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Citations for Merck :
451 - 500 of 1283 citations for Recombinant Human Membrane Metallo Endopeptidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... using Pellicon 2 cassette with Biomax 300 kDa Membrane (Merck, USA).
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bioRxiv - Cell Biology 2023Quote: ... extracts were transferred to polyvinylidene fluoride (PVDF) membranes (Merck Millipore, German). Membranes were blocked with 5% defatted milk and then incubated with primary antibodies against anti-DNMT1 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Merck Millipore), followed by blocking in 2% BSA containing 50 mM Tris-HCl ...
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bioRxiv - Physiology 2022Quote: ... proteins were transferred to PVDF membranes (Merck Millipore, IEVH00005, Tokyo, Japan), blocked with 2.5% non-fat milk in PBS containing 0.1% Tween 20 for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... The microplate was covered with semi-permeable membrane (Breathe-Easy, Merck) and incubated at room temperature with 600 rpm shaking for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to a methanol pre-activated membrane (Merck, IPFL00010) and then blocked with 5% BSA (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then sealed with Breathe-Easy sealing membrane (Z380059, Merck) and incubated in the Biospa 8 (Biotek ...
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bioRxiv - Neuroscience 2023Quote: ... We transferred proteins onto Immobilion IF PDVF membrane (Merck Life Sciences) with NuPAGE transfer buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... separated by SDS-PAGE and electrophoretically transferred onto PVDF membranes (Merck). Membranes were blocked with 5% non-fat milk (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Immobilon-P PVDF membrane (IPVH00005) was purchased from Merck (Rehovot, Israel). BLUeye prestained protein ladder (PM007-0500 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred to a polyvinylidene difluoride membrane (Merck, Rahway, NJ, USA). The membrane was incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The slices were cultured on the membrane inserts (PICM0RG50; Merck, Ireland), placed on the culture medium (50% minimal essential medium ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked for 1 h with 5% skim milk (Merck) in TBST [50 mM Tris-HCL ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Neuroscience 2023Quote: ... the affinity resin was prepared by incubating 100 µg of recombinant protein with 20 µl of nickel beads (PureProteome Nickel magnetic beads, Merck) in 50 mM Pull-Down Buffer PDB (Tris–HCl pH 7.4 ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The dose of COS (75-400 IU daily of recombinant follicle stimulating hormone (Gonal-F Merck or hMG Menopur, Ferring) was individualized to patient’s age ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... Ovarian follicle growth tracking was performed by scheduled transvaginal ultrasound examinations that lead to the planning timepoint for oocyte maturation triggered by administration of recombinant hCG (250 microgr SC Ovitrelle; Merck). Oocyte retrieval was carried out by transvaginal ultrasographically-guided follicular puncture 37 hours later.
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bioRxiv - Biochemistry 2024Quote: ... Affinity-purified recombinant TrK13 proteins (WT, C580Y, R539T and C580Y) or commercially purchased BSA (A7030) or lysozyme (L6876) (Merck, Germany), each at 10 µM concentration ...
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bioRxiv - Cell Biology 2021Quote: Human immortalised articular chondrocyte C28/I2 cells were purchased from Merck Millipore and cultured in DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: The human brain microvascular endothelial cell line hCMEC/D3 (Merck Millipore) was seeded in T25 flasks coated with rat tail type I collagen (Merck Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... When stained for human nuclei (MAB1281, Merck Millipore, Fig. 4C-D), this was performed for 30 min at RT in the dark after EdU staining (Click-iT Assay ...
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bioRxiv - Microbiology 2020Quote: ... and affinity-purified rabbit polyclonal antibody against human CPSF6 (HPA039973; Merck) were used at a dilution of 1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: The HCYTOMAG-60K-15 Human Cytokine/Chemokine Multiplex Assay (Merck Millipore) was employed to simultaneously analyze TNF-α ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 IU mL1 human chorionic gonadotropin (hCG; Chorulon; Merck Animal Health) and 1.0 mg mL1 oestradiol ...
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Developmental Biology 2023Quote: ... Coating was performed with 20 µg/ml human fibronectin (Merck FC010) in PBS for at least 30’ ...
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Lowering mutant huntingtin by small molecules relieves Huntington’s disease symptoms and progressionbioRxiv - Molecular Biology 2023Quote: ... and anti-eIF4G1 for human samples (1:2000; 07-1800, Merck) or anti-eIF4G3 for mouse samples (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and antibodies directed against anti-human mitochondria (1:100, Merck #1273;), CDK2AP1 (rabbit ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore, MA, USA), anti-Ku80 (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... or 14% SDS-PAGE gel and transferred onto PVDF membrane (Merck, ISEQ85R) using a semi-dry transfer unit (Bio-Rad Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... then transferred onto polyvinylidene fluoride membrane (IPVH00010, Merck Millipore, Billerica, MA, USA), blocked with 5% dry nonfat milk in Tris-buffered saline (pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... separated by SDS-page and blotted onto a polyvinylidene difluoride membrane (Merck) by using a Wet/Tank Blotting System (Biorad) ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were blocked with 10% (W/V) skim milk (Merck, USA) and subsequently incubated with primary antibodies for 2 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred from the gel to a PVDF membrane (Merck, IPFL00010) by semi-dry transfer with Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were filtered through nitrocellulose (NC)-filter membranes (Merck Millipore HAWP 0025) at a rate of 7.5mL/min ...
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bioRxiv - Molecular Biology 2021Quote: ... WB was performed with homemade SDS-PAGE gels and nitrocellulose membranes (Merck). The following antibodies were used ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to an Immobilon-FL PVDF membrane (Merck, Amsterdam, the Netherlands). The membrane was blocked for 1 hour at room temperature in 4% skim milk/PBS containing 0.1% Tween-20 ...
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bioRxiv - Physiology 2020Quote: ... proteins were transferred to a PVDF membrane (Immobilon-P, Merck Millipore, IPVH00010) with 160 mA for 90 min ...
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bioRxiv - Microbiology 2019Quote: ... onto polyvinylidene fluoride membranes (Immobilon-P, 0.45 μm; Merck-Millipore, Darmstadt, Germany). Membranes were probed using anti-HA or anti-SiiA and anti-SiiB primary antibodies (as indicated in the respective figures ...
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bioRxiv - Neuroscience 2020Quote: ... and transferred to Immobilon-FL PVDF membrane (0.45 μm pore; Merck, IPFL00010). Membranes were incubated with 0.5% casein (Bio-rad ...
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bioRxiv - Biophysics 2021Quote: ... Separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck) and incubated for 2hr with blocking buffer containing 5% Skimmed milk (70166 ...
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bioRxiv - Cell Biology 2021Quote: ... blotted onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) via semi-dry blotting system and protein signal analyzed by using enhanced chemiluminescence to detect bound antibodies (WesternBrightTM ECL ...
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bioRxiv - Biochemistry 2021Quote: ... After transferring onto a polyvinylidene fluoride (PVDF) membrane (Merck KGaA, Darmstadt, Germany), bands on the membrane were visualized using Chemi-Lumi One Ultra (NACALAI TESQUE ...
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bioRxiv - Systems Biology 2020Quote: ... each well of the 96-well PVDF membrane plates (MSIPS4510, Merck Millipore) was activated and equilibrated with 150 μl of 70% ethanol/water and urea sample solution ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred to PVDF Immobilon-P transfer membrane (Merck-Millipore, Darmstadt, Germany) as previously described (32) ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Microbiology 2020Quote: ... electrotransfered onto 0.2-µm Immobilon®-PSQ PVDF membrane (ISEQ00010, Millipore/Merck) and immunoblotting was done essentially as described above ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred onto the methanol-activated µm PVDF membrane (Merck #ISEQ85R) using 1X transfer buffer (2.5 mM Tris-HCl pH 7.5 ...