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Citations for Merck :
201 - 250 of 1283 citations for Recombinant Human Membrane Metallo Endopeptidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were separated by SDS–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride (PVDF) membrane (Hybond-P PVDF membranes, Merck, Germany) by a wet-transfer apparatus (Vep-3 ...
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bioRxiv - Neuroscience 2022Quote: ... from CNO and saline treated mice were first boiled at 95°C for 5 min and then separated on 10% polyacrylamide gel and transferred on PVDF membranes (Immobilon-P transfer membrane; Merck Millipore). Membranes were then blocked with 5% non-fat powdered milk in TBS + 0.01% Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C) and filtrated using membrane filters with a pore size of 0.45 μm (Millex® HV membrane filter units, Merck Millipore). The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of protein per sample was loaded and separated on 8-12% SDS–PAGE gels and electro blotted onto polyvinylidenedifluoride (PVDF) membranes (Immobilon-P PVDF membrane 0.45μm; Merck-Millipore, Burlington, USA). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently filtrated using membrane filters with a pore size of 0.45 μm (Millex® HV membrane filter units, Merck Millipore). The clarified lysate was supplemented with Glutathione Sepharose 4B (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membrane (PVDF Transfer Membrane, Merck-Millipore). The primary antibodies used for the detection of proteins are Anti-CRY1 (Bethyl) ...
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bioRxiv - Physiology 2021Quote: Human thrombin was purchased from Merck (Cramlington, UK) and reconstituted in double distilled water (ddH2O) ...
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bioRxiv - Microbiology 2021Quote: ... or polyclonal anti-human antibody (I2136, Merck, Germany) was coupled simultaneously in four flow cells ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-human nuclei (Merck, MAB4383, 1:250) were diluted in the blocking solution and incubated overnight on a shaker at 4◦C ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... human Ndc80 siRNA (HA12977117-004; Merck, Darmstadt, Germany), human ON-TARGET PRC1 siRNA (L-019491-00-0020 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% aprotinin and 0.1% Human Insulin Solution (Merck). Medium was changed every other day with EHT medium.
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human nuclear antigen (1:100; Merck), Alexa Fluor 647-conjugated rat anti-CD45 (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HTF medium (EmbryoMax Human Tubal Fluid; Merck Millipore) was mixed 1:1 with mineral oil (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Normal human epidermal keratinocytes were obtained from Merck. All lines were maintained in 3:1 DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... growth in LB and human serum (Merck #H4522) was measured ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with anti-human IFN-γ and anti-human IL2 antibodies for 40 min at RT in 0.2% saponin (Merck, Germany) in PBS (Gibco ...
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bioRxiv - Neuroscience 2020Quote: Recombinant GST-hSLP4A was expressed in and purified from BL21 Rosetta DE3 E.coli (Merck, Ca. No. 70954). 5ml of saturated pDEST15 hSlp4-a-transformed BL21 Rosetta DE3 E.coli culture were diluted in 500 ml 2x YT (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Cell Biology 2021Quote: ... 1000 units/ml of recombinant Leukemia Inhibitory Factor (LIF, Interchim, #8V6280) and 0.1% β-mercaptoethanol (Merck, #M6250) on gelatin-coated plates ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred to a PVDF membrane (Merck), which was blocked with 5% dry milk ...
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bioRxiv - Cell Biology 2019Quote: ... and transferred to PVDF membranes (Merck Millipore, Billerica, MA). All antibodies were diluted in Odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Developmental Biology 2020Quote: ... and transferred to Immobilon P membranes (Merck group, IVPD00010) using a semi-dry blotting system (Biometra/ Analytik Jena ...
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bioRxiv - Bioengineering 2022Quote: ... and electrotransferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). Membrane was cut between 80 and 110 kDa marker and at 50 kDa to probe simultaneously phospho or total MET ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Merck Millipore) for 1h at 20V ...
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bioRxiv - Molecular Biology 2019Quote: ... The sampes were filtered through nitrocellulose membrane (Merck-Millipore) layered on top of nylon Hybond N+ (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred to PVDF membranes (IPVH00010, Merck Millipore, Hertfordshire, UK) and immunoblotted with antibodies to phospho-S473 Akt (rabbit polyclonal ...
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bioRxiv - Molecular Biology 2019Quote: ... Elution was desalted on 0.025 µm VSWP membranes (Merck) against water for EMSAs.
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bioRxiv - Microbiology 2019Quote: ... and transferred to methanol-activated PVDF membranes (Merck, IPVH00010), using Western blot transfer buffer (3.03 g Tris ...
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bioRxiv - Biochemistry 2021Quote: ... 0.45 and 0.22-μm polyether sulfone membranes (Merck -Millipore) and finally diafiltered onto a polyether sulfone membrane with a 10kDa cut-off (Vivaspin ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electroblotted onto PVDF membranes (IPVH00010, Merck Millipore) via a semi- dry cassette (1703940 ...
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bioRxiv - Microbiology 2020Quote: ... and polyvinylidene difluoride (PVDF) membranes were obtained from Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... and transferred to nitrocellulose membranes (Merck Millipore, Ltd-Ireland) in Buffer Tris 25 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (0.45µm, Merck Millipore) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... PVDF membranes were stained with Ponceau S stain (Merck) to confirm full transfer followed by blocking for 30 minutes at room temperature in 5% non-fat milk in PBST (0.01% Triton-X100) ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred onto Immobilon-P membrane (Merck) using Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2022Quote: ... and transferred to polyvinylidene fluoride membranes (Merck Millipore, ISEQ00010). The membranes were blocked with 5% BSA in TBST for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... coated Millicell® culture membrane (Merck Millipore, Watford, UK) in submerged culture ...
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bioRxiv - Immunology 2020Quote: ... transferred to polyvinylidene fluoride membranes (Merck kGaA, Darmstadt, Germany), blocked using 5% bovine serum albumin for 1 h and incubated with diluted primary antibodies at 4 °C overnight ...
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bioRxiv - Genetics 2022Quote: ... and blotted onto polyvinylidene fluoride (PVDF) transfer membranes (Merck). Subsequently ...
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bioRxiv - Genetics 2022Quote: ... and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Membranes were blocked with 5% milk in Tris-buffered saline with tween (TBST ...
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bioRxiv - Cancer Biology 2019Quote: ... The membrane was detected using a TMB substrate (Merck). GAPDH (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... before being transferred onto 0.45 µm PVDF membrane (Merck). Immunostaining was performed by incubating membrane 1h at room temperature with 1/1000 diluted of rabbit polyclonal anti-Poly-ADP Ribose (TREVIGEN 4336-BPC-100) ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Membranes were blocked with 5% milk in Tris-buffered saline with tween (TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Membranes were incubated with 5% skimmed milk in PBS-T (PBS (Amresco ...
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bioRxiv - Physiology 2019Quote: ... and blotted on PVDF membranes (Immobilon-FL IPFL00010, Merck) for 1 hr at 100V on ice ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were blocked in 2% skim milk powder (Merck) and 0.5% FCS (Gibco ...