed for 30 min in a lysozyme solution (15 mg/ml in TE buffer; L4919 Sigma-Aldrich, Merck) combined with 1:10 (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... and dialyzed against cold sterile PBS using an Amicon Ultra-15 Centrifugal Filter Unit with a 3 kDa MWCO (Merck Millipore). The resulting product was then passed through a 0.22 µM filter.
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bioRxiv - Molecular Biology 2024Quote: Serum-free media from transfected HEK293T was concentrated to about 2 ml with Amicon Ultra-15 Centrifugal Filter 10 kDa MWCO (Merck Millipore) at 3,000 rcf for 15-20 min ...
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bioRxiv - Microbiology 2023Quote: ... The flow-through was sterile filtered and concentrated to a final volume of 7 ml using Amicon Ultra-15 Centrifugal filter concentrator (Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... The elution buffer was further exchanged with Thermo Scientific BupH Tris Buffered Saline (TBS) by using Amicon Ultra-15 Centrifugal Filter Units with Ultracel-10 membrane cutoff (Merck Millipore) and protein concentration was measured with a Qubit 2.0 fluorometer.
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were mouse monoclonal antibodies against actin (1:10000, Merck Millipore, MAB1501R), synaptophysin (1:5000 ...
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bioRxiv - Microbiology 2019Quote: ... polyclonal α-Hpf antibody (1:5000) [69] or monoclonal anti-puromycin antibody (1:10.000, Merck) and HRP-conjugated anti-mouse or anti-rabbit antibodies (1:10.000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Glucocorticoid Receptor antibody (#3660, Cell Signalling Technology) or control IgG antibody (#03-198, Merck Millipore) and incubated for 1 h at room temperature whilst rotating ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used in immunoblotting were as follows: rabbit polyclonal antibody anti-GAPDH (Merck Millipore #ABS16), mouse monoclonal antibody anti-PRKAR2B (#610625 ...
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bioRxiv - Plant Biology 2019Quote: ... The samples were fractionated using an Alliance 2795 HPLC system (Waters) and µLC column (SeQuant ZIC®-HILIC, 15 cm x 1 mm internal diameter, 5 µm, 200 Å, Merck), with a mobile phase consisting of solvent A (10 % 10 mM ammonium acetate pH 7.3 90 % acetonitrile ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Biochemistry 2021Quote: ... On the next day the buffer was exchanged to IEX-A buffer (25 mM Bis-Tris, 50 mM NaCl, 10 mM. β-mercaptoethanol) by ultra-filtration (Amicon Ultra-15 30 kDa, Merck Millipore) and subject to cation-exchange chromatography by a linear to IEX-B buffer (25 mM Bis-Tris ...