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Citations for Merck :
401 - 450 of 593 citations for Recombinant Human Complement Factor H His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Customised 7-plex Panel (Merck Millipore) was used to quantify cytokine levels.
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bioRxiv - Bioengineering 2021Quote: ... the chemically decellularized aortas were treated with 900 units (25 μg/mL DNase I recombinant, RNase free, Cat. No. 4716728001, Roche, Merck KGaA, Darmstadt, Germany) using a total of 3 DNase treatment for 2 hours each to achieve the desired level of DNA assessed by (Quant-iT™ PicoGreen™ dsDNA Assay Kit ...
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bioRxiv - Molecular Biology 2021Quote: Transfected HEK293T cells were treated for 16 h with DMSO or with 10 µM Cyclopiazonic acid (CPA, Merck) before collection.
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bioRxiv - Genomics 2019Quote: The populations were enriched in metaphasic cells by 2 h of treatment with 200 nM nocodazole (Merck, M1404) prior to cell recovery ...
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bioRxiv - Cell Biology 2021Quote: ... After 24 h cells were stained for 30 min at 37 °C with NAO dye (200 nM, Merck), washed ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The H and E-stained sperms on the slides were mounted using Dibutylphthalate Polystyrene Xylene (DPX) (Merck, Germany) and dried overnight at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Total protein was extracted from the cells 48 h post-transfection by complete lysis-M buffer (Merck, USA). Twenty-five mg of total protein were loaded per well in a 12% SDS PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... Proteasome complexes were subsequently blotted for 1 h at 200 V on ice onto PVDF membranes (Merck Millipore) and further handled as described above.
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bioRxiv - Microbiology 2021Quote: ... Mycelia cultured for 24 h in CD medium were collected by filtering through Miracloth (Merck Millipore, Darmstadt, Germany), washed with water ...
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bioRxiv - Microbiology 2021Quote: ... and infected with pseudovirus for 24 h in the presence of polybrene (10 μg/ml, Merck, Darmstadt, Germany). BMMs were washed with PBS for three times at 24 h after infection ...
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bioRxiv - Cell Biology 2022Quote: E10.5 embryos were fixed in 4% PFA/PBS for 1.5 h followed by incubation in 15% sucrose (Merck)/PBS for 2 h and overnight incubation in 30% sucrose/PBS ...
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bioRxiv - Microbiology 2022Quote: ... An aliquot of 5 μl was spotted on an activated (100 °C, 1 h) TLC sheet (Merck, USA). CM2 ...
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bioRxiv - Cell Biology 2023Quote: ... After 23 h the cells were detached and reseeded on glass cover slips coated with Concanavalin A (Merck, Darmstadt ...
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bioRxiv - Molecular Biology 2023Quote: ... Following saturation for 1 h in PBS 1X containing 5% milk and 0.1% Tween 20 (655204-100ML, Merck), the membranes were incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... cryosections were incubated at RT for 1 h in darkness with the secondary antibody and Hoechst 33258 (Merck) as a nuclear counterstain ...
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bioRxiv - Immunology 2024Quote: ... plates were washed in PBS/T and incubated for 1 h with goat anti-mouse IgG-AP (Merck). Plates were then developed as per the mAb ELISA ...
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bioRxiv - Developmental Biology 2024Quote: ... sections were mounted with Vecta-Shield (Vector #H-1000) or Mowiol (Roth #0713.2) optionally containing DAPI (Merck #1.24653).
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bioRxiv - Neuroscience 2021Quote: ... Anti-human Aβ mouse monoclonal antibody (clone W0-2; Merck Chemicals GmbH, Darmstadt, Germany) and rabbit anti-β-actin monoclonal antibody (clone 13E5 ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeding on 16.7 µg/ml human plasma fibronectin-coated plates (Merck Millipore, FC010). After 48h ...
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bioRxiv - Bioengineering 2021Quote: ... Human plasminogen-depleted fibrinogen was utilized for hydrogel preparation when stated (Merck, Darmstadt, Germany). Aprotinin was purchased from R&D Systems (Minneapolis ...
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bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... Mission siRNA for human Pacsin 2 siRNA (SASI_Hs01_0021-5538, SASI_Hs01_0021-5539 and SASI_Hs01_0021-5540, Merck) or MISSION siRNA Universal Negative Control #1 (SIC-001 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Calibration curve samples were generated by diluting the International Standard with human plasma (Merck) to create a standard curve ranging from 955 000 IU/mL to 95.5 IU/mL with three technical replicates of each concentration ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human kindlin2 (mouse monoclonal, 3A3, Merck, MAB2617; WB 1:1000, IF 1:200), anti-mouse kindlin2 (rabbit polyclonal ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... injection of 5 IU of human chorionic gonadotrophin (hCG; Pregnyl, Merck, Kilsyth, VIC, Australia) 48 h later.
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bioRxiv - Biochemistry 2021Quote: ... and soluble protein was clarified by centrifugation (50,000×g, 1 h, 4°C) and subsequently passed through a 0.22-μm filter (Merck Millipore).
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bioRxiv - Biophysics 2021Quote: ... The culture supernatant was collected after 72 h and concentrated using Amicon Ultra-15 MWCO 100 kDa (Merck Millipore).
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bioRxiv - Cell Biology 2021Quote: ... approximately 1x 106 cells of each cell line were spin-infected for 1 h at 1.9 × 103 g (3000 rpm) in the presence of 10 μg/mL polybrene (Merck) to facilitate the infection ...
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... The reactions were incubated at 37 °C for 1 h and subsequently stopped by addition of 1x PhosStop (Merck). In vitro phosphorylation of dephosphorylated recombinant SYNJ2BP (0.5 µg in 10 µl ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored for 24 h at 4°C in 4% PFA and then washed and stored in PBS containing 0.05% sodium-azide (Merck). Within a week of perfusion ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were post-fixed for 2 h at RT in 1% osmium tetroxide in 0.05 M cacodylate buffer pH 7.4 (Merck, Germany) and dehydrated stepwise in a graded ethanol series followed by 100% acetone ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer was done using the Bio-Rad wet-blotting system at 500 mA for 1 h on 100% methanol-activated low-fluorescent Immobilon-FL membranes (0.45 μm pore size, Merck). The blot transfer buffer consisted of 25 mM Tris ...
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bioRxiv - Microbiology 2023Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... and then blocked for 1 h at RT with 0.2% Triton-X in PBS + 1% normal goat serum (NGS, Merck). Antibodies used and their concentration is found in Supplementary Table 4 ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR or EdU proliferation assay) for 72 h in the presence or absence of 200 nM Smoothened agonist (Merck). Full medium changes were performed daily ...
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bioRxiv - Bioengineering 2021Quote: ... Part A consisted of 20% hPL (v/v) and 4mg/mL human fibrinogen (Merck, USA) in DMEM/F12 (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Molecular Biology 2021Quote: ... and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer; Merck). Finally ...
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bioRxiv - Immunology 2021Quote: ... MIP-1β by MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Microbiology 2023Quote: ... and NTK seeded on surfaces coated with 100 μg/ml type IV human collagen (Merck) in defined keratinocyte serum-free medium (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... All human cell lines were cultured at 37℃ in 5% CO2 in DMEM media (Merck) containing 1% antibiotic (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Primers for human H19 were used as a positive control for CTCF binding (Merck Millipore).
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bioRxiv - Immunology 2023Quote: ... cells were cultured in RPMI 1640 (ccpro) supplemented with 10 % heat-inactivated human serum (Merck), 50 U/ml IL-2 (Miltenyi) ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted with the following antibodies: anti-human PSEN1-CTF (MAB5643; Merck Millipore); anti-PEN2 (D6G8 ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells (ATCC) and FibroGRO Xeno-Free human foreskin fibroblasts (Merck, gift from Martin Lowe) were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2021Quote: Viability of SH-SY5Y cells after treatments with or without H-LIPEF was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...