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Citations for Merck :
551 - 600 of 1283 citations for Recombinant Human Membrane Metallo Endopeptidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
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bioRxiv - Biochemistry 2020Quote: ... 500 μM ATP and ~0.5 μg commercially available human CyclinB-Cdk1 (Merck). The reaction was carried out at 30 °C and was stopped using SDS-PAGE sample buffer after 5 minutes.
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bioRxiv - Cell Biology 2019Quote: ... Human pancreatic organoids were additionally immuno-stained against Sox9 (primary antibody: Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... a rabbit anti-human EGFR monoclonal antibody (EMD Millipore-MERCK, Temecula, USA) diluted 1:1000 or a rabbit anti-human VEGF-A polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... human tau constructs were expressed in the vector pNG2 (Merck-Novagen, Darmstadt) in E ...
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bioRxiv - Immunology 2021Quote: ... MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) were used ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 501µg/ml Ascorbic acid and 180⍰µg/ml Human transferrin (Merck, T8158)) and then plated onto non-adherent dishes (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3 Merck Life Science) were cultured on 250µg/ml collagen-I (Advanced Biomatrix ...
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bioRxiv - Developmental Biology 2023Quote: ... and reinjected after 48 hours 7.5IU hCG (human chorionic gonadotrophic hormone; Merck), before overnight mating with F1 or F1 hybrid transgenic mT/mG stud males (original mT/mG transgenic mouse line obtained from Jackson Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... A solution was prepared by mixing 70 µL human plasma fibronectin (Merck), 20 µL green fibrinogen and 910 µL PBS ...
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bioRxiv - Microbiology 2024Quote: ... Human Serum Albumin with or without fatty acids were purchased from Merck. IVIg (Multigam® 5%) ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... urinary human menopausal gonadotropin (hMG) (HMG-lepori 75 UI, Angelini Farmacéutica, S.A, Spain) and recombinant hCG (Ovitrelle 250 micrograms, Merck Serono Europe Limited, Spain) or luprorelin acetate (Procrin 1 mg/0,2 ml ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were transferred onto an Immobilon FL PVDF membrane (Merck Millipore, Darmstadt, Germany), probed with antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated with primary antibodies against NKX2.1 (1:200; Merck, 07-601), proSFTPC (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... separated by electrophoresis and transferred to polyvinylidene difluoride membranes (0.2 μm; Merck Millipore). The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... and transferred to a polyvinylidene difluoride membrane (PVDF; Merck Millipore Ltd., Tullagreen, Ireland) that was blocked in Licor Blocking Buffer diluted 1:1 in 0.1M PB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Biochemistry 2019Quote: ... the membrane was developed using Luminata Classico Western HRP substrate (Merck Millipore, USA) and detected in ImageQuant LAS500 imager (GE Life Science ...
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bioRxiv - Neuroscience 2019Quote: ... and electro transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, EUA). After blocking with 5% bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Molecular Biology 2019Quote: ... the RNA samples were transferred to a positively charged nylon membrane (Merck, #11209299001) and were hybridized with probes at a concentration of 1 µg/ml in DIG Easy Hyb (Merck ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Microbiology 2021Quote: ... then transferred to an Immobilon-FL PVDF membrane (Merck Millipore Ltd., Tullagreen, Ireland) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a PVDF membrane (Immobilon-FL; Merck Millipore, Billerica, MA). Immunoblotting was performed with primary antibodies against Camk2a phosphorylated at pT286 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred onto low fluorescent Immobilon-FL membrane (0.45μm por size, Merck) using the Biorad wet-blot system (1.15h ...
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bioRxiv - Molecular Biology 2022Quote: ... The resolved proteins were transferred onto a 0.45 m PVDF membrane (Immobilon, Merck) in ice-cold transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... and a well insert with a 0.78 cm2 polycarbonate membrane (Merck Millipore, #PIHP01250) coated with bovine collagen I (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then transferred to a prewet immobilon PVDF transfer membrane (Merck Millipore Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... Protein transfer to PVDF membranes (Immobilon-P, 0.45 µm pore size, IPVH00010, Merck) was achieved using a wet transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... An additional step of filtration using a 0.45 μm membrane filter (Merck Millipore) was used to remove any remaining yeast cells or debris ...
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bioRxiv - Microbiology 2021Quote: ... The crude extract was filtered through a 0.22 μm membrane filter (Merck Millipore) and loaded on a disposable purification column packed with TALON Superflow Resin (Clontech) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins separated in the gel were transferred to a PVDF membrane (Merck Millipore) using a submerged blotting apparatus and then visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Microbiology 2022Quote: ... Gels were either Coomassie-stained or used for immunoblotting employing PVDF membranes (Merck) as described above ...
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bioRxiv - Plant Biology 2019Quote: ... then transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). Western blotting was carried out using mouse anti-GFP 11814460001 (Roche ...
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bioRxiv - Physiology 2020Quote: ... separated on SDS polyacrylamide gel (12%) and transferred to a PVDF membrane (Merck). After 1 h blocking (Odyssey blocking buffer with 0.1% Tween20) ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were transferred from gel to a PVDF membrane (Merck, cat. no. IPSN07852). Transfer was carried out for 45 minutes at 110V in transfer buffer (cold room) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was filtered using a Millipore membrane filter 0.45 μm (Merck), and checked for sterility by plating onto Sabouraud’s agar and into BHI broth with incubation at 30°C for seven days.
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bioRxiv - Neuroscience 2020Quote: ... and proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Chengdu, China) for 2 h at 150 mA in 10% transfer buffer [3% Tris–HCl (Aladdin Industrial Corporation ...
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bioRxiv - Immunology 2020Quote: ... Protein was transferred to PVDF membranes (Immobilon-P PVDF .45um, Merck Chemicals BV). Membranes were blocked in 5% fat-free milk (Elk ...
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bioRxiv - Molecular Biology 2019Quote: ... The membranes were stripped using a 1x ReBlot Strong Antibody Stripping Solution (Merck) for 15 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... After blotting the DNA on a nylon membrane (Hybond N+, Merck, Darmstadt, Germany) it was cross-linked to the membrane in a Stratalinker (Stratagene ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was then incubated with rabbit anti-CSF1R antibody (1:1500, Merck), or rabbit anti-GFP (1:40,000 ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant collected was filtered through the 0.45 μm PVDF membrane (Millipore, Merck). The pre-packed Ni-NTA affinity column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... followed by filtration with a 0.45-μm-pore-size HAWP membrane (Merck Millipore). Ten liters of seawater samples were preacidified as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... eDNA was captured using 0.45 μm Sterivex filter units (PVDF membrane, Merck Millipore) connected to a peristaltic pump (Easy Load II Peristaltic Pump ...
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bioRxiv - Microbiology 2021Quote: ... Filtration was performed with 0.22-μm Durapore membrane filters (Merck KGaA, Darmstadt, Germany) after prefiltration with 5 μm Durapore membrane filters (Merck KGaA ...
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bioRxiv - Microbiology 2021Quote: ... samples were filtered through a 0.1-μm Omnipore™ membrane filter (Merck Millipore) using a 90 mm diameter stainless-steel filter holder (Merck Millipore ...